Clinical Outcomes of the Double Lateral Sliding Bridge Flap Technique with Simultaneous Connective Tissue Graft in Sextant V Recessions: Three-Year Follow-Up Study

The presence of isolated or multiple gingival recessions in the mandibular anterior region is a challenge for the clinician, as they may be associated with a shallow vestibule, high frenum insertion and/or little or no attached gingiva. Only limited evidence is available on the use of the double lateral sliding bridge flap technique with connective tissue graft (CTG) technique for treating gingival recessions in the mandibular anterior region. The aim of this study was to describe and evaluate the clinical and esthetic outcomes of the double lateral sliding bridge flap technique with CTG on isolated and multiple gingival recessions at the level of the mandibular incisors. Nine patients required treatment of gingival recessions in the mandibular incisors at the University of Salamanca (Spain) (seven females, two males; mean age: 27.9 ± 6.9) with a total of 14 isolated (42.9%) and multiple (57.1%) Miller class II and III gingival recessions. After a mean follow-up of 36 months, the mean percentage of root coverage was 80.5% for all treated recessions. Statistically significant differences (p < 0.05), were observed for reduction in recession depth, increased width of keratinized tissue and increased gingival thickness, this being dependent on the Miller class. The esthetic outcome was acceptable, with a final mean esthetic score of 7.4 out of 10. The double lateral sliding bridge flap surgical technique with CTG is an effective procedure for the coverage of isolated and multiple gingival recessions in the anterior mandibular region, as it offers satisfactory esthetic results

Soil greenhouse gas emissions and crop production with implementation of alley cropping in a Mediterranean citrus orchard

The implementation of alley cropping in orchards has been suggested as a sustainable strategy to increase farmer revenues by crop diversification, enhance soil organic matter (SOM) and fertility, water retention, overall biodiversity, and contribute to climate change mitigation. Thus, the objective of this study was to assess if alley cropping with annual crops can contribute to i) mitigate soil greenhouse gas (GHG) emissions, ii) enhance C sequestration in a semiarid Mediterranean irrigated citrus orchard, and iii) increase land productivity. For this, two different treatments were established: i) conventional mandarin monoculture (MC) with no alley cropping; and ii) mandarin diversified with alley cropping of barley/vetch and fava bean (DIV). Measurements of soil CO2 and N2O emissions were periodically performed (every 7–20 days) during two years. Soil CO2 emission rates followed the soil moisture trend, and showed no significant differences between treatments. As an average, soil CO2 emission rates were 147 mg m-2 h-1 in MC and 196 mg m-2 h-1 in DIV. Soil N2O emission rates were not correlated to soil moisture nor temperature, and showed average values of 0.026 mg m-2 h-1 in MC and − 0.002 mg m-2 h-1 in DIV. Alley cropping did not contribute to significantly increase soil organic C and total nitrogen in two years’ time. With regard to production, mandarin yield showed no significant differences between treatments, but alley crops contributed to complementary commodities to the main cash crop, increasing overall land productivity. Thus, alley cropping in irrigated Mediterranean orchards has no significant effect on soil C sequestration and GHG emissions at short-term, with increased land productivity owing to new commodities grown in the alleys. These results confirm that under semiarid Mediterranean climate, long periods are needed to efficiently assess soil C sequestration potential of sustainable practices in orchards.This work was supported by the European Commission Horizon 2020 Project Diverfarming [Grant agreement 728003]. Raúl Zornoza acknowledges the financial support from the Spanish Ministry of Science, Innovation and Universities through the “Ramón y Cajal” Program [RYC-2015-18758]

Beneficial Effect of Short-Term Supplementation of High Dose of Vitamin D3 in Hospitalized Patients With COVID-19: A Multicenter, Single-Blinded, Prospective Randomized Pilot Clinical Trial.

There is now sufficient evidence to support that vitamin D deficiency may predispose to SARS-CoV-2 infection and increase COVID-19 severity and mortality. It has been suggested that vitamin D3 supplementation may be used prophylactically as an affordable and safe strategy that could be added to the existing COVID-19 standard treatment. This multicenter, single-blinded, prospective randomized pilot clinical trial aimed to evaluate the safety, tolerability, and effectiveness of 10,000 IU/day in comparison with 2000 IU/day of cholecalciferol supplementation for 14 days to reduce the duration and severity of COVID-19 in 85 hospitalized individuals. The median age of the participants was 65 years (Interquartile range (IQR): 53-74), most of them (71%) were men and the mean baseline of 25-hydroxyvitamin D (25(OH)D) in serum was 15 ng/ml (standard deviation (SD):6). After 14 days of supplementation, serum 25(OH)D levels were significantly increased in the group who received 10,000IU/day (p < 0.0001) (n = 44) in comparison with the 2,000IU/day group (n = 41), especially in overweight and obese participants, and the higher dose was well tolerated. A fraction of the individuals in our cohort (10/85) developed acute respiratory distress syndrome (ARDS). The median length of hospital stay in these patients with ARDS was significantly different in the participants assigned to the 10,000IU/day group (n = 4; 7 days; IQR: 4-13) and the 2,000IU/day group (n = 6; 27 days; IQR: 12-45) (p = 0.04). Moreover, the inspired oxygen fraction was reduced 7.6-fold in the high dose group (p = 0.049). In terms of blood parameters, we did not identify overall significant improvements, although the platelet count showed a modest but significant difference in those patients who were supplemented with the higher dose (p = 0.0492). In conclusion, the administration of 10,000IU/day of vitamin D3 for 14 days in association with the standard clinical care during hospitalization for COVID-19 was safe, tolerable, and beneficial, thereby helping to improve the prognosis during the recovery process.This work was supported by Fundación Universidad Alfonso X el Sabio (FUAX, Madrid, Spain; ID Project: 1.012.010; ID Project EQA: 925.280); the Coordinated Research Activities at the National Center of Microbiology (Instituto de Salud Carlos III) (COV20_00679) to promote an integrated response against SARS-CoV-2 in Spain (Spanish Ministry of Science and Innovation) that is coordinated by Dr. Inmaculada Casas (WHO National Influenza Center of the CNM); a generous donation provided by Chiesi España, S.A.U. (Barcelona, Spain). The work of Montserrat Torres was financed by the Coordinated Research Activities at the CNM (Instituto de Salud Carlos III) (COV20_00679). The work of Lorena Vigón was supported by a pre-doctoral grant from Instituto de Salud Carlos III (FIS PI16CIII/00034-ISCIII-FEDER). The work of Sara Rodríguez-Mora was financed by NIH grant R01AI143567.S

PE0306 Haplotype-Resolved Genome Assemblies and a Pan-Genome of Six Pistachio Cultivars

Trabajo presentado en la International Plant and Animal Genome Conference, celebrada en San Diego, CA (Estados Unidos), del 13 al 18 de enero de 2023Pistachio (Pistacia vera L., 2n =2x = 30) is one of the most economically important long-lived crops, including cashew and mango in the family Anacardiaceae. Although dioecious pistachio trees are xerophytic and highly resilient to abiotic stresses, they require a certain amount of winter rest period to exit bud dormancy and to flower synchronically between male and female trees. The `Kerman¿ (P. vera cv. Kerman) and `Peter¿ (P. vera cv. Peter) have been the dominant pair of female and male cultivars traditionally grown in California for more than 50 years, whereas the understanding of genomic diversity and approaches for pistachio breeding is limited without the reference-quality genome. Genetic diversity, such as structural and gene content variation, is a major source for crop improvement and breeding, which cannot be captured from a single reference genome. In the present study, we report haplotype-resolved chromosome-level reference genomes of six genetically diverse collections of pistachio (`Kerman¿, `Mateur¿, `Sirora¿, Napoletana¿, `Chaparrillo¿, and `T41¿). The diploid assemblies ranged from 568.55 to 608.21 Mb in size with contig N50 of 28 - 37 Mb. More than 98% of conserved orthologs (BUSCO) were represented for all genome assemblies. The dynamics of transposable elements (TEs) were characterized in the genomes where the TEs accounted for about 65 - 68% of the genomes. The gene annotation analyses using Iso-seq data generated from different five tissue types predicted about 38,000 protein-coding genes. Two haplotypes of each genome showed substantial sequence variation, suggesting high heterozygosity in pistachio genomes. Pan-genome construction and analyses characterized remarkable presence-absence gene content variation between cultivars. The reference genomes with fully annotated genes and pan-genome construction serve as rich resources for genomic breeding and improvement and comparative genomics of pistachio

EVALUACIÓN DE FIREWALLS BASADOS EN SOFTWARE LIBRE (FIREWALL EVALUATION BASED ON OPEN SOURCE SOFTWARE)

En este trabajo se propone la evaluación de firewalls basado en software libre GNU/LINUX con las características que les permitan integrarse a una cama de pruebas en la cual se estudiaron, evaluaron y analizaron diversos entornos de red y escenarios de ataque. Los firewalls de software libre son una buena alternativa cuando se trata de brindar seguridad en una red, en este trabajo fueron evaluados cuatro distribuciones Linux que han sido especialmente diseñadas para brindar este servicio. Las herramientas de software libre empleadas demostraron ser las adecuadas para las pruebas realizadas a los diferentes firewalls, de este modo se logró obtener resultados que muestran la factibilidad de ClearOS para ser implementado en la cama de pruebas. También se probó su efectividad al mostrar una buena respuesta en la defensa de los ataques, con y sin la inyección del tráfico de fondo generado por la herramienta iperf.In this work we propose the evaluation of firewalls based on free software GNU / LINUX with the characteristics that allow them to be integrated into a test bed in which various network environments and attack scenarios were studied, evaluated and analyzed. Free software firewalls are a good alternative when it comes to providing security in a network, in this work were evaluated four Linux distributions that have been specially designed to provide this service. The free software tools used, proved to be adequate for the tests carried out on the different firewalls, in this way it was possible to obtain results that show the feasibility of ClearOS to be implemented in the test bed. Its effectiveness was also proven by showing a good response in the defense of attacks, with and without the injection of background traffic generated by the iperf tool

Soil greenhouse gas emissions and crop production with implementation of alley cropping in a Mediterranean citrus orchard

The implementation of alley cropping in orchards has been suggested as a sustainable strategy to increase farmer revenues by crop diversification, enhance soil organic matter (SOM) and fertility, water retention, overall biodiversity, and contribute to climate change mitigation. Thus, the objective of this study was to assess if alley cropping with annual crops can contribute to i) mitigate soil greenhouse gas (GHG) emissions, ii) enhance C sequestration in a semiarid Mediterranean irrigated citrus orchard, and iii) increase land productivity. For this, two different treatments were established: i) conventional mandarin monoculture (MC) with no alley cropping; and ii) mandarin diversified with alley cropping of barley/vetch and fava bean (DIV). Measurements of soil CO2 and N2O emissions were periodically performed (every 7–20 days) during two years. Soil CO2 emission rates followed the soil moisture trend, and showed no significant differences between treatments. As an average, soil CO2 emission rates were 147 mg m-2 h-1 in MC and 196 mg m-2 h-1 in DIV. Soil N2O emission rates were not correlated to soil moisture nor temperature, and showed average values of 0.026 mg m-2 h-1 in MC and − 0.002 mg m-2 h-1 in DIV. Alley cropping did not contribute to significantly increase soil organic C and total nitrogen in two years’ time. With regard to production, mandarin yield showed no significant differences between treatments, but alley crops contributed to complementary commodities to the main cash crop, increasing overall land productivity. Thus, alley cropping in irrigated Mediterranean orchards has no significant effect on soil C sequestration and GHG emissions at short-term, with increased land productivity owing to new commodities grown in the alleys. These results confirm that under semiarid Mediterranean climate, long periods are needed to efficiently assess soil C sequestration potential of sustainable practices in orchards.This work was supported by the European Commission Horizon 2020 Project Diverfarming [Grant agreement 728003]. Raúl Zornoza acknowledges the financial support from the Spanish Ministry of Science, Innovation and Universities through the “Ramón y Cajal” Program [RYC-2015-18758].Peer reviewe

Protein Identification and Haplotype Description of Homozygote Mutation Causing Congenital Plasminogen Deficiency

Severe type I Plasminogen (PLG) deficiency was clinically diagnosed after hyaline-positive periodic acid Schiff material was detected in the histologic study of superior tarsal conjunctiva and vulvar pseudomembrane of the patient. Direct immunofluorescence also confirmed multiple deposits of fibrinogen in the dermis. Plasma plasminogen activity was calculated in a <5% value (reference values, 75% to 150%) and sequencing of the PLG gene evidenced the homozygous mutation in c.2377T/A (p.Tyr793Asn), confirming the molecular diagnosis of congenital deficiency of plasminogen type 1. Genotype-Phenotype correlation among family members evidenced the recessive hereditary pattern of clinical manifestations of chronic inflammatory disease of the mucous membranes due to PLG deficiency, but co-dominance effect to present a decreased plasma plasminogen activity (46%) among heterozygous asymptomatic individuals. SNPs/CNVs whole genome array hybridization analysis in the patient, detected long Loss of Heterozygosity regions (LOH) and demonstrated the consanguinity in the family. Proteomic analysis identified impaired secretion of mutant PLG tissue specific proteins, as definitive molecular etiopathogenesis of the type I PLG deficiency in the patient

Eye Movement Desensitization and Reprocessing desde la Propuesta de Valoración de Mario Bunge

The practice of psychological intervention should be based on the best scientific evidence about its efficacy. The objective of this study was to analyze the attitude, validity and scientific domain possessed by the psychotherapy of Eye Movement Desensitization and Reprocessing (EMDR) based on a scientific realism approach proposed by Bunge in his comparison table of scientific attitudes and pseudoscientific. The methodological design included linguistic and cultural adaptation, content validation with the criteria of judges, from the Bunge table; the bibliographic search of articles between 2016-2020, in the Scopus and Web of Science databases, with experimental and quasi-experimental study designs related to EMDR. The findings evidenced 2417 publications, 38 of which meet the criteria indicated, where it is indicated that EMDR reflects the criteria to be considered a science, sustaining its effectiveness.La práctica de la intervención psicológica debería estar basada en las mejores evidencias científicas acerca de su eficacia. El objetivo de este estudio fue analizar la actitud, validez y dominio científico que posee la psicoterapia de la Desensibilización y Reprocesamiento por los Movimientos Oculares (EMDR) con base en un enfoque de realismo científico propuesto por Bunge en su tabla de comparación de las actitudes científicas y pseudocientíficas. El diseño metodológico incluyó la adaptación lingüística y cultural, la validación de contenido con el criterio de jueces, de la tabla de Bunge; la búsqueda bibliográfica de artículos entre 2016-2020, en las bases de datos de Scopus y Web of Science, con diseños de estudio experimentales y cuasiexperimentales relacionados al EMDR. Los hallazgos evidenciaron 2417 publicaciones, 38 de las cuales cumplen con los criterios señalados, en donde se indica que la EMDR reflejan los criterios para considerarse una ciencia, sosteniendo su eficacia

I.amAble: la ciencia (química) al alcance de toda la sociedad

En este proyecto de innovación, que nace con vocación de continuar en años sucesivos, se persigue mejorar la calidad de la formación de los estudiantes de la Facultad de Ciencias Químicas (F. CC.QQ.) en el ámbito de la docencia teórico-práctica y de la divulgación científica. El trabajo ha consistido en la preparación de unos experimentos prácticos para llevarlos a cabo en centros educativos no universitarios en los que se ha tenido en cuenta la participación conjunta de personas con y sin diversidad funcional, desde una perspectiva inclusiva colaborativa. Estas actividades las han realizado los estudiantes bajo la supervisión de profesores (PDI) y personal de administración y servicios (PAS). Los experimentos se han recogido en fichas didácticas para facilitar su desarrollo y aplicación por parte de otros usuarios. En estas fichas se explica detalladamente cómo realizar las experiencias en formato de taller. Las fichas de los talleres realizados están disponibles en una página web vinculada a la Universidad Complutense bajo el título I.amAble (iamable.ucm.es). Está página ha sido construida por un estudiante de la Facultad de Informática , bajo la supervisión de profesionales, tanto de esa facultad como del Instituto de Tecnología del Conocimiento, y está abierta a contribuciones similares de otras facultades y otras instituciones. La página web está diseñada de manera que resulte lo más intuitiva y accesible posible para todo tipo de público. Entre todos los experimentos se han elegido cuatro para llevarlos a la práctica en centros educativos como actividades inclusivas en las que han participado conjuntamente personas con y sin discapacidad. Con este proyecto se pretende mejorar la calidad docente al ofrecer a los estudiantes la posibilidad de aprender enseñando mediante una actividad semipresencial. El desarrollo por parte de los estudiantes de competencias transversales en educación y en divulgación de la ciencia facilitarán algunas salidas profesionales en el ámbito educativo formal (centros de enseñanza) o informal (museos, animación sociocultural). Otro aspecto importante a resaltar es la potenciación de la colaboración entre todos los miembros de la institución universitaria. Este proyecto pretende contribuir a la mejora de la cultura científica, así como al establecimiento de puentes entre la UCM y la sociedad a la que debe servir. Finalmente, es importante subrayar que incidirá en la inclusión de las personas con discapacidad como parte de la sociedad, a través del acercamiento compartido a la ciencia (Dimensiones de inclusión social y derechos de Schalock; NAVAS MACHO, P. y otros, 2012. Derechos de las personas con discapacidad intelectual: implicaciones de la Convención de Naciones Unidas. Siglo Cero. 43 (243): 7-28.)