Percepción del Personal de Salud sobre la calidad de la atención en adolecentes que abusan ade drogas

En El Salvador los y las adolescentes constituyen la población que más consume drogas, lo que representa un gran desafio para el Ministerio de Salud de El Salvador, entidad responsable de formular politicas que aseguren una atencion de calidad

Competitividad de las MYPES del sector panadería del distrito de Trujillo - 2020.

La investigación tuvo como objetivo determinar el nivel de competitividad en las Micro y Pequeñas Empresas del sector panadería en el Distrito de Trujillo, periodo 2020. Es un estudio con enfoque cuantitativo, de diseño no experimental, trasversal descriptivo. La muestra fue de 30 empresarios panaderos del distrito de Trujillo, La Libertad; se aplicaron dos cuestionarios para la recopilación de los datos que nos permita determinar con objetividad la información necesaria, para la cual se realizó este procesó mediante el software estadístico para ciencias administrativas Excel. Se determinó que existe un nivel alto de competitividad referido a diferenciación en las mypes del sector panadería en el distrito de Trujillo debido a que el 88% de los empresarios panaderos, les dan un valor agregado a sus productos, capacitan a su recurso humano en servicio al cliente, promueven la innovación y diversificación sus productos en sabor y calidad. Finalmente se concluye que el nivel de competitividad de las MYPES del sector panadero del distrito de Trujillo es “alto” debido a que realizan un buen liderazgo en costos, diferenciación y enfoque, utilizando con eficiencia sus recursos, además de contar con la capacidad de innovar y mejorar teniendo en cuenta sus retos y estrategias

Mesenchymal Stromal Cells for Articular Cartilage Repair: Preclinical Studies

[Abstract] Rheumatic diseases such as osteoarthritis (OA) are a major social and economic burden because of the population aging and the lack of curative solutions. An effective cell therapy may be the best treatment option for OA and other cartilage diseases. However, the main cellular strategy used to repair articular cartilage, the transplantation of autologous chondrocytes, is limited to a small number of patients with traumatic lesions. The use of joint replacement after years of disease progression proves the great medical need in current practice. Mesenchymal stromal/stem cells (MSCs) provide an alternative cell source for cartilage regeneration due to numerous advantages, comprising relative ease to isolate and culture, chondrogenic capacity, and antiinflammatory effects. Initial clinical trials with MSCs have led to encouraging results, but many variables have to be considered to attain true amelioration of disease or repair (type and status of cartilage disease, source and conditions of cells, administration regime, combinatorial approaches). Particularly, allogeneic MSCs are an advantageous cellular product. The animal models chosen for preclinical evaluation are also relevant for successful translation into clinical practice. Considering the limitations in the field, rigorous comparative and validating studies in well-established animal models (including large animals) are still needed to set up the bases for additional clinical trials. The present review of studies performed in small and large animal models should help clarify the applicability of MSC-based therapies for articular cartilage repair

Mesenchymal stromal cells for articular cartilage repair: preclinical studies

Rheumatic diseases such as osteoarthritis (OA) are a major social and economic burden because of the population aging and the lack of curative solutions. An effective cell therapy may be the best treatment option for OA and other cartilage diseases. However, the main cellular strategy used to repair articular cartilage, the transplantation of autologous chondrocytes, is limited to a small number of patients with traumatic lesions. The use of joint replacement after years of disease progression proves the great medical need in current practice. Mesenchymal stromal/stem cells (MSCs) provide an alternative cell source for cartilage regeneration due to numerous advantages, comprising relative ease to isolate and culture, chondrogenic capacity, and anti-inflammatory effects. Initial clinical trials with MSCs have led to encouraging results, but many variables have to be considered to attain true amelioration of disease or repair (type and status of cartilage disease, source and conditions of cells, administration regime, combinatorial approaches). Particularly, allogeneic MSCs are an advantageous cellular product. The animal models chosen for preclinical evaluation are also relevant for successful translation into clinical practice. Considering the limitations in the field, rigorous comparative and validating studies in well-established animal models (including large animals) are still needed to set up the bases for additional clinical trials. The present review of studies performed in small and large animal models should help clarify the applicability of MSC-based therapies for articular cartilage repair

Matrícula y titulación de educación superior en El Salvador, con perspectiva de género

El articulo analiza la matrícula y la titulación de la educación superior en El Salvador desde la óptica de perspectiva de género, examinando la tendencia en el quinquenio 2010 – 2014 visualizando que la matrícula a este nivel presenta una equidad por otra parte la titulación de pregrado presenta una feminización, además se hace un recorrido sobre los datos que presentan las instituciones de educación superior al Ministerio de educación sobre la matrícula y titulación de posgrado, dichos datos muestran una equidad del género femenino. Por último se plantea el analices sobre la planta docente universitario por sexo y su titulación de posgrado, donde los datos se inclinan a favor del género masculino

Comparative analysis of different methods for protein quantification in donated human milk

Background: Human milk is the best option for feeding newborns, especially premature infants. In the absence of breast milk, milk from a human milk bank can be a suitable alternative. However, the nutritional content of human milk may be insufficient to meet these high requirements and milk fortification is needed. To facilitate the implementation of simpler and faster analyzers in neonatal healthcare facilities, this study focuses on the concordance analysis of two different analyzers, one based on mid-infrared and the other on ultrasound, in comparison to the Bradford method for determining protein concentration in human milk. Methods: Mature milk samples from donor mothers were collected and pasteurized at the Human Milk Bank of Barcelona and protein quantification was performed using mid-infrared (MIRIS-HMA), ultrasound (MilkoScope Julie27), and the classical Bradford reference methods. The intraclass correlation coefficient (ICC) with 95% confidence interval and Bland-Altman plots were used to assess the agreement between methods. Results: The mean protein concentration of 142 milk samples calculated using MIRIS-HMA, MilkoScope, and the Bradford assay were 1.38, 1.15, and 1.19 g/100 ml, respectively. The ICC was 0.70 for MIRIS-HMA vs. Bradford and 0.37 for MilkoScope vs. Bradford. Conclusion: MIRIS-HMA obtained a better agreement with the Bradford technique and is a promising method for developing new devices based on MIR transmission spectroscopy principles. This study confirms how MIRIS-HMA can be used to accurately calculate the protein concentration of human milk

Investigaciones 2016 acerca del dominio afectivo en matemática educativa

El objetivo de este grupo temático es continuar con el debate comenzado en EIME 2013 (Martínez-Sierra, García, Lemus, Rivera, & Juárez, 2013), EIME 2014 (Martínez-Sierra et al., 2014) y EIME 2015 (Martínez-Sierra et al., 2015) sobre la necesidad y la pertinencia de impulsar en México la investigación sobre el dominio afectivo en matemática educativa. Para alcanzar este objetivo en las sesiones del grupo temático se presentarán los más recientes avances internacionales de investigación en el campo del dominio afectivo. Además, los proponentes de este grupo temático mostrarán resultados de sus propias investigaciones realizadas en diversos aspectos del dominio afectivo de estudiantes y profesores de matemáticas: emociones, actitudes, motivación, creencias, concepciones e identidades matemáticas

Survivin inhibition with YM155 ameliorates experimental pulmonary arterial hypertension

Background: Imbalance between cell proliferation and apoptosis underlies the development of pulmonary arterial hypertension (PAH). Current vasodilator treatment of PAH does not target the uncontrolled proliferative process in pulmonary arteries. Proteins involved in the apoptosis pathway may play a role in PAH and their inhibition might represent a potential therapeutic target. Survivin is a member of the apoptosis inhibitor protein family involved in cell proliferation.Objectives: This study aimed to explore the potential role of survivin in the pathogenesis of PAH and the effects of its inhibition.Methods: In SU5416/hypoxia-induced PAH mice we assessed the expression of survivin by immunohistochemistry, western-blot analysis, and RT-PCR; the expression of proliferation-related genes (Bcl2 and Mki67); and the effects of the survivin inhibitor YM155. In explanted lungs from patients with PAH we assessed the expression of survivin, BCL2 and MKI67.Results: SU5416/hypoxia mice showed increased expression of survivin in pulmonary arteries and lung tissue extract, and upregulation of survivin, Bcl2 and Mki67 genes. Treatment with YM155 reduced right ventricle (RV) systolic pressure, RV thickness, pulmonary vascular remodeling, and the expression of survivin, Bcl2, and Mki67 to values similar to those in control animals. Lungs of patients with PAH also showed increased expression of survivin in pulmonary arteries and lung extract, and also that of BCL2 and MKI67 genes, compared with control lungs.Conclusion: We conclude that survivin might be involved in the pathogenesis of PAH and that its inhibition with YM155 might represent a novel therapeutic approach that warrants further evaluation

Lack of the Glc7 phosphatase regulatory subunit Ypi1 activates the morphogenetic checkpoint

23 páginas, 8 figuras, 1 tablaYpi1 is an essential regulator of the Saccharomyces cerevisiae Glc7 protein phosphatase. Although lack of Ypi1 results in a dramatic blockage in the G2/M cell cycle transition, with abnormally shaped large buds and short spindles, the molecular bases for this phenotype are still obscure. We report here that depletion of Ypi1 results in stabilization of the Pds1 securin, suggesting the activation of a G2/M checkpoint. Depletion of Ypi1 in cells deleted for MAD1/MAD2 or RAD9 still resulted in G2/M blockage, in spite that these cells lack key components of the spindle assembly and DNA damage checkpoints signaling, respectively. In contrast, deletion of SWE1, which encodes a protein kinase required for the morphogenesis checkpoint signaling, allowed passage through G2/M and recovery of normal cell morphology, although the cells did not proliferate. Depletion of Ypi1 caused stabilization of the Swe1 kinase, persistent phosphorylation of protein kinase Cdc28 at Y19, a landmark for morphogenesis checkpoint activation, and depletion of the Cdc11 septin, which explain the failure to form properly assembled septin rings at the bud necks. Deletion of SWE1 restored normal Cdc11 levels in the absence of Ypi1. These results demonstrate that Ypi1 plays an important role in the morphogenesis checkpoint, possibly by regulating Swe1Supported by grants BFU2011-30197-C3-01 to JA, BFU2011-27568 to EQ and BFU2009-11593 to AC (MICINN, Spain). J.A. is recipient of a grant 2009SGR-1091 and an ICREA Academia Award (Generalitat de Catalunya).Peer reviewe