diabetes in active tuberculosis in low income countries to test or to take care

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Treatment of saline produced water through photocatalysis using rGO-TiO 2 nanocomposites

Graphene like-TiO2 nanocomposites (rGO-TiO2) are prepared via hydrothermal route by following different synthetic protocols. The as-prepared nanostructured materials exhibit higher photocatalytic activity than bare TiO2 in the treatment of synthetic produced water containing high salinity levels and different compositions of recalcitrant dissolved organic matter. The effect of the preparation method on the physico-chemical properties is assessed by performing a wide characterization combining different analyses, such as nitrogen physic-adsorption (BET), X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), UV-VIS Diffuse Reflectance Spectroscopy (DRUV) and Electron Paramagnetic Resonance (EPR). The effect of several operative variables (i.e., TiO2/rGO weight ratios, and addition of hydrogen peroxide) on the photocatalytic activity is also critically evaluated. The highest photocatalytic activity is obtained for a rGO/TiO2 weight ratio of about 10%, for which a good compromise between uniformity of dispersion of the TiO2 particles on the rGO layers and covering degree of the titania photoactive surface is achieved. This study can contribute to open new perspectives in the design of high performance graphene like-based TiO2 photocatalysts for removing hydrophobic bio-recalcitrant pollutants from saline water.Peer ReviewedPostprint (author's final draft

Coronavirus Diseases (COVID-19) Current Status and Future Perspectives: A Narrative Review

At the end of 2019 a novel virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causing severe acute respiratory syndrome expanded globally from Wuhan, China. In March 2020 the World Health Organization declared the SARS-Cov-2 virus a global pandemic. We performed a narrative review to describe existing literature with regard to Corona Virus Disease 2019 (COVID-19) epidemiology, pathophysiology, diagnosis, management and future perspective. MEDLINE, EMBASE and Scopus databases were searched for relevant articles. Although only when the pandemic ends it will be possible to assess the full health, social and economic impact of this global disaster, this review represents a picture of the current state of the art. In particular, we focus on public health impact, pathophysiology and clinical manifestations, diagnosis, case management, emergency response and preparedness

Synergism between bisphenol a exposure and overweight/obesity in increasing the malignancy risk in a cohort of patients with thyroid nodules

BPA exposure conferred higher risk of thyroid cancer only in case of concomitant overweight/obesity, therefore suggesting a synergistic action between BPA and the excess of adipose tissue in promoting thyroid carcinogenesis

Radioiodide induces apoptosis in human thyroid tissue in culture.

Radioiodide (131I) is routinely used for the treatment of toxic adenoma, Graves' disease, and for ablation of thyroid remnant after thyroidectomy in patients with thyroid cancer. The toxic effects of ionizing radiations on living cells can be mediated by a necrotic and/or apoptotic process. The involvement of apoptosis in radiation-induced cell death in the thyrocytes has been questioned. The knowledge of the mechanisms that underlie the thyrocyte death in response to radiations can help to achieve a successful treatment with the lowest 131I dose. We developed a method to study the effects of 131I in human thyroid tissue in culture, by which we demonstrated that 131I induces thyroid cell apoptosis. Human thyroid tissues of about 1 mm3 were cultured in vitro and cell viability was determined up to 3 weeks by the MTT assay. Radioiodide added to the culture medium was actively taken up by the tissues. The occurrence of apoptosis in the thyrocytes was assessed by measuring the production of a caspase-cleavage fragment of cytokeratin 18 (M30) by an enzyme-linked immunoassay. Neither variation of cell number nor spontaneous apoptosis was revealed after 1 week of culture. 131I added to the culture medium induced a dose-dependent and a time-dependent generation of M30 fragment. The apoptotic process was confirmed by the generation of caspase-3 and PARP cleavage products. These results demonstrate that 131I induces apoptosis in human thyrocytes. Human thyroid tissue cultures may be useful to investigate the cell death pathways induced by 131I

Histomorphological and functional contralateral symmetry in the gastrocnemius muscles of the laboratory rat

It is usual in anatomical and physiological research to assess the effects of some intervention on extremities (e.g., training programmes or injury recovery protocols) using one muscle for the intervention and its contralateral as control. However, the existence of laterality (left-handedness or right-handedness) in athletes of different specialties is widely recognized. In rats, gastrocnemius is one of the muscles most widely used because of its importance in locomotion and high relative limb mass. Since we have not found studies reporting laterality assessment on the morphology and function in rat gastrocnemius, our study aimed to evaluate the fibre histochemical, morphometrical and muscle force contractile properties between right and left gastrocnemius of the laboratory rat. Fibre type proportion, fibre morphometrical measurements, muscle capillarization and muscle force properties were analysed in the right and left gastrocnemius of six male rats. No statistically significant differences (p=0.265) were found in gastrocnemius to body weight ratio (¿) between right (6.55±0.40) and left (6.49±0.40) muscles. The muscles analysed showed a great degree of heterogeneity in fibre type distribution, having three clearly distinguished regions named red, mixed and white. In the three regions, there were no statistical differences in fibre type proportions between right and left gastrocnemius, as is indicated by the p-values (from 0.203 to 0.941) obtained after running t-Student paired tests for each fibre type. When analysing fibre cross-sectional area, individual fibre capillarization and fibre circularity, no significant differences between right and left gastrocnemius in any of these morphometrical parameters were found in any muscle region or fibre type. Most of the p-values (70%) resulting from running t-Student paired tests were higher than 0.400, and the lowest p-value was 0.115. Seemingly, global capillary and fibre densities were not statistically different between right and left sides in all muscle regions with p-values ranging from 0.337 to 0.812. Force parameters normalized to gastrocnemius mass (mN·g-1) did not show any significant difference between right (PF=74.0±13.4, TF=219.4±13.0) and left (PF=70.9±10.7, TF=213.0±18.0) muscles with p=0.623 (PF) and p=0.514 (TF). Twitch time parameters (ms) also lacked significant differences between the two sides (CT: 43.4±8.6 vs 45.0±14.3, P=0.639; HRT: 77.6±15.0 vs 82.3±25.3, p=0.475). Finally, both muscles also showed similar (p=0.718) fatigue properties. We did find an absence of laterality at the morphological and functional levels, which raises the possibility of using right and left gastrocnemius muscles interchangeably for experimental designs where one muscle is used to analyse data after a physiological intervention and its contralateral muscle plays the control role, thus allowing unbiased paired comparisons to derive accurate conclusions

Genistein and 17β-Estradiol Protect Hepatocytes from Fatty Degeneration by Mechanisms Involving Mitochondria, Inflammasome and Kinases Activation

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