His Last Thoughts Were of You

https://digitalcommons.library.umaine.edu/mmb-vp/4783/thumbnail.jp

Little Alabama Coon / music by Hattie Starr; words by Hattie Starr

https://egrove.olemiss.edu/sharris_d/1001/thumbnail.jp

Paper Doll / words by Johnny S. Black

Cover: drawing of a paper doll; photo of the Mills Bros., an African American vocal group; Publisher: Edward B. Marks Music Co. (New York)https://egrove.olemiss.edu/sharris_e/1066/thumbnail.jp

There\u27ll be Some Changes Made / music by W. Benton Overstreet; words by Billy Higgins

Cover: Genuine Struttin-Blues Series; Publisher: Edward B. Marks Music Co. (New York)https://egrove.olemiss.edu/sharris_d/1051/thumbnail.jp

There\u27ll Be Some Changes Made / music by W. Benton Overstreet; words by Billy Higgins

Cover: Genuine Struttin-Blues series by natural born writers of blues, Listing of songs; Publisher: Edward B. Marks Music Co. (New York)https://egrove.olemiss.edu/sharris_d/1054/thumbnail.jp

Marta, Rambling Rose of the Wildwood / music by Moises Simons; words by L. Wolfe Gilbert

Cover: photo of a man (Arthur Tracy?); Publisher: Edward B. Marks Music Co. (New York)https://egrove.olemiss.edu/sharris_e/1022/thumbnail.jp

A novel assay for monitoring internalization of nanocarrier coupled antibodies

BACKGROUND: Discovery of tumor-selective antibodies or antibody fragments is a promising approach for delivering therapeutic agents to antigen over-expressing cancers. Therefore it is important to develop methods for the identification of target- and function specific antibodies for effective drug delivery. Here we describe a highly selective and sensitive method for characterizing the internalizing potential of multivalently displayed antibodies or ligands conjugated to liposomes into tumor cells. The assay requires minute amounts of histidine-tagged ligand and relies on the non-covalent coupling of these antibodies to fluorescent liposomes containing a metal ion-chelating lipid. Following incubation of cells with antibody-conjugated liposomes, surface bound liposomes are gently removed and the remaining internalized liposomes are quantitated based on fluorescence in a high throughput manner. We have termed this methodology "Chelated Ligand Internalization Assay", or CLIA. RESULTS: The specificity of the assay was demonstrated with different antibodies to the ErbB-2 and EGF receptors. Antibody-uptake correlated with receptor expression levels in tumor cell lines with a range of receptor expression. Furthermore, Ni-NTA liposomes containing doxorubicin were used to screen for the ability of antibodies to confer target-specific cytotoxicity. Using an anti-ErbB2 single chain Fv (scFv) (F5) antibody, cytotoxicity could be conferred to ErbB2-overexpressing cells; however, a poly(ethylene glycol)-linked lipid (DSPE-PEG-NTA-Ni) was necessary to allow for efficient loading of the drug and to reduce nonspecific drug leakage during the course of the assay. CONCLUSION: The CLIA method we describe here represents a rapid, sensitive and robust assay for the identification and characterization of tumor-specific antibodies capable of high drug-delivery efficiency when conjugated to liposomal nanocarriers

That\u27s a Lot of Bunk / words by Al Wilson, James A. Brennan, and Mack Henshaw

Cover: photo of James Barton, a Caucasian male in blackface; cartoon drawings satirizing ideas such as taxi drivers never cheat or landlords never raise the rent; Publisher: Edward B. Marks Music Co. (New York)https://egrove.olemiss.edu/sharris_d/1050/thumbnail.jp