Senate called to order at 11:11 a.m. President Krolicki presiding.

Let us pray. Almighty God, Benevolent Creator, in Your loving plan, You created the world and all in it. With your care, You protect and guide all life in its journey through history and to its fullness. We graciously pray that those who have the responsibility in governing and protecting with just and prudent laws, foster a sense of well-being among the people in Nevada. May this Body legislate with sensitivity for the welfare and true needs of our people. Always influenced by a keen thirst for justice and rightness, may they appreciate the dignity and uniqueness of every person. May they have confidence in what is good and fitting, an eagerness and ability to work together in harmony even through honest disagreement. Strengthened by their collaboration and bonded by their camaraderie, may they support one another in the arduous and challenging moments of decision. Through Your gift of love, may they gain personal peace in their lives and joy in their tasks. We ask this in His Name. AMEN. Pledge of Allegiance to the Flag led by Bishop Randolph R. Calvo, Roma

Ly49H signaling through DAP10 is essential for optimal natural killer cell responses to mouse cytomegalovirus infection

The activating natural killer (NK) cell receptor Ly49H recognizes the mouse cytomegalovirus (MCMV) m157 glycoprotein expressed on the surface of infected cells and is required for protection against MCMV. Although Ly49H has previously been shown to signal via DAP12, we now show that Ly49H must also associate with and signal via DAP10 for optimal function. In the absence of DAP12, DAP10 enables Ly49H-mediated killing of m157-bearing target cells, proliferation in response to MCMV infection, and partial protection against MCMV. DAP10-deficient Ly49H+ NK cells, expressing only Ly49H–DAP12 receptor complexes, are partially impaired in their ability to proliferate during MCMV infection, display diminished ERK1/2 activation, produce less IFN-γ upon Ly49H engagement, and demonstrate reduced control of MCMV infection. Deletion of both DAP10 and DAP12 completely abrogates Ly49H surface expression and control of MCMV infection. Thus, optimal NK cell–mediated immunity to MCMV depends on Ly49H signaling through both DAP10 and DAP12

Innate immunity defines the capacity of antiviral T cells to limit persistent infection

Effective immunity requires the coordinated activation of innate and adaptive immune responses. Natural killer (NK) cells are central innate immune effectors, but can also affect the generation of acquired immune responses to viruses and malignancies. How NK cells influence the efficacy of adaptive immunity, however, is poorly understood. Here, we show that NK cells negatively regulate the duration and effectiveness of virus-specific CD4+ and CD8+ T cell responses by limiting exposure of T cells to infected antigen-presenting cells. This impacts the quality of T cell responses and the ability to limit viral persistence. Our studies provide unexpected insights into novel interplays between innate and adaptive immune effectors, and define the critical requirements for efficient control of viral persistence

Assembly called to order at 11 a.m. Mr. Speaker presiding.

Our God, in the midst of great activity today, we want to take some time to recognize the contribution of those who are our care takers—our nurses—how helpful they are using their skills for the betterment of our health. May their tribe increase. Then we ask You to remind us often of Your invisible presence: That out of confused issues may come simplicity of plan. Out of fear may come confidence, Out of hurry may come the willingness to wait. Out of frustration, rest and power. This we ask in Your name. AMEN. Pledge of allegiance to the Flag. Assemblyman Conklin moved that further reading of the Journal be dispensed with, and the Speaker and Chief Clerk be authorized to make the necessary corrections and additions. Motion carried. REPORTS OF COMMITTEES Mr. Speaker: Your Committee on Health and Human Services, to which was referred Assembly Bill No. 50, has had the same under consideration, and begs leave to report the same back with the recommendation: Do pass. APRIL MASTROLUCA, Chair Mr. Speaker: Your Committee on Judiciary, to which was referred Assembly Bill No. 134, has had th

NOTICE OF EXEMPTION

Almighty God, we pause in this time to thank You for Your love and care for this great State of Nevada. All around us, we see the wonder of Your creation. Guide this Chamber and its Committees as they discuss and propose legislation to this Chamber, and to the Legislature as a whole, to make Nevada even stronger and better for all its residents. May the work done here not be for the good of an individual Senator or political party. We have been called upon by all citizens to work together and guide this State forward. Help us fulfill this awesome responsibility. All these things we bring to You trusting in Your grace and mercy, in the name and power o