Responses of resident (DNA) and active (RNA) microbial communities in fluvial biofilms under different polluted scenarios.

Abstract Pollution from human activities is a major threat to the ecological integrity of fluvial ecosystems. Microbial communities are the most abundant organisms in biofilms, and are key indicators of various pollutants. We investigated the effects some human stressors (nutrients and heavy metals) have on the structure and activity of microbial communities in seven sampling sites located in the Ter River basin (NE Spain). Water and biofilm samples were collected in order to characterize physicochemical and biofilm parameters. The 16S rRNA gene was analysed out from DNA and RNA extracts to obtain α and β diversity. Principal coordinates analyses (PCoA) of the operational taxonomic units (OTUs) in the resident microbial community revealed that nutrients and conductivity were the main driving forces behind the diversity and composition. The effects of mining have had mainly seen on the taxonomic composition of the active microbial community, but also at the OTUs level. Remarkably, metal-impacted communities were very active, which would indicate a close link with the stress faced, that is probably related to the stimulation of detoxification

Una propuesta de evaluación formativa en asignaturas del área de Bases de Datos de la UNNOBA

La evaluación ha sido motivo de reflexión de diversos autores a través del tiempo. La misma está atravesada por la condición humana, el aprendizaje y la construcción del conocimiento, y forma parte del proceso de enseñanza y aprendizaje. Por tal razón, puede ser pensada en un marco multidimensional, que abarca las distintas funciones, momentos y objetos de la acción evaluadora, organizados en un conjunto de ordenadores que responden a las preguntas: ¿qué, por qué, para qué y cómo se evalúa? En este artículo se presenta una propuesta de evaluación formativa por medio del aprendizaje basado en problemas y rúbricas analíticas, mediada por tecnología, en las asignaturas de Bases de Datos 0 (BDO) e Introducción a la Bases de Datos (IBD) de la Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA), siendo su principal motor la retroalimentación formativa.Red de Universidades con Carreras en Informátic

Clinical and radiological characteristics and outcome of wake-up intracerebral hemorrhage

There is little information on the characteristics of patients with wake-up intracerebral hemorrhage (WU-ICH). We aimed to evaluate frequency and relevant differences between WU-ICH and while-awake (WA) ICH patients. This is a retrospective study of a prospective database of consecutive patients with spontaneous ICH, who were classified as WU-ICH, WA-ICH or UO-ICH (unclear onset). We collected demographic, clinical and radiological data, prognostic and therapeutic variables, and outcome [(neurological deterioration, mortality, functional outcome (favorable when modified Rankin scale score 0-2)]. From a total of 466 patients, 98 (25.8%) were classified as UO-ICH according to the type of onset and therefore excluded. We studied 368 patients (mean age 73.9 ± 13.8, 51.4% men), and compared 95 (25.8%) WU-ICH with 273 (74.2%) WA-ICH. Patients from the WU-ICH group were significantly older than WA-ICH (76.9 ± 14.3 vs 72.8 ± 13.6, p = 0.01) but the vascular risk factors were similar. Compared to the WA-ICH group, patients from the WU-ICH group had a lower GCS score or a higher NIHSS score and a higher ICH score, and were less often admitted to a stroke unit or intensive care unit. There were no differences between groups in location, volume, rate of hematoma growth, frequency of intraventricular hemorrhage and outcome. One in five patients with spontaneous ICH are WU-ICH patients. Other than age, there are no relevant differences between WU and WA groups. Although WU-ICH is associated with worse prognostic markers vital and functional outcome is similar to WA-ICH patients

Automated scoring of collaterals, blood pressure, and clinical outcome after endovascular treatment in patients with acute ischemic stroke and large-vessel occlusion

Altres ajuts: Ministerio de Ciencia e Innovación; Fondo Europeo de Desarrollo Regional (FEDER).Introduction: We aimed to determine whether the degree of collateral circulation is associated with blood pressure at admission in acute ischemic stroke patients treated with endovascular treatment and to determine its prognostic value. Methods: We evaluated patients with anterior large vessel occlusion treated with endovascular treatment in a single-center prospective registry. We collected clinical and radiological data. Automated and validated software (Brainomix Ltd., Oxford, UK) was used to generate the collateral score (CS) from the baseline single-phase CT angiography: 0, filling of ≤10% of the occluded MCA territory; 1, 11-50%; 2, 51-90%; 3, >90%. When dichotomized, we considered that CS was good (CS = 2-3), or poor (CS = 0-1). We performed bivariate and multivariable ordinal logistic regression analysis to predict CS categories in our population. The secondary outcome was to determine the influence of automated CS on functional outcome at 3 months. We defined favorable functional outcomes as mRS 0-2 at 3 months. Results: We included 101 patients with a mean age of 72.1 ± 13.1 years and 57 (56.4%) of them were women. We classified patients into 4 groups according to the CS: 7 patients (6.9%) as CS = 0, 15 (14.9%) as CS = 1, 43 (42.6%) as CS = 2 and 36 (35.6%) as CS = 3. Admission systolic blood pressure [aOR per 10 mmHg increase 0.79 (95% CI 0.68-0.92)] and higher baseline NIHSS [aOR 0.90 (95% CI, 0.84-0.96)] were associated with a worse CS. The OR of improving 1 point on the 3-month mRS was 1.63 (95% CI, 1.10-2.44) favoring a better CS (p = 0.016). Conclusion: In acute ischemic stroke patients with anterior large vessel occlusion treated with endovascular treatment, admission systolic blood pressure was inversely associated with the automated scoring of CS on baseline CT angiography. Moreover, a good CS was associated with a favorable outcome

Evaluación de técnicas de data mining para la obtención de perfiles de ingresantes a la UNNOBA

El ingreso a la Universidad es siempre un desafío para el aspirante como para la institución que lo albergará. Durante la inscripción a las propuestas académicas de grado y posterior desarrollo del curso de ingreso la Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA) recolecta información a través de sus sistemas de gestión. Esta información representa un importante activo para la Universidad, en la medida en que pueda ser transformada en conocimiento ya sea para la toma de decisiones que conciernen a la población estudiantil, como para el diseño de planes de acción. La presente línea de investigación propone procesar los datos recolectados por los sistemas de gestión de la UNNOBA durante el ingreso, para obtener en principios patrones o semejanzas entre los aspirantes con idénticos resultados en el curso de ingreso. Esto permitirá entre otras cosas determinar estrategias de aprendizajes adaptativos.Eje: Bases de Datos y Minería de DatosRed de Universidades con Carreras en Informática (RedUNCI

Evaluación de técnicas de data mining para la obtención de perfiles de ingresantes a la UNNOBA

El ingreso a la Universidad es siempre un desafío para el aspirante como para la institución que lo albergará. Durante la inscripción a las propuestas académicas de grado y posterior desarrollo del curso de ingreso la Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA) recolecta información a través de sus sistemas de gestión. Esta información representa un importante activo para la Universidad, en la medida en que pueda ser transformada en conocimiento ya sea para la toma de decisiones que conciernen a la población estudiantil, como para el diseño de planes de acción. La presente línea de investigación propone procesar los datos recolectados por los sistemas de gestión de la UNNOBA durante el ingreso, para obtener en principios patrones o semejanzas entre los aspirantes con idénticos resultados en el curso de ingreso. Esto permitirá entre otras cosas determinar estrategias de aprendizajes adaptativos.Eje: Bases de Datos y Minería de DatosRed de Universidades con Carreras en Informática (RedUNCI

Evaluación de técnicas de data mining para la obtención de perfiles de ingresantes a la UNNOBA

El ingreso a la Universidad es siempre un desafío para el aspirante como para la institución que lo albergará. Durante la inscripción a las propuestas académicas de grado y posterior desarrollo del curso de ingreso la Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA) recolecta información a través de sus sistemas de gestión. Esta información representa un importante activo para la Universidad, en la medida en que pueda ser transformada en conocimiento ya sea para la toma de decisiones que conciernen a la población estudiantil, como para el diseño de planes de acción. La presente línea de investigación propone procesar los datos recolectados por los sistemas de gestión de la UNNOBA durante el ingreso, para obtener en principios patrones o semejanzas entre los aspirantes con idénticos resultados en el curso de ingreso. Esto permitirá entre otras cosas determinar estrategias de aprendizajes adaptativos.Eje: Bases de Datos y Minería de DatosRed de Universidades con Carreras en Informática (RedUNCI

Antitumor Effects of Ral-GTPases Downregulation in Glioblastoma.

Glioblastoma (GBM) is the most common tumor in the central nervous system in adults. This neoplasia shows a high capacity of growth and spreading to the surrounding brain tissue, hindering its complete surgical resection. Therefore, the finding of new antitumor therapies for GBM treatment is a priority. We have previously described that cyclin D1-CDK4 promotes GBM dissemination through the activation of the small GTPases RalA and RalB. In this paper, we show that RalB GTPase is upregulated in primary GBM cells. We found that the downregulation of Ral GTPases, mainly RalB, prevents the proliferation of primary GBM cells and triggers a senescence-like response. Moreover, downregulation of RalA and RalB reduces the viability of GBM cells growing as tumorspheres, suggesting a possible role of these GTPases in the survival of GBM stem cells. By using mouse subcutaneous xenografts, we have corroborated the role of RalB in GBM growth in vivo. Finally, we have observed that the knockdown of RalB also inhibits cell growth in temozolomide-resistant GBM cells. Overall, our work shows that GBM cells are especially sensitive to Ral-GTPase availability. Therefore, we propose that the inactivation of Ral-GTPases may be a reliable therapeutic approach to prevent GBM progression and recurrence.This work was funded by the Catalan Government—AGAUR (2017 SGR-569), Ministerio de Ciencia e Innovaciön (PID2019-104859GB-I00; RTI2018-094739-B-I00; PID2019-104734RB-I00), and by the Xarxa de Bancs de Tumors de Catalunya sponsored by Pla Director d’Oncologia de Catalunya (XBTC). T Cemeli (FPU13/06590), M.Guasch (FPU17/00229), R. Navaridas (FPU18/04480), and M. Ribes (TALENT-IRBLleida) were supported by a pre-doctoral fellowship from Ministerio de Educación, Cultura y Deportes, and from Diputació de Lleida

Quantitative mineralogical comparison between HPGR and ball mill products of a Sn-Ta ore

The mineralogy and liberation characteristics of the comminuted Penouta leucogranite host of the Sn-Ta ore were determined. Grinding developed by a combination of high-pressure grinding rolls (HPGR) followed by a ball mill (BM) was compared with a single ball mill process. The mineral characteristics of the grinding products were analyzed using a Tescan Integrated Mineralogical Analyzer (TIMA-X) and X-ray powder diffraction (XRD). The ore contains 103 ppm of Ta and is mainly composed of quartz, albite, microcline, muscovite, and kaolinite. Nb, Ta-rich minerals are columbite-(Mn) and tantalite-(Mn), as well as minor microlite and wodginite. The liberation in the product is high in the size fraction of less than 250 m (51–52 wt % for columbite-group minerals (CGM) and 74–80 wt % for cassiterite) and reduced in larger particles (8.8–17 wt % for CGM and 28–37 wt % for cassiterite). The recovery in the ¿250 m fraction was high, while in the larger fraction it is limited, remaining up to 80 ppm in some tailings. The combined use of HPGR and a BM reduces the particle size distribution of the product and, thus, increases the liberation of the ores. Smaller fractions can be treated directly using gravity methods; however, particles of a size greater than +250 m should be ground more.Peer ReviewedPostprint (published version

Recommendations for screening, monitoring, prevention, and prophylaxis of infections in adult and pediatric patients receiving CAR T-cell therapy : a position paper

Chimeric antigen receptor (CAR) T-cell therapy is one of the most promising emerging treatments for B-cell malignancies. Recently, two CAR T-cell products (axicabtagene ciloleucel and tisagenlecleucel) have been approved for patients with aggressive B-cell lymphoma and acute lymphoblastic leukemia; many other CAR-T constructs are in research for both hematological and non-hematological diseases. Most of the patients receiving CAR-T therapy will develop fever at some point after infusion, mainly due to cytokine release syndrome (CRS). The onset of CRS is often indistinguishable from an infection, which makes management of these patients challenging. In addition to the lymphodepleting chemotherapy and CAR T cells, the treatment of complications with corticosteroids and/or tocilizumab increases the risk of infection in these patients. Data regarding incidence, risk factors and prevention of infections in patients receiving CAR-T cell therapy are scarce. To assist in patient care, a multidisciplinary team from hospitals designated by the Spanish Ministry of Health to perform CAR-T therapy prepared these recommendations. We reviewed the literature on the incidence, risk factors, and management of infections in adult and pediatric patients receiving CAR-T cell treatment. Recommendations cover different areas: monitoring and treatment of hypogammaglobulinemia, prevention, prophylaxis, and management of bacterial, viral, and fungal infections as well as vaccination prior and after CAR-T cell therapy