Leading interactions in the β\beta-SrV6O15Sr V_6 O_{15} compound

The present study shows that the electronic structure of the $\beta$-$AV\_6O\_{15}$ family of compounds ($A = Sr, Ca, Na ...$) is based on weakly interacting two-leg ladders, in contrast with the zig-zag chain model one could expect from their crystal structure. Spin dimer analysis, based on extended H\"{u}ckel tight-binding calculations, was performed to determine the structure of the dominant transfer and magnetic interactions in the room temperature $\beta$-$SrV\_6O\_{15}$ phase. Two different two-legs ladders, associated with different charge/spin orders are proposed to describe these one-dimensional $\beta$-type systems. The antiferromagnetic ladders are packed in an 'IPN' geometry and coupled to each other through weak antiferromagnetic interactions. This arrangement of the dominant interactions explains the otherwise surprising similarities of the optical conductivity and Raman spectra for the one-dimensional $\beta$-type phases and the two-dimensional $\alpha$-type ones such as the well-known $\alpha^\prime$-$NaV\_2O\_5$ system

Stress Effects on Psychological Distress among Asian Americans: Structural Equation Modeling Analysis

We examined the relationship between stress and psychological distress as moderated by religious affiliation among Asian Americans. We conducted secondary analysis of data from respondents who self-identified as Chinese, Filipino, or Vietnamese (N = 1,614) from the National Latino and Asian American Study. We used structural equation modeling to investigate relationships between culturally nuanced stress indicators (discrimination, stressful family relationships, and financial difficulties) and psychological distress. The moderation effect of religious affiliation on the relationship between stress and psychological distress was examined using multiple-group analysis. Analyses suggested a statistically significant positive main effect of each stress variable on psychological distress. No support of a moderation effect was found; religious affiliation did not significantly attenuate the impact of stress on psychological distress in this Asian heritage community sample. Our findings highlight the need for further examination of religious involvement rather than religious affiliation as a buffer against daily stress experienced by Asian Americans

A 58-Year-Old Woman with Abdominal Symptoms and Elevated C-Reactive Protein

Babak Javid and colleagues discuss the differential diagnosis, investigation, and management of a woman with abdominal symptoms and a raised C-reactive protein

Detection of a close supernova gravitational wave burst in a network of interferometers, neutrino and optical detectors

Trying to detect the gravitational wave (GW) signal emitted by a type II supernova is a main challenge for the GW community. Indeed, the corresponding waveform is not accurately modeled as the supernova physics is very complex; in addition, all the existing numerical simulations agree on the weakness of the GW emission, thus restraining the number of sources potentially detectable. Consequently, triggering the GW signal with a confidence level high enough to conclude directly to a detection is very difficult, even with the use of a network of interferometric detectors. On the other hand, one can hope to take benefit from the neutrino and optical emissions associated to the supernova explosion, in order to discover and study GW radiation in an event already detected independently. This article aims at presenting some realistic scenarios for the search of the supernova GW bursts, based on the present knowledge of the emitted signals and on the results of network data analysis simulations. Both the direct search and the confirmation of the supernova event are considered. In addition, some physical studies following the discovery of a supernova GW emission are also mentioned: from the absolute neutrino mass to the supernova physics or the black hole signature, the potential spectrum of discoveries is wide.Comment: Revised version, accepted for publication in Astroparticle Physic

A novel method for assessing the renal biopsy specimens using an activatable fluorescent probe

Gamma-glutamyl hydroxymethyl rhodamine green (gGlu-HMRG) is an activatable fluorescent probe that can be activated by γ-glutamyltranspeptidase (GGT). The expression of GGT in the kidney, which is one of the major organs exhibiting enhanced GGT expression, is exclusively localised to the cortex. Here, we aimed to investigate the feasibility of gGlu-HMRG as a probe for the on-site assessment of renal biopsy specimens. gGlu-HMRG fluorescent probe was applied to the renal proximal tubular epithelial cells and cortical collecting duct cells in vitro, mouse kidneys ex vivo, and human biopsy specimens. In addition, the fluorescence intensities in the cortex and the medulla were comparatively evaluated in the biopsy specimens. The fluorescence signal was rapidly detected in the renal proximal tubular epithelial cells, whereas that in the cortical collecting duct cells was not detected. The fluorescence signal was detected in the mouse kidneys ex vivo without markedly affecting the tissue morphology. In the human biopsy specimens, the fluorescence signal in the cortex was significantly distinct from that in the medulla (p?<?0.05). Thus, this fluorescent probe can be used to distinctly identify the renal cortex in the biopsy specimens

ruvA Mutants that resolve Holliday junctions but do not reverse replication forks

RuvAB and RuvABC complexes catalyze branch migration and resolution of Holliday junctions (HJs) respectively. In addition to their action in the last steps of homologous recombination, they process HJs made by replication fork reversal, a reaction which occurs at inactivated replication forks by the annealing of blocked leading and lagging strand ends. RuvAB was recently proposed to bind replication forks and directly catalyze their conversion into HJs. We report here the isolation and characterization of two separation-of-function ruvA mutants that resolve HJs, based on their capacity to promote conjugational recombination and recombinational repair of UV and mitomycin C lesions, but have lost the capacity to reverse forks. In vivo and in vitro evidence indicate that the ruvA mutations affect DNA binding and the stimulation of RuvB helicase activity. This work shows that RuvA's actions at forks and at HJs can be genetically separated, and that RuvA mutants compromised for fork reversal remain fully capable of homologous recombination

Polysaccharides from the South African medicinal plant Artemisia afra: Structure and activity studies

Artemisia afra (Jacq. Ex. Willd), is an indigenous plant in South Africa and other parts of the African continent, where it is used as traditional medicine mostly for respiratory conditions. The objective of this study was to investigate the structural features of the polysaccharides from the leaves of this plant, as well as the biological activities of the polysaccharide fractions against the complement assay. Leaves of Artemisia afra were extracted sequentially with organic solvents (dichloromethane and methanol), 50% aqueous ethanol, and water at 50 and 100 °C respectively. The polysaccharide extracts were fractionated by ion exchange chromatography and the resulting fractions were tested for biological activity against the complement fixation assay. Active fractions were further fractionated using gel filtration. Monosaccharide compositions and linkage analyses were determined for the relevant fractions. Polysaccharides were shown to be of the pectin type, and largely contain arabinogalactan, rhamnogalacturonan and homogalacturonan structural features. The presence of arabinogalactan type II features as suggested by methylation analysis was further confirmed by the ready precipitation of the relevant polysaccharides with the Yariv reagent. An unusual feature of some of these polysaccharides was the presence of relatively high levels of xylose as one of its monosaccharide constituents. Purified polysaccharide fractions were shown to possess higher biological activity than the selected standard in the complement assay. Digestion of these polysaccharides with an endo-polygalacturonase enzyme resulted in polymers with lower molecular weights as expected, but still with biological activity which exceeded that of the standard. Thus on the basis of these studies it may be suggested that immunomodulating properties probably contribute significantly to the health-promoting effects of this medicinal plant

A large scale hearing loss screen reveals an extensive unexplored genetic landscape for auditory dysfunction

The developmental and physiological complexity of the auditory system is likely reflected in the underlying set of genes involved in auditory function. In humans, over 150 non-syndromic loci have been identified, and there are more than 400 human genetic syndromes with a hearing loss component. Over 100 non-syndromic hearing loss genes have been identified in mouse and human, but we remain ignorant of the full extent of the genetic landscape involved in auditory dysfunction. As part of the International Mouse Phenotyping Consortium, we undertook a hearing loss screen in a cohort of 3006 mouse knockout strains. In total, we identify 67 candidate hearing loss genes. We detect known hearing loss genes, but the vast majority, 52, of the candidate genes were novel. Our analysis reveals a large and unexplored genetic landscape involved with auditory function

Human Tears Reveal Insights into Corneal Neovascularization

Corneal neovascularization results from the encroachment of blood vessels from the surrounding conjunctiva onto the normally avascular cornea. The aim of this study is to identify factors in human tears that are involved in development and/or maintenance of corneal neovascularization in humans. This could allow development of diagnostic tools for monitoring corneal neovascularization and combination monoclonal antibody therapies for its treatment. In an observational case-control study we enrolled a total of 12 patients with corneal neovascularization and 10 healthy volunteers. Basal tears along with reflex tears from the inferior fornix, superior fornix and using a corneal bath were collected along with blood serum samples. From all patients, ocular surface photographs were taken. Concentrations of the pro-angiogenic cytokines interleukin (IL)-6, IL-8, Vascular Endothelial Growth Factor (VEGF), Monocyte Chemoattractant Protein 1 (MCP-1) and Fas Ligand (FasL) were determined in blood and tear samples using a flow cytometric multiplex assay. Our results show that the concentration of pro-angiogenic cytokines in human tears are significantly higher compared to their concentrations in serum, with highest levels found in basal tears. Interestingly, we could detect a significantly higher concentration of IL- 6, IL-8 and VEGF in localized corneal tears of patients with neovascularized corneas when compared to the control group. This is the first study of its kind demonstrating a significant difference of defined factors in tears from patients with neovascularized corneas as compared to healthy controls. These results provide the basis for future research using animal models to further substantiate the role of these cytokines in the establishment and maintenance of corneal neovascularization