Characterization and antimicrobial activities of two Streptomyces isolates from soil in the periphery of Universiti Putra Malaysia.

This study was to assess the identification and antimicrobial activities of two actinomycete isolates. The two isolates designated as B8 and C2, were isolated from a patch of soil in the peripheral area of Universiti Putra Malaysia by streaking on starch casein agar after standard serial dilution procedures. Their antimicrobial activities were first evaluated against eight clinical laboratory strains namely Bacillus sp., Enterococcus sp., Escherichia coli, Klebsiella sp., Pseudomonas sp., Salmonella sp., Staphylococcus aureus, and Staphylococcus epidermidis by perpendicular streak method on Mueller Hinton and Tryptic Soy agar. In both media, a broad-spectrum antibacterial activity was observed for both isolates, with B8 against all the test bacteria and C2 against five of them (Bacillus sp., E. coli, Pseudomonas sp., S. aureus and S. epidermidis). Re-assessment against E. coli ATCC 25922 and S. aureus ATCC 25923 strains by similar method showed antibacterial activities by isolate B8 against both ATTC strains while C2 only against S. aureus ATCC 25923. Streptomyces griseus ATCC 10137 was included in the later experiment and showed antibacterial activity against both ATCC strains. Subsequently, the two isolates were identified by PCR/sequencing techniques and phylogenetic analysis to be Streptomyces species (>93% homology based on 16S rRNA and rpoB genes). Characterization on cultural characteristic and viable count at different temperatures (37ºC and 28ºC), on different microbiological media (AIA, ISP-2, MHA, NA, PDA and TSA), were performed. More morphological features were observed on ISP-2 for both isolates. A higher growth yield was also observed at 28ºC in all media but in comparing that between the two isolates, isolate B8 outnumbered C2 at all experimental conditions. The observed variation in cultural traits and growth yield indicate unique properties between the two antibiotic-producing isolates

Users’ preferences on the provision of facilities for mass rapid transit Sungai Buloh – Subang - Putrajaya line (MRT2) stations

National Transport Policy (2019-2030) strives to improve the transportation sector towards a conducive and competitive sector while fulfils the demands of people. The new development of Mass Rapid Transit (MRT2) Sungai Buloh – Subang – Putrajaya Line is expected to complete in 2022 and would benefit 529,000 users. This paper analyses the preferences of users on the facility provision at mass rapid transit stations in the local context. This paper was based on secondary and primary data sources such as official documents, websites and data collected for the Integrated MRT2 Land Use Masterplan. Document analysis method was employed to review official sources from public transport providers across selected benchmarked countries. Six categories of facilities at mass rapid transit stations were evaluated using 40 criteria. Results suggested improvements are needed in barrier-free considerations and convenient facilities. Statistical analyses, involving descriptive and inferential analyses, were conducted. The results show that gender and travel time to station influenced users’ preferences on facilities needed at stations. This paper suggests that certain criteria of facilities must be considered based on universal needs of the population to encourage usage of MRT2 as public transport as well as to provide convenient travel experiences

Assessing the current implementation of transit oriented development concept in Malaysia: Case study of MRT Putrajaya line

In the past years, the concept of Transit-Oriented Development has been adopted in cities and countries including Malaysia and Singapore. The integration of land use and public transport stations through Transit-Oriented Development (TOD) as part of urban and cities strategy is highly acclaimed in promoting sustainable development concept in cities development. To understand the performance of TODs implementation in Klang Valley, this study has selected eleven stations in of Mass Rapid Transit (MRT) Putrajaya Line as case studies. This paper aims to evaluate the current implementation of TODs in Malaysia, benchmarked against the TOD land use composition and percentage from Singapore’s model. The findings show that Raja Uda, Ampang Park and Persiaran KLCC stations show significant performance. However, Bandar Malaysia North station displayed poor results with the lowest percentage of residential and commercial components. All stations did not achieve the ideal TOD value for residential land use and eight out of eleven stations recorded higher than the ideal TOD value for roads. However, highest number of stations achieved the ideal TOD value for mixed-use and commercial land uses. These results would help policymakers to improve the current implementation of TODs in Malaysia

Global Retinoblastoma Presentation and Analysis by National Income Level.

Importance: Early diagnosis of retinoblastoma, the most common intraocular cancer, can save both a child's life and vision. However, anecdotal evidence suggests that many children across the world are diagnosed late. To our knowledge, the clinical presentation of retinoblastoma has never been assessed on a global scale. Objectives: To report the retinoblastoma stage at diagnosis in patients across the world during a single year, to investigate associations between clinical variables and national income level, and to investigate risk factors for advanced disease at diagnosis. Design, Setting, and Participants: A total of 278 retinoblastoma treatment centers were recruited from June 2017 through December 2018 to participate in a cross-sectional analysis of treatment-naive patients with retinoblastoma who were diagnosed in 2017. Main Outcomes and Measures: Age at presentation, proportion of familial history of retinoblastoma, and tumor stage and metastasis. Results: The cohort included 4351 new patients from 153 countries; the median age at diagnosis was 30.5 (interquartile range, 18.3-45.9) months, and 1976 patients (45.4%) were female. Most patients (n = 3685 [84.7%]) were from low- and middle-income countries (LMICs). Globally, the most common indication for referral was leukocoria (n = 2638 [62.8%]), followed by strabismus (n = 429 [10.2%]) and proptosis (n = 309 [7.4%]). Patients from high-income countries (HICs) were diagnosed at a median age of 14.1 months, with 656 of 666 (98.5%) patients having intraocular retinoblastoma and 2 (0.3%) having metastasis. Patients from low-income countries were diagnosed at a median age of 30.5 months, with 256 of 521 (49.1%) having extraocular retinoblastoma and 94 of 498 (18.9%) having metastasis. Lower national income level was associated with older presentation age, higher proportion of locally advanced disease and distant metastasis, and smaller proportion of familial history of retinoblastoma. Advanced disease at diagnosis was more common in LMICs even after adjusting for age (odds ratio for low-income countries vs upper-middle-income countries and HICs, 17.92 [95% CI, 12.94-24.80], and for lower-middle-income countries vs upper-middle-income countries and HICs, 5.74 [95% CI, 4.30-7.68]). Conclusions and Relevance: This study is estimated to have included more than half of all new retinoblastoma cases worldwide in 2017. Children from LMICs, where the main global retinoblastoma burden lies, presented at an older age with more advanced disease and demonstrated a smaller proportion of familial history of retinoblastoma, likely because many do not reach a childbearing age. Given that retinoblastoma is curable, these data are concerning and mandate intervention at national and international levels. Further studies are needed to investigate factors, other than age at presentation, that may be associated with advanced disease in LMICs

The global retinoblastoma outcome study : a prospective, cluster-based analysis of 4064 patients from 149 countries

DATA SHARING : The study data will become available online once all analyses are complete.BACKGROUND : Retinoblastoma is the most common intraocular cancer worldwide. There is some evidence to suggest that major differences exist in treatment outcomes for children with retinoblastoma from different regions, but these differences have not been assessed on a global scale. We aimed to report 3-year outcomes for children with retinoblastoma globally and to investigate factors associated with survival. METHODS : We did a prospective cluster-based analysis of treatment-naive patients with retinoblastoma who were diagnosed between Jan 1, 2017, and Dec 31, 2017, then treated and followed up for 3 years. Patients were recruited from 260 specialised treatment centres worldwide. Data were obtained from participating centres on primary and additional treatments, duration of follow-up, metastasis, eye globe salvage, and survival outcome. We analysed time to death and time to enucleation with Cox regression models. FINDINGS : The cohort included 4064 children from 149 countries. The median age at diagnosis was 23·2 months (IQR 11·0–36·5). Extraocular tumour spread (cT4 of the cTNMH classification) at diagnosis was reported in five (0·8%) of 636 children from high-income countries, 55 (5·4%) of 1027 children from upper-middle-income countries, 342 (19·7%) of 1738 children from lower-middle-income countries, and 196 (42·9%) of 457 children from low-income countries. Enucleation surgery was available for all children and intravenous chemotherapy was available for 4014 (98·8%) of 4064 children. The 3-year survival rate was 99·5% (95% CI 98·8–100·0) for children from high-income countries, 91·2% (89·5–93·0) for children from upper-middle-income countries, 80·3% (78·3–82·3) for children from lower-middle-income countries, and 57·3% (52·1-63·0) for children from low-income countries. On analysis, independent factors for worse survival were residence in low-income countries compared to high-income countries (hazard ratio 16·67; 95% CI 4·76–50·00), cT4 advanced tumour compared to cT1 (8·98; 4·44–18·18), and older age at diagnosis in children up to 3 years (1·38 per year; 1·23–1·56). For children aged 3–7 years, the mortality risk decreased slightly (p=0·0104 for the change in slope). INTERPRETATION : This study, estimated to include approximately half of all new retinoblastoma cases worldwide in 2017, shows profound inequity in survival of children depending on the national income level of their country of residence. In high-income countries, death from retinoblastoma is rare, whereas in low-income countries estimated 3-year survival is just over 50%. Although essential treatments are available in nearly all countries, early diagnosis and treatment in low-income countries are key to improving survival outcomes.The Queen Elizabeth Diamond Jubilee Trust and the Wellcome Trust.https://www.thelancet.com/journals/langlo/homeam2023Paediatrics and Child Healt

Perempuan Menolak Tabu

242 hal;ill

FAKTOR PEMANFAATAN PELAYANAN VCT HIV PADA IBU HAMIL PESERTA ANC DI BEBERAPA PUSKESMAS KOTA MAKASSAR

JURNAL MASYARAKAT EPIDEMIOLOGI INDONESIAABSTRACT\ud \ud Transmission of Human Immunodeficiency Virus (HIV) from mother to baby at this time increased with increasing HIV-infected women. This study aims to analyze the factors associated with the utilization of VCT services in pregnant women in ANC attendees Several Makassar Health Center 2013. The research was conducted in three health centers in the city of Makassar in Jumpandang Baru health center, Kassi-Kassi health center, and Jongaya health center. The study design was a cross sectional study. Sample of 230 respondents, where pdata conducted by interview using a questionnaire. Bivariate statistical tests using Chi square test (??2) at a significance level of p <0.05 and confidence interval (CI) 95%, stratification using Hanszel Mantel test and multivariate analysis using logistic regression. The results showed that factors related to HIV VCT service utilization among pregnant women is a standard implementation of the ANC (p = 0.004, RP1, 54, 95% CI: 0.072 to 0.387), knowledge about HIV, HIV VCT and PMTCT (p = 0.018, RP1, 282, 95% CI: 1.044 to 1.576), and the support of her husband and family (p = 0.002, RP 1.391, 95% CI: 1.137 to 1.701). The factors that are not related to the attitude towards HIV VCT service utilization (p = 0.859, RP 0.981, 95% CI: 0.795 to 1.210), and access to health care (p = 0.752, RP 0.820, 95% CI: 0.204 to 3.292). ANC service standards and support of her husband and family is the most influential variable on HIV VCT service utilization in pregnant women. Suggested increasing knowledge and awareness about the importance of service standards officers ANC, as well as increased knowledge about HIV / AIDS, VCT and PMTCT in pregnant women.\ud \ud Keywords: utilization factor, HIV VCT, AN

A comparative analysis of transit oriented development guidelines between Malaysia and other countries

Transit Oriented Development (TOD) is an emerging concept to optimise the land use development surrounding a transit station and to create a reliable relationship between a densely compact urban form and high public transportation ridership. In Malaysia, the concept of TOD was initiated in the first National Physical Plan in 2005 and mentioned again in National Physical Plan 2 in 2010. This paper identifies the principle of TOD applied in Malaysia and other countries, and discusses the differences and similarities of the TOD practices using comparative analysis and document analysis methods. Findings of this study indicate that mixed-use development, high density, intensity and connectivity are the main TOD principles adopted in Malaysia and other countries around the globe. The current policy and practises of these countries tend to focus more on the better management and increase ridership of these transit rail stations, including ways to encourage users to shift from private vehicles to public transportation. The findings of this study would contribute towards policy decisions and practices of TOD in Malaysia. © 2021 Malaysian Institute Of Planners. All rights reserved

Peranan Orang Tua Dalam Pendidikan Keluarga Menurut Penafsiran Sayyid Quthb Dalam Tafsir Fi Zhialal Al-Qur'an (Kajian Atas Q.S. Attahrim Ayat 6)

170 hlm.: ilus.; 23 c

Documentation relating to the federal government's policy of detente

The federal Government's ostpolitik, its policy towards Eastern Europe, was formally rounded off with the agreement of december 21, 1973, on the establishment of diplomatic relations with bulgaria and hungary.142 p.: 20 c