Studies on Xenopus laevis intestine reveal biological pathways underlying vertebrate gut adaptation from embryo to adult

The developmental transcriptome of the Xenopus laevis intestine, from embryo to adult, reveals insights into the regulation of gut development in all vertebrates

The Seascape of Demersal Fish Nursery Areas in the North Mediterranean Sea, a First Step Towards the Implementation of Spatial Planning for Trawl Fisheries

The identification of nursery grounds and other essential fish habitats of exploited stocks is a key requirement for the development of spatial conservation planning aimed at reducing the adverse impact of fishing on the exploited populations and ecosystems. The reduction in juvenile mortality is particularly relevant in the Mediterranean and is considered as one of the main prerequisites for the future sustainability of trawl fisheries. The distribution of nursery areas of 11 important commercial species of demersal fish and shellfish was analysed in the European Union Mediterranean waters using time series of bottom trawl survey data with the aim of identifying the most persistent recruitment areas. A high interspecific spatial overlap between nursery areas was mainly found along the shelf break of many different sectors of the Northern Mediterranean indicating a high potential for the implementation of conservation measures. Overlap of the nursery grounds with existing spatial fisheries management measures and trawl fisheries restricted areas was also investigated. Spatial analyses revealed considerable variation depending on species and associated habitat/depth preferences with increased protection seen in coastal nurseries and minimal protection seen for deeper nurseries (e.g. Parapenaeus longirostris 6%). This is partly attributed to existing environmental policy instruments (e.g. Habitats Directive and Mediterranean Regulation EC 1967/2006) aiming at minimising impacts on coastal priority habitats such as seagrass, coralligenous and maerl beds. The new knowledge on the distribution and persistence of demersal nurseries provided in this study can support the application of spatial conservation measures, such as the designation of no-take Marine Protected Areas in EU Mediterranean waters and their inclusion in a conservation network. The establishment of no-take zones will be consistent with the objectives of the Common Fisheries Policy applying the ecosystem approach to fisheries management and with the requirements of the Marine Strategy Framework Directive to maintain or achieve seafloor integrity and good environmental status.Versión del editor4,411

Disease presence in goat flocks on temperate valleys of Argentina’s Northwest

Una encuesta transversal ad hoc se llevó a cabo en veintitrés majadas caprinas correspondientes a unidades productivas (UP) familiares extensivos (UPF, n=19) y semiintensivas (UPL, n=4) dedicadas a la producción de leche en los valles templados de Salta y Jujuy con el objetivo de describir la presencia de enfermedades en sus majadas. Se recabaron datos del manejo general y sanitario en particular. Se procesaron sueros para el diagnóstico de brucelosis por las pruebas tamiz del antígeno bufferado en placa y confirmadas por polarización fluorescente. Paraparatuberculosis, herpesvirus caprino tipo 1 (CaHV-1), neosporosis y toxoplasmosis se utilizó ELISA indirecto y para leptospirosis microaglutinación. Se determinaron los valores de Cu2+, Zn2+, Mg2+ y Ca2+ sérico por espectrofotometría de absorción atómica. En heces, se realizó el recuento de huevos de helmintos por gramo (hpg) y la diferenciación de géneros por coprocultivo. Las UP muestreadas crían en promedio 48,4 ±30,7 (UPF) y 221,3 ±78,7 (UPL) caprinos respectivamente, sin contar los cabritos menores a los 45 días de edad. La cría de las UPF fue extensiva con encierre nocturno y la alimentación sobre pastizal natural mientras que las UPL combinaban pasturas bajo riego con suplementación en base de concentrados. Existen dos períodos de pariciones al año; el principal donde pare casi la totalidad de la majada se localiza desde fines de marzo a fines de septiembre y el segundo período donde pare una menor proporción de cabras se ubica desde septiembre a diciembre. El 94,7% de los productores desparasitaba en forma rutinaria toda la majada y el 75% trataban los piojos. En un 41,2% de las UP se inyectaba mineralizantes-vitamínicos a toda la majada y en solo un 15% vacunaba contra enfermedades Clostridiales. El promedio de la tasa de mortalidad anual fue del 20,1±9,8% y 7,8±3,0% respectivamente en UPF y UPL. En el 100% de las UPF se registró la ocurrencia de abortos, y de estos el 25% presentó retención de placenta. El promedio intramajada de abortos comunicados fue del 14,5 ±11,6%. El 58,8% de las UP declaró tener casos de ectima contagioso. Se registraron un 26,7% cuadros de muertes agudas probablemente por infecciones Clostridiales. En el 93,3% de las UPF se registraron problemas de mastitis. El porcentaje de UP con seroprevalencia positiva a brucelosis, leptospirosis, paratuberculosis, toxoplasmosis, neosporosis y CpHV-1 fue respectivamente de 0%, 0%, 0%, 73,3%, 40% y 87,5%. Los promedios de los hpg de invierno – primavera y los de verano-otoño fueron respectivamente de 965 y 2859 hpg, con Haemonchus y Trichostrongylus y en menor medida Teladorsagia como nematodes predominantes. En el 31,6% de las UP se diagnosticó Fasciolosis. El 66,7% de las UP describió la presencia, a la faena, de quistes hidatídicos. En un 58% de las majadas se describió bocio. Los promedios por UP de cobre, zinc, magnesio y calcio séricos fueron respectivamente 0,59±0,10 ppm, 0,67±0,05 ppm, 2,30±0,29mg/dl y 8,42±1,92 mg/dl. Sin embargo, para el cobre y el calcio los valores promedios difieren (p<0,05) entre las UPF (0,66±0,08 ppm y 9,36 ±1,85 mg/dl) y las UPL (0,54±0,06 ppm y 7,02±0,76 mg/dl). Un 63,6% de las UP dijo tener intoxicación con cegadera (Heterophyllaea pustulata) y de muertes (50%) en animales jóvenes atribuidas al romerillo (Baccharis coridifolia) y en menor medida a la hediondilla (Cestrum parqui). Un 58,8% de los propietarios declararon tener problemas de pérdidas de animales principalmente por pumas. Estos primeros resultados muestran la importancia de llevar a cabo estudios más específicos respecto de la salud de las majadas para incrementar la producción y el bienestar general de las familias productoras.A cross-sectional survey was performed in 23 goat flocks (UP) of family extensive farming units (UPF) and semi-intensive farming units (UPL) from the temperate valleys of Jujuy and Salta provinces, with the aim of describing disease occurrence in goat flocks. Farmers were subjected to an ad hoc survey to gather data on general management and specific health issues. Sera were processed to diagnose brucellosis using the BPA screening test and positive sera were confirmed by FPA test. Paratuberculosis, caprine herpesvirus 1, toxoplasmosis and neosporosis were tested by indirect ELISA, and leptospirosis by microagglutination test. Serum Cu2+, Zn2+, Mg2+ and Ca2+ were determined by atomic absorption spectrophotometer. Fecal samples were taken for eggs counts per gram of feaces (epg) and identifying genera by coproculture. UP had on average 48,4 ±30,7 (UPF) y 221,3 ±78,7 (UPL) goats (not including kids). UPF animals are extensively bred, with nocturnal enclosure, and flock feeding is based on grazing the hills and UPL combined pastures under irrigation with concentrate-based supplementation. Kidding is concentrated in two periods: from late March to late September, when almost all the flock kids, and a second minor kid period that was from September to December. Farmers (94,7%) routinely treat all the flock against parasites and the 71,8% treat against lice. The 41,2% of UP provide all the flock with mineral-vitamin mixtures and only the 15, % vaccinated against clostridium diseases. Mean annual mortality rate per flock was 20,1±9,8% and 7,8±3,0% respectively for UPF and UPL. Abortions were recorded in 100% of the UP, 25% with retained placenta. Mean within-flock reported abortions were 14,5 ±11,6%. Contagious echtyma (orf) was detected in 58,8% of the UP and acute deaths probably due to clostridial infections were recorded in 26,7% of the UP. Mastitis-associated problems were recorded in 93,3% of UP. Positive seroprevalence of brucellosis, leptospirosis, paratuberculosis, toxoplasmosis, neosporosis and CpHV-1, in UP was 0%, 25%, 50%, 42,8%, 88,8%, 85,7%, 12,5% and 0% respectively. Mean epg were 965 and 2859 for winter-spring and summer-autumn respectively, with Haemonchus and Trichostrongylus and secondly Teladorsagia being the prevailing nematodes. Fasciola infections were recorded in 31,6% of the UP. The presence of hydatid cysts in slaughtered animals were indicated by 66,7% of UP. Signs of goiter were described in 58% of the flocks. Mean serum cooper, zinc, magnesium and calcium per UP were 0,59±0,10 ppm, 0,67±0,05 ppm, 2,30±0,29mg/dl and 8,42±1,92 mg/dl respectively. However cooper and calcium was different (p<0,05) between UPF (0,66±0,08 ppm and 9,36±1,85 mg/dl) and UPL (0,54±0,06 ppm and 7,02±0,76 mg/dl). A 63,6% of the UP reported cases of intoxication by cegadera (Heterophyllaea pustulata) and death (50%) were reported and attributed by farmers to the ingestion of romerillo (Baccharis coridifolia) and secondly of hediondilla (Cestrum parqui) by young animals. Some farmers (58.8%) indicated loss of animals mostly due to the attack by pumas. These first results regarding show the importance of conducting specific studies about the health of flocks in order to increase competitiveness in goat production and the welfare of farming families.Fil: Suarez, Víctor Humberto. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Investigación Animal del Chaco Semiárido; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Dodero, Ana Mariela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Investigación Animal del Chaco Semiárido; ArgentinaFil: Almudevar, F. M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Bertoni, E. A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Investigación Animal del Chaco Semiárido; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Salatin, A. O.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Investigación Animal del Chaco Semiárido; ArgentinaFil: Viñabal, A. E.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Investigación Animal del Chaco Semiárido; ArgentinaFil: Saldaño, R.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Martínez, G. M.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Investigación Animal del Chaco Semiárido; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; ArgentinaFil: Fiorentino, Maria Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; ArgentinaFil: Brihuega, Bibiana. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar; ArgentinaFil: Romera, Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar; Argentin

Long-term home ventilation of children in Italy: A national survey.

BACKGROUND: Improved technology, as well as professional and parental awareness, enable many ventilator-dependent children to live at home. However, the profile of this growing population, the quality and adequacy of home care, and patients' needs still require thorough assessment. OBJECTIVES: To define the characteristics of Italian children receiving long-term home mechanical ventilation (HMV) in Italy. METHODS: A detailed questionnaire was sent to 302 National Health Service hospitals potentially involved in the care of HVM in children (aged <17 years). Information was collected on patient characteristics, type of ventilation, and home respiratory care. RESULTS: A total of 362 HMV children was identified. The prevalence was 4.2 per 100,000 (95% CI: 3.8-4.6), median age was 8 years (interquartile range 4-14), median age at starting mechanical ventilation was 4 years (1-11), and 56% were male. The most frequent diagnostic categories were neuromuscular disorders (49%), lung and upper respiratory tract diseases (18%), hypoxic (ischemic) encephalopathy (13%), and abnormal ventilation control (12%). Medical professionals with nurses (for 62% of children) and physiotherapists (20%) participated in the patients' discharge from hospital, though parents were the primary care giver, and in 47% of cases, the sole care giver. Invasive ventilation was used in 41% and was significantly related to young age, southern regional residence, longer time spent under mechanical ventilation, neuromuscular disorders, or hypoxic (ischemic) encephalopathy. CONCLUSIONS: Care and technical assistance of long-term HMV children need assessment, planning, and resources. A wide variability in pattern of HMV was found throughout Italy. An Italian national ventilation program, as well as a national registry, could be useful in improving the care of these often critically ill children

Incidence and prognosis of ventilator-associated pneumonia in critically ill patients with covid-19: A multicenter study

The primary objective of this multicenter, observational, retrospective study was to assess the incidence rate of ventilator-associated pneumonia (VAP) in coronavirus disease 2019 (COVID-19) patients in intensive care units (ICU). The secondary objective was to assess predictors of 30-day case-fatality of VAP. From 15 February to 15 May 2020, 586 COVID-19 patients were admitted to the participating ICU. Of them, 171 developed VAP (29%) and were included in the study. The incidence rate of VAP was of 18 events per 1000 ventilator days (95% confidence intervals [CI] 16–21). Deep respiratory cultures were available and positive in 77/171 patients (45%). The most frequent organisms were Pseudomonas aeruginosa (27/77, 35%) and Staphylococcus aureus (18/77, 23%). The 30-day case-fatality of VAP was 46% (78/171). In multivariable analysis, septic shock at VAP onset (odds ratio [OR] 3.30, 95% CI 1.43–7.61, p = 0.005) and acute respiratory distress syndrome at VAP onset (OR 13.21, 95% CI 3.05–57.26, p &lt; 0.001) were associated with fatality. In conclusion, VAP is frequent in critically ill COVID-19 patients. The related high fatality is likely the sum of the unfavorable prognostic impacts of the underlying viral and the superimposed bacterial diseases

Metallicity Distribution of Galactic Halo Field RR Lyræ, and the Effect of Metallicity on Their Pulsation Properties

We present our analysis of a large sample (over 150k) of candidate Galactic RR Lyrae (RRL) stars for which we derived high quality photometry at UV, optical and infrared wavelengths, using data from publicly available surveys. For a sub-sample of these stars (∼ 2,400 fundamental mode field RRLs) we have measured their individual metallicity using the ΔS method, resulting in the largest and most homogeneous spectroscopic data set collected for RRLs. We use this sample to study the metallicity distribution in the Galactic Halo, including the long-standing problem of the Oosterhoff dichotomy among Galactic globular clusters. We also analyze the dependence of their pulsation properties, and in particular the shape of their infrared light curves, from their [Fe/H] abundance

TNFA and IL10 Gene Polymorphisms are not Associated with Periodontitis in Brazilians

IL-10 and TNF-α are cytokines that have complex and opposing roles in the inflammatory responses. G/A polymorphisms at position –1082 of IL10 and –308 of TNFA genes have been reported to influence the expression of IL-10 and TNF-α, respectively. The aim of this study was to investigate the association between the IL10 (-1082) and TNFA (- 308) gene polymorphisms with different clinical forms or severity of periodontitis in a sample of Brazilian individuals. DNA was obtained from oral swabs of 165 Brazilian individuals, which were divided into three groups: individuals with chronic periodontitis, aggressive periodontitis and individuals without clinical evidence of periodontitis. Evaluation of IL10 and TNFA polymorphisms was performed by RFLP analysis. Statistical analysis of data was performed using the χ2 likelihood ratio and Fisher`s exact test. No significant differences in the genotype and allele distribution of either IL10 or TNFA were observed among individuals with different clinical forms or with different degrees of severity of periodontitis. Moreover, combined analysis of IL10 and TNFA polymorphisms did not show any association with periodontal status. As conclusion, the IL10 and TNFA gene promoter polymorphisms investigated are not associated with different clinical forms of periodontitis or with severity of the disease in the Brazilian population polymorphisms

Vaccination with Recombinant Microneme Proteins Confers Protection against Experimental Toxoplasmosis in Mice

Toxoplasmosis, a zoonotic disease caused by Toxoplasma gondii, is an important public health problem and veterinary concern. Although there is no vaccine for human toxoplasmosis, many attempts have been made to develop one. Promising vaccine candidates utilize proteins, or their genes, from microneme organelle of T. gondii that are involved in the initial stages of host cell invasion by the parasite. In the present study, we used different recombinant microneme proteins (TgMIC1, TgMIC4, or TgMIC6) or combinations of these proteins (TgMIC1-4 and TgMIC1-4-6) to evaluate the immune response and protection against experimental toxoplasmosis in C57BL/6 mice. Vaccination with recombinant TgMIC1, TgMIC4, or TgMIC6 alone conferred partial protection, as demonstrated by reduced brain cyst burden and mortality rates after challenge. Immunization with TgMIC1-4 or TgMIC1-4-6 vaccines provided the most effective protection, since 70% and 80% of mice, respectively, survived to the acute phase of infection. In addition, these vaccinated mice, in comparison to non-vaccinated ones, showed reduced parasite burden by 59% and 68%, respectively. The protective effect was related to the cellular and humoral immune responses induced by vaccination and included the release of Th1 cytokines IFN-γ and IL-12, antigen-stimulated spleen cell proliferation, and production of antigen-specific serum antibodies. Our results demonstrate that microneme proteins are potential vaccines against T. gondii, since their inoculation prevents or decreases the deleterious effects of the infection

Characterization of Xenopus Tissue Inhibitor of Metalloproteinases-2: A Role in Regulating Matrix Metalloproteinase Activity during Development

Frog metamorphosis is totally dependent on thyroid hormone (T3) and mimics the postembryonic period around birth in mammals. It is an excellent model to study the molecular basis of postembryonic development in vertebrate. We and others have shown that many, if not all, matrix metalloproteinases (MMPs), which cleave proteins of the extracellular matrix as well as other substrates, are induced by T3 and important for metamorphosis. MMP activity can be inhibited by tissue inhibitors of metalloproteinase (TIMPs). There are 4 TIMPs in vertebrates and their roles in postembryonic development are poorly studied.We analyzed the TIMP2 genes in Xenopus laevis and the highly related species Xenopus tropicalis and discovered that TIMP2 is a single copy gene in Xenopus tropicalis as in mammals but is duplicated in Xenopus laevis. Furthermore, the TIMP2 locus in Xenopus tropicalis genome is different from that in human, suggesting an evolutionary reorganization of the locus. More importantly, we found that the duplicated TIMP2 genes were similarly regulated in the developing limb, remodeling intestine, resorbing tail during metamorphosis. Unexpectedly, like its MMP target genes, the TIMP2 genes were upregulated by T3 during both natural and T3-induced metamorphosis.Our results indicate that TIMP2 is highly conserved among vertebrates and that the TIMP2 locus underwent a chromosomal reorganization during evolution. Furthermore, the unexpected upregulation of TIMP2 genes during metamorphosis suggests that proper balance of MMP activity is important for metamorphosis