From rural house to “villa of delights”: knowledge and conservation of Villa Murat in the Sorrento peninsula

[EN] Thanks to an innovative territorial plan, drafted with the coordination of Roberto Pane and Luigi Piccinato and approved in 1987, many exceptional examples of vernacular architecture have been preserved in the Sorrento peninsula. Some of these, initially built as rural houses inserted in agricultural estates, have been transformed over the centuries by subsequent aggregation of volumes, into more complex structures, in which agricultural and residential usage have long coexisted. This is what happened to Villa Rossi, later known as Villa Murat, in the municipality of Massa Lubrense: an initial nucleus, dating back to the 17th century, was expanded during the following century, adding volumes and regularizing an initially spontaneous architecture. Despite such transformations, Villa Murat still retains some constructive features which are typical of vernacular architecture of the Sorrento and Amalfi coasts, such as extradosed vaults covered with beaten lapillus or the loggias facing the sea. The villa, which in the meantime had become property of the Rossi family, intertwined major historical events at the beginning of the 19th century, having become the headquarters of king Gioacchino Murat - hence its name - during the “Capture of Capri” against the British in 1808. Villa Murat, located in the quiet hamlet called Annunziata, away from the centres that have undergone major alterations, has still been preserved almost intact, even in the architectural surfaces, both the internal and external ones. For this reason a thorough research was possible, also thanks to an integrated survey with both aerial and terrestrial photogrammetry and laser scanning. The final aim, as the paper will show, is to develop a conservative design proposal, with targeted actions of conservation of architectural surfaces and structural consolidation, that will allow to keep the physical integrity of the villa and its authenticity without giving up a possible but compatible enhancement.Pane, A.; Catuogno, R.; Parente, M. (2022). From rural house to “villa of delights”: knowledge and conservation of Villa Murat in the Sorrento peninsula. Editorial Universitat Politècnica de València. 889-896. https://doi.org/10.4995/HERITAGE2022.2022.1436688989

GAMBARAN PENGETAHUAN MASYARAKAT TENTANG PHBS DALAM PENCEGAHAN COVID-19 DI DESA SOLEDUA NIAS SELATAN TAHUN 2021

Latar Belakang: Pengetahuan adalah suatu hasil dari rasa ingin “tahu” melalui proses sensoris, terutama pada mata dan telinga terhadap objek tertentu, pengetahuan masyarakat dalam mencegah transmisi penyebaran virus SARS-CoV-2 sangat berguna dalam menekan penularan virus. Penyakit covid-19 dapat dicegah diantaranya melakuakan perilaku hidup bersih dan sehat (PHBS) dengan memiliki pengetahuan, sikap dan tindakan. Tujuan: Tujuan penelitian ini untuk mengetahui gamabaran pengetahuan masyarakat dalam upaya pencegahan penyakit covid-19 di Desa Soledua Kecamatan Hilimegai Kabupaten Nias Selatan Tahun 2021. Metode: Penelitian ini menggunakan metode penelitian deskriptif. Teknik pengambilan sampel menggunakan Total Samping dengan jumlah sampel 114 responden. Alat ukur yang digunakan berupa kuesioner yang dibagikan kepada responden. Hasil: Berdasarkan hasil penelitian 53,5% masyarakat memiliki pengetahuan kurang, 33,3% berpengetahuan cukup dan 13,2% berpengetahuan baik. Kesimpulan: Diharapkan masyarakat dapat meningkatan pengetahuan, sikap, dan tindakan karena sangat penting dalam mencegah dan menghindari resiko penyakit covid-19

Description of Knowledge, Attitude, and Act Girls Teenager on Breast Self Examination (BSE) at Budi Murni 1 Chatolic Senior High School Medan in 2014

Breast self examination (BSE) is a way to do a woman to detect early abnormalities in the breast. Generally can be done by girls from the age of productive and has experienced changes the physical characteristics of primary and secondary. BSE goal is to determine whether there is abnormality such as breast cancer. The purpose of this study was to determine the internal factors, external factors, knowledge, attitudes and actions girls about breast self-examination (BSE). Type of research is descriptive research. In this study the number of population is all students of class XII SMA Katolik Budi Murni 1Medan as many as 200 people and sampled as many as 62 people. Sampling techniques using simple random sampling technique. Data collected by using a questionnaire. The data were analyzed using descriptive statistics. Based on the analysis of the study found adolescent knowledge about BSE were in the category of less than 61.3%. Student attitudes regarding BSE is in good enough category of 54.8%. And student action against BSE no middle category (74.2%). From these results it is expected that the school, parents, and caregivers around can play an active role in providing information about methods of early detection of breast self-examination and breast cancer in the students

Spiroplasma spp.: A Plant, Arthropod, Animal and Human Pathogen

Mollicutes is a class of smallest and free-living bacteria. They have no cell wall and their plasma membrane contains cholesterol; nevertheless, cellular organization does not differ from that of other prokaryotes. They are used as simple model systems for studying general biological problems, such as those concerning membrane structure and functions, symbiosis between arthropods and microrganisms, animal and plant pathogens. Mollicutes includes the family of Spiroplamataceae, which contains Spiroplasma genus, a group of species associated, in different manner, with arthropods (insects, mites, crustaceans). Spiroplasma species can be commensals or parasites and even be involved in more close symbiosis, such as synergism or mutualism. Out of 38 described Spiroplasma species, only three have been associated with plant diseases and three with arthropod diseases. Moreover, some species have been related to animal diseases, such as transmissible spongiform encephalopathy (TSE), and their role in human disease has been assessed. The chapter describes the taxonomic situation of the genus and reports the most important diseases due to the presence of Spiroplasma in different living organisms with special emphasis on citrus in which it causes one of the most economically damaging infectious diseases in a number of citrus growing areas worldwide

Lower apoptosis rate in human cumulus cells after administration of recombinant luteinizing hormone to women undergoing ovarian stimulation for in vitro fertilization procedures.

Objective To investigate the effects of recombinant (r-) LH supplementation in “low responder” patients undergoing ovarian stimulation with r-FSH for an IVF program. The apoptosis rate in cumulus cells was used as an indicator of oocyte quality. Design Comparison of the rate of DNA fragmentation and caspase-3 activity in cumulus cells in women stimulated with r-LH and r-FSH, versus patients treated with r-FSH alone (control). Setting In vitro fertilization (IVF) laboratory. Patient(s) Forty patients undergoing assisted fertilization programs treated with a GnRH agonist, or r-FSH treatment begun on day 3 of the cycle (control). In the r-LH group, from day 8 of gonadotropin stimulation, 150 IU per day of r-LH were administered. Intervention(s) Terminal deoxynucleotidyl transferase-mediated digoxigenin-deoxyuridine-triphosphate (dUTP) nick-end labeling (TUNEL) assay, and anti-caspase-3 cleaved immunoassay, to detect apoptosis in human cumulus cells. Main Outcome Measure(s) Difference in DNA fragmentation rate between cumulus cells derived from r-LH treatment and cumulus cells derived from control patients. Result(s) No differences were observed between the two groups in the total amount of r-FSH administered and in the number of retrieved oocytes per patient. A statistically significant increase in the number of immature oocytes and in the E2 serum peak was observed in the control group. The number of transferred embryos was significantly higher in the r-LH group. Pregnancy and implantation rates were higher in the r-LH group, but without statistical significance. The apoptosis rate in cumulus cells was higher in the control group than in the r-LH group. Conclusion(s) This study suggests that supplementation with r-LH improves the chromatin quality of cumulus cells involved in the control of oocyte maturation

Gold-based drug encapsulation within a ferritin nanocage: X-ray structure and biological evaluation as a potential anticancer agent of the Auoxo3-loaded protein

Auoxo3, a cytotoxic gold(iii) compound, was encapsulated within a ferritin nanocage. Inductively coupled plasma mass spectrometry, circular dichroism, UV-Vis absorption spectroscopy and X-ray crystallography confirm the potential-drug encapsulation. The structure shows that naked Au(i) ions bind to the side chains of Cys48, His49, His114, His114 and Cys126, Cys126, His132, His147. The gold-encapsulated nanocarrier has a cytotoxic effect on different aggressive human cancer cells, whereas it is significantly less cytotoxic for non-tumorigenic cells

DESIGNING BEACHSIDE RESORT AT PANTAI INDAH KALANGAN, CENTRAL TAPANULI WITH VERNACULAR ARCHITECTURE APPROACHMENT

Central Tapanuli is known for many stunning tourist locations, mainly its stretch of beach so that it becomes a great potential as an area of tourism with adequate accommodation as well as resorts. Some problems in the design resort that attract the attention and interest of tourist to come, how to design space programs according to human needs in it, and how to design buildings that are responsive to local climate, cultural and social conditions. From these problems, it is intended to create resorts with a variety of recreational facilities, design inner spaces and outdoor spaces according to resorts needs and local wisdom, and can introduce local culture to foreign tourists through resort design. Therefore, the research conducted by collecting existing location information, literature studies, comparative studies, and field surveys. After doing each step above, the result obtained, namely the design of the Beachside Resort using vernacular architecture, which is applied to each form of resort building, ranging from hotel, restaurant, cottages, spa and sauna, and other supporting facilities, using the types of the Central Tapanuli household, combined with the application of “Dalihan Natolu”. By obtaining these result, it is expected to become tourism locations and become a positive response in bringing back the traditional architecture of Central Tapanuli

FSH administration reduces significantly sperm apoptosis only in the case of high DFI value: a study in idiopathic dispermic patients

Introduction: In the last decades sperm DNA quality has been recognized as one of the most important markers of male reproductive potential (Lewis and Aitken, 2005; Ozmen, 2007; Tarozzi, 2007), in contrast to standard semen parameters as sperm density, motility and morphology, which do not act as powerful discriminators between fertile and infertile men. DNA damage in the male germ line is a major contributor to infertility, miscarriage and birth defects in the offspring. In animal models, it has been unequivocally demonstrated that the genetic integrity of the male germ line plays a major role in determining the normality of embryonic development. In humans, many studies showed that sperm DNA damage is associated with impaired embryo cleavage (8), higher miscarriage rates (9) and also with a significantly increased risk of pregnancy loss after in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) (10). Specifically, above a threshold of 30% of sperms with fragmented DNA, chances for pregnancy are close to zero, either by means of natural conception or intrauterine insemination (Spano M, 2000; Bungum M, 2007). Since there is a clear relationship between sperm DNA damage and poor assisted reproduction technology (ART) outcomes, efforts should be directed in developing treatments to improve sperm DNA quality to be introduced into clinical use. The aim of this observational study was to investigate the effects of r-FSH administration on sperm DNA fragmentation of iOAT patients undergoing ICSI, comparing the DNA fragmentation index (DFI) before and after 90 days of FSH therapy. Matherial and Methods: Fifty-three iOAT men, with a median age of 33,6 ± 7,6 years, referred to our clinics because of fertility problems after at least two years of natural attempts, were selected for the study. In all patients DNA fragmentation was evaluated sperm prior to treatment with 150 IU of recombinant human FSH (GONAL-f®, Merck Serono) three times at week for at least three months. Patients were re-evaluated after a 3-month period with semen analysis and DNA fragmentation. Sperm DNA fragmentation index (DFI) was investigated by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) in situ DNA nick end labelling (TUNEL) assay. Data were analysed using the paired t-test and chi-square as appropriate. A p-value <0.05 was considered statistically significant. Results: After 3 months of r-FSH treatment, no significant differences was observed between baseline and post therapy semen sample parameters including sperm count, motility, and the percentage of normal sperm forms. IThe percentage of sperm DNA fragmentation in the total of patients dropped from 20.8 ± 9.1 to 15.1 ± 8.9 (P < 0.05) (see Tab I). Interestingly, no statistical difference was found in sperm DFI when patients showed a baseline DFI ≤15% (10.5 ± 4.2 vs 11.4 ± 4.5). We found an evident and statistically significant DFI reduction in patients with sperm baseline DFI value ≥15% (24.37 ± 9.6 vs 15.4 ± 4.6). Conclusion: Our data seems to demonstrate that FSH acts as a strong anti-apoptotic agent in reducing DNA fragmentation in iOAT patients. The therapy may be a specific pretreatment for infertile male partners of couples undergoing ICSI, specifically in the case that basal DFI is higher than 15%, reducing the percentage of spermatozoa with DNA integrity anomalies suggesting a positive effect on the reproductive outcome

Il rilievo integrato del castello-recinto di Pesche (IS): conoscenza e strategie di valorizzazione di un insediamento fortificato in Molise

Perched on the slopes of San Marco Mount, in a perfect symbiosis of architecture and landscape, there is the small village of Pesche in the province of Isernia, which traces its origins back to the 5th-6th centuries, when the steep natural slope was chosen as the site for the construction of a safe village. On the top of the site, the ruins of the so-called Castrum Pesclarum emerge, evidence of a particular type of fortified structure in Molise, that of the castle-enclosure. Built as early as the Norman era to reinforce the defence and transformed over the following centuries due to damage caused by natural disasters and human actions, the small fortified citadel is haracterised by a perimeter marked by curtain walls that follow the natural slope, interspersed with towers, while its interior contains small rooms placed side by side and built using the typical limestone of the area. The present contribution intends to show the current state of conservation of the fortified citadel of Pesche, outlining the methodology followed in the study of the site, and then proposing a possible enhancement strategy. The work was conducted starting from an integrated instrumental survey, based on laser scanning and aerial digital photogrammetry with a drone, thus showing the efficiency of these systems not only at the single architectural scale, but also at the urban scale, even in complex sites such as that of Pesche. The combination of these two techniques has made it possible to obtain an accurate knowledge of a large part of the site, thus making it possible to propose design strategies for its conservation and enhancement

Early ultrasonographic finding of septic thrombophlebitis is the main indicator of central venous catheter removal to reduce infection-related mortality in neutropenic patients with bloodstream infection.

BACKGROUND: Septic thrombophlebitis increases patient morbidity and mortality following metastatic infections, pulmonary emboli, and/or septic shock. Central venous catheter (CVC) removal for occult septic thrombophlebitis challenges current strategy in neutropenic patients. PATIENTS AND METHODS: We prospectively evaluated infection-related mortality in 100 acute leukemia patients, with CVC-related bloodstream infection (CRBSI) after chemotherapy, who systematically underwent ultrasonography to identify the need for catheter removal. Their infection-related mortality was compared with that of a historical cohort of 100 acute leukemia patients, with CRBSI after chemotherapy, managed with a clinically driven strategy. Appropriate antimicrobial therapy was administered in all patients analyzed. RESULTS: In the prospective series, 30/100 patients required catheter removal for ultrasonography-detected septic thrombophlebitis after 1 median day from BSI onset; 70/100 patients without septic thrombophlebitis retained their CVC. In the historical cohort, 60/100 patients removed the catheter (persistent fever, 40 patients; persistent BSI, 10 patients; or clinically manifest septic thrombophlebitis, 10 patients) after 8 median days from BSI onset; 40/100 patients retained the CVC because they had not clinical findings of complicated infection. At 30 days median follow-up, one patient died for infection in the ultrasonography-assisted group versus 17 patients in the historical cohort (P &lt; 0.01). With the ultrasonography-driven strategy, early septic thrombophlebitis detection and prompt CVC removal decrease infection-related mortality, whereas clinically driven strategy leads to inappropriate number, reasons, and timeliness of CVC removal. CONCLUSION: Ultrasonography is an easy imaging diagnostic tool enabling effective and safe management of patients with acute leukemia and CRBSI