Metabolic responses to high pCO2 conditions at a CO2 vent site in juveniles of a marine isopod species assemblage

We are starting to understand the relationship between metabolic rate responses and species' ability to respond to exposure to high pCO2. However, most of our knowledge has come from investigations of single species. The examination of metabolic responses of closely related species with differing distributions around natural elevated CO2 areas may be useful to inform our understanding of their adaptive significance. Furthermore, little is known about the physiological responses of marine invertebrate juveniles to high pCO2, despite the fact they are known to be sensitive to other stressors, often acting as bottlenecks for future species success. We conducted an in situ transplant experiment using juveniles of isopods found living inside and around a high pCO2 vent (Ischia, Italy): the CO2 'tolerant' Dynamene bifida and 'sensitive' Cymodoce truncata and Dynamene torelliae. This allowed us to test for any generality of the hypothesis that pCO2 sensitive marine invertebrates may be those that experience trade-offs between energy metabolism and cellular homoeostasis under high pCO2 conditions. Both sensitive species were able to maintain their energy metabolism under high pCO2 conditions, but in C. truncata this may occur at the expense of [carbonic anhydrase], confirming our hypothesis. By comparison, the tolerant D. bifida appeared metabolically well adapted to high pCO2, being able to upregulate ATP production without recourse to anaerobiosis. These isopods are important keystone species; however, given they differ in their metabolic responses to future pCO2, shifts in the structure of the marine ecosystems they inhabit may be expected under future ocean acidification conditions

Binding assays of native IslA and the truncated form IslA3 with non induced CW28 cells

Molecular weight control (M). Gel deals with native IslA (line 1) while gel with IslA3 (line 1). In both gels line 2 refers to the non induced CW28 cells; line 3 refers to the washed non induced cells after contact with the protein; and line 4 refers to the protein solution after contact with non induced cells.<p><b>Copyright information:</b></p><p>Taken from "Functional role of the additional domains in inulosucrase (IslA) from CW28"</p><p>http://www.biomedcentral.com/1471-2091/9/6</p><p>BMC Biochemistry 2008;9():6-6.</p><p>Published online 31 Jan 2008</p><p>PMCID:PMC2270844.</p><p></p

PLANEACIÓN DIDÁCTICA DE LA ASIGNATURA DE QUÍMICA I CBU 2015. SEMESTRE: 2017B

GUÍA DIDÁCTICA / PLANEACIÓN DIDÁCTICA (NMS