Effects of Clonal Reproduction of Evolutionary Lag and Evolutionary Rescue

Evolutionary lag—the difference between mean and optimal phenotype in the current environment—is of keen interest in light of rapid environmental change. Many ecologically important organisms have life histories that include stage structure and both sexual and clonal reproduction, yet how stage structure and clonality interplay to govern a population’s rate of evolution and evolutionary lag is unknown. Effects of clonal reproduction on mean phenotype partition into two portions: one that is phenotype dependent, and another that is genotype dependent. This partitioning is governed by the association between the nonadditive genetic plus random environmental component of phenotype of clonal offspring and their parents. While clonality slows phenotypic evolution toward an optimum, it can dramatically increase population survival after a sudden step change in optimal phenotype. Increased adult survival slows phenotypic evolution but facilitates population survival after a step change; this positive effect can, however, be lost given survival-fecundity trade-offs. Simulations indicate that the benefits of increased clonality under environmental change greatly depend on the nature of that change: increasing population persistence under a step change while decreasing population persistence under a continuous linear change requiring de novo variation. The impact of clonality on the probability of persistence for species in a changing world is thus inexorably linked to the temporal texture of the change they experience

National income inequality predicts cultural variation in mouth to mouth kissing

Romantic mouth-to-mouth kissing is culturally widespread, although not a human universal, and may play a functional role in assessing partner health and maintaining long-term pair bonds. Use and appreciation of kissing may therefore vary according to whether the environment places a premium on good health and partner investment. Here, we test for cultural variation (13 countries from six continents) in these behaviours/attitudes according to national health (historical pathogen prevalence) and both absolute (GDP) and relative wealth (GINI). Our data reveal that kissing is valued more in established relationships than it is valued during courtship. Also, consistent with the pair bonding hypothesis of the function of romantic kissing, relative poverty (income inequality) predicts frequency of kissing across romantic relationships. When aggregated, the predicted relationship between income inequality and kissing frequency (r=.67, BCa 95%CI[.32,.89]) was over five times the size of the null correlations between income inequality and frequency of hugging/cuddling and sex. As social complexity requires monitoring resource competition among large groups and predicts kissing prevalence in remote societies, this gesture may be important in the maintenance of long-term pair bonds in specific environments

Ambient mass spectrometry technologies for the detection of falsified drugs

Increased globalization of the pharmaceutical market has facilitated the unobstructed and fast spread of poor-quality medicines. Poor-quality medicines include spurious/falsely-labeled/falsified/counterfeit drugs (those that are deliberately and fraudulently mislabeled with respect to content and/or origin), substandard drugs (legitimate drugs that do not meet their quality specifications), and degraded medicines (good quality pharmaceuticals that suffered from deterioration caused by improper storage or distribution). Consumption of poor-quality pharmaceuticals is likely to increase morbidity and mortality.Moreover, poor-quality drugs can also contribute to the development of resistance to anti-infective medicines and decrease the quality of health care received by patients. To assess the true prevalence of poor quality drugs, tiered technology approaches enabling the testing of drug samples collected at points of sale are required, thus ensuring public health standards. High throughput and high resolution ambient mass spectrometry techniques allow investigation of pharmaceuticals with minimal or no sample preparation, thus possessing capabilities to survey a large number of drug samples for their authenticity.Fil: Culzoni, Maria Julia. Georgia Institute Of Techology; Estados Unidos. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Santa Fe; ArgentinaFil: Dwivedi, Prabha. Georgia Institute Of Techology; Estados UnidosFil: Green, Michael D.. Centers For Disease Control And Prevention. National Center For Infectious Diseases. Division Of Parasitic Diseases; Estados UnidosFil: Newton, Paul. Mahosot Hospital; Laos. University of Oxford; Reino UnidoFil: Fernandez, Facundo. Georgia Institute Of Techology; Estados Unido

Understanding shock dynamics in the inner heliosphere with modeling and type II radio data: A statistical study

We study two methods of predicting interplanetary shock location and strength in the inner heliosphere: (1) the ENLIL simulation and (2) the kilometric type II (kmTII) prediction. To evaluate differences in the performance of the first method, we apply two sets of coronal mass ejections (CME) parameters from the cone-model fitting and flux-rope (FR) model fitting as input to the ENLIL model for 16 halo CMEs. The results show that the ENLIL model using the actual CME speeds from FR-fit provided an improved shock arrival time (SAT) prediction. The mean prediction errors for the FR and cone-model inputs are 4.90±5.92 h and 5.48±6.11 h, respectively. A deviation of 100 km s−1 from the actual CME speed has resulted in a SAT error of 3.46 h on average. The simulations show that the shock dynamics in the inner heliosphere agrees with the drag-based model. The shock acceleration can be divided as two phases: a faster deceleration phase within 50 Rs and a slower deceleration phase at distances beyond 50 Rs. The linear-fit deceleration in phase 1 is about 1 order of magnitude larger than that in phase 2. When applying the kmTII method to 14 DH-km CMEs, we found that combining the kmTII method with the ENLIL outputs improved the kmTII prediction. Due to a better modeling of plasma density upstream of shocks and the kmTII location, we are able to provide a more accurate shock time-distance and speed profiles. The mean kmTII prediction error using the ENLIL model density is 6.7±6.4 h; it is 8.4±10.4 h when the average solar wind plasma density is used. Applying the ENLIL density has reduced the mean kmTII prediction error by ∼2 h and the standard deviation by 4.0 h. Especially when we applied the combined approach to two interacting events, the kmTII prediction error was drastically reduced from 29.6 h to −4.9 h in one case and 10.6 h to 4.2 h in the other. Furthermore, the results derived from the kmTII method and the ENLIL simulation, together with white-light data, provide a valuable validation of shock formation location and strength. Such information has important implications for solar energetic particle acceleration.Fil: Xie, H.. NASA. Goddard Space Flight Center; Estados Unidos. Department of Physics. Catholic University of America; Estados UnidosFil: St. Cyr, O.C.. NASA. Goddard Space Flight Center; Estados UnidosFil: Gopalswamy, N.. NASA. Goddard Space Flight Center; Estados UnidosFil: Odstrcil, D.. George Mason University. Department of Computational and Data Sciences; Estados UnidosFil: Cremades Fernandez, Maria Hebe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional de Mendoza; Argentin

Comparison of stresses in 3D v. 2D geomechanical modelling of salt structures in the Tarfaya Basin, West African coast

We predict stresses and strains in the Tarfaya salt basin on the West African coast using a 3D static geomechanical model and compare the results against a simplified 2D plane-strain model. Both models are based on present-day basin geometries, are drained, and use a poroelastic description for the sediments and visco-plastic description for salt. We focus on a salt diapir, where an exploratory well has been drilled crossing a major fault. The 3D model shows a significant horizontal stress reduction in sediments at the top of the diapir, validated with measured data later obtained from the well. The 2D model predicts comparable stress reduction in sediments at the crest of the diapir. However, it shows a broader area affected by the stress reduction, overestimating its magnitude by as much as 1.5 MPa. Both models predict a similar pattern of differential displacement in sediments along both sides of the major fault, above the diapir. These displacements are the main cause of horizontal stress reduction detected at the crest of the diapir. Sensitivity analysis in both models shows that the elastic parameters of the sediments have a minimal effect on the stress-strain behaviour. In addition, the 2D sensitivity analysis concludes that the main factors controlling stress and strain changes are the geometry of the salt and the difference in rock properties between encasing sediments and salt. Overall, our study demonstrates that carefully built 2D models at the exploration stage can provide stress information and useful insights comparable to those from more complex 3D geometrie

Synthetic antigenic determinants of clavulanic acid induce dendritic cell maturation and specific T cell proliferation in patients with immediate hypersensitivity reactions

Background Immediate drug hypersensitivity reactions (IDHRs) to clavulanic acid (CLV) have increased in the last decades due to a higher consumption alongside amoxicillin (AX). Due to its chemical instability, diagnostic procedures to evaluate IDHRs to CLV are difficult, and current in vitro assays do not have an optimal sensitivity. The inclusion of the specific metabolites after CLV degradation, which are efficiently recognised by the immune system, could help to improve sensitivity of in vitro tests. Methods Recognition by dendritic cells (DCs) of CLV and the synthetic analogues of two of its hypothesised antigenic determinants (ADs) was evaluated by flow cytometry in 27 allergic patients (AP) and healthy controls (HC). Their ability to trigger the proliferation of T cells was also analysed by flow cytometry. Results The inclusion of synthetic analogues of CLV ADs, significantly increased the expression of maturation markers on DCs from AP compared to HC. A different recognition pattern could be observed with each AD, and, therefore, the inclusion of both ADs achieves an improved sensitivity. The addition of synthetic ADs analogues increased the proliferative response of CD4+Th2 compared to the addition of native CLV. The combination of results from both ADs increased the sensitivity of proliferative assays from 19% to 65% with a specificity higher than 90%. Conclusions Synthetic ADs from CLV are efficiently recognised by DCs with ability to activate CD4+Th2 cells from AP. The combination of analogues from both ADs, significantly increased the sensitivity of DC maturation and T-cell proliferation compared to native CLV.This work has been supported by Institute of Health ‘Carlos III’ (ISCIII) of the Ministry of Economy and Competitiveness (MINECO) (grants co-funded by European Regional Development Fund: PI15/01206, PI17/01237, PI18/00095, PI20/01734, RETICS ARADYAL RD16/0006/0001); Andalusian Regional Ministry of Health (grants PI-0241-2016, PE-0172-2018, PI-0127-2020); Spanish Ministerio de Ciencia e Innovación (Proyectos de I+D+I «Programación Conjunta Internacional», EuroNanoMed 2019 (PCI2019-111825-2), Ministerio de Ciencia y Educación (PID2019-104293GB-I00), Instituto de Salud Carlos III (ISCIII) of MINECO (RD16/0006/0012), Junta de Andalucía ( PY20_00384 ). AA and NPS hold Senior Postdoctoral Contracts (RH-0099-2020 and RH-0085-2020) from Andalusian Regional Ministry of Health (cofunded by European Social Fund (ESF): ‘Andalucía se mueve con Europa’). JLP holds a Sara Borrell fellowship (CD19/00250) by ISCIII of MINECO (cofunded by ESF, “El FSE invierte en futuro”). GB holds a ‘Juan Rodes’ contract (JR18/00054) by ISCIII of MINECO (cofunded by ESF). MIM holds a ‘Miguel Servet II’ grant (CPII20/00028) by ISCIII of MINECO (cofunded by ESF). ML holds a ‘Rio Hortega’ contract (CM20/00210) by ISCIII of MINECO (cofunded by ESF). CM holds a ‘Nicolas Monardes’ research contract by Andalusian Regional Ministry Health (RC-0004-2021). NMR experiments for characterizing molecule structures have been performed in the ICTS ‘NANBIOSIS’, by the U28 Unit at the Andalusian Centre for Nanomedicine and Biotechnology (BIONAND). Funding for open access charge: Universidad de Málag

Los/as estudiantes de educación social y trabajo social ante la atención a personas mayores: Intereses profesionales

La gerontología constituye uno de los ámbitos de intervención en auge para los titulados/as en Educación Social y Trabajo Social. Esta investigación tiene como objetivo identificar las preferencias vocacionales de estos estudiantes en relación a la intervención con personas mayores, a partir de un estudio cuasi-experimental, descriptivo y transversal, en base a la administración de un cuestionario. Globalmente, los resultados indican un escaso interés de los estudiantes por el colectivo de las personas mayores y un incremento de las preferencias por este grupo de edad en el último curso

Establecimiento del sistema de regeneración por embriogénesis somática de Azadirachta Indica A. Juss.

Azadirachta indica, es una planta con múltiples aplicaciones tanto forestal como farmacológica. Por ende, el establecimiento del sistema de cultivo in vitro por embriogénesis somática ofrece diversas y variadas ventajas, tales como obtener plantas altamente productivas en metabolitos. En este estudio, se utilizaron secciones foliares y cotiledonares, inducidas en medios MS (1962) suplementados con: BAP sólo y combinado con ANA / 2,4-D, TDZ sólo y con ABA. La regeneración fue con MS sólo o con K + AIA y BAP + AIA. Como resultado se estableció un sistema eficiente con secciones de cotiledones, observándose organogénesis a bajas concentraciones de BAP, mientras a altos niveles de BAP (2,5 mg.L-1), así como con TDZ + ABA (0,02 + 1 mg.L-1) respectivamente favorecieron la embriogénesis somática primaria y secundaria en un 96 % y 71 % respectivamente. La regeneración fue 71 % con MS, mientras que el enraizamiento fue de 86,67 % con MS½, obteniéndose plantas completas a corto plazo.Azadirachta indica, is a plant with multiple forest and pharmacological application. Therefore, the establishment of in vitro culture system for somatic embryogenesis offers several distinct advantages such as obtaining highly productive plant metabolites. In this study, were used sections cotyledon and leaf, induced on MS medium (1962) supplemented with: BAP alone and combined with NAA / 2,4-D, TDZ alone and ABA. Regeneration was with MS alone or with K + BAP + IAA and IAA. As a result was established an efficient system with cotyledon sections, being observed organogenesis at low concentrations of BAP, while high levels of BAP (2.5 mg.L-1) with 96 % and TDZ + ABA (0.02 + 1 mg.L-1) with 71 %, favoring the primary and secondary somatic embryogenesis. Regeneration was 71 % with MS; rooting was 86.67 % with MS½, presenting whole plants obtained short term

Evaluation of Community-Academic Partnership: Lessons from Latinos in a Network for Cancer Control

Established in 2002, Latinos in a Network for Cancer Control (LINCC) is a community-academic network supported by the Centers for Disease Control and Prevention and the National Cancer Institute. LINCC includes \u3e130 individuals from 65 community and academic organizations committed to reducing cancer-related health disparities. Using an empirically derived systems model—the Bergen Model of Collaborative Functioning—as the analytic frame, we interviewed 19 partners to identify challenges and successful processes. Findings indicated that sustained partner interaction created “meaningful relationships” that were routinely called upon for collaboration. The leadership was regarded positively on vision, charisma, and capacity. Limitations included over-reliance on a single leader. Suggestions supported more delegation of decision-making, consistent communication, and more equitable resource distribution. The study highlighted new insights into dynamics of collaboration: Greater inclusiveness of inputs (partners, finances, mission) and loosely defined roles and structure produced strong connections but less network-wide productivity (output). Still, this profile enabled the creation of more tightly defined and highly productive sub-groups, with clear goals and roles but less inclusive of inputs than the larger network. Important network outputs included practice-based research publications, cancer control intervention materials, and training to enhance the use of evidence-based interventions, as well as continued and diversified funding