The hydro-morphological index of diversity: a tool for describing habitat heterogeneity in river engineering projects

We present a new hydro-morphological index of diversity (HMID), a tool aimed for use in river engineering projects and firstly developed at gravel-bed streams in Switzerland, but intended for a broader use. We carried out field work with extensive hydraulic and geomorphic data collection, conducted correlation analysis with hydro-morphological variables, formulated the HMID, and analyzed the correlation between HMID and a visual habitat assessment method. The HMID is calculated by means of the coefficient of variation of the hydraulic variables flow velocity and water depth, which have been demonstrated to sufficiently represent the hydro-morphological heterogeneity of alpine gravel-bed stream reaches. Based on numerical modeling, the HMID can be calculated easily for a comparison of different alternatives in river engineering projects and thus achieves predictive power for design decisions. HMID can be applied at a reach-related scale in engineering programs involving geomorphic measures that aim at the enhancement of habitat heterogeneity of a stream. However, the application of HMID has to be integrated with evaluations of the long-term streambed evolvements that are considered at a catchment scale and strongly related to the sediment regime of the stream under stud

Extremely low frequency magnetic field induces human neuronal differentiation through NMDA receptor activation

Magnetic fields with different frequency and intensity parameters exhibit a wide range of effects on different biological models. Extremely low frequency magnetic field (ELF MF) exposure is known to augment or even initiate neuronal differentiation in several in vitro and in vivo models. This effect holds potential for clinical translation into treatment of neurodegenerative conditions such as autism, Parkinson's disease and dementia by promoting neurogenesis, non-invasively. However, the lack of information on underlying mechanisms hinders further investigation into this phenomenon. Here, we examine involvement of glutamatergic Ca2+ channel, N-methyl-D-aspartate (NMDA) receptors in the process of human neuronal differentiation under ELF MF exposure. We show that human neural progenitor cells (hNPCs) differentiate more efficiently under ELF MF exposure in vitro, as demonstrated by the abundance of neuronal markers. Furthermore, they exhibit higher intracellular Ca2+ levels as evidenced by c-fos expression and more elongated mature neurites. We were able to neutralize these effects by blocking NMDA receptors with memantine. As a result, we hypothesize that the effects of ELF MF exposure on neuronal differentiation originate from the effects on NMDA receptors, which sequentially triggers Ca2+-dependent cascades that lead to differentiation. Our findings identify NMDA receptors as a new key player in this field that will aid further research in the pursuit of effect mechanisms of ELF MFs.European Molecular Biology Organization Short Term Fellowships, ASTF 7502. This work was partially supported by TUBITAK Projects under Grant No. 117Z864, Bogazici University Research Fund by Grant Number 6701. A.J. Salgado and A. Marote acknowledge the financial support from: Prémios Santa Casa Neurociências–Prize Melo e Castro for Spinal Cord Injury Research (MC-04/17); Portuguese Foundation for Science and Technology Pre–Doctoral fellowship to A. Marote PDE/BDE/113598/2015 and IF Development Grant to A. J. Salgado. This work is funded by national funds through FCT under the scope of grant reference TUBITAK/0007/2014. This article has been developed under the scope of the projects NORTE-01-0145-FEDER-000023, supported by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). This work has been funded by FEDER funds, through the Competitiveness Factors Operational Programme (COMPETE), and by National funds, through the Foundation for Science and Technology (FCT), under the scope of the project POCI-01-0145-FEDER-007038 and POCI-01-0145-FEDER-02920

Shifts in isotopic signatures of animals with complex life-cycles can complicate conclusions on cross-boundary trophic links

Stable isotopes (13C and 15N) are widely applied in studies of trophic links. We used this method to investigate the contribution of aquatic and terrestrial prey to the diet of riparian predatory arthropods in two mountain headwater streams in Colorado, USA. Aquatic and terrestrial prey and riparian predators were collected during summer 2009. To evaluate the reliability of conclusions based on stable isotope ratios, we compared the isotopic signatures of aquatic larval and terrestrial adult stages of three abundant stream insect species and assessed variation in mixing model estimates for spider diet composition under varying assumptions for trophic fractionation. Adult isotopic signatures of some aquatic prey species were indistinguishable from those of prey species with exclusively terrestrial life histories (stoneflies: 13C and 15N, chironomids: 13C). Other prey had distinctly aquatic isotopic signatures as both larvae and adults (a mayfly and a caddisfly). There was no evidence that prey with aquatic isotopic signatures contributed to the diet of the spiders near one stream. For the other stream, mixing model analysis suggested that chironomids were included in the diets of lycosid, linyphiid and liocranid spiders. Reliable estimates of the contributions of prey sources were compromised by the sensitivity of mixing models to assumptions on trophic fractionation and the presence of "isotopically cryptic” prey. This study emphasizes the importance of supporting isotope-based studies on cross-boundary trophic links with data on isotopic shifts in prey with complex life cycles and assessment of fractionation rates specific to the study syste

The hydro-morphological index of diversity: a tool for describing habitat heterogeneity in river engineering projects

We present a new hydro-morphological index of diversity (HMID), a tool aimed for use in river engineering projects and firstly developed at gravel-bed streams in Switzerland, but intended for a broader use. We carried out field work with extensive hydraulic and geomorphic data collection, conducted correlation analysis with hydro-morphological variables, formulated the HMID, and analyzed the correlation between HMID and a visual habitat assessment method. The HMID is calculated by means of the coefficient of variation of the hydraulic variables flow velocity and water depth, which have been demonstrated to sufficiently represent the hydro-morphological heterogeneity of alpine gravel-bed stream reaches.Based on numerical modeling, the HMID can be calculated easily for a comparison of different alternatives in river engineering projects and thus achieves predictive power for design decisions. HMID can be applied at a reach-related scale in engineering programs involving geomorphic measures that aim at the enhancement of habitat heterogeneity of a stream. However, the application of HMID has to be integrated with evaluations of the long-term streambed evolvements that are considered at a catchment scale and strongly related to the sediment regime of the stream under study

100 key questions to guide hydropeaking research

Hydropeaking has received increasing attention in the last years, but many knowledge gaps remain, potentially hampering effective policy and management efforts in rivers under such type of hydropower production. In this study, we collected open hydropeaking research questions from over 200 experts in river science, practice, and policy across the globe using an online survey available in five languages. We used a systematic method of determining expert consensus (Delphi method) to identify 100 core questions related to the following thematic fields: (i) hydrology, (ii) physico-chemical properties of water, (iii) river morphology and sedimentology, (iv) ecology and biology, (v) socio-economics and energy markets, (vi) policy and regulation, as well as (vii) management and mitigation measures. The consensus list of questions shall inform and guide researchers in focusing their efforts to foster a better science-policy interface, thereby improving the sustainability of peak-operating hydropower in a variety of settings

Biodiversität in Fliessgewässern

Vielfältige, naturnahe und dynamische Lebensräume sind eine wichtige Voraussetzung dafür, die Biodiversität in Fliessgewässern zu erhalten und zu fördern. Das vorliegende Merkblatt stellt die wichtigsten Faktoren für die Lebensraum- und Artenvielfalt vor und präsentiert Empfehlungen, mit welchen Massnahmen die Biodiversität erhöht werden kann

Biodiversité dans les cours d’eau

Des habitats diversifiés, dynamiques et proches de l’état naturel sont indispensables à la conservation et à l’amélioration de la biodiversité dans les cours d’eau. Cette fiche présente les principaux facteurs de la diversité des habitats et des espèces, ainsi que des mesures permettant d’accroître la biodiversit

100 key questions to guide hydropeaking research

As the share of renewable energy grows worldwide, flexible energy production from peak-operating hydropower and the phenomenon of hydropeaking have received increasing attention. In this study, we collected open research questions from 220 experts in river science, practice, and policy across the globe using an online survey available in six languages related to hydropeaking. We used a systematic method of determining expert consensus (Delphi method) to identify 100 high-priority questions related to the following thematic fields: (a) hydrology, (b) physico-chemical properties of water, (c) river morphology and sediment dynamics, (d) ecology and biology, (e) socio-economic topics, (f) energy markets, (g) policy and regulation, and (h) management and mitigation measures. The consensus list of high-priority questions shall inform and guide researchers in focusing their efforts to foster a better science-policy interface, thereby improving the sustainability of peak-operating hydropower in a variety of settings. We find that there is already a strong understanding of the ecological impact of hydropeaking and efficient mitigation techniques to support sustainable hydropower. Yet, a disconnect remains in its policy and management implementation.publishedVersio

CoronaHiT: high-throughput sequencing of SARS-CoV-2 genomes.

We present CoronaHiT, a platform and throughput flexible method for sequencing SARS-CoV-2 genomes (≤ 96 on MinION or > 96 on Illumina NextSeq) depending on changing requirements experienced during the pandemic. CoronaHiT uses transposase-based library preparation of ARTIC PCR products. Method performance was demonstrated by sequencing 2 plates containing 95 and 59 SARS-CoV-2 genomes on nanopore and Illumina platforms and comparing to the ARTIC LoCost nanopore method. Of the 154 samples sequenced using all 3 methods, ≥ 90% genome coverage was obtained for 64.3% using ARTIC LoCost, 71.4% using CoronaHiT-ONT and 76.6% using CoronaHiT-Illumina, with almost identical clustering on a maximum likelihood tree. This protocol will aid the rapid expansion of SARS-CoV-2 genome sequencing globally.The sequencing costs were funded by the COVID-19 Genomics UK (COG-UK) Consortium which is supported by funding from the Medical Research Council (MRC) part of UK Research & Innovation (UKRI), the National Institute of Health Research (NIHR) and Genome Research Limited, operating as the Wellcome Sanger Institute