Proposal and preliminary design for a high speed civil transport aircraft. Swift: A high speed civil transport for the year 2000

To meet the needs of the growing passenger traffic market in light of an aging subsonic fleet, a new breed of aircraft must be developed. The Swift is an aircraft that will economically meet these needs by the year 2000. Swift is a 246 passenger, Mach 2.5, luxury airliner. It has been designed to provide the benefit of comfortable, high speed transportation in a safe manner with minimal environmental impact. This report will discuss the features of the Swift aircraft and establish a solid, foundation for this supersonic transport of tomorrow

Antimicrobial efficacy against Pseudomonas aeruginosa biofilm formation in a three-dimensional lung epithelial model and the influence of fetal bovine serum

In vitro models that mimic in vivo host-pathogen interactions are needed to evaluate candidate drugs that inhibit bacterial virulence traits. We established a new approach to study Pseudomonas aeruginosa biofilm susceptibility on biotic surfaces, using a three-dimensional (3-D) lung epithelial cell model. P. aeruginosa formed antibiotic resistant biofilms on 3-D cells without affecting cell viability. The biofilm-inhibitory activity of antibiotics and/or the anti-biofilm peptide DJK-5 were evaluated on 3-D cells compared to a plastic surface, in medium with and without fetal bovine serum (FBS). In both media, aminoglycosides were more efficacious in the 3-D cell model. In serum-free medium, most antibiotics (except polymyxins) showed enhanced efficacy when 3-D cells were present. In medium with FBS, colistin was less efficacious in the 3-D cell model. DJK-5 exerted potent inhibition of P. aeruginosa association with both substrates, only in serum-free medium. DJK-5 showed stronger inhibitory activity against P. aeruginosa associated with plastic compared to 3-D cells. The combined addition of tobramycin and DJK-5 exhibited more potent ability to inhibit P. aeruginosa association with both substrates. In conclusion, lung epithelial cells influence the efficacy of most antimicrobials against P. aeruginosa biofilm formation, which in turn depends on the presence or absence of FBS

Parenting in the context of driving: Spanish adaptation of the Family Climate for Road Safety (FCRSS) for parents and children

The Family Climate for Road Safety Scale (FCRSS; Taubman – Ben-Ari & Katz – Ben-Ami, 2013) is a comprehensive measure originally developed in Israel to assess parent-children relations in the specific context of driving. The scale consists of seven dimensions: Modelling, Feedback, Communication, Monitoring, Messages, Limits, and Non-commitment to Safety. While the original FCRSS examines the young drivers’ perception across the seven domains, a version applicable to parents has also been developed by the same authors. The current study investigates the validity and reliability of the FCRSS-Spain for both parents and young drivers. A total of 377 parents (199 fathers and 178 mothers) and 243 of their children (143 daughters and 100 sons) responded to the FCRSS-Spain versions and provided sociodemographic data. In addition, the young drivers completed the Spanish version of the Multidimensional Driving Style Inventory (MDSI-Spain). Results from exploratory structural equation modelling (ESEM) indicate that six out of the seven FCRSS domains were replicable among Spanish drivers. The Messages dimension did not emerge as a consistent factor in the FCRSS for either parents or young drivers. All six factors demonstrated good internal consistency reliability (ordinal alpha coefficients exceeding 0.70), except for Non-commitment to safety. Significant differences were found between mothers and fathers in various FCRSS dimensions in the predicted direction, whereas no significant differences in FCRSS scores were found between young men and young women. As expected, associations were found between parents’ scores in various FCRSS dimensions and the reckless, angry, dissociative, anxious, and careful driving styles reported by the young drivers, as well as between young drivers’ FCRSS scores and their self-reported reckless, angry, dissociative, anxious, and careful driving styles

CCR5 deficiency predisposes to fatal outcome in influenza virus infection

Influenza epidemics affect all age groups, although children, the elderly and those with underlying medical conditions are the most severely affected. Whereas co-morbidities are present in 50% of fatal cases, 25-50% of deaths are in apparently healthy individuals. This suggests underlying genetic determinants that govern infection severity. Although some viral factors that contribute to influenza disease are known, the role of host genetic factors remains undetermined. Data for small cohorts of influenza-infected patients are contradictory regarding the potential role of chemokine receptor 5 deficiency (CCR5-Δ32 mutation, a 32 bp deletion in the CCR5 gene) in the outcome of influenza virus infection. We tested 171 respiratory samples from influenza patients (2009 pandemic) for CCR5-Δ32 and evaluated its correlation with patient mortality. CCR5-Δ32 patients (17.4%) showed a higher mortality rate than WT individuals (4.7%; P = 0.021), which indicates that CCR5-Δ32 patients are at higher risk than the normal population of a fatal outcome in influenza infection.This work was supported by the Instituto de Salud Carlos III (Programa especial de investigación sobre la gripe pandémica GR09/0040 and GR09/0023), Centro de Investigación Biomédica en Red en Enfermedades Respiratorias (CIBERES) and the Spanish Ministry of Science and Innovation (BFU 2011-26175)S

Pressure Mat Analysis of Walk and Trot Gait Characteristics in 66 Normal Small, Medium, Large, and Giant Breed Dogs

Objectives: To document temporospatial variables and gait symmetry measured by the GAITRite® system for normal, healthy dogs at the walk and trot with the leash side recorded.Study Design: Observational, prospective, cohort study.Sample Population: 66 healthy dogs of various common breeds with no evidence of lameness that were small (< 10 kg), medium (10- < 25 kg), large (25- < 40 kg), or giant (≥40 kg).Methods: Dogs walked and trotted at their preferred velocity on a pressure sensing walkway system. Video observation confirmed inclusion criteria were met for three valid trials at each gait for each dog. Coefficients of variance were used to summarize the data for analysis. Fore and hindlimb ratios were compared. Gait symmetry was assessed with the leash on the left and right side.Results: Coefficients of variation for gait parameters ranged from 20 to 28% for all except velocity and hind reach. There was no statistically significant difference in differences in fore and hindlimb ratios for stance %, GLS, TPI, or step:stride ratio, across weight categories or between walk and trot. Less than 8% of normal dogs had a GLS score < 90 (indicating lameness). Leash side did influence gait symmetry, since GLS, TPI, and step:stride all had statistically significant differences in means between leash side, irrelevant of the weight category or gait.Conclusions and Clinical Relevance: This system allowed simple, reliable gait assessment and values reported may be considered normal reference ranges for temporospatial variables collected with this system within the weight ranges and gaits reported. Controlling leash side and patient size is recommended for therapeutic intervention studies

Spanish validation and factor structure of the birth satisfaction scale-revised (BSS-R).

Objective: To translate and validate a Spanish-language version of the Birth Satisfaction Scale-Revised (BSS-R) and describe key measurement properties.Design: A cross-sectional instrument validation design examining factor structure, validity and reliability.Setting: Three public hospital sites in Spain. Participants: 202 women who had given birth within the past four weeks provided complete questionnaire data for analysis. Measurements and findings: Measures included the Spanish version of the BSS-R (S-BSS-R) and the Perceived Stress Scale (PSS). The tri-dimensional measurement model of the BSS-R was found to offer a good fit to Spanish data. Known-groups discriminant validity was found to be excellent with women experiencing a non-intervention delivery having higher S-BSS-R scores (

RETRACTED ARTICLE: Age-dependent Increase in Desmosterol Restores DRM Formation and Membrane-related Functions in Cholesterol-free DHCR24−/− Mice

Cholesterol is a prominent modulator of the integrity and functional activity of physiological membranes and the most abundant sterol in the mammalian brain. DHCR24-knock-out mice lack cholesterol and accumulate desmosterol with age. Here we demonstrate that brain cholesterol deficiency in 3-week-old DHCR24−/− mice was associated with altered membrane composition including disrupted detergent-resistant membrane domain (DRM) structure. Furthermore, membrane-related functions differed extensively in the brains of these mice, resulting in lower plasmin activity, decreased β-secretase activity and diminished Aβ generation. Age-dependent accumulation and integration of desmosterol in brain membranes of 16-week-old DHCR24−/− mice led to the formation of desmosterol-containing DRMs and rescued the observed membrane-related functional deficits. Our data provide evidence that an alternate sterol, desmosterol, can facilitate processes that are normally cholesterol-dependent including formation of DRMs from mouse brain extracts, membrane receptor ligand binding and activation, and regulation of membrane protein proteolytic activity. These data indicate that desmosterol can replace cholesterol in membrane-related functions in the DHCR24−/− mouse

Enhancing physicians’ radiology diagnostics of COVID-19’s effects on lung health by leveraging artificial intelligence

Introduction: This study aimed to develop an individualized artificial intelligence model to help radiologists assess the severity of COVID-19's effects on patients' lung health.Methods: Data was collected from medical records of 1103 patients diagnosed with COVID-19 using RT- qPCR between March and June 2020, in Hospital Madrid-Group (HM-Group, Spain). By using Convolutional Neural Networks, we determine the effects of COVID-19 in terms of lung area, opacities, and pulmonary air density. We then combine these variables with age and sex in a regression model to assess the severity of these conditions with respect to fatality risk (death or ICU).Results: Our model can predict high effect with an AUC of 0.736. Finally, we compare the performance of the model with respect to six physicians' diagnosis, and test for improvements on physicians' performance when using the prediction algorithm.Discussion: We find that the algorithm outperforms physicians (39.5% less error), and thus, physicians can significantly benefit from the information provided by the algorithm by reducing error by almost 30%

Fracción de carbono en la biomasa de Prosopis affinis sprengel (Fabaceae) en un bosque nativo del espinal (Argentina)

La estimación del carbono (C) almacenado en la biomasa forestal requiere información precisa acerca de la fracción de C por especie y por componente de la biomasa. Nuestro objetivo fue estimar la fracción de C en los diferentes componentes aéreos de la biomasa del ñandubay Prosopis affinis, y su variación por clase diamétrica en un bosque nativo del Espinal (Entre Ríos, Argentina). En 30 individuos de distintas clases diamétricas diferentes, se estimó la fracción de C en tres componentes: fustes (F), ramas grandes (RG), y ramas pequeñas+hojas+flores+frutos (RPHFF). Las fracciones medias de C evidenciaron diferencias altamente significativas (p<0,01) entre componentes, correspondiendo el menor valor a RPHFF (0,457). El mayor contenido de C correspondió a F, los cuales no difirieron estadísticamente de las RG (0,485 y 0,482, respectivamente). El valor medio de la fracción de C fue de 0,474 ± 0,023. Considerando la distribución de la biomasa individual en los diferentes componentes, y la fracción de C de cada uno, se obtuvo una fracción promedio ponderada de 0,472 ±0,013. Las fracciones de C mostraron diferencias significativas entre las diferentes clases diamétricas (p<0,05) solamente en los F, correspondiendo los mayores valores a los individuos con diámetros superiores a 20 cm. Los valores obtenidos en este trabajo resultan inferiores al valor por defecto de 0,50 sugerido por el IPCC (Intergovernmental Panel on Climate Change). La información generada constituye una herramienta que aporta precisión a las estimaciones del C almacenado y de las emisiones de CO2 causadas por la deforestación y degradación de los bosques nativos del espinal entrerriano.Estimation of carbon (C) stored in forest biomass requires accurate information of the C fraction for species and components of tree biomass. Our aim was to determine the C fraction in the biomass components of the ñandubay tree (Prosopis affinis), and its variation by diameter class, in a native forest of Espinal (Entre Ríos, Argentina). The C fraction was analyzed in the components of the above-ground biomass (trunks, T; large branches, LB; and small branches+leaves+flowers+fruits, SBLFF), on 30 individuals of five different diameter classes. There were highly significant differences (p<0,01) between the biomass components, and the lowest C fraction was found in SBLFFT (0,457). The component with the highest carbon concentration was T, although it did not differ significantly of the LB (0,485 and 0,482, respectively). The mean value of the C fraction was 0.474 ± 0,023. Considering the biomass partitioned by component, and the C fraction of each, the weighted average C fraction was 0,472 ± 0,013. The C fraction of T showed significant statistical differences (p<0,05) between diameter classes; the highest values were found in individuals with trunk diameters larger than 20 cm. The values obtained in this study are lower than the default value suggested by the Intergovernmental Panel on Climate Change (IPCC) (0,50). Information obtained is a tool that provides precision to the estimation of stored C and CO2 emissions from deforestation and degradation of the Espinal native forests of Entre Ríos province.EEA ParanáFil: Sione, Silvana María José. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Ledesma, Silvia Gabriela. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Rosenberger, Leandro Javier. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Oszust, José Daniel. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Carpp, Ignacio A. Universidad Nacional de Entre Ríos. Facultad de Ciencias Agropecuarias; ArgentinaFil: Wilson, Marcelo German. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná; Argentina.Fil: Andrade-Castañeda, Hernán J. Universidad del Tolima. Facultad de Ingeniería Agronómica; ColombiaFil: Sasal, Maria Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná; Argentin

Non-random distribution of deleterious mutations in the DNA and protein-binding domains of IRF6 are associated with Van Der Woude syndrome

Background: The development of the face occurs during the early days of intrauterine life by the formation of facial processes from the first Pharyngeal arch. Derangement in these well-organized fusion events results in Orofacial clefts (OFC). Van der Woude syndrome (VWS) is one of the most common causes of syndromic cleft lip and/or palate accounting for 2% of all cases. Mutations in the IRF6 gene account for 70% of cases with the majority of these mutations located in the DNA-binding (exon 3, 4) or protein-binding domains (exon 7-9). The current study was designed to update the list of IRF6 variants reported for VWS by compiling all the published mutations from 2013 to date as well as including the previously unreported VWS cases from Africa and Puerto Rico.Methods: We used PubMed with the search terms; "Van der Woude syndrome," "Popliteal pterygium syndrome," "IRF6," and "Orofacial cleft" to identify eligible studies. We compiled the CADD score for all the mutations to determine the percentage of deleterious variants.Results: Twenty-one new mutations were identified from nine papers. The majority of these mutations were in exon 4. Mutations in exon 3 and 4 had CADD scores between 20 and 30 and mutations in exon 7-9 had CADD scores between 30 and 40. The presence of higher CADD scores in the protein-binding domain (exon 7-9) further confirms the crucial role played by this domain in the function of IRF6. In the new cases, we identified five IRF6 mutations, three novel missense mutations (p.Phe36Tyr, p.Lys109Thr, and p.Gln438Leu), and two previously reported nonsense mutations (p.Ser424*and p.Arg250*).Conclusion: Mutations in the protein and DNA-binding domains of IRF6 ranked among the top 0.1% and 1% most deleterious genetic mutations, respectively. Overall, these findings expand the range of VWS mutations and are important for diagnostic and counseling purposes.</p