Nodal involvement evaluation in advanced cervical cancer: a single institutional experience

Purpose: To assess the usefulness of different imaging techniques in the detection of nodal involvement in patients with advanced cervical carcinoma. Moreover, to analyze the correlation between the presurgical (FIGO) and postsurgical (pTNM) staging classifications. Materials and Methods: All patients diagnosed with advanced cervical cancer (FIGO Stages IIB-IV) from 2005 to 2012 were selected. The medical charts of 51 patients that underwent presurgical assessment with posterior surgical staging by means of paraaortic lymphadenectomy, were reviewed. Nodal status assessment by computed tomography scan (CT scan), magnetic resonance imaging (MRI), positron emission tomography (PET), and sonography was compared, as well as the size given in imaging techniques compared to the final pathologic report information. Results: Presurgical analysis by CT scan, MRI, PET, and sonography showed pelvic nodal involvement in 51.3% of patients, and para-aortic involvement in 30.8% of cases. CT scan showed positive pelvic nodes in 35% of cases, but pathologic confirmation was observed in just 17.6% of cases. However, MRI resulted in higher rates of up to 48.8% of cases. Concerning para-aortic nodal involvement, CT scan showed positive nodes in 25% of cases, MRI in 3.2% of cases, and the pathologic report in 15.6% of cases. The authors found significant differences between staging groups among both classifications (FIGO vs. pTNM; p < 0.001). Eight cases (15.7%) were understaged by FIGO classification. Conclusions: Despite all imaging techniques available, none has demonstrated to be efficient enough to avoid the systematic study of para-aortic nodal status by means of surgical evaluatio

Bio-logic: gene expression and the laws of combinatorial logic

Original article can be found at: http://www.mitpressjournals.org/ Copyright MIT Press DOI: 10.1162/artl.2008.14.1.121At the heart of the development of fertilized eggs into fully formed organisms and the adaptation of cells to changed conditions are genetic regulatory networks (GRNs). In higher multi-cellular organisms, signal selection and multiplexing is performed at the cis-regulatory domains of genes, where combinations of transcription factors (TFs) regulate the rates at which the genes are transcribed into mRNA. To be able to act as activators or repressors of gene transcription, TFs must first bind to target sequences on the regulatory domains. Two TFs that act in concert may bind entirely independently of each other, but more often binding of the first one will alter the affinity of the other for its binding site. This paper presents a systematic investigation into the effect of TF binding dependencies on the predicted regulatory function of this “bio-logic”. Four extreme scenarios, commonly used to classify enzyme activation and inhibition patterns, for the binding of two TFs were explored: independent (the TFs bind without affecting each other’s affinities), competitive (the TFs compete for the same binding site), ordered (the TFs bind in a compulsory order), and joint binding (the TFs either bind as a preformed complex, or binding of one is virtually impossible in the absence of the other). The conclusions are: 1) the laws of combinatorial logic hold only for systems with independently binding TFs; 2) systems formed according to the other scenarios can mimic the functions of their Boolean logical counterparts, but cannot be combined or decomposed in the same way; and 3) the continuously scaled output of systems consisting of competitively binding activators and repressors can be more robustly controlled than that of single TF or (quasi-) logical multi-TF systems. Keywords: Transcription regulation, Genetic regulatory networks, Enzyme kinetics, Combinatorial logic, Non-Boolean continuous logic, Modelling.Peer reviewe

Random laser from engineered nanostructures obtained by surface tension driven lithography

The random laser emission from the functionalized thienyl-S,S-dioxide quinquethiophene (T5OCx) in confined patterns with different shapes is demonstrated. Functional patterning of the light emitter organic material in well defined features is obtained by spontaneous molecular self-assembly guided by surface tension driven (STD) lithography. Such controlled supramolecular nano-aggregates act as scattering centers allowing the fabrication of one-component organic lasers with no external resonator and with desired shape and efficiency. Atomic force microscopy shows that different geometric pattern with different supramolecular organization obtained by the lithographic process tailors the coherent emission properties by controlling the distribution and the size of the random scatterers

Experimental evidence of replica symmetry breaking in random lasers

Spin-glass theory is one of the leading paradigms of complex physics and describes condensed matter, neural networks and biological systems, ultracold atoms, random photonics, and many other research fields. According to this theory, identical systems under identical conditions may reach different states and provide different values for observable quantities. This effect is known as Replica Symmetry Breaking and is revealed by the shape of the probability distribution function of an order parameter named the Parisi overlap. However, a direct experimental evidence in any field of research is still missing. Here we investigate pulse-to-pulse fluctuations in random lasers, we introduce and measure the analogue of the Parisi overlap in independent experimental realizations of the same disordered sample, and we find that the distribution function yields evidence of a transition to a glassy light phase compatible with a replica symmetry breaking.Comment: 10 pages, 5 figure

Nucleosynthesis Predictions for Intermediate-Mass AGB Stars: Comparison to Observations of Type I Planetary Nebulae

Type I planetary nebulae (PNe) have high He/H and N/O ratios and are thought to be descendants of stars with initial masses of ~3-8Msun. These characteristics indicate that the progenitor stars experienced proton-capture nucleosynthesis at the base of the convective envelope, in addition to the slow neutron capture process operating in the He-shell (the s-process). We compare the predicted abundances of elements up to Sr from models of intermediate-mass asymptotic giant branch (AGB) stars to measured abundances in Type I PNe. In particular, we compare predictions and observations for the light trans-iron elements Se and Kr, in order to constrain convective mixing and the s-process in these stars. A partial mixing zone is included in selected models to explore the effect of a 13C pocket on the s-process yields. The solar-metallicity models produce enrichments of [(Se, Kr)/Fe] < 0.6, consistent with Galactic Type I PNe where the observed enhancements are typically < 0.3 dex, while lower metallicity models predict larger enrichments of C, N, Se, and Kr. O destruction occurs in the most massive models but it is not efficient enough to account for the > 0.3 dex O depletions observed in some Type I PNe. It is not possible to reach firm conclusions regarding the neutron source operating in massive AGB stars from Se and Kr abundances in Type I PNe; abundances for more s-process elements may help to distinguish between the two neutron sources. We predict that only the most massive models would evolve into Type I PNe, indicating that extra-mixing processes are active in lower-mass stars (3-4Msun), if these stars are to evolve into Type I PNe.Comment: 39 pages, accepted for publication in Ap

Frequency dynamics of gain-switched injection-locked semiconductor lasers

The frequency dynamics of gain-switched singlemode semiconductor lasers subject to optical injection is investigated. The requirements for low time jitter and reduced frequency chirp operation are studied as a function of the frequency mismatch between the master and slave lasers. Suppression of the power overshoot, typical during gain-switched operation, can be achieved for selected frequency detunings

Symmetries of Two Higgs Doublet Model and CP violation

We use the invariance of physical picture under a change of Lagrangian, the reparametrization invariance in the space of Lagrangians and its particular case -- the rephrasing invariance, for analysis of the two-Higgs-doublet extension of the SM. We found that some parameters of theory like tan beta are reparametrization dependent and therefore cannot be fundamental. We use the Z2-symmetry of the Lagrangian, which prevents a phi_1 phi_2 transitions, and the different levels of its violation, soft and hard, to describe a physical content of the model. In general, the broken Z2-symmetry allows for a CP violation in the physical Higgs sector. We argue that the 2HDM with a soft breaking of Z2-symmetry is a natural model in the description of EWSB. To simplify an analysis we choose among different forms of Lagrangian describing the same physical reality a specific one, in which the vacuum expectation values of both Higgs fields are real. A possible CP violation in the Higgs sector is described by using a two-step procedure with the first step identical to a diagonalization of mass matrix for CP-even fields in the CP conserved case. We find very simple necessary and sufficient condition for a CP violation in the Higgs sector. We determine the range of parameters for which CP violation and Flavor Changing Neutral Current effects are naturally small,what corresponds to a small dimensionless mass parameter nu= Re m_{12}^2/(2v1v2). We discuss how for small nu some Higgs bosons can be heavy, with mass up to about 0.6 TeV, without violating of the unitarity constraints. We discuss main features of the large nu case, which corresponds for nu -> infty to a decoupling of heavy Higgs bosons.Comment: 27 pages, extended discussion, references added, one figure, Revtex

Evolution and nucleosynthesis of helium-rich asymptotic giant branch models

There is now strong evidence that some stars have been born with He mass fractions as high as $Y \approx 0.40$ (e.g., in $\omega$ Centauri). However, the advanced evolution, chemical yields, and final fates of He-rich stars are largely unexplored. We investigate the consequences of He-enhancement on the evolution and nucleosynthesis of intermediate-mass asymptotic giant branch (AGB) models of 3, 4, 5, and 6 M$_\odot$ with a metallicity of $Z = 0.0006$ ([Fe/H] $\approx -1.4$). We compare models with He-enhanced compositions ($Y=0.30, 0.35, 0.40$) to those with primordial He ($Y=0.24$). We find that the minimum initial mass for C burning and super-AGB stars with CO(Ne) or ONe cores decreases from above our highest mass of 6 M$_\odot$ to $\sim$ 4-5 M$_\odot$ with $Y=0.40$. We also model the production of trans-Fe elements via the slow neutron-capture process (s-process). He-enhancement substantially reduces the third dredge-up efficiency and the stellar yields of s-process elements (e.g., 90% less Ba for 6 M$_\odot$, $Y=0.40$). An exception occurs for 3 M$_\odot$, where the near-doubling in the number of thermal pulses with $Y=0.40$ leads to $\sim$ 50% higher yields of Ba-peak elements and Pb if the $^{13}$C neutron source is included. However, the thinner intershell and increased temperatures at the base of the convective envelope with $Y=0.40$ probably inhibit the $^{13}$C neutron source at this mass. Future chemical evolution models with our yields might explain the evolution of s-process elements among He-rich stars in $\omega$ Centauri.Comment: 21 pages, 16 figures, accepted for publication by MNRAS. Stellar yields included as online data table