ESTUDIO DE ESTABILIDAD DE LA SUSPENSIÓN ORAL QUE CONTIENE SULFAMETOXAZOL Y TRIMETOPRIMA, EN DOSIS ADULTA Y PEDIÁTRICA DE DOS LABORATORIOS, AREQUIPA-PERÚ 2012-2013

ESTABILIDAD PROTOCOLO DE ESTUDIO DE ESTABILIDAD PREDICCIÓN DE LA VIDA ÚTIL EN CONDICIONES NATURALES DE ALMACENAMIENTO CONCEPTOS BÁSICOS CONDICIONES DE ALMACENAMIENTO LOTE INDUSTRIAL LOTE PILOTO INDUSTRIAL FECHA DE CADUCIDAD PERIODO DE VALIDEZ COMPROBADO PERIODO DE VALIDEZ TENTATIVO PRINCIPIO O INGREDIENTE ACTIVO ENVASE Y EMPAQUE PRIMARIO ENVASE Y EMPAQUE SECUNDARIO SUSPENSIONES .SUSPENSIONES ORALES APLICACIONES DE LAS SUSPENSIONES CARACTERÍSTICAS DE LAS SUSPENSIONES PREPARACIÓN DE UNA SUSPENSIÓN COMPONENTES DE UNA SUSPENSIÓN .SISTEMA ENVASE - CIERRE ESPECIFICACIONES ENVASES FARMACÉUTICOS CIERRES CAUSAS DE INESTABILIDAD DE LOS PRODUCTOS INCOMPATIBILIDAD OXIDO REDUCCIÓN HIDROLISIS DESCARBOXILACIÓN RACEMIZACIÓN REACCIONES FOTOQUÍMICAS AGREGADO DE EXCESO DE PRINCIPIO ACTIV

La dinámica de las inteligencias sociales en la era global. Una visión de hermenéutica analógica aplicada a dos contextos: México y España

A partir de la hermenéutica analógica de Mauricio Beuchot, y con base en la teoría de las inteligencias múltiples de Howard Gardner, se propone la interpretación de la sociedad global destacando los modelos de inteligencia que definen el rumbo de vida de los colectivos. La teoría de las inteligencias sociales ofrece una perspectiva de la globalización que contribuye a esclarecer el sentido de acontecer histórico socio-cultural del mundo actual. Las inteligencias sociales instauran capacidades y poderes simbólicos sobre los entornos e influyen de manera notable en las representaciones y relaciones cotidianas. En condiciones de globalidad algunas inteligencias sociales obtienen posición hegemónica debido a su efectividad y resultados, lo cual induce a individuos y grupos a desplazarse tácticamente hacia las inteligencias: estatista, científica, empresarial y delictiva

REX-001, a BM-MNC Enriched Solution, Induces Revascularization of Ischemic Tissues in a Murine Model of Chronic Limb-Threatening Ischemia

Background: Bone Marrow Mononuclear Cells (BM-MNC) constitute a promising alternative for the treatment of Chronic Limb-Threatening ischemia (CLTI), a disease characterized by extensive blockade of peripheral arteries, clinically presenting as excruciating pain at rest and ischemic ulcers which may lead to gangrene and amputation. BM-MNC implantation has shown to be efficient in promoting angiogenesis and ameliorating ischemic symptoms in CLTI patients. However, the variability seen between clinical trials makes necessary a further understanding of the mechanisms of action of BM-MNC, and moreover, to improve trial characteristics such as endpoints, inclusion/exclusion criteria or drug product compositions, in order to implement their use as stem-cell therapy. Materials: Herein, the effect of REX-001, a human-BM derived cell suspension enriched for mononuclear cells, granulocytes and CD34+ cells, has been assessed in a murine model of CLTI. In addition, a REX-001 placebo solution containing BM-derived red blood cells (BM-RBCs) was also tested. Thus, 24 h after double ligation of the femoral artery, REX-001 and placebo were administrated intramuscularly to Balb-c nude mice (n:51) and follow-up of ischemic symptoms (blood flow perfusion, motility, ulceration and necrosis) was carried out for 21 days. The number of vessels and vascular diameter sizes were measured within the ischemic tissues to evaluate neovascularization and arteriogenesis. Finally, several cell-tracking assays were performed to evaluate potential biodistribution of these cells. Results: REX-001 induced a significant recovery of blood flow by increasing vascular density within the ischemic limbs, with no cell translocation to other organs. Moreover, cell tracking assays confirmed a decrease in the number of infused cells after 2 weeks post-injection despite on-going revascularization, suggesting a paracrine mechanism of action. Conclusion: Overall, our data supported the role of REX-001 product to improve revascularization and ischemic reperfusion in CLTI

Molecular detection and parasite load of Trypanosoma cruzi in digestive tract tissue of Chagas disease patients affected by megacolon

Chagas disease, caused by the Trypanosoma cruzi parasite in the Americas affects similar to 7 million people, 30% with cardiac tissue damage and 10-15% with digestive disorders. In this study, we have developed a protocol to detect the presence of the parasite and estimate its load in resected dysfunctional tissue segments of chronically infected patients with digestive megacolon. We have included samples from 43 individuals, 38/5 with positive/negative serology for Chagas disease and digestive syndromes. Samples of 1.5 to 2.0 cm(2) were taken from different points of the dysfunctional digestive tract in specialized centres in Cochabamba, Bolivia. T. cruzi cultures were performed by inoculation with NNN-LIT culture medium, and genomic material was obtained from the samples for multiplex qPCR with TaqMan probes targeting satellite nuclear DNA. Cultures failed to isolate T. cruzi but qPCR reached a sensitivity of 42.1% (16/38) with all three spots and in triplicate. A new quantification methodology using synthetic satellite DNA as quantitation standard revealed parasite loads ranging from 2.2 x 10(2) to 1.0 x 10(6) satellite DNA copies/mu l. Positive samples from the distal end showed a higher parasite load. The results of the present study strengthen and add further evidence to previous findings in an experimental mouse model of chronic T. cruzi infection, providing a valuable tool to improve scientific knowledge on the relevance of the digestive tract in parasite persistence, and underline the need of a better understanding of host-pathogen interaction in digestive tissues, considering pathophysiology, disease immunology and response to treatment

FORMAÇÃO CORUMBATAÍ NA REGIÃO DE RIO CLARO/SP: PETROGRAFIA E IMPLICAÇÕES GENÉTICAS

A Formação Corumbataí na região de Rio Claro é representada predominantemente por siltitos argilosos com intercalações de siltitos arenosos, constituídos por illita, quartzo, feldspatos, carbonatos, hematita, montmorillonita, clorita e zeólita, de origem autígena e/ou detrítica. As variações texturais, e principalmente mineralógicas ao longo do empilhamento dos sedimentos da Formação Corumbataí, permitem dividi-la, nesta região, em cinco níveis mineralógico-texturais propostos nesse trabalho. As relações texturais e morfológicas entre componentes detríticos e autígenos sugerem transporte em ambiente árido, com área fonte de topografia suave, sem forte influência fluvial, gerando sedimentos que foram acumulados em um extenso mar epicontinental raso, com indícios de exposições aéreas já nos estratos basais, e com possível ligação com o oceano. Também evidencia que a espessura da lâmina d’água diminuiu progressivamente para o topo da sequência, ocorrendo oscilações em função da influência de marés e/ou tempestades. A composição mineralógica, em associação com a textura, evidencia que a Formação Corumbataí foi submetida a efeito termal após a litificação, promovendo transformações mineralógicas, hidrotermalismo e formação de brechas hidráulicas, provocadas pelo magmatismo básico que deu origem à Formação Serra Geral

GEOQUÍMICA E PROVENIÊNCIA DOS SEDIMENTOS DA FORMAÇÃO CORUMBATAÍ NA REGIÃO DE RIO CLARO/SP

A Formação Corumbataí na região de Rio Claro - Limeira - Araras é representada por uma sucessão de siltitos, constituídos principalmente por filossilicatos (predominando illita, embora também ocorram montmorillonita, biotita, clorita, caulinita e interestratificados regulares e irregulares, quartzo, plagioclásios e feldspatos potássicos, carbonatos, hematita, goethita e zeólitas, de origem autígena e/ou detrítica. Quimicamente as rochas da Formação Corumbataí são classificadas principalmente como wackes e secundariamente como folhelhos, cuja principal fonte são rochas ígneas félsicas e/ou rochas sedimentares quartzosas. A aplicação de índices de maturidade química nas rochas analisadas sugere que estas são quimicamente imaturas e que a fonte foi submetida a condições de intemperismo moderado. Diagramas discriminantes para ambientes tectônicos sugerem que a fonte dos sedimentos da Formação Corumbataí são rochas quimicamente semelhantes a rochas formadas em margem ativa e/ou arcos de ilha, embora depositados em outro contexto geotectônico (bacia intracratônica)

Atherosclerotic Pre-Conditioning Affects the Paracrine Role of Circulating Angiogenic Cells Ex-Vivo

In atherosclerosis, circulating angiogenic cells (CAC), also known as early endothelial progenitor cells (eEPC), are thought to participate mainly in a paracrine fashion by promoting the recruitment of other cell populations such as late EPC, or endothelial colony-forming cells (ECFC), to the injured areas. There, ECFC replace the damaged endothelium, promoting neovascularization. However, despite their regenerative role, the number and function of EPC are severely affected under pathological conditions, being essential to further understand how these cells react to such environments in order to implement their use in regenerative cell therapies. Herein, we evaluated the effect of direct incubation ex vivo of healthy CAC with the secretome of atherosclerotic arteries. By using a quantitative proteomics approach, 194 altered proteins were identified in the secretome of pre-conditioned CAC, many of them related to inhibition of angiogenesis (e.g., endostatin, thrombospondin-1, fibulins) and cell migration. Functional assays corroborated that healthy CAC released factors enhanced ECFC angiogenesis, but, after atherosclerotic pre-conditioning, the secretome of pre-stimulated CAC negatively affected ECFC migration, as well as their ability to form tubules on a basement membrane matrix assay. Overall, we have shown here, for the first time, the effect of atherosclerotic factors over the paracrine role of CAC ex vivo. The increased release of angiogenic inhibitors by CAC in response to atherosclerotic factors induced an angiogenic switch, by blocking ECFC ability to form tubules in response to pre-conditioned CAC. Thus, we confirmed here that the angiogenic role of CAC is highly affected by the atherosclerotic environment