Tissue specific requirement of Drosophila Rcd4 for centriole duplication and ciliogenesis

Rcd4 is a poorly characterized Drosophila centriole component whose mammalian counterpart, PPP1R35, is suggested to function in centriole elongation and conversion to centrosomes. Here, we show that rcd4 mutants exhibit fewer centrioles, aberrant mitoses, and reduced basal bodies in sensory organs. Rcd4 interacts with the C-terminal part of Ana3, which loads onto the procentriole during interphase, ahead of Rcd4 and before mitosis. Accordingly, depletion of Ana3 prevents Rcd4 recruitment but not vice versa. We find that neither Ana3 nor Rcd4 participates directly in the mitotic conversion of centrioles to centrosomes, but both are required to load Ana1, which is essential for such conversion. Whereas ana3 mutants are male sterile, reflecting a requirement for Ana3 for centriole development in the male germ line, rcd4 mutants are fertile and have male germ line centrioles of normal length. Thus, Rcd4 is essential in somatic cells but is not absolutely required in spermatogenesis, indicating tissue-specific roles in centriole and basal body formation

Tissue specific requirement of Drosophila Rcd4 for centriole duplication and ciliogenesis.

Rcd4 is a poorly characterized Drosophila centriole component whose mammalian counterpart, PPP1R35, is suggested to function in centriole elongation and conversion to centrosomes. Here, we show that rcd4 mutants exhibit fewer centrioles, aberrant mitoses, and reduced basal bodies in sensory organs. Rcd4 interacts with the C-terminal part of Ana3, which loads onto the procentriole during interphase, ahead of Rcd4 and before mitosis. Accordingly, depletion of Ana3 prevents Rcd4 recruitment but not vice versa. We find that neither Ana3 nor Rcd4 participates directly in the mitotic conversion of centrioles to centrosomes, but both are required to load Ana1, which is essential for such conversion. Whereas ana3 mutants are male sterile, reflecting a requirement for Ana3 for centriole development in the male germ line, rcd4 mutants are fertile and have male germ line centrioles of normal length. Thus, Rcd4 is essential in somatic cells but is not absolutely required in spermatogenesis, indicating tissue-specific roles in centriole and basal body formation

On the use of simulated experiments in designing tests for material characterization from full-field measurements

The present paper deals with the use of simulated experiments to improve the design of an actual mechanical test. The analysis focused on the identification of the orthotropic properties of composites using the unnotched Iosipescu test and a full-field optical technique, the grid method. The experimental test was reproduced numerically by finite element analysis and the recording of deformed grey level images by a CCD camera was simulated trying to take into account the most significant parameters that can play a role during an actual test, e.g. the noise, the failure of the specimen, the size of the grid printed on the surface, etc. The grid method then was applied to the generated synthetic images in order to extract the displacement and strain fields and the Virtual Fields Method was finally used to identify the material properties and a cost function was devised to evaluate the error in the identification. The developed procedure was used to study different features of the test such as the aspect ratio and the fibre orientation of the specimen, the use of smoothing functions in the strain reconstruction from noisy data, the influence of missing data on the identification. Four different composite materials were considered and, for each of them, a set of optimized design variables was found by minimization of the cost function

Lte1 contributes to Bfa1 localization rather than stimulating nucleotide exchange by Tem1

Reevaluation of Lte1’s involvement in the mitotic exit network reveals that it is involved in localizing Bfa1 to the spindle pole body but does not function as a GEF for Tem1

The midbody interactome reveals unexpected roles for PP1 phosphatases in cytokinesis

Abstract: The midbody is an organelle assembled at the intercellular bridge between the two daughter cells at the end of mitosis. It controls the final separation of the daughter cells and has been involved in cell fate, polarity, tissue organization, and cilium and lumen formation. Here, we report the characterization of the intricate midbody protein-protein interaction network (interactome), which identifies many previously unknown interactions and provides an extremely valuable resource for dissecting the multiple roles of the midbody. Initial analysis of this interactome revealed that PP1β-MYPT1 phosphatase regulates microtubule dynamics in late cytokinesis and de-phosphorylates the kinesin component MKLP1/KIF23 of the centralspindlin complex. This de-phosphorylation antagonizes Aurora B kinase to modify the functions and interactions of centralspindlin in late cytokinesis. Our findings expand the repertoire of PP1 functions during mitosis and indicate that spatiotemporal changes in the distribution of kinases and counteracting phosphatases finely tune the activity of cytokinesis proteins

Budding yeast ATM/ATR control meiotic double-strand break (DSB) levels by down-regulating Rec114, an essential component of the DSB-machinery

An essential feature of meiosis is Spo11 catalysis of programmed DNA double strand breaks (DSBs). Evidence suggests that the number of DSBs generated per meiosis is genetically determined and that this ability to maintain a pre-determined DSB level, or "DSB homeostasis", might be a property of the meiotic program. Here, we present direct evidence that Rec114, an evolutionarily conserved essential component of the meiotic DSB-machinery, interacts with DSB hotspot DNA, and that Tel1 and Mec1, the budding yeast ATM and ATR, respectively, down-regulate Rec114 upon meiotic DSB formation through phosphorylation. Mimicking constitutive phosphorylation reduces the interaction between Rec114 and DSB hotspot DNA, resulting in a reduction and/or delay in DSB formation. Conversely, a non-phosphorylatable rec114 allele confers a genome-wide increase in both DSB levels and in the interaction between Rec114 and the DSB hotspot DNA. These observations strongly suggest that Tel1 and/or Mec1 phosphorylation of Rec114 following Spo11 catalysis down-regulates DSB formation by limiting the interaction between Rec114 and DSB hotspots. We also present evidence that Ndt80, a meiosis specific transcription factor, contributes to Rec114 degradation, consistent with its requirement for complete cessation of DSB formation. Loss of Rec114 foci from chromatin is associated with homolog synapsis but independent of Ndt80 or Tel1/Mec1 phosphorylation. Taken together, we present evidence for three independent ways of regulating Rec114 activity, which likely contribute to meiotic DSBs-homeostasis in maintaining genetically determined levels of breaks

The Cdc14 Phosphatase Controls Resolution of Recombination Intermediates and Crossover Formation during Meiosis.

Meiotic defects derived from incorrect DNA repair during gametogenesis can lead to mutations, aneuploidies and infertility. The coordinated resolution of meiotic recombination intermediates is required for crossover formation, ultimately necessary for the accurate completion of both rounds of chromosome segregation. Numerous master kinases orchestrate the correct assembly and activity of the repair machinery. Although much less is known, the reversal of phosphorylation events in meiosis must also be key to coordinate the timing and functionality of repair enzymes. Cdc14 is a crucial phosphatase required for the dephosphorylation of multiple CDK1 targets in many eukaryotes. Mutations that inactivate this phosphatase lead to meiotic failure, but until now it was unknown if Cdc14 plays a direct role in meiotic recombination. Here, we show that the elimination of Cdc14 leads to severe defects in the processing and resolution of recombination intermediates, causing a drastic depletion in crossovers when other repair pathways are compromised. We also show that Cdc14 is required for the correct activity and localization of the Holliday Junction resolvase Yen1/GEN1. We reveal that Cdc14 regulates Yen1 activity from meiosis I onwards, and this function is essential for crossover resolution in the absence of other repair pathways. We also demonstrate that Cdc14 and Yen1 are required to safeguard sister chromatid segregation during the second meiotic division, a late action that is independent of the earlier role in crossover formation. Thus, this work uncovers previously undescribed functions of the evolutionary conserved Cdc14 phosphatase in the regulation of meiotic recombination

PROCESSING OF MESSAGES EXCHANGED ON A SOCIAL NETWORK

Lo strumento permette di confrontare sfaccettature che risulterebbero altrimenti di difficile analisi, differenziandosi da altri esistenti, in quanto non viene evidenziata un’univoca relazione tra i componenti, ma questi vanno a creare un sistema complesso, piacevole ed attrattivo dal punto di vista visuale, senza perdere di comprensibilità. Le attuali soluzioni presenti sul mercato non offrono la possibilità di visionare in modo olistico tutti i dati raccolti dai social network, bensì tali raccolte e aggregazioni portano solamente ad analisi di tipo unilaterale e reportistico, offrendo una navigazione talvolta esplorativa ma difficilmente di indagine. L’invenzione proposta offre la possibilità di raccogliere, organizzare e aggregare i dati in una modalità innovativa, coniugando una semplicità di ricerca e una completezza nella rappresentazione delle informazioni estratte, consentendo una maggiore analisi e interpretazione del fenomeno indagato

Data Design for Wellness and Sustainability

The paper describes the design-centered methodology and the design guidelines that guided the research and the development phase within the collaboration between the Architecture and Design Department of the Polytechnic of Turin and the Research Lab of TIM. The research wants to investigate how to expand boundaries of the wearable devices through the work of an interdisciplinary team and following the systemic design approach, a methodology that focuses on relations. Starting from an holistic diagnosis and the analysis of trends, the team defines six personas, useful to write down essential guidelines and the two new concept. According to behaviours, habits and requirements, these concepts put human at the center of the whole process and the design gives a particular attention to the sustainability. In this perspective sustainability is defined as the creation of positive relations among people, able to generate and encourage the development of well-being conditions with results on the community as a whole