Extracellular Vesicle-Associated Transitory Cell Wall Components and Their Impact on the Interaction of Fungi with Host Cells

Submitted by Fabricia Pimenta ([email protected]) on 2018-06-29T18:34:23Z No. of bitstreams: 1 ve_Marcio_Rodrigues_etal_CDTS_2016.pdf: 690221 bytes, checksum: a96164d483123b78f71bffabda9ffa1b (MD5)Approved for entry into archive by Fabricia Pimenta ([email protected]) on 2019-01-11T18:29:02Z (GMT) No. of bitstreams: 1 ve_Marcio_Rodrigues_etal_CDTS_2016.pdf: 690221 bytes, checksum: a96164d483123b78f71bffabda9ffa1b (MD5)Made available in DSpace on 2019-01-11T18:29:02Z (GMT). No. of bitstreams: 1 ve_Marcio_Rodrigues_etal_CDTS_2016.pdf: 690221 bytes, checksum: a96164d483123b78f71bffabda9ffa1b (MD5) Previous issue date: 2016-07-08Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro, RJ, Brazil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro, RJ, Brazil.Stony Brook University. Department of Molecular Genetics and Microbiology. Stony Brook, NY, USA / Veterans Administration Medical Center. Northport, NY, USA.Albert Einstein College of Medicine. Department of Microbiology and Immunology and Medicine. Bronx, NY, USA.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro, RJ, Brazil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Góes. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro, RJ, Brazil.Classic cell wall components of fungi comprise the polysaccharides glucans and chitin, in association with glycoproteins and pigments. During the last decade, however, system biology approaches clearly demonstrated that the composition of fungal cell walls include atypical molecules historically associated with intracellular or membrane locations. Elucidation of mechanisms by which many fungal molecules are exported to the extracellular space suggested that these atypical components are transitorily located to the cell wall. The presence of extracellular vesicles (EVs) at the fungal cell wall and in culture supernatants of distinct pathogenic species suggested a highly functional mechanism of molecular export in these organisms. Thus, the passage of EVs through fungal cell walls suggests remarkable molecular diversity and, consequently, a potentially variable influence on the host antifungal response. On the basis of information derived from the proteomic characterization of fungal EVs from the yeasts Cryptoccocus neoformans and Candida albicans and the dimorphic fungi Histoplasma capsulatum and Paracoccidioides brasiliensis, our manuscript is focused on the clear view that the fungal cell wall is much more complex than previously thought

Alpinia

Species of the genus Alpinia are widely used by the population and have many described biological activities, including activity against insects. In this paper, we describe the bioactivity of the essential oil of two species of Alpinia genus, A. zerumbet and A. vittata, against Rhodnius nasutus, a vector of Chagas disease. The essential oils of these two species were obtained by hydrodistillation and analyzed by GC-MS. The main constituent of A. zerumbet essential oil (OLALPZER) was terpinen-4-ol, which represented 19.7% of the total components identified. In the essential oil of A. vittata (OLALPVIT) the monoterpene β-pinene (35.3%) was the main constituent. The essential oils and their main constituents were topically applied on R. nasutus fifth-instar nymphs. In the first 10 min of application, OLALPVIT and OLALPZER at 125 μg/mL provoked 73.3% and 83.3% of mortality, respectively. Terpinen-4-ol at 25 μg/mL and β-pinene at 44 μg/mL provoked 100% of mortality. The monitoring of resistant insects showed that both essential oils exhibited antifeedant activity. These results suggest the potential use of A. zerumbet and A. vittata essential oils and their major constituents to control R. nasutus population

A espiritualidade conjugal segundo o pensamento do Pe. Henri Caffarel

A espiritualidade conjugal como caminho de santificação dos casais cristãos foi a pedra angular do pensamento do sacerdote francês Henri Caffarel ao conceber o Movimento das Equipes de Nossa Senhora (ENS). Tinha como objetivo solidificar a relação entre os cônjuges e seus familiares e contribuir com os casais para a busca da santidade conjugal. O desejo inspirador consistiu em proporcionar ao casal a possibilidade de converter a sua vida matrimonial, num verdadeiro estilo cristão de viver este sacramento no cotidiano, integrando a espiritualidade às tarefas humanas e da vida de igreja. A proposta, aos casais unidos pelo matrimônio, foi de vivenciarem uma sexualidade casta, agradável a Deus, sem, contudo, afastarem a nobreza do prazer do ato conjugal. Esposo e esposa, pela espiritualidade conjugal e com auxílio de instrumentos eficazes, seriam capacitados para gerar um itinerário do amor, da felicidade e da santidade em suas vidas, tendo na abnegação o fio condutor desta caminhada

Eggplant crop in no tillage system under different irrigation depths

Este estudo foi conduzido no município de Seropédica, RJ, com o objetivo de se determinar, em cultivo orgânico e sistema de plantio direto, a produtividade da cultura da berinjela sob diferentes lâminas de irrigação e sistemas de cultivo (consorciada com leguminosa e solteira). O delineamento experimental adotado foi o de blocos ao acaso, no esquema de parcela subdividida com quatro repetições caracterizando, na parcela, os tratamentos equivalentes à lâmina de irrigação (40; 70; 100 e 120% ETc) e, na subparcela, os sistemas de cultivo consorciado com feijão caupi e solteiro. Ambos os sistemas de cultivo não influenciaram a produtividade final da berinjela; no entanto, considerando as diferentes lâminas, a maior produtividade comercial foi de 65,41 Mg ha-1, obtida para uma lâmina total de 690,04 mm (106,8% ETc). A menor lâmina aplicada resultou em qualidade inferior dos frutos em relação às maiores lâminas, sendo a taxa de descarte dos frutos de 3 e 14%, respectivamente, para a maior e a menor lâmina.This study was carried out in the municipality of Seropedica-RJ, in order to determine, under organic farming and no tillage system, the yield of eggplants under different irrigation depths and cropping systems (intercropped with legumes and alone). The experimental design was in randomized blocks in a split plot design with four replications. The plots were characterized by the treatments corresponding to different water depths (40, 70, 100, 120% ETc), and the sub plots, the intercropping systems with cowpea and eggplant alone. Cropping systems did not influence the final yield of eggplant. However, considering the different irrigation depths, the highest commercial yield (65.41 Mg ha(-1)) was obtained for a total depth of 690.04 mm (106.8% ETc). The lower applied depth provided lower quality of fruit compared with that observed in the higher depths. The rate of fruit discard was 3 and 14%, respectively, for the higher and lower depths of irrigation

Avaliação de risco em mioloma múltiplo: resultados preliminares do grupo brasileiro de estudos de mieloma

The Durie/Salmon staging system continues to be used worldwide in patients with multiple myeloma. However, in recent years, new systems have been proposed. The International Myeloma Working Group performed a retrospective study with 11,179 patients and proposed an "International Staging System" utilizing serum levels of â2 microglobulin and albumin. In addition, current research has focused on the usefulness of cytogenetic and molecular data as prognostic factors. These data suggest that these parameters are powerful discriminators of a poor prognostic group of myeloma patients. Indeed, these prognostic indexes have been utilized in clinical trials, with interesting and encouraging results.O esquema de Durie / Salmon continua a ser utilizado para estadiar os pacientes com mieloma múltiplo. Recentemente, um novo sistema mais simples e eficaz foi proposto. O "International Myeloma Working Group" realizou um estudo retrospectivo com 11.179 pacientes e a partir destes dados propôs a criação de um "International Staging System (ISS)" utilizando os níveis séricos de ß2 microglobulina e de albumina ao diagnóstico. Além do ISS a pesquisa está voltada para identificar alterações citogenéticas e moleculares que se correlacionem com o prognóstico no mieloma múltiplo. Estes fatores prognósticos têm sido utilizados para estratificar pacientes em ensaios clínicos com resultados promissores

Avaliação de risco em mioloma múltiplo: resultados preliminares do grupo brasileiro de estudos de mieloma

The Durie/Salmon staging system continues to be used worldwide in patients with multiple myeloma. However, in recent years, new systems have been proposed. The International Myeloma Working Group performed a retrospective study with 11,179 patients and proposed an "International Staging System" utilizing serum levels of â2 microglobulin and albumin. In addition, current research has focused on the usefulness of cytogenetic and molecular data as prognostic factors. These data suggest that these parameters are powerful discriminators of a poor prognostic group of myeloma patients. Indeed, these prognostic indexes have been utilized in clinical trials, with interesting and encouraging results.O esquema de Durie / Salmon continua a ser utilizado para estadiar os pacientes com mieloma múltiplo. Recentemente, um novo sistema mais simples e eficaz foi proposto. O "International Myeloma Working Group" realizou um estudo retrospectivo com 11.179 pacientes e a partir destes dados propôs a criação de um "International Staging System (ISS)" utilizando os níveis séricos de ß2 microglobulina e de albumina ao diagnóstico. Além do ISS a pesquisa está voltada para identificar alterações citogenéticas e moleculares que se correlacionem com o prognóstico no mieloma múltiplo. Estes fatores prognósticos têm sido utilizados para estratificar pacientes em ensaios clínicos com resultados promissores.30suppl 26

The Chemical Profile of Senna velutina Leaves and Their Antioxidant and Cytotoxic Effects

Natural products can be a source of biomolecules with antioxidant activity which are able to prevent oxidative stress-induced diseases and show antitumor activity, making them important sources of new anticancer drug prototypes. In this context, this study aimed to analyze the chemical composition of an ethanol extract of Senna velutina leaves and to assess its antioxidant and cytotoxic activities in leukemic cells. The antioxidant properties were evaluated using a DPPH free radical scavenging assay and by examining the extract's inhibition of AAPH-induced lipid peroxidation in human erythrocytes. Its cytotoxicity and possible mechanisms of action were assessed in Jurkat and K562 leukemic cell lines. The ethanol extract contained flavonoids, such as epigallocatechin, epicatechin, kaempferol heteroside, rutin, and dimeric and trimeric proanthocyanidin derivatives. The extract exhibited antioxidant activity by scavenging free radicals and antihemolytic action, and it decreased malondialdehyde content in human erythrocytes. Furthermore, the extract also induced leukemic cell death by activating intracellular calcium and caspase-3, decreasing mitochondrial membrane potential, and arresting the cell cycle in S and G2 phases. Hence, S. velutina leaf extract contains antioxidant and antileukemic biomolecules with potential applications in diseases associated with oxidative stress and in the inhibition of tumor cell proliferation.This work was supported by grants of the Fundac¸˜ao de Apoio ao Desenvolvimento do Ensino, Ciˆencia e Tecnologia do Mato Grosso do Sul (FUNDECT), Conselho Nacional de Desenvolvimento Cient´ıfico e Tecnol´ogico (CNPq), and Coordenac¸˜ao de Aperfeic¸oamento de Pessoal de N´ıvel Superior (CAPES). Edson Lucas dos Santos, Carlos Alexandre Carollo, and Edgar J. Paredes-Gamero are recipients of fellowships from Brazilian National Research Council (CNPq), Brazil.info:eu-repo/semantics/publishedVersio

Avaliação de risco em mioloma múltiplo: resultados preliminares do grupo brasileiro de estudos de mieloma

The Durie/Salmon staging system continues to be used worldwide in patients with multiple myeloma. However, in recent years, new systems have been proposed. The International Myeloma Working Group performed a retrospective study with 11,179 patients and proposed an "International Staging System" utilizing serum levels of â2 microglobulin and albumin. In addition, current research has focused on the usefulness of cytogenetic and molecular data as prognostic factors. These data suggest that these parameters are powerful discriminators of a poor prognostic group of myeloma patients. Indeed, these prognostic indexes have been utilized in clinical trials, with interesting and encouraging results30269sem informaçãoO esquema de Durie / Salmon continua a ser utilizado para estadiar os pacientes com mieloma múltiplo. Recentemente, um novo sistema mais simples e eficaz foi proposto. O "International Myeloma Working Group" realizou um estudo retrospectivo com 11.179 pacientes e a partir destes dados propôs a criação de um "International Staging System (ISS)" utilizando os níveis séricos de ß2 microglobulina e de albumina ao diagnóstico. Além do ISS a pesquisa está voltada para identificar alterações citogenéticas e moleculares que se correlacionem com o prognóstico no mieloma múltiplo. Estes fatores prognósticos têm sido utilizados para estratificar pacientes em ensaios clínicos com resultados promissoressem informaçã