Microfluorìmetro Simplificado Para Estudo De Cálcio Intracelular

Compreendendo um sistema óptico, que é dividido em três subsistemas, sendo o primeiro identificado como subsistema de excitação; o segundo como subsistema de monitoração e o terceiro como subsistema óptico de detecção.BR9705280 (A)G01N21/17G01N33/48G01N21/17G01N33/48BR19979705280G01N21/17G01N33/48G01N21/17G01N33/4

Osseous oral hyaline ring granuloma mimicking a mandible tumor in a child with congenital agenesis of the corpus callosum

Hyaline ring granuloma (HRG) of the oral cavity is an uncommon disorder considered to be a foreign-body reaction resulting from implantation of food vegetable particles. Microscopically, it is characterized by the presence of structures of hyaline rings in an inflamed fibrous tissue background, which contains multinucleated giant cells. We present the case of a 4-year-old boy diagnosed with a mandible osseous HRG, which showed clinical and tomographic aspects suggestive of an aggressive bone tumor. The patient underwent surgical exploration and histopathologic analysis showed fragments composed predominantly of widespread dense connective tissue with an acute and chronic inflammatory infiltrate containing multinucleated giant cells and scattered areas of eosinophilic material associated with hyaline rings, strongly suggestive of vegetable particles. The eosinophilic material was positive for periodic acid-Schiff (PAS) and resistant to diastase digestion. These features led to diagnosis of osseous HRG. Scanning electron microscopy (SEM) analysis was performed for illustrative purposes and the multiple structures resembling vegetable particles were characterized in more detail. Although rare, this case highlights the importance of the clinician’s awareness regarding the existence of an osseous counterpart of HRG

Refluxo laringofaringeo: associação com manifestações extraesofagicas / Laryngopharyngeal reflux: association with extraesophageal manifestations

O refluxo laringofaríngeo possui uma grande incidência na população mundial dentre as patologias digestivas. Ele decorre, de modo direto e indireto, do refluxo do conteúdo gástrico, gerando uma inflamação do trato digestivo superior, podendo ser considerado uma manifestação do RGE ou uma entidade diferente. Tal patologia se manifesta com diferentes apresentações, que podem ser inespecíficas, dificultando, pois, o seu diagnóstico, e incluem  sintomas típicos do sistema gastrointestinal e manifestações atípicas extra esofágicas. Nesse sentido, o paciente pode apresentar globus faríngeo, pigarro, excesso de muco na garganta, gotejamento pós nasal, otite média, síndrome da apneia obstrutiva do sono, laringite e faringite crônica, e rinossinusite crônica. Exames como radiografia de tórax ou tomografia, MCII, Phmetria-24 horas, endoscopia nasal, em associação à clínica do paciente são úteis para a confirmação do quadro. A associação de mudanças do estilo de vida e IBP representam a forma terapêutica mais recomendada, sendo que a intervenção precoce é capaz de reduzir as chances de possíveis complicações

Avaliação de carcaça bovina: uma revisão sobre o uso do ultrassom

Diversos métodos de avaliação de carcaça bovina vêm sendo utilizados para expressar características relacionadas à carcaça, à qualidade da carne e ao produto final de um determinado sistema de produção. Atualmente, o uso do ultrassom na técnica de ultrassonografia é um desses métodos. Assim, o objetivo desta revisão é informar sobre o uso do ultrassom na avaliação de carcaça bovina, pontuar os principais indicadores de medidas na carcaça e sua avaliação, além de apresentar as vantagens e limitações do seu uso. A ultrassonografia vem sendo usada para quatro finalidades dentro das técnicas de avaliação de carcaças: avaliação da composição corporal quanto à deposição de músculo e gordura; avaliação dos reprodutores e sua progênie; seleção em rebanhos; e rendimento de carcaça para o mercado consumidor. A técnica permite a coleta de três principais medidas de carcaças in vivo: espessura de gordura subcutânea, área de olho de lombo e gordura de marmoreio, onde esses indicadores, feitos por ultrassom, apresentam boas correlações com as medidas de carcaça post mortem. Como benefícios da utilização da técnica tem-se a obtenção em tempo real, de forma rápida e barata, das características de composição de carcaça em animais ainda vivos. As principais limitações são tecnológicas, experiência do operador, e características particular do animal. Portanto, conclui-se que a ultrassonografia é uma ferramenta que tem sido largamente utilizada, sendo sua aplicação de extrema importância na avaliação da condição corporal e na seleção genética, possibilitando assim a produção de um animal que atenda o mercado consumidor

Micronutrient deficiencies and plasmodium vivax malaria among children in the Brazilian Amazon

Background: There is a growing body of evidence linking micronutrient deficiencies and malaria incidence arising mostly from P. falciparum endemic areas. We assessed the impact of micronutrient deficiencies on malaria incidence and vice versa in the Brazilian state of Amazonas. Methodology/Principal Findings: We evaluated children <10 years old living in rural communities in the state of Amazonas, Brazil, from May 2010 to May 2011. All children were assessed for sociodemographic, anthropometric and laboratory parameters, including vitamin A, beta-carotene, zinc and iron serum levels at the beginning of the study (May 2010) and one year later (May 2011). Children were followed in between using passive surveillance for detection of symptomatic malaria. Those living in the study area at the completion of the observation period were reassessed for micronutrient levels. Univariate Cox-proportional Hazards models were used to assess whether micronutrient deficiencies had an impact on time to first P. vivax malaria episode. We included 95 children median age 4.8 years (interquartile range [IQR]: 2.3-6.6), mostly males (60.0%) and with high maternal illiteracy (72.6%). Vitamin A deficiencies were found in 36% of children, beta-carotene deficiency in 63%, zinc deficiency in 61% and iron deficiency in 51%. Most children (80%) had at least one intestinal parasite. During follow-up, 16 cases of vivax malaria were diagnosed amongst 13 individuals. Micronutrient deficiencies were not associated with increased malaria incidence: vitamin A deficiency [Hazard ratio (HR): 1.51; P-value: 0.45]; beta-carotene [HR: 0.47; P-value: 0.19]; zinc [HR: 1.41; P-value: 0.57] and iron [HR: 2.31; P-value: 0.16]). Upon reevaluation, children with al least one episode of malaria did not present significant changes in micronutrient levels. Conclusion: Micronutrient serum levels were not associated with a higher malaria incidence nor the malaria episode influenced micronutrient levels. Future studies targeting larger populations to assess micronutrients levels in P. vivax endemic areas are warranted in order to validate these results. © 2016 Benzecry et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Spatial and temporal variations of diurnal ichthyofauna on surf-zone of São Francisco do Itabapoana beaches, Rio de Janeiro State, Brazil

Spatial and temporal variations of diurnal ichthyofauna and the environmental variables influences on its distribution were studied at the surf-zone of three beaches of São Francisco do Itabapoana, northern coast of Rio de Janeiro, Brazil. From August/1999 to August/2000, three beach seine hauls were made monthly, and environmental variables were recorded. A total number of 4,562 fishes (74,155g) were sampled at the three beaches, where estuarine-dependent species prevailed (44%), followed by marine (31%), estuarine (19%) and freshwater species (3%). Species richness, number of individuals and wet weight were significantly higher at Gargaú, followed by Manguinhos and Barra do Itabapoana, respectively. Canonical Correspondence Analysis highlighted influences of the rivers flushing, salinity and plant abundance on the diurnal ichthyofauna distribution and dynamics of São Francisco do Itabapoana surf-zone

Spatial and temporal variations of diurnal ichthyofauna on surf-zone of São Francisco do Itabapoana beaches, Rio de Janeiro State, Brazil

Spatial and temporal variations of diurnal ichthyofauna and the environmental variables influences on its distribution were studied at the surf-zone of three beaches of São Francisco do Itabapoana, northern coast of Rio de Janeiro, Brazil. From August/1999 to August/2000, three beach seine hauls were made monthly, and environmental variables were recorded. A total number of 4,562 fishes (74,155g) were sampled at the three beaches, where estuarine-dependent species prevailed (44%), followed by marine (31%), estuarine (19%) and freshwater species (3%). Species richness, number of individuals and wet weight were significantly higher at Gargaú, followed by Manguinhos and Barra do Itabapoana, respectively. Canonical Correspondence Analysis highlighted influences of the rivers flushing, salinity and plant abundance on the diurnal ichthyofauna distribution and dynamics of São Francisco do Itabapoana surf-zone