Variables that explain disordered eating behaviors among women: the mediating role of body dissatisfaction

Purpose To analyze the role of body dissatisfaction in the relationships of sociocultural influences, depression, and anxiety with disordered eating behaviors (DEB) in a sample of female Mexican university students. Methods A nonrandom sample of 526 female Mexican university students aged 18 to 25 years completed the Questionnaire of Influence on the Aesthetic Model of Body Shape (CIMEC-26), the Hospital Anxiety and Depression Scale (HADS), the Body Shape Questionnaire (BSQ-8D) and the Eating Attitudes Test (EAT-26). Results Through the mean model (χ2/df (5, n = 526) = 7.298, p = .199; NFI = .996; CFI = .999; RMSEA = .030; SRMR = .011), body dissatisfaction was found to mediate the relationships of influence of advertising, influence of social models and anxiety with DEB (restrictive dieting and bulimia). The variable with the most direct effect on restrictive dieting and bulimia was the influence of advertising. Body dissatisfaction partially mediated this relationship, as the influence of advertising had a significant direct effect on restrictive dieting and bulimia. The final model of direct and indirect effects explained 43% and 22% of the variance in restrictive dieting and bulimia, respectively. Conclusion The present study showed that body dissatisfaction partially mediated the relationships between influence of advertising, influence of social models, and anxiety with DEB among women. Thus, these variables should be taken into account in prevention and intervention programs targeting BED. Level V: Evidence obtained from a cross-sectional descriptive study. Level V Evidence obtained from a cross-sectional descriptive study.Funding for open access charge: Universidad Málaga/CBUA. This work was supported by the PRO-SNI program of the University of Guadalajara

Development of a glass-ceramic glaze formulated from industrial residues to improve the mechanical properties of the porcelain stoneware tiles

In this research a mixture of 90%wt of industrial residues (recycled soda-lime glass and ashes from a coalpower thermal plant) have been vitrified for their use as ‘‘secondary raw material”. Then, a glaze suspen-sion was prepared to be applied on the porcelain stoneware tile. The tested pieces have been fired by aconventional porcelain cycle at 1180 °C of maximum temperature. The XRD, XRF, SEM/EDS and thedilatometric analysis have been the instrumental techniques used to characterize the material. Finally,an ecological glass-ceramic glaze perfectly fitting on porcelain ceramic tile has been produced, exhibitinga unique phase, anorthite, which ensures a high flexural strength (around 96 MPa) and a significantVickers microhardness of 250 GPa, improving the mechanical properties of a conventional the porcelainceramic tile

Apoptosis of peritoneal leucocytes during early stages of Fasciola hepatica infections in sheep

Several immunomodulatory properties have been described in Fasciola hepatica infections. Apoptosis has been shown to be an effective mechanism to avoid the immune response in helminth infections. The aim of the present work was to study apoptosis in peritoneal leucocytes of sheep experimentally infected with F. hepatica during the early stages of infection. Five groups (n = 5) of sheep were used. Groups 2–5 were orally infected with 200 metacercariae (mc) and sacrificed at 1, 3, 9 and 18 days post-infection (dpi), respectively. Group 1 was used as the uninfected control (UC). Apoptosis was detected using three different methods 1) immunocytochemistry (ICC) with a polyclonal antibody anti-active caspase-3; 2) an annexin V flow cytometry assay using the Annexin V-FITC/propidium iodide (PI); and 3) transmission electron microscopy (TEM). The differential leucocyte count revealed that the majority of peritoneal granulocytes were eosinophils, which increased significantly at 9 and 18 dpi with respect to the uninfected controls. The ICC study revealed that the percentage of caspase-3+ apoptotic peritoneal leucocytes increased significantly from 3 dpi onwards with respect to the uninfected controls. The flow cytometry annexin V assay detected a very significant (P < 0.001) increase of apoptotic peritoneal macrophages, lymphocytes and granulocytes, which remained higher than in the UC until 18 dpi. Transmission electron microscopy studies also confirmed the presence of apoptosis in peritoneal eosinophils at 18 dpi. This is the first report of apoptosis induced by F. hepatica in the peritoneal leucocytes of sheep in vivo. The results of this work suggest the importance of apoptosis induction for the survival of the juvenile parasites in the peritoneal migratory stages of infection.This work was supported by EU grants (H2020-635408-PARAGONE) and the Spanish Ministry of Science grant AGL2015-67023-C2-1-R. The TEM studies were carried out by the Central Research Services (SCAI) of the University of CórdobaAccepted manuscriptVeterinari

A Partially Protective Vaccine for Fasciola hepatica Induced Degeneration of Adult Flukes Associated to a Severe Granulomatous Reaction in Sheep

Fasciolosis is an important economic disease of livestock. There is a global interest in the development of protective vaccines since current anthelmintic therapy is no longer sustainable. A better knowledge of the host-parasite interaction is needed for the design of effective vaccines. The present study evaluates the microscopical hepatic lesions in sheep immunized with a partially protective vaccine (VAC1), a non-protective vaccine (VAC2), and an infected control group (IC). The nature of granulomatous inflammation associated with degeneration of adult flukes found in the VAC1 group was characterized by immunohistochemistry. Hepatic lesions (fibrous perihepatitis, chronic tracts, bile duct hyperplasia, infiltration of eosinophils and lymphocytes and plasma cells) were significantly less severe in the VAC1 group than in the IC group. Dead adult flukes within bile ducts were observed only in the VAC1 group and were surrounded by a severe granulomatous inflammation composed by macrophages and multinucleate giant cells with a high expression of lysozyme, CD163 and S100 markers, and a low expression of CD68. Numerous CD3+ T lymphocytes and scarce infiltrate of FoxP3+ Treg and CD208+ dendritic cells were present. This is the first report describing degenerated flukes associated to a severe granulomatous inflammation in bile ducts in a F. hepatica vaccine trial

Effects of Thinning Intensity on Forest Floor and Soil Biochemical Properties in an Aleppo Pine Plantation after 13 Years: Quantity but Also Quality Matters

[EN] In order to quantify the impacts of silvicultural treatments in semiarid forests, it is necessary to know how they affect key aboveground processes and also properties characterizing the forest floor and mineral soil compartments. The general objective of this work is to study the mid-term effects of thinning intensity on forest floor and soil properties after 13 years following the intervention. The experimental design consisted of a randomized block design with four thinning treatments (3 thinning intensity plots plus a control or unmanaged plot) and three blocks or replicates. Several determinations, such as total organic carbon, dissolved organic carbon, or basal respiration, were performed for characterizing forest floor and mineral soil by considering three random sampling points per experimental plot. Thirteen years after thinning, total organic content, the different organic carbon fractions studied, and basal respiration were higher in the forest floor of the unmanaged plot. These results, however, were contrasted to those obtained for the mineral soil, where significant differences between the treatments were only observed in basal respiration and C/N ratio, while the different organic carbon fractions were not affected by thinning intensity. Our results suggest better soil quality where biological activity is enhanced as a consequence of improved environmental conditions and also litterfall input. The latter is especially important in forests with tree leaves of low biodegradability, where new understorey species promoted by thinning can provide higher nutrient availability for the remaining trees and, therefore, better forest resilience.A.J. Molina is the beneficiary of an "APOSTD' fellowship (APOSTD/2019/111) funded by the Generalitat Valenciana. The authors received national and international funding through the following projects: SILVADAPT.NET (RED2018-102719-T funded by MCIN/AEI/10.13039/501100011033), CEHYRFO-MED (CGL2017-86839-C3-2-R funded by MCIN/AEI/10.13039/501100011033 and FEDER a way to make Europe), and RESILIENTFORESTS (LIFE17 CCA/ES/000063)Molina, AJ.; Bautista, I.; Lull, C.; Campo García, ADD.; González Sanchis, MDC.; Lidón, A. (2022). Effects of Thinning Intensity on Forest Floor and Soil Biochemical Properties in an Aleppo Pine Plantation after 13 Years: Quantity but Also Quality Matters. Forests. 13(2):1-14. https://doi.org/10.3390/f1302025511413

Surface chemistry and germination improvement of Quinoa seeds subjected to plasma activation

Plasma treatment is recognized as a suitable technology to improve germination efficiency of numerous seeds. In this work Quinoa seeds have been subjected to air plasma treatments both at atmospheric and low pressure and improvements found in germination rate and percentage of success. Seed water uptake by exposure to water vapor, although slightly greater for plasma treated seeds, did not justify the observed germination improvement. To identify other possible factors contributing to germination, the chemical changes experienced by outer parts of the seed upon plasma exposure have been investigated by X-ray photoemission spectroscopy (XPS) and scanning electron microscopy (SEM-EDX). XPS revealed that the outer layers of the Quinoa plasma treated seeds were highly oxidized and appeared enriched in potassium ions and adsorbed nitrate species. Simultaneously, SEM-EDX showed that the enrichment in potassium and other mineral elements extended to the seed pericarp and closer zones. The disappearance from the surface of both potassium ions and nitrate species upon exposure of the plasma treated seeds to water vapor is proposed as a factor favoring germination. The use of XPS to study chemical changes at seed surfaces induced by plasma treatments is deemed very important to unravel the mechanisms contributing to germination improvement

Regeneration of ortho-chlorophenol-exhausted activated carbons with liquid water at high pressure and temperature

[EN]A study was undertaken of the regeneration of three activated carbons exhausted with ortho-chlorophenol. The regeneration process was carried out using liquid water at 623 K and 150 atm in the absence of oxygen. The efficiency of this procedure was analyzed by determining the rate and amount of ortho-chlorophenol adsorbed in successive adsorption–regeneration cycles. The present procedure showed a much greater efficiency than that reported for chemical and/or thermal regeneration. Effects of this regeneration on the adsorption kinetics, adsorption capacity and textural characteristics of the carbon were investigated. The increase in adsorption capacity of the regenerated carbon compared with that of the original carbon seems mainly due to the opening of porosity during the regeneration treatment

Fasciola hepatica induces Foxp3 T cell, proinflammatory and regulatory cytokine overexpression in liver from infected sheep during early stages of infection

The expression of T regulatory cells (Foxp3), regulatory (interleukin [IL]-10 and transforming growth factor beta [TGF-β]) and proinflammatory (tumor necrosis factor alpha [TNF-α] and interleukin [IL]-1β) cytokines was quantified using real time polymerase chain reaction (qRT-PCR) in the liver of sheep during early stages of infection with Fasciola hepatica (1, 3, 9, and 18 days post-infection [dpi]). Portal fibrosis was also evaluated by Masson’s trichrome stain as well as the number of Foxp3+ cells by immunohistochemistry. Animals were divided into three groups: (a) group 1 was immunized with recombinant cathepsin L1 from F. hepatica (FhCL1) in Montanide adjuvant and infected; (b) group 2 was uniquely infected with F. hepatica; and (c) group 3 was the control group, unimmunized and uninfected. An overexpression of regulatory cytokines of groups 1 and 2 was found in all time points tested in comparison with group 3, particularly at 18 dpi. A significant increase of the number of Foxp3+ lymphocytes in groups 1 and 2 was found at 9 and 18 dpi relative to group 3. A progressive increase in portal fibrosis was found in groups 1 and 2 in comparison with group 3. In this regard, group 1 showed smaller areas of fibrosis than group 2. There was a significant positive correlation between Foxp3 and IL-10 expression (by immunohistochemistry and qRT-PCR) just as between portal fibrosis and TGF-β gene expression. The expression of proinflammatory cytokines increased gradually during the experience. These findings suggest the induction of a regulatory phenotype by the parasite that would allow its survival at early stages of the disease when it is more vulnerable.This work was supported by EU Grant (H2020-635408-PARAGONE) and National grant (AGL2015-67023-C2-1-R). We thank Prof. John P. Dalton, Queen’s University Belfast, Northern Ireland, for providing recombinant FhCL1. Sequencing analysis and quality analysis of RNA were carried out by the Cen‑ tral Services for Research of the University of Córdoba (SCAI).Veterinari