Determinig the Specific Status of the Iberian Sturgeons by Means Genetic Analyses of Old Specimens

To clarify the species status of sturgeon from rivers of the Iberian Peninsula, eight molecular markers (4 nuclear and 4 mitochondrial) have been analysed in different specimens from historical museum samples and prehistoric samples from archaeological sites. These analyses indicate that one of these specimens (UGP captured in the Guadalquivir River in the 19th century) is A. sturio, based on all the eight molecular markers, four of them used from the first time in this study. In previous analyses based on 5 genetic markers, our group assigned two specimens captured in this river in the 1970-80s (EBD8173 and EBD8401) to the species A. naccarii, suggesting the presence of this species in the Iberian Peninsula. In this work, this conclusion is drawn after successfully obtaining a mitochondrial marker in a very old scute from a prehistoric site (Acinipo, about 1500 BC, from the Guadalquivir River basin). On the other hand, in the specimen EBD8174 captured in the Guadalquivir in 1975, we have obtained two new mitochondrial markers confirming that it can be considered A. sturio for all the mitochondrial markers, but nuclear ones identify it as A. naccarii. Finally, two very old samples (Nerja E-VI and Nerja N/62-63) were not successfully characterized by any molecular markers. Some aspects and consequences of our results are discussed, such as the origin of the “mosaic” specimen EBD8174 and, above all, the native status of A. naccarii in historic and prehistoric times in the southern Iberian Peninsula

Integration of Maps Enables a Cytogenomics Analysis of the Complete Karyotype in Solea senegalensis

The Pleuronectiformes order, which includes several commercially-important species, has undergone extensive chromosome evolution. One of these species is Solea senegalensis, a flatfish with 2n = 42 chromosomes. In this study, a cytogenomics approach and integration with previous maps was applied to characterize the karyotype of the species. Synteny analysis of S. senegalensis was carried out using two flatfish as a reference: Cynoglossus semilaevis and Scophthalmus maximus. Most S. senegalensis chromosomes (or chromosome arms for metacentrics and submetacentrics) showed a one-to-one macrosyntenic pattern with the other two species. In addition, we studied how repetitive sequences could have played a role in the evolution of S. senegalensis bi-armed (3, and 5-9) and acrocentric (11, 12 and 16) chromosomes, which showed the highest rearrangements compared with the reference species. A higher abundance of TEs (Transposable Elements) and other repeated elements was observed adjacent to telomeric regions on chromosomes 3, 7, 9 and 16. However, on chromosome 11, a greater abundance of DNA transposons was detected in interstitial BACs. This chromosome is syntenic with several chromosomes of the other two flatfish species, suggesting rearrangements during its evolution. A similar situation was also found on chromosome 16 (for microsatellites and low complexity sequences), but not for TEs (retroelements and DNA transposons). These differences in the distribution and abundance of repetitive elements in chromosomes that have undergone remodeling processes during the course of evolution also suggest a possible role for simple repeat sequences in rearranged regions

ALPHA-fitness test battery: health-related field-based fitness tests assessment in children and adolescents

Batería ALPHA-Fitness: test de campo para la evaluación de la condición física relacionada con la salud en niños y adolescentes (DOI:10.3305/nh.2011.26.6.5611)En el presente estudio describe el trabajo desarrollado para la creación de la batería ALPHA-Fitness de test de campo para la evaluación de la condición física relacionada con la salud en niños y adolescentes. La batería ALPHA-Fitness basada en la evidencia incluye los siguientes test: 1) test de ida y vuelta de 20 metros para evaluar la capacidad aeróbica, 2) test de fuerza de prensión manual y 3) test de salto de longitud a pies juntos para evaluar la capacidad músculo-esquelética, y 4) el IMC, 5) el perímetro de la cintura, y 6) los pliegues cutáneos (tríceps y subscapular) para evaluar la composición corporal. Además, se incluyen 2 variantes: i) batería ALPHA-Fitness de alta prioridad. Esta variante incluye todos los tests excepto la medida de pliegues cutáneos, y ii) la batería ALPHA-Fitness extendida, que incluye todos los test y además del test de velocidad y agilidad de 4 x 10 m.Hereby we summarize the work developed by the ALPHA (Assessing Levels of Physical Activity) Study and describe the tests included in the ALPHA health-related fitness test battery for children and adolescents. The evidence-based ALPHA-Fitness test battery include the following tests: 1) the 20 m shuttle run test to assess cardiorespiratory fitness; 2) the handgrip strength and 3) standing broad jump to assess musculoskeletal fitness, and 4) body mass index, 5) waist circumference; and 6) skinfold thickness (triceps and subscapular) to assess body composition. Furthermore, we include two versions: 1) the high priority ALPHA health-related fitness test battery, which comprises all the evidence-based fitness tests except the measurement of the skinfold thickness; and 2) the extended ALPHA health-related fitness tests battery for children and adolescents, which includes all the evidence-based fitness tests plus the 4 x 10 m shuttle run test to assess motor fitness.El estudio ALPHA ha sido financiado con fondos de la Unión Europea (Ref: 2006120), El Ministerio de Educación (EX-2008-0641, EX-2009-0899, AP2008-03806), los fondos FEDER (Acciones Complementarias DEP2007-29933-E), y el Ministerio de Ciencia e Innovación (RYC-2010-05957, CEB09-0005 GREIB, JCI-2010-07055), y la Swedish Lung-Heart Foundation (20090635)

Land- and water-based exercise intervention in women with fibromyalgia: the al-andalus physical activity randomised controlled trial

Background The al-Andalus physical activity intervention study is a randomised control trial to investigate the effectiveness of a land- and water-based exercise intervention for reducing the overall impact of fibromyalgia (primary outcome), and for improving tenderness and pain-related measures, body composition, functional capacity, physical activity and sedentary behaviour, fatigue, sleep quality, health-related quality of life, and cognitive function (secondary outcomes) in women with fibromyalgia. Methods/Design One hundred eighty women with fibromyalgia (age range: 35-65 years) will be recruited from local associations of fibromyalgia patients in Andalucía (Southern Spain). Patients will be randomly assigned to a usual care (control) group (n = 60), a water-based exercise intervention group (n = 60) or a land-based exercise intervention group (n = 60). Participants in the usual care group will receive general physical activity guidelines and participants allocated in the intervention groups will attend three non-consecutive training sessions (60 min each) per week during 24 weeks. Both exercise interventions will consist of aerobic, muscular strength and flexibility exercises. We will also study the effect of a detraining period (i.e., 12 weeks with no exercise intervention) on the studied variables. Discussion Our study attempts to reduce the impact of fibromyalgia and improve patients' health status by implementing two types of exercise interventions. Results from this study will help to assess the efficacy of exercise interventions for the treatment of fibromyalgia. If the interventions would be effective, this study will provide low-cost and feasible alternatives for health professionals in the management of fibromyalgia. Results from the al-Andalus physical activity intervention will help to better understand the potential of regular physical activity for improving the well-being of women with fibromyalgia.This study was supported by the Consejeria de Turismo, Comercio y Deporte (CTCD-201000019242-TRA), the Spanish Ministry of Science and Innovation (I + D + I DEP2010-15639, grants: BES-2009-013442, BES-2011-047133, RYC-2010-05957, RYC-2011-09011), the Swedish Heart-Lung Foundation (20090635), the Spanish Ministry of Education (AP-2009-3173), Granada Research of Excelence Initiative on Biohealth (GREIB), Campus BioTic, University of Granada, Spain and European University of Madrid. Escuela de Estudios Universitarios Real Madrid. 2010/04RM

El género "Erysimum" L. ("Cruciferae") en Andalucía (España)

Se realiza la revisión de las especies vivaces del género Erysimum L. en Andalucía y se discute la utilidad de los caracteres taxonómicos. Para cada taxon se da el nombre correcto, sinonimia, descripción, tipo, número cromosomático (se detectan tres nuevos niveles de ploidía en diferentes táxones), distribución, habitat y lista de material estudiado. Se propone una nueva combinación, E. medio-hispanicum Polatschek subsp. rondae (Polatschek) Blanca & Morales, y se describe un nuevo taxon, E. baeticum (Heywood) Polatschek subsp. bastetanum Blanca & Morales.A taxonomic revision of the genus Erysimum L. in Andalusia (Southern Iberian Peninsula) was carried out. We discuss the greater utility of the following taxonomic characters: shoot (size and branching), phyllotaxy, length of the raceme relative to the shoot in flower, flower size and color, siliquae (positíon relative to the axis, length of the style), and chromosome number. Three new ploidy levéis have been detected: 2n = 28 in E. popovii, In = 14 in E. medio- hispanicum subsp. medio-hispanicum, and 2n = 56 in E. baeticum subsp. baeticum. The results correct various ploidy levéis which have been erroneously ascribed to certain taxa, and indicate the presence of different ploidy levels within the same taxon. For each taxon the correct name, synonyms, type description, chromosome number, distribution, ecological habit, and a list of the material studied is given. We propose a new combination, E. mediohispanicum Polatschek subsp. rondae (Polatschek) Blanca & Morales, and a new taxon, E. baeticum (Heywood) Polatschek subsp. bastetanum Blanca & Morales