Nutritional characterization of Quinoa from brazilian variety BRs Piabiru / Caracterização nutricional da Quinoa da variedade brasileira BRs Piabiru

Quinoa stands out for its high resistance to adverse weather and soil conditions and, above all, for its high nutritional value. It surpasses most cereals in the amount of proteins, fats, fibers, vitamins and minerals, presenting a greater balance in the distribution of essential amino acids. In addition to containing high nutritional quality, quinoa is also characterized by being gluten-free, enabling a greater variety and offer of more nutritious food products suitable for people with celiac disease. Despite all these characteristics, the use and knowledge about quinoa is still little in Brazil due to the high cost of imported grain and the lack of knowledge of its benefits by the majority of the Brazilian population, with its industrialization and commercialization still very limited. The aim of this work was to evaluate nutritional quality through analysis of vitamins and carotenoids of the quinoa of the BRS Piabiru variety, developed and adapted to the conditions of the Brazilian savanna (Cerrado). Wholemeal quinoa flour showed high concentrations of carotenoids and vitamins, especially niacin and tocopherols, showing their potential nutritional value, especially when compared to the flours of other cereals commonly used in human consumption.

TGF-β1 and its association with clinicopathological features, proliferative activity and prognosis in oral squamous cell carcinoma: An immunohistochemical study

Introduction: The prognostic value of transforming growth factor beta-1 (TGF-ß1) in oral cancer remains unclear. Therefore, the aim of this study was to evaluate TGF-β1 expression in oral squamous cell carcinoma (OSCC) samples and its association with clinicopathological data, tumor proliferative activity and patients’ prognosis. Methods: Sixty-eight patients with histopathological diagnosis of OSCC were included, as 9 cases of normal oral mucosa for comparison purposes. OSCC sample was categorized according to patients’ outcomes in favorable prognosis (n=30) or unfavorable prognosis (n=38). Immunohistochemical staining for TGF-β1 and Ki-67 was performed. The slides were semi-quantitatively and quantitatively evaluated for TGF-β1 and Ki-67, respectively. Results: TGF-β1 was significantly increased in OSCC compared to normal oral mucosa (<0.01). An inverse correlation was found between TGF-β1 and Ki67 staining in OSCC (p=0.01). No association was found between TGF-β1 expression and OSCC clinicopathological features, prognosis or survival. Conclusion: TGF-β1 had no prognostic value and appears to maintain it’s suppressive role concerning cell proliferation.   Keywords: Head and neck neoplasms; prognosis; transforming growth factor

Dentinoma: apresentação de um caso

The authors present a case of dentinoma, place in the apical region between the left central and lateral incisors of the superior maxila in a 13 years old, male.Os autores apresentam um caso de dentinoma, com localização na porção anterior da maxila, junto ao ápice dos incisivos central e lateral esquerdo, em paciente do sexo masculino com 13 anos de idade

Dentinoma: apresentação de um caso

The authors present a case of dentinoma, place in the apical region between the left central and lateral incisors of the superior maxila in a 13 years old, male.Os autores apresentam um caso de dentinoma, com localização na porção anterior da maxila, junto ao ápice dos incisivos central e lateral esquerdo, em paciente do sexo masculino com 13 anos de idade

Agreement between clinical and histopathologic diagnoses and completeness of oral biopsy forms

Abstract The present study aimed to assess the rate of agreement between clinical and histopathological diagnoses and to report the frequency of completed forms for specimens that were subjected to histopathological examination and retrospectively examined. Data from 8,168 specimens submitted to histopathological examination were retrieved from the records. A total of 5,368 cases were included. Agreement was defined based on the definition of lesion nature according to its diagnostic category. Sensitivity, specificity, and positive and negative predictive values were calculated for each diagnostic category. The highest rate of agreement was observed for periapical lesions (92.6%), followed by potentially malignant disorders (90.1%) and non-neoplastic proliferative disorders (89.3%). Low rates of histopathological confirmation of the clinical impression were observed for mesenchymal tumors (25.0%) and cysts (44.2%). Sensitivity values were > 0.70 for all lesions, except for cysts (0.51). Specificity was relatively high, ranging from 0.97 to 1.00. The frequency of incomplete biopsy forms ranged from 16.8% (malignant tumors of oral mucosal epithelium) to 51.0% (nonspecific inflammatory reaction). The most frequently completed biopsy forms corresponded to epithelial malignant tumors (83.2%) and glandular inflammation (72.3%). In conclusion, there was an acceptable level of agreement. The low level of completeness of biopsy forms indicates little awareness about the relevance of gathering detailed information during clinical examination

TGF-β1 and its association with clinicopathological features, proliferative activity and prognosis in oral squamous cell carcinoma: An immunohistochemical study

Introduction: The prognostic value of transforming growth factor beta-1 (TGF-ß1) in oral cancer remains unclear. Therefore, the aim of this study was to evaluate TGF-β1 expression in oral squamous cell carcinoma (OSCC) samples and its association with clinicopathological data, tumor proliferative activity and patients’ prognosis.Methods: Sixty-eight patients with histopathological diagnosis of OSCC were included, as 9 cases of normal oral mucosa for comparison purposes. OSCC sample was categorized according to patients’ outcomes in favorable prognosis (n=30) or unfavorable prognosis (n=38). Immunohistochemical staining for TGF-β1 and Ki-67 was performed. The slides were semi-quantitatively and quantitatively evaluated for TGF-β1 and Ki-67, respectively.Results: TGF-β1 was significantly increased in OSCC compared to normal oral mucosa (&lt;0.01). An inverse correlation was found between TGF-β1 and Ki67 staining in OSCC (p=0.01). No association was found between TGF-β1 expression and OSCC clinicopathological features, prognosis or survival.Conclusion: TGF-β1 had no prognostic value and appears to maintain it’s suppressive role concerning cell proliferation. Keywords: Head and neck neoplasms; prognosis; transforming growth factors

Analysis of the Epithelium-Mesenchymal Transition Process on Oral Squamous Cell Carcinomas

<div><p>Abstract The aim of this study is to evaluate the immunohistochemical expression of E-cadherin, N-cadherin and Bmi-1, and their association with clinical parameters and with the degree of histopathological differentiation in oral squamous cell carcinomas. 65 squamous cell carcinoma samples were used for constructing a tissue microarray block, and then immunohistochemistry was performed for different markers. A semi-quantitative analysis of the amount of positive tumor cells was performed by two blind and calibrated observers (Kappa>0.75). The statistical Mann-Whitney and Kruskal-Wallis tests were used to evaluate the data. The correlation between variables was investigated by the Spearman test, and the significance level set at p<0.05. We observed higher expression of Bmi-1 in tumors located in the palate (p<0.0001). In addition, poorly differentiated tumors had a greater amount of Bmi-1 positive cells (p=0.0011). Regarding the other correlations between variables, no significant associations were detected. In conclusion, poorly differentiated squamous cell carcinomas located in the palate have higher immunostaining of Bmi-1, which can characterize activation of the Epithelial-Mesenchymal Transition process in these tumors.</p></div

Fibronectin induces smaller adhesion on low E-cadherin expression OSCC cell line.

<p>L^inv/H^E-cad (A) or H^inv/L^E-cad (B) invasive OSCC were plated on laminin or fibronectin, fixed and stained for E-cadherin and actin, paxillin, vinculin and FAK. White arrows indicate the signal of E-cadherin between cells. Scale bar = 20μm. Data regarding adhesion properties (C) were obtained using Total Internal Reflectance Fluorescent microscopy analysis of H^inv/L^E-cad OSCC cells expressing paxillin-GFP and plated on laminin (light gray) or fibronectin (dark gray). The data shows the assembly speed (μm/sec), adhesion area (μm²), total adhesion area (as % of total protrusion area) and adhesion length (μm). Results are expressed as mean ± SEM. (*) p = 0.05; (**) p < 0.01, according to Student T—test.</p