Cardo Silvestre como cultivo bioenergético

Cardo Silvestre como cultivo bioenergéticoFil: Mancini, Micaela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentin

Control capacity of the LPSc 1067 strain of Beauveria bassiana (Ascomycota: Hypocreales) on different species of grasshoppers (Orthoptera: Acrididae: Melanoplinae), agricultural pests in Argentina

Grasshoppers affect agriculture worldwide, causing serious economic damage. Currently, the application of chemical insecticides against grasshoppers is the only effective strategy, even considering the significant environmental concern. This study aimed to test the entomopathogenic fungi Beauveria bassiana (LPSc 1067) as biocontrol agent on six harmful grasshopper species in Argentina. Significant differences were observed (DF= 5; F= 9.93; P<0.0001) when considering B. bassiana pathogenicity on third-instar nymphs of the different grasshopper species. The highest mortality (100%) was registered on Trimerotropis pallidipennis and Dichroplus maculipennis nymphs while the lowest mortality (48.6 ±3.5%) was observed on Scotussa lemniscata nymphs. The lowest mean survival time (MST) was recorded for T. pallidipennis (3.5 ±0.15 days) and the highest MST was observed on Dichroplus pratensis nymphs (7.48 ±0.28 days). Results suggest that B. bassiana LPSc 1067 may constitute an excellent candidate to be further studied as biological control agent of T. pallidipennis and D. maculipennis. Highlights: Melanoplinae grasshoppers include several species considered plagues. Entomopathogenic fungi as biocontrol agents are considered excellent alternatives to chemical control. The efficacy of bassiana (LPSc 1067) on six grasshopper species of Argentina. The isolate LPSc 1067 could be considered a biological controller pallidipennis and D. maculipennis.Grasshoppers affect agriculture worldwide, causing serious economic damage. Currently, the application of chemical insecticides against grasshoppers is the only effective strategy, even considering the significant environmental concern. This study aimed to test the entomopathogenic fungi Beauveria bassiana (LPSc 1067) as biocontrol agent on six harmful grasshopper species in Argentina. Significant differences were observed (DF= 5; F= 9.93; P<0.0001) when considering B. bassiana pathogenicity on third-instar nymphs of the different grasshopper species. The highest mortality (100%) was registered on Trimerotropis pallidipennis and Dichroplus maculipennis nymphs while the lowest mortality (48.6 ±3.5%) was observed on Scotussa lemniscata nymphs. The lowest mean survival time (MST) was recorded for T. pallidipennis (3.5 ±0.15 days) and the highest MST was observed on Dichroplus pratensis nymphs (7.48 ±0.28 days). Results suggest that B. bassiana LPSc 1067 may constitute an excellent candidate to be further studied as biological control agent of T. pallidipennis and D. maculipennis. Highlights: Melanoplinae grasshoppers include several species considered plagues. Entomopathogenic fungi as biocontrol agents are considered excellent alternatives to chemical control. The efficacy of bassiana (LPSc 1067) on six grasshopper species of Argentina. The isolate LPSc 1067 could be considered a biological controller pallidipennis and D. maculipennis.

Potential use of cardoon oil for biodiesel production and its comparison with soybean oil

El cardo (silvestre y cultivado) es una especie con potencial para ser utilizado como cultivo energético con aprovechamiento integral para producción de biocombustible sólidos (pellets) y líquidos (bioetanol y biodiesel). Los objetivos del presente trabajo fueron estudiar el perfil de ácidos grasos del aceite de cardo mediante técnicas químicas específicas, y la comparación con el perfil de ácidos grasos del aceite de soja. Los resultados obtenidos demuestran que el aceite de cardo presenta características adecuadas para ser destinado a la producción de biodiesel lo que permitiría la incorporación de un nuevo cultivo alternativo al circuito productivo-comercial con fines energéticos.Cardoon (wild and cultivated) is a species with potential to be fully used as an energy crop for solid (pellets) and liquid (bioethanol and biodiesel) biofuels production. The objective of this work was to study the fatty acids profile of cardoon oil by specific chemical techniques and its comparison with the fatty acids profile of Soybean oil. The results show that cardoon oil has suitable characteristics to be used for biodiesel production which would allow the incorporation of a new alternative crop to production circuit for energy purposes.Fil: Morero, Ana Carina. Universidad del Centro Educativo Latinoamericano; ArgentinaFil: Gatti, Bernardita. Universidad del Centro Educativo Latinoamericano; ArgentinaFil: Malik, Yair. Universidad del Centro Educativo Latinoamericano; ArgentinaFil: Mancini, Micaela. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Rosario. Instituto de Investigaciones En Ciencias Agrarias de Rosario; ArgentinaFil: Lanza Volpe, Melisa. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; ArgentinaFil: Cravero, Vanina Pamela. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnol.conicet - Rosario. Instituto de Investigaciones En Ciencias Agrarias de Rosario; Argentin

Capacidad de control de la cepa LPSc 1067de Beauveria bassiana (Ascomycota: Hypocreales) sobre diferentes especies de tucuras (Orthoptera: Acrididae: Melanoplinae), plagas del agro de Argentina

Grasshoppers affect agriculture worldwide, causing serious economic damage. Currently, the application of chemical insecticides against grasshoppers is the only effective strategy, even considering the significant environmental concern. This study aimed to test the entomopathogenic fungi Beauveria bassiana (LPSc 1067) as biocontrol agent on six harmful grasshopper species in Argentina. Significant differences were observed (DF= 5; F= 9.93; P<0.0001) when considering B. bassiana pathogenicity on third-instar nymphs of the different grasshopper species. The highest mortality (100%) was registered on Trimerotropis pallidipennis and Dichroplus maculipennis nymphs while the lowest mortality (48.6 ±3.5%) was observed on Scotussa lemniscata nymphs. The lowest mean survival time (MST) was recorded for T. pallidipennis (3.5 ±0.15 days) and the highest MST was observed on Dichroplus pratensis nymphs (7.48 ±0.28 days). Results suggest that B. bassiana LPSc 1067 may constitute an excellent candidate to be further studied as biological control agent of T. pallidipennis and D. maculipennis.Las tucuras causan graves pérdidas económicas en la agricultura a nivel mundial. En la actualidad, los insecticidas químicos siguen siendo el único medio utilizado para el control de acridios, pero los efectos de su utilización son ambientalmente preocupantes. El objetivo de este trabajo fue probar la eficacia de la cepa Beauveria bassiana (LPSc 1067) sobre seis especies de tucuras consideradas plagas de Argentina. En cuanto a la patogenicidad de B. bassiana sobre ninfas de tercer estadio de las diferentes especies tratadas, se encontraron diferencias significativas (DF= 5; F= 9.93; P<0.0001). Los valores de mortalidad más altos (100%) se registraron en ninfas de Trimerotropis pallidipennis y Dichroplus maculipennis y la mortalidad más baja se observó en ninfas de Scotussa lemniscata con una mortalidad de 48,6 +3,5%. El tiempo medio de supervivencia (MST) más bajo se registró para T. pallidipennis (3,5 +0,15 días) y el MST más alto se observó en ninfas de Dichroplus pratensis (7,48 +0,28 días). Los resultados sugieren que B. bassiana LPSc 1067 constituye un excelente candidato para ser estudiado en profundidad como agente de control biológico de T. pallidipennis y D. maculipennis.Fil: Pelizza, Sebastian Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; ArgentinaFil: Mancini, Micaela Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Exactas. Instituto Multidisciplinario de Ecosistemas y Desarrollo Sustentable; ArgentinaFil: Russo, Maria Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; ArgentinaFil: Vianna, María Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; ArgentinaFil: Scorsetti, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentin

La química orgánica en armonía con nuestras sensaciones

In this work the development and the results of a practical work are shown. The practical work has been designed for the teaching of organic chemistry in a high school teaching course. The selected theme is related to the perfume industry. Its main goal was related to promoting the study of the chemical discipline. This work was carried out within five weeks. It contemplates the fundamental abilities of reading, writing, orality and critical thinking. The results obtained indicate that it was possible to consolidate contents, relate them to others previously developed, improve the writing and presentation of reports, develop collaborative work, and improve skills in the handling of laboratory material.Fil: Guntero, Vanina Alejandra. Universidad Nacional del Litoral. Instituto de Química Aplicada del Litoral. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Química Aplicada del Litoral.; ArgentinaFil: Peralta, Micaela Belén. Universidad Tecnológica Nacional. Facultad Regional San Francisco. Departamento de Ingeniería Electromecánica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferretti, Cristián Alejandro. Universidad Nacional del Litoral. Instituto de Química Aplicada del Litoral. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Química Aplicada del Litoral.; ArgentinaFil: Mancini, Pedro Maximo Emilio. Universidad Nacional del Litoral. Instituto de Química Aplicada del Litoral. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Química Aplicada del Litoral.; ArgentinaFil: Kneeteman, Maria Nelida. Universidad Nacional del Litoral. Instituto de Química Aplicada del Litoral. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Química Aplicada del Litoral.; Argentin

Development of a modified transformation platform for apomixis candidate genes research in Paspalum notatum (bahiagrass)

Abstract The aim of this work was to improve existing transformation protocols and to transform specific genotypes of Paspalum notatum (bahiagrass) for functional analyses of candidate genes involved in reproduction. Three different explants were assayed for in vitro plant regeneration: mature seeds, mature embryos, and shoot meristems. Plant regeneration was achieved with all explant types, but mature seeds produced the optimal rate (78.0%) and were easiest to manipulate. A method based on serial re-induction of calli from meristems of the regenerated lines was also developed, which could be useful in plant breeding strategies pursuing somaclonal variation. Transient transformation experiments were performed on calli obtained from mature seeds using a compressed helium gene gun. Transient transformation constructs included anthocyanin-synthesis genes cloned under the CAMV 35S promoter and an enhanced green fluorescent protein gene (egfp) driven by the rice actin1 (act1) promoter. Selection curves for ammonium glufosinate were developed in order to determine the optimal selective pressure for stable transformation (1.0 mg/L). Stable co-transformation experiments were carried out with two different constructs containing: (1) the reporter egfp gene cloned under the rice act1 promoter and (2) the selector bar gene driven by the ubiquitin promoter. A total of 27 (64.2%) transgenic plants out of 42 resistant plants analyzed were obtained. The presence of the transgenes in regenerated plants was confirmed by polymerase chain reaction and DNA gel blot analysis. Gene expression was demonstrated by eGFP fluorescence detection and in vivo assays for ammonium glufosinate tolerance. This platform is being used to generate transgenic plants of P. notatum to analyze the function of apomixis-associated candidate genes.Fil: Mancini, Micaela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; ArgentinaFil: Woitovich Valetti, Nadia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; ArgentinaFil: Permingeat, Hugo Raúl. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; ArgentinaFil: Podio, Maricel. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Siena, Lorena Adelina. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; ArgentinaFil: Ortiz, Juan Pablo Amelio. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); ArgentinaFil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; ArgentinaFil: Felitti, Silvina Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Cientifico Tecnológico Rosario; Argentin

Characterization and expression analysis of Somatic Embryogenesis Receptor Kinase (SERK) genes in sexual and apomictic Paspalum notatum

The Somatic Embryogenesis Receptor Kinase (SERK) gene plays a fundamental role in somatic embryogenesis of angiosperms, and is associated with apomixis in Poa pratensis. The objective of this work was to isolate, characterize and analyze the expression patterns of SERK genes in apomictic and sexual genotypes of Paspalum notatum. A conserved 200-bp gene fragment was amplified from genomic DNA with heterologous primers, and used to initiate a chromosomal walking strategy for cloning the complete sequence. This procedure allowed the isolation of two members of the P. notatum SERK family; PnSERK1, which is similar to PpSERK1, and PnSERK2, which is similar to ZmSERK2 and AtSERK1. Phylogenetic analyses indicated that PnSERK1 and PnSERK2 represent paralogous sequences. Southern-blot hybridization indicated the presence of at least three copies of SERK genes in the species. qRT-PCR analyses revealed that PnSERK2 was expressed at significantly higher levels than PnSERK1 in roots, leaves, reproductive tissues and embryogenic calli.Moreover, in situ hybridization experiments revealed that PnSERK2 displayed a spatially and chronologically altered expression pattern in reproductive organs of the apomictic genotype with respect to the sexual one. PnSERK2 is expressed in nucellar cells of the apomictic genotype at meiosis, but only in the megaspore mother cell in the sexual genotype. Therefore, apomixis onset in P. notatum seems to be correlated with the expression of PnSERK2 in nucellar tissue.Fil: Podio, Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina; Universidad Nacional de Rosario. Facultad de Cs.agrarias. Laboratorio de Biologia Molecular; Argentina;Fil: Felitti, Silvina Andrea. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Laboratorio de Biologia Molecular; Argentina;Fil: Siena, Lorena Adeli. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Laboratorio de Biologia Molecular; Argentina;Fil: Delgado Benarroch, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina; Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina;Fil: Mancini, Micaela. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Laboratorio de Biologia Molecular; Argentina;Fil: Seijo, Jose Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina;Fil: Gonzalez, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina;Fil: Pessino, Silvina Claudia. Universidad Nacional de Rosario. Facultad de Cs.agrarias. Laboratorio de Biologia Molecular; Argentina;Fil: Ortiz, Juan Pablo Amelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Nordeste. Instituto de Botánica del Nordeste (i); Argentina; Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias; Argentina; Universidad Nacional de Rosario. Facultad de Cs.agrarias. Laboratorio de Biologia Molecular; Argentina

Prolonged higher dose methylprednisolone vs. conventional dexamethasone in COVID-19 pneumonia: a randomised controlled trial (MEDEAS)

Dysregulated systemic inflammation is the primary driver of mortality in severe COVID-19 pneumonia. Current guidelines favor a 7-10-day course of any glucocorticoid equivalent to dexamethasone 6 mg·day-1. A comparative RCT with a higher dose and a longer duration of intervention was lacking

Bowel preparation for elective colorectal resection: multi-treatment machine learning analysis on 6241 cases from a prospective Italian cohort

background current evidence concerning bowel preparation before elective colorectal surgery is still controversial. this study aimed to compare the incidence of anastomotic leakage (AL), surgical site infections (SSIs), and overall morbidity (any adverse event, OM) after elective colorectal surgery using four different types of bowel preparation. methods a prospective database gathered among 78 Italian surgical centers in two prospective studies, including 6241 patients who underwent elective colorectal resection with anastomosis for malignant or benign disease, was re-analyzed through a multi-treatment machine-learning model considering no bowel preparation (NBP; No. = 3742; 60.0%) as the reference treatment arm, compared to oral antibiotics alone (oA; No. = 406; 6.5%), mechanical bowel preparation alone (MBP; No. = 1486; 23.8%), or in combination with oAB (MoABP; No. = 607; 9.7%). twenty covariates related to biometric data, surgical procedures, perioperative management, and hospital/center data potentially affecting outcomes were included and balanced into the model. the primary endpoints were AL, SSIs, and OM. all the results were reported as odds ratio (OR) with 95% confidence intervals (95% CI). results compared to NBP, MBP showed significantly higher AL risk (OR 1.82; 95% CI 1.23-2.71; p = .003) and OM risk (OR 1.38; 95% CI 1.10-1.72; p = .005), no significant differences for all the endpoints were recorded in the oA group, whereas MoABP showed a significantly reduced SSI risk (OR 0.45; 95% CI 0.25-0.79; p = .008). conclusions MoABP significantly reduced the SSI risk after elective colorectal surgery, therefore representing a valid alternative to NBP

Abdominal drainage after elective colorectal surgery: propensity score-matched retrospective analysis of an Italian cohort

background: In italy, surgeons continue to drain the abdominal cavity in more than 50 per cent of patients after colorectal resection. the aim of this study was to evaluate the impact of abdominal drain placement on early adverse events in patients undergoing elective colorectal surgery. methods: a database was retrospectively analysed through a 1:1 propensity score-matching model including 21 covariates. the primary endpoint was the postoperative duration of stay, and the secondary endpoints were surgical site infections, infectious morbidity rate defined as surgical site infections plus pulmonary infections plus urinary infections, anastomotic leakage, overall morbidity rate, major morbidity rate, reoperation and mortality rates. the results of multiple logistic regression analyses were presented as odds ratios (OR) and 95 per cent c.i. results: a total of 6157 patients were analysed to produce two well-balanced groups of 1802 patients: group (A), no abdominal drain(s) and group (B), abdominal drain(s). group a versus group B showed a significantly lower risk of postoperative duration of stay &gt;6 days (OR 0.60; 95 per cent c.i. 0.51-0.70; P &lt; 0.001). a mean postoperative duration of stay difference of 0.86 days was detected between groups. no difference was recorded between the two groups for all the other endpoints. conclusion: this study confirms that placement of abdominal drain(s) after elective colorectal surgery is associated with a non-clinically significant longer (0.86 days) postoperative duration of stay but has no impact on any other secondary outcomes, confirming that abdominal drains should not be used routinely in colorectal surgery