150 research outputs found

    Autoezagutza emozionala eta mindfulness-a eskolan

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    [EUS] Lan honetan Lehen Hezkuntzako laugarren mailan egindako proposamen didaktikoa aurkeztuko da. Helburua ikasgelan autoezagutza emozionala lantzea izango da, ikasleen garapen integralerako oso garrantzitsua baita. Hori abiapuntutzat hartuta, mindfulness teknika erabili da gaitasun emozionala garatzeko baliabide gisa. Esku-hartzea diseinatzeko, informazio bilaketa egin da; halaber, ebaluazio bat egin da ikasleen aurrerapenak aztertzeko. Emaitzei dagokienez, aipatzekoa da, ikasleak gai izan direla sentimendu eta emozioak identifikatzeko, izendatzeko eta horiek gorputzarekin duten loturaz ohartzeko, baita autoerregulaziorako teknikak erabilita sentimendu desatseginak askatzeko ere.[ES] Este trabajo presenta una propuesta didáctica realizada en cuarto de primaria. El propósito es trabajar el autoconocimiento emocional en el aula, puesto que es muy importante para el desarrollo integral del alumnado. Así pues, se ha utilizado el mindfulness como recurso para desarrollar la competencia emocional. Con el objetivo de diseñar la intervención, se ha realizado una búsqueda de información; además, se ha elaborado una evaluación para analizar los progresos del alumnado. Respecto a los resultados, los participantes han logrado identificar y nombrar emociones, observar la relación entre cuerpo y emoción y liberarse de sentimientos desagradables utilizando técnicas de autorregulación.[EN] This academic work introduces a didactic proposal carried out in the fourth year of Primary School. The aim is to work on emotional self-knowledge as it is crucial for the integral development of students. That way, the mindfulness has been used as a way of developing the emotional competence. The process of elaboration required to undertake a research; moreover, an evaluation has been designed to analyse the progress of students. As for the results, the participants were able to identify and name emotions, observe the relation between emotions and body, and to release from unpleasant feelings using self-regulation techniques

    Hediste diversicolor itsas poliketoaren azterketa biokimiko eta histologikoa

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    [EUS] Hediste diversicolor poliketo espeziearekin burututako eta dietarekin lotutako azterketa esperimental bat azaltzen da. Baita poliketoaren populazio basati batean eta haztegiko populazioan egindako azterketa biokimiko eta histologikoak eta hauen arteko desberdintasunak.[EN] Hediste diversicolor is a key species in estuaries amongst polychaetes, because it is an important food resource for a variety of animals. Hatchery populations were compared with wild samples histologically and biochemically, analyzing the distribution of reserves of materials

    Autoezagutza emozionala eta mindfulness-a eskolan

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    [EUS] Lan honetan Lehen Hezkuntzako laugarren mailan egindako proposamen didaktikoa aurkeztuko da. Helburua ikasgelan autoezagutza emozionala lantzea izango da, ikasleen garapen integralerako oso garrantzitsua baita. Hori abiapuntutzat hartuta, mindfulness teknika erabili da gaitasun emozionala garatzeko baliabide gisa. Esku-hartzea diseinatzeko, informazio bilaketa egin da; halaber, ebaluazio bat egin da ikasleen aurrerapenak aztertzeko. Emaitzei dagokienez, aipatzekoa da, ikasleak gai izan direla sentimendu eta emozioak identifikatzeko, izendatzeko eta horiek gorputzarekin duten loturaz ohartzeko, baita autoerregulaziorako teknikak erabilita sentimendu desatseginak askatzeko ere.[ES] Este trabajo presenta una propuesta didáctica realizada en cuarto de primaria. El propósito es trabajar el autoconocimiento emocional en el aula, puesto que es muy importante para el desarrollo integral del alumnado. Así pues, se ha utilizado el mindfulness como recurso para desarrollar la competencia emocional. Con el objetivo de diseñar la intervención, se ha realizado una búsqueda de información; además, se ha elaborado una evaluación para analizar los progresos del alumnado. Respecto a los resultados, los participantes han logrado identificar y nombrar emociones, observar la relación entre cuerpo y emoción y liberarse de sentimientos desagradables utilizando técnicas de autorregulación.[EN] This academic work introduces a didactic proposal carried out in the fourth year of Primary School. The aim is to work on emotional self-knowledge as it is crucial for the integral development of students. That way, the mindfulness has been used as a way of developing the emotional competence. The process of elaboration required to undertake a research; moreover, an evaluation has been designed to analyse the progress of students. As for the results, the participants were able to identify and name emotions, observe the relation between emotions and body, and to release from unpleasant feelings using self-regulation techniques

    De Tropeles y Tropelías : perspectivas de uma tradução comentada

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    Trabalho de conclusão de curso (graduação)—Universidade de Brasília, Instituto de Letras, Departamento de Línguas Estrangeiras e Tradução, 2016.O presente Projeto Final de Curso consiste em uma discussão teórica e prática a partir da tradução comentada de fábulas e contos presentes no livro De Tropeles y Tropelías (1971), obra do escritor nicaraguense Sergio Ramírez. Por tratar-se de uma tradução literária, haverá também uma apresentação de aspectos relevantes e necessários que foram utilizados durante o processo. O objetivo deste trabalho consiste em uma aproximação intercultural, motivada por uma intenção ideológica.El presente Proyecto Final de Curso consiste en una discusión teórica y práctica a partir de la traducción comentada de fábulas y cuentos presentes en el libro De Tropeles y Tropelías (1971), obra del escritor nicaragüense Sergio Ramírez. Por tratarse de una traducción literaria, habrá, además, una presentación de aspectos relevantes y necesarios que se utilizaron durante el proceso. El objetivo de este trabajo consiste en una aproximación intercultural, motivada por una intención ideológica

    Identification of novel genes involved in petunia flower development using transcript profiling and reverse genetics

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    [ENG] Petals are a key element on plant life cycle as, in many species, they attract pollinators, thus aiding to reproduction. Furthermore, they have economic importance in ornamental crops. In the present study, petal transcriptional patterns were compared within the ower organs in Arabidopsis thaliana. It was found that catalytic molecular functions were overrepresented in petals. A shortlist comprising the top ten di_erentially expressed genes in petals were mapped to the model species with industrial value Petunia hybrida, and further downregulated by RNAi. The silencing phenotypes found permitted to assign functions in petal development to seven novel genes: when silenced, they triggered alterations on ower size and shape (PhCYP76, PhNPH3, PhFeSOD, PhXTH, PhCYP96 and PhWAK), petal smoothness (PhPRA), color (PhNPH3 and PhWAK) and symmetry (PhCYP76). Pleiotropic phenotypes were found, such as changes in root morphology and leaf color (PhCYP76), ower number, capsule and seed morphology (PhCYP96) and plant height (PhCYP76 and PhCYP96). To accomplish the experimental design, three methods were developed. First, the \pESTle" management system that assembles, annotates, stores and serves expressed sequence tag data. Second, a reference gene selection for real time PCR experiments that includes a new method for stability estimation based on rank aggregation of published algorithms, and concludes that a normalization factor with two members of EF1_, SAND, CYP or RAN1 is stable enough under most conditions. And third, a PCR e_ciency estimator based on amplicon characteristics which allows e_ciency-driven primer design in a Web tool. [SPA] La corola es un elemento básico en el ciclo de vida vegetal puesto que, en muchos casos, atrae a polinizadores que intervienen en su propagación. Además, posee un valor económico en especies ornamentales. En el presente trabajo se realizó un análisis comparativo contrastando el transcriptoma de pétalos frente al resto de órganos orales de Arabidopsis thaliana. Como resultado se observó la preponderancia de genes involucrados en funciones catalíticas. Aún de dilucidar el rol de estos genes, se escogieron aquellos nueve con mayor expresión diferencial y se silenciaron de forma estable mediante ARN de interferencia. De esta forma, el análisis fenotípico ha permitido asignar un papel a siete nuevos genes: al ser silenciados, provocan alteraciones en la forma y tamaño (PhCYP76, PhNPH3, PhFeSOD, PhXTH, PhCYP96 y PhWAK), textura (PhPRA), color (PhNPH3 y Ph- WAK) y simetría (PhCYP76) de los pétalos; así como el color de las hojas y la morfología radicular (PhCYP76), el número de ores y la altura de la planta (PhCYP76 y PhCYP96). Aún de respaldar el diseño experimental se desarrollaron tres métodos. En primer lugar, el gestor _pESTle_, que aúna el ensamblaje, anotación, almacenamiento y difusión de ESTs. En segundo lugar, la selección de genes de referencia para PCR en tiempo real, que reúne un nuevo método para su evaluación basado en la agregación de rangos; y la descripción de la pertinencia de emplear un factor de normalización con dos genes de entre EF1_, SAND, CYP y RAN1 en la mayor parte de los casos. Finalmente, se produjo el estimador _pcrE_ciency_, que proporciona un entorno de diseño de cebadores que simultáneamente predice su eficiencia.Universidad Politécnica de Cartagen

    Massachusetts Adult Basic Education and ESOL Distance Learning Programs: How to Assess Student Readiness for Distance Learning?

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    This report presents the findings from the pilot screening process prepared by the Center for Social Policy (CSP) and implemented during December 2008-March 2009 by the adult basic education (ABE) online learning programs in MA which are funded by the Department of Elementary and Secondary Education (ESE). The purposes of the pilot process were: To determine the effectiveness of screening tools in assessing participant characteristics, such as the skills, demographics, and life circumstances that are associated with success in online learning; To identify supports and interventions which students need in order to persist and succeed in online learning. At the end of the first round of research during April-August 2008, CSP research team determined that readiness and success of adult students in online learning are embedded indeed in multiple factors both at the individual and the contextual level and these multiple factors are inter-connected with one another. The individual factors include various demographic variables, social characteristics, life situations, learning styles, motivation, computer skills, and cognitive and meta-cognitive skills. The contextual factors include the role of the instructor, the variability and access to the materials and technology being used, the type of program, i.e. GED, Pre-GED and ESOL, the specific requirements of the course being taught and the curriculum design (Petty, Johnston, & Shafer, 2004). CSP researchers developed the Interactive-Constructivist Model of ABE Distance Learning (DL) based on the findings of the first round of research. The Interactive-Constructivist approach conceives learning as a social process. Learning does not happen in a vacuum, but rather in a social context (Kahraman, Mallona, Friedman, Platt, & Kahan, 2008)

    Wanderer, an interactive viewer to explore DNA methylation and gene expression data in human cancer

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    BACKGROUND: The Cancer Genome Atlas (TCGA) offers a multilayered view of genomics and epigenomics data of many human cancer types. However, the retrieval of expression and methylation data from TCGA is a cumbersome and time-consuming task.RESULTS:Wanderer is an intuitive Web tool allowing real time access and visualization of gene expression and DNA methylation profiles from TCGA. Given a gene query and selection of a TCGA dataset (e.g., colon adenocarcinomas), the Web resource provides the expression profile, at the single exon level, and the DNA methylation levels of HumanMethylation450 BeadChip loci inside or in the vicinity of the queried gene. Graphic and table outputs include individual and summary data as well as statistical tests, allowing the comparison of tumor and normal profiles and the exploration along the genomic locus and across tumor collections. CONCLUSIONS: Wanderer offers a simple interface to straightforward access to TCGA data, amenable to experimentalists and clinicians without bioinformatics expertise. Wanderer may be accessed at http://maplab.catwanderer

    Validation of reference genes for quantitative real-time PCR during leaf and flower development in Petunia hybrida

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    <p>Abstract</p> <p>Background</p> <p>Identification of genes with invariant levels of gene expression is a prerequisite for validating transcriptomic changes accompanying development. Ideally expression of these genes should be independent of the morphogenetic process or environmental condition tested as well as the methods used for RNA purification and analysis.</p> <p>Results</p> <p>In an effort to identify endogenous genes meeting these criteria nine reference genes (RG) were tested in two Petunia lines (Mitchell and V30). Growth conditions differed in Mitchell and V30, and different methods were used for RNA isolation and analysis. Four different software tools were employed to analyze the data. We merged the four outputs by means of a non-weighted unsupervised rank aggregation method. The genes identified as optimal for transcriptomic analysis of Mitchell and V30 were <it>EF1α </it>in Mitchell and <it>CYP </it>in V30, whereas the least suitable gene was <it>GAPDH </it>in both lines.</p> <p>Conclusions</p> <p>The least adequate gene turned out to be <it>GAPDH </it>indicating that it should be rejected as reference gene in Petunia. The absence of correspondence of the best-suited genes suggests that assessing reference gene stability is needed when performing normalization of data from transcriptomic analysis of flower and leaf development.</p
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