Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants

Little is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identify-ing a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target strategies). These methodologies should be robust and simple. This is particularly challenging for solid samples, as reliable extraction and clean-up techniques should be combined to obtain an optimal result. Hence, the present study focuses on the development of an analytical methodology that allows the screening of a wide range of organic chemicals in brain and brain tumor samples. This protocol was based on a solid-liquid extraction based on bead beating, solid-phase extraction clean-up with multi-layer mixed-mode cartridges, reconstitution and LC -HRMS analysis. To evaluate the performance of the extraction methodology, a set of 66 chemicals (e.g., pharmaceuticals, biocides, or plasticizers, among others) with a wide range of physicochemical properties was employed. Quality control parameters (i.e., linear range, sensitivity, matrix effect (ME%), and recoveries (R%)) were calculated and satisfactory results were obtained for them (e.g., R% within 60-120% for 32 chemicals, or ME% higher than 50% (signal suppression) for 79% of the chemicals)

Multiproject–multicenter evaluation of automatic brain tumor classification by magnetic resonance spectroscopy

Contains fulltext : 75361.pdf (publisher's version ) (Open Access)14 p

Orbital lymphoma: imaging features and differential diagnosis

Abstract Purpose Patterns of orbital lymphoma at diagnosis and follow-up are described. We also discuss differential diagnosis of orbital masses. Materials and methods This pictorial review contains 19 cases of orbital lymphoma before and after treatment. Superior-lateral quadrant and extra-conal location were observed predominantly. Effective response after treatment was presented on follow-up imaging, although few local relapses were found. Further follow-up showed no changes of residual images. Discussion Location of orbital masses can help in the differential diagnosis. Moreover, imaging features of lymphoma at diagnosis can be useful in planning surgical biopsy. Pattern of follow-up described may be relevant on monitoring imaging. Teaching points • Orbital lymphoma involves mainly superior-lateral quadrant and the orbital structures inside. • Location of retrobulbar mass-like lesions are useful information in the differential diagnosis. • Satisfactory response is detected after treatment, however relapse is noted, so follow-up is needed

MR dynamic-susceptibility-contrast perfusion metrics in the presurgical discrimination of adult solitary intra-axial cerebellar tumors

Adult solitary intra-axial cerebellar tumors are uncommon. Their presurgical differentiation based on neuroimaging is crucial, since management differs substantially. Comprehensive full assessment of MR dynamic-susceptibility-contrast perfusion-weighted imaging (DSC-PWI) may reveal key differences between entities. This study aims to provide new insights on perfusion patterns of these tumors and to explore the potential of DSC-PWI in their presurgical discrimination.Albert Pons-Escoda and Carles Majos acknowledge a grant from the Instituto de Salud Carlos III (PI20/00360). Pablo Gago-Ferrero acknowledges a Ramón y Cajal grant (RYC2019-027913-I). The authors want to acknowledge Pilar Lopez-Ubeda, PhD, from HT Medica. She participated in retrospective patient recruitment through advanced natural language processing methods. The authors thank CERCA Programme/Generalitat de Catalunya for institutional support.Peer reviewe