Promotion of embryonic cortico-cerebral neuronogenesis by miR-124

Background: Glutamatergic neurons of the murine cerebral cortex are generated within periventricular proliferative layers of the embryonic pallium, directly from apical precursors or indirectly via their basal progenies. Cortical neuronogenesis is the result of different morphogenetic subroutines, including precursor proliferation and death, changes in histogenetic potencies, and post-mitotic neuronal differentiation. Control of these processes is extremely complex, involving numerous polypeptide-encoding genes. Moreover, many so-called 'non-coding genes' are also expressed in the developing cortex. Currently, their implication in corticogenesis is the subject of intensive functional studies. A subset of them encodes microRNAs (miRNAs), a class of small RNAs with complex biogenesis that regulate gene expression at multiple levels and modulate histogenetic progression and are implicated in refinement of positional information. Among the cortical miRNAs, miR-124 has been consistently shown to promote neuronogenesis progression in a variety of experimental contexts. Some aspects of its activity, however, are still controversial, and some have to be clarified. An in depth in vivo characterization of its function in the embryonic mammalian cortex is still missing. Results: By integrating locked nucleic acid (LNA)-oligo in situ hybridization, electroporation of stage-specific reporters and immunofluorescence, we reconstructed the cortico-cerebral miR-124 expression pattern during direct neuronogenesis from apical precursors and indirect neuronogenesis via basal progenitors. The miR-124 expression profile in the developing embryonic cortex includes an abrupt upregulation in apical precursors undergoing direct neuronogenesis as well as a two-step upregulation in basal progenitors during indirect neuronogenesis. Differential post-transcriptional processing seems to contribute to this pattern. Moreover, we investigated the role of miR-124 in embryonic corticogenesis by gain-of-function approaches, both in vitro, by lentivirus-based gene transfer, and in vivo, by in utero electroporation. Following overexpression of miR-124, both direct neuronogenesis and progression of neural precursors from the apical to the basal compartment were stimulated. Conclusion: We show that miR-124 expression is progressively up-regulated in the mouse embryonic neocortex during the apical to basal transition of neural precursor cells and upon their exit from cell cycle, and that miR-124 is involved in the fine regulation of these processes

Weak first order transition in the three-dimensional site-diluted Ising antiferromagnet in a magnetic field

We perform intensive numerical simulations of the three-dimensional site-diluted Ising antiferromagnet in a magnetic field at high values of the external applied field. Even if data for small lattice sizes are compatible with second-order criticality, the critical behavior of the system shows a crossover from second-order to first-order behavior for large system sizes, where signals of latent heat appear. We propose "apparent" critical exponents for the dependence of some observables with the lattice size for a generic (disordered) first-order phase transition.Comment: Final version, accepted for publicatio

Rock magnetism and palaeomagnetism of the Montalbano Jonico section (Italy): evidence for late diagenetic growth of greigite and implications for magnetostratigraphy

The Montalbano Jonico (MJ) section, cropping out in Southern Italy, represents a potential candidate to define the Lower/Middle Pleistocene boundary and it has been proposed as a suitable Global Stratotype Section and Point (GSSP) of the Ionian Stage (Middle Pleistocene). The MJ section is the only continuous benthic and planktonic δ18O on-land reference in the Mediterranean area for the Mid-Pleistocene transition, spanning an interval between about 1240 and 645 ka. Combined biostratigraphy and sapropel chronology, tephra stratigraphy and complete high-resolution benthic and planktonic foraminiferal stable oxygen isotope records already provide a firm chronostratigraphic framework for the MJ section. However, magnetostratigraphy was still required to precisely locate the Brunhes-Matuyama transition and to mark the GSSP for the Ionian stage. We carried out a palaeomagnetic study of a subsection (Ideale section) of the MJ composite section, sampling 61 oriented cores from 56 stratigraphic levels spread over a ca. 80-m-thick stratigraphic interval that correlates to the oxygen isotopic stage 19 and should therefore include the Brunhes-Matuyama reversal. The palaeomagnetic data indicate a stable and almost single-component natural remanent magnetization (NRM). A characteristic remanent magnetization (ChRM) was clearly identified by stepwise demagnetization of the NRM. The ChRM declination values vary around 0◦ and the ChRM inclination around the expected value (59◦) for a geocentric axial dipole field at the sampling locality. This result indicates that the section has been remagnetized during the Brunhes Chron. A preliminary study of 27 additional not azimuthally oriented hand samples, collected at various levels from other parts of the MJ composite section, indicates that all the samples are of normal polarity and demonstrates that the remagnetization is widespread across the whole exposed stratigraphic sequence. A series of specific rock magnetic techniques were then applied to investigate the nature of the main magnetic carrier in the study sediments, and they suggest that the main magnetic mineral in the MJ section is the iron sulphide greigite (Fe3S4). Scanning electron microscope observations and elemental microanalysis reveal that greigite occurs both as individual euhedral crystals and in iron sulphides aggregates filling voids in the clay matrix. Therefore, we infer that the remagnetization of the section is due to the late-diagenetic growth of greigite under reducing conditions, most likely resulting in the almost complete dissolution of the original magnetic minerals. Iron sulphide formation in the MJ section can be linked to migration of mineralized fluids. Our inferred timing of the remagnetization associated with greigite growth represents the longest remanence acquisition delay documented in greigite-bearing clays of the Italian peninsula so far

Improving spatial predictions of taxonomic, functional and phylogenetic diversity

In this study, we compare two community modelling approaches to determine their ability to predict the taxonomic, functional and phylogenetic properties of plant assemblages along a broad elevation gradient and at a fine resolution. The first method is the standard stacking individual species distribution modelling (SSDM) approach, which applies a simple environmental filter to predict species assemblages. The second method couples the SSDM and macroecological modelling (MEMSSDM-MEM) approaches to impose a limit on the number of species co-occurring at each site. Because the detection of diversity patterns can be influenced by different levels of phylogenetic or functional trees, we also examine whether performing our analyses from broad to more exact structures in the trees influences the performance of the two modelling approaches when calculating diversity indices. We found that coupling the SSDM with the MEM improves the overall predictions for the three diversity facets compared with those of the SSDM alone. The accuracy of the SSDM predictions for the diversity indices varied greatly along the elevation gradient, and when considering broad to more exact structure in the functional and phylogenetic trees, the SSDM-MEM predictions were more stable. SSDM-MEM moderately but significantly improved the prediction of taxonomic diversity, which was mainly driven by the corrected number of predicted species. The performance of both modelling frameworks increased when predicting the functional and phylogenetic diversity indices. In particular, fair predictions of the taxonomic composition by SSDM-MEM led to increasingly accurate predictions of the functional and phylogenetic indices, suggesting that the compositional errors were associated with species that were functionally or phylogenetically close to the correct ones; however, this did not always hold for the SSDM predictions.Synthesis. In this study, we tested the use of a recently published approach that couples species distribution and macroecological models to provide the first predictions of the distribution of multiple facets of plant diversity: taxonomic, functional and phylogenetic. Moderate but significant improvements were obtained; thus, our results open promising avenues for improving our ability to predict the different facets of biodiversity in space and time across broad environmental gradients when functional and phylogenetic information is available

Unveiling the nature of INTEGRAL objects through optical spectroscopy. II. The nature of four unidentified sources

We present the results of our optical spectrophotometric campaign ongoing at the Astronomical Observatory of Bologna in Loiano (Italy) on hard X-ray sources detected by INTEGRAL. We observed spectroscopically the putative optical counterparts of four more INTEGRAL sources, IGR J12391-1610, IGR J18406-0539, 2E 1853.7+1534 and IGR J19473+4452. These data have allowed us to determine their nature, finding that IGR J12391-1610 (=LEDA 170194) and IGR J19473+4452 are Seyfert 2 galaxies at redshifts z = 0.036 and z = 0.053, respectively, IGR J18406-0539 (=SS 406) is a Be massive X-ray binary located at about 1.1 kpc from Earth, and 2E 1853.7+1534 is a Type 1 Seyfert galaxy with z = 0.084. Physical parameters for these objects are also evaluated by collecting and discussing the available multiwavelength information. The determination of the extragalactic nature of a substantial fraction of sources inside the INTEGRAL surveys underlines the importance of hard X-ray observations for the study of background Active Galactic Nuclei located beyond the `Zone of Avoidance' of the Galactic Plane.Comment: 10 pages, 2 figures, 2 tables, accepted for publication on A&A, main journal. The quality of Fig. 1 was degraded to fit the arXiv uploads size limit

Climate variability during MIS 20–18 as recorded by alkenone-SST and calcareous plankton in the Ionian Basin (central Mediterranean)

This study shows the first Mediterranean high-resolution record of alkenone-derived sea surface temperature (SST) in the marine sediments outcropping at the Ideale section (IS) (southern Italy, central Mediterranean) from late marine isotope stage (MIS) 20 - through early MIS 18. The SST pattern evidences glacial-interglacial up to submillennial-scale temperature variation, with lower values (~13 °C) in late MIS 20 and substage 19b, and higher values (up to 21 °C) in MIS 19c and in the interstadials of MIS 19a. The SST data are combined with the new calcareous plankton analysis and the available, chronologically well-constrained carbon and oxygen isotope records in the IS. The multi-proxy approach, together with the location of the IS near the Italian coasts, the lower circalittoral-upper bathyal depositional setting, and high sedimentation rate allow to document long-and short-term paleoenvironmental modifications (sea level, rainfall, inorganic/organic/fresh water input to the basin), as a response to regional and global climate changes. The combined proxies reveal the occurrence of a terminal stadial event in late MIS 20 (here Med-HTIX), and warm-cold episodes (here Med-BATIX and Med-YDTIX) during Termination IX (TIX), which recall those that occurred through the last termination (TI). During these periods and the following ghost sapropel layer (insolation cycle 74, 784 ka) in the early MIS 19, high frequency internal changes are synchronously recorded by all proxies. The substage MIS 19c is warm but quite unstable, with several episodes of paleoenvironmental changes, associated with fluctuating tropical-subtropical water inflow through the Gibraltar Strait, variations of the cyclonic regime in the Ionian basin, and the southward shift of westerly winds and winter precipitation over southern Europe and Mediterranean basin. Three high-amplitude millennial-scale oscillations in the patterns of SST and calcareous plankton key taxa during MIS 19a are interpreted as linked to changes in temperature as well as in salinity due to periodical water column stratification and mixing. The main processes involved in the climate variability include changes in oceanographic exchanges through the Gibraltar Strait during modulations of Atlantic meridional overturning circulation and/or variations in atmospheric dynamics related to the influence of westerly and polar winds acting in the paleo-Ionian basin. A strong climate teleconnection between the North Atlantic and Mediterranean is discussed, and a prominent role of atmospheric processes in the central Mediterranean is evidenced by comparing data sets at the IS with Italian and extra-Mediterranean marine and terrestrial records

Sars-cov-2 and skin: The pathologist’s point of view

The SARS-CoV-2 pandemic has dramatically changed our lives and habits. In just a few months, the most advanced and efficient health systems in the world have been overwhelmed by an infectious disease that has caused 3.26 million deaths and more than 156 million cases worldwide. Although the lung is the most frequently affected organ, the skin has also resulted in being a target body district, so much so as to suggest it may be a real “sentinel” of COVID-19 disease. Here we present 17 cases of skin manifestations studied and analyzed in recent months in our Department; immunohistochemical investigations were carried out on samples for the S1 spike-protein of SARS-CoV-2, as well as electron microscopy investigations showing evidence of virions within the constituent cells of the eccrine sweat glands and the endothelium of small blood vessels. Finally, we conduct a brief review of the COVID-related skin manifestations, confirmed by immunohistochemistry and/or electron microscopy, described in the literature

Swift XRT Observations of the Breaking X-ray Afterglow of GRB 050318

We report the results of Swift X-Ray Telescope (XRT) observations of GRB 050318. This event triggered the Burst Alert Telescope (BAT) aboard Swift and was followed-up with XRT and UVOT for 11 consecutive orbits starting from 54 minutes after the trigger. A previously unknown fading X-ray source was detected and accurately monitored. The source was found to decrease in intensity with time and a clear temporal break occurring at ~18000 s after the trigger was observed. The X-ray light curve was found to be consistent with a broken power-law with decay indices -1.17 +/- 0.08 and -2.10 (+0.22) (-0.24) before and after the break. The spectrum of the X-ray afterglow was well described by a photoelectrically absorbed power-law with energy index of -1.09 +/-0.09. No evidence of spectral evolution was found. We compare these results with those obtained with UVOT and separately reported and refine the data analysis of BAT. We discuss our results in the framework of a collimated fireball model and a synchrotron radiation emission mechanism. Assuming the GRB redshift derived from the farthest optical absorption complex (z = 1.44), the event is fully consistent with the E_p-E_iso correlation.Comment: Accepted for publication in A&A Letters (5 pages, 3 figures