Effects of daily herbage allowance and stage of lactation on the intake and performance of dairy cows in early summer

peer-reviewedThis research was part-funded by European Union Structural Funds (EAGGF).The aim of this study was to investigate the relationship between daily herbage allowance (DHA) and the performance of dairy cows at two stages of lactation. Spring-calving (n=42, mean calving date 17 February) and autumn-calving (n=42, mean calving date 22 September) Friesian cows were divided into three equal groups and assigned to three levels of DHA (above a cutting height of 35 mm), 17 (L), 20 (M) and 23 (H) kg of dry matter (DM) per head, from late April to late June, 1996. The spring-calving cows grazed to sward heights (mm) of 47, 56 and 65 (s.e. 0.6) and residual herbage organic matter (OM) masses (above 35 mm) of 294, 408 and 528 (s.e. 12.1) kg/ha for L, M and H, respectively. The autumn-calving cows grazed to corresponding sward heights of 51, 60 and 69 (s.e. 1.1) mm and leftresidual herbage OM masses of 364, 445 and 555 (s.e. 12.9) kg/ha for L, M and H, respectively. Pastures were mechanically topped post grazing. Spring-calving cows consumed 13.3, 14.7 and 15.5 kg OM (s.e. 0.47) per day, and autumn-calving cows consumed 13.3, 13.8 and 14.9 kg OM (s.e. 0.43) per day for L, M and H, respectively. Mean daily solids-corrected milk yield was 23.1, 23.8 and 24.8 (s.e. 0.34) kg for the spring-calving cows, and 17.5, 18.4 and 18.7 (s.e. 0.35) kg for the autumncalving cows, for L, M and H, respectively. Milk yield could be predicted from preexperimental yield (PMY) and daily herbage organic matter allowance (DOMA, kg) according to the following equation: y = −1.13 + 0.76 (s.e. 0.030) PMY + 0.22 (s.e. 0.057) DOMA (r.s.d. 1.32, R2 0.89). The results indicate that high individual cow and herd production levels can be achieved from high quality herbage alone during early summer at a DHA of 23 kg DM for spring-calving cows and 20 kg DM for autumn-calving cows.European Unio

Active-Coupling Mixing Times for a Stirred Binary Liquid

Mixing times measured for a stirred critical binary liquid mixture are seen to vary dramatically with Reynolds number, Prandtl number, and the initial value of the order parameter. These variations are far too large to be explained by passive-mixing calculations; they also differ in significant respects from the active-mixing predictions of Ruiz and Nelson

Disruption of the Cdc42/Par6/aPKC or Dlg/Scrib/Lgl polarity complex promotes epithelial proliferation via overlapping mechanisms

The establishment and maintenance of apical-basal polarity is a defining characteristic and essential feature of functioning epithelia. Apical-basal polarity (ABP) proteins are also tumor suppressors that are targeted for disruption by oncogenic viruses and are commonly mutated in human carcinomas. Disruption of these ABP proteins is an early event in cancer development that results in increased proliferation and epithelial disorganization through means not fully characterized. Using the proliferating Drosophila melanogaster wing disc epithelium, we demonstrate that disruption of the junctional vs. basal polarity complexes results in increased epithelial proliferation via distinct downstream signaling pathways. Disruption of the basal polarity complex results in JNK-dependent proliferation, while disruption of the junctional complex primarily results in p38-dependent proliferation. Surprisingly, the Rho-Rok-Myosin contractility apparatus appears to play opposite roles in the regulation of the proliferative phenotype based on which polarity complex is disrupted. In contrast, non-autonomous Tumor Necrosis Factor (TNF) signaling appears to suppress the proliferation that results from apical-basal polarity disruption, regardless of which complex is disrupted. Finally we demonstrate that disruption of the junctional polarity complex activates JNK via the Rho-Rok-Myosin contractility apparatus independent of the cortical actin regulator, Moesin

Comparing various multi-component global heliosphere models

Modeling of the global heliosphere seeks to investigate the interaction of the solar wind with the partially ionized local interstellar medium. Models that treat neutral hydrogen self-consistently and in great detail, together with the plasma, but that neglect magnetic fields, constitute a sub-category within global heliospheric models. There are several different modeling strategies used for this sub-category in the literature. Differences and commonalities in the modeling results from different strategies are pointed out. Plasma-only models and fully self-consistent models from four research groups, for which the neutral species is modeled with either one, three, or four fluids, or else kinetically, are run with the same boundary parameters and equations. They are compared to each other with respect to the locations of key heliospheric boundary locations and with respect to the neutral hydrogen content throughout the heliosphere. In many respects, the models' predictions are similar. In particular, the locations of the termination shock agree to within 7% in the nose direction and to within 14% in the downwind direction. The nose locations of the heliopause agree to within 5%. The filtration of neutral hydrogen from the interstellar medium into the inner heliosphere, however, is model dependent, as are other neutral results including the hydrogen wall. These differences are closely linked to the strength of the interstellar bow shock. The comparison also underlines that it is critical to include neutral hydrogen into global heliospheric models.Comment: 10 pages, 4 figures, submitted to a special section at A&A of an ISSI team "Determination of the physical Hydrogen parameters of the LIC from within the Heliosphere

Familial Infiltrative Fibromatosis (Desmoid Tumours) (MIM135290) Caused by a Recurrent 3′ APC Gene Mutation

Desmoid tumours are generally very rare but occur about 100 times more frequently in the colorectal cancer predisposition syndrome familial adenomatous polyposis (MIM 175100), being represented in about 10% of patients. In addition to desmoid disease occurring in familial adenomatous polyposis (FAP) there exist familial infiltrative fibromatosis (MIM 135290) kindreds where there is no evidence of FAP. Previously we have described a kindred with familial infiltrative fibromatosis (FIF) in which desmoid tumours were associated with nonpolyposis colorectal cancer. FAP is caused by mutations in the APC gene and various genotype-phenotype relationships have been defined including reports that colorectal polyposis is less severe with mutations 5′ to codon 157 and that the risk of desmoid tumours is high in FAP patients with APC gene mutations between codons 1444 and 1598. There is relatively little information on the phenotype of APC gene mutations 3′ to codon 1598; however, one large family has been reported with a mutation at codon 1987 which presents with a highly variable phenotype which includes desmoid disease. We screened our original FIF kindred and three further families with a similar phenotype for mutations in the APC gene. A 4 bp frameshift deletion in codon 1962 was identified in the original FIF kindred and two further apparently unrelated families. Haplotype analysis suggests a common origin for the APC mutation in all three families. Affected individuals had no evidence of congenital hypertrophy of the retinal pigment epithelium. Colorectal polyposis was variable, and most affected patients had either none or a few late onset polyps. These findings demonstrate (i) that FAP and FIF are allelic, and (ii) that APC gene mutations which truncate the APC protein distal to the beta-catenin binding domain are associated with desmoid tumours, absent CHRPE and variable but attenuated polyposis expressio

A Decolonial Critique of the Racialized “Localwashing” of Extraction in Central Africa

Responding to calls for increased attention to actions and reactions “from above” within the extractive industry, we offer a decolonial critique of the ways in which corporate entities and multinational institutions propagate racialized rhetoric of “local” suffering, “local” consultation, and “local” fault for failure in extractive zones. Such rhetoric functions to legitimize extractive intervention within a set of practices that we call localwashing. Drawing from a decade of research on and along the Chad-Cameroon Oil Pipeline, we show how multi-scalar actors converged to assert knowledge of, responsibility for, and collaborations with “local” people within a racialized politics of scale. These corporate representations of the racialized “local” are coded through long-standing colonial tropes. We identify three interrelated and overlapping flexian elite rhetoric(s) and practices of racialized localwashing: (a) anguishing, (b) arrogating, and (c) admonishing. These elite representations of a racialized “local” reveal diversionary efforts “from above” to manage public opinion, displace blame for project failures, and domesticate dissent in a context of persistent scrutiny and criticism from international and regional advocates and activists

Fetal central nervous system anomalies: When should we offer exome sequencing?

OBJECTIVE: To investigate the detection of pathogenic variants using exome sequencing in an international cohort of fetuses with central nervous system (CNS) anomalies. METHODS: We reviewed trio exome sequencing (ES) results for two previously reported unselected cohorts (Prenatal Assessment of Genomes and Exomes (PAGE) and CUIMC) to identify fetuses with CNS anomalies with unremarkable karyotypes and chromosomal microarrays. Variants were classified according to ACMG guidelines and association of pathogenic variants with specific types of CNS anomalies explored. RESULTS: ES was performed in 268 pregnancies with a CNS anomaly identified using prenatal ultrasound . Of those with an isolated, single, CNS anomaly, 7/97 (7.2%) had a likely pathogenic/pathogenic (LP/P) variant. This includes 3/23 (13%) fetuses with isolated mild ventriculomegaly and 3/10 (30%) fetuses with isolated agenesis of the corpus callosum. Where there were multiple anomalies within the CNS, 12/63 (19%) had LP/P variants. Of the 108 cases with CNS and other organ system anomalies, 18 (16.7%) had LP/P findings. CONCLUSION: ES is an important tool in the prenatal evaluation of fetuses with any CNS anomaly. The rate of LP/P variants tends to be highest in fetuses with multiple CNS anomalies and multisystem anomalies, however, ES may also be of benefit for isolated CNS anomalies