Limnological changes and chironomid-inferred summer air temperature from the Late Pleniglacial to the Early Holocene in the East Carpathians

Here we provide the first chironomid record and associated summer air-temperature (T VII ) reconstruction between ca. 16,800-9100 cal yr BP from Lake Saint Anne (SZA), situated in the Eastern Carpathians. SZA was formed by the youngest volcanic eruption of Ciomadul volcano at ca. 29,600 cal yr BP. Our main goals in this study are to test whether warming after Heinrich event 1 (H1; ca. 16,200 cal yr BP) had similar amplitude to the late glacial warming, while Younger Dryas (YD) summers remained relatively warm in this region of Europe. We found the most remarkable chironomid assemblage change with a T VII increase of ~3.5-3.8°C at ca. 16,350 cal yr BP at SZA, followed by another slight T VII increase of ~0.8-1.0°C at ca. 14,450 cal yr BP. Only very minor temperature variations were recorded between 14,450 cal yr BP and 11,700 cal yr BP, with an unexpected T VII decrease in the Early Holocene. Variations in water depth together with increasing analogue problems and paludification from ca. 14,200 cal yr BP onwards may have influenced the reliability of our paleotemperature record obtained from SZA. In addition, Sphagnum -indicated decreasing pH, and hence decreasing nutrient level, likely overrode the effect of summer air-temperature changes during the Early Holocene, and this may explain the bias in the chironomid-inferred summer air-temperature reconstruction in the Early Holocene section

Diatom-based evidence for abrupt climate changes during the Late Glacial in the Southern Carpathian Mountains

Abstract A high-resolution paleolimnological record from Lake Brazi (TDB-1; 45°23’47″N, 22°54’06″E, 1740 m a.s.l.), a small, glacial lake in the Retezat (South Carpathian Mountains, Romania) provides a sensitive record of the impacts of late glacial climatic change on siliceous algal assemblages. The sequence, ranging from 15,700 cal yr BP to 9500 cal yr BP, suggests that the most significant changes in diatom assemblages took place at 12,800 and 10,400 cal yr BP, when alkaliphilous fragilarioid taxa were replaced by acidophilous diatoms. Altogether eight zones were distinguished with sharp and rapid changes of diatom assemblages. The paper discusses the application of siliceous algae in multi-proxy paleolimnological analyses, demonstrates the advantages and disadvantages of this proxy and presents the story of floristic discovery of unique diatom assemblages, the closest recent analogs of which are found in the arctic region

Radiocarbon chronology of glacial lake sediments in the Retezat Mts (South Carpathians, Romania): a window to Late Glacial and Holocene climatic and paleoenvironmental changes

Abstract the Retezat Mountains, this study discusses radiocarbon chronology and sediment accumulation rate changes in two sediment profiles in relation to lithostratigraphy, organic content, biogenic silica and major pollenstratigraphic changes. A total of 25 radiocarbon dates were obtained from sediments of two lakes, Lake Brazi (TDB-1; 1740 m a.s.l.) and Lake Gales (Gales-3; 1990 m a.s.l.). Age-depth modeling was performed on TDB-1 using calibrated age ranges from BCal and various curve-fitting methods in psimpoll. Our results suggest that sediment accumulation began between 15,124–15,755 cal yr BP in both lakes and was continuous throughout the Late Glacial and Holocene. We demonstrated that local ecosystem productivity showed delayed response to Late Glacial and Early Holocene climatic changes in the subalpine and alpine zones most likely attributable to the cooling effect of remnant glaciers and meltwater input. However, regional vegetation response was without time lag and indicated forestation and warming at 14,450 and 11,550 cal yr BP, and cooling at ca. 12,800 cal yr BP. In the Holocene one major shift was detected, starting around 6300 cal yr BP and culminating around 5200 cal yr BP. The various proxies suggested summer cooling, shorter duration of the winter ice-cover season and/or increasing size of the water body, probably in response to increasing available moisture

Population dynamics and genetic changes of Picea abies in the South Carpathians revealed by pollen and ancient DNA analyses

Background: Studies on allele length polymorphism designate several glacial refugia for Norway spruce (Picea abies) in the South Carpathian Mountains, but infer only limited expansion from these refugia after the last glaciation. To better understand the genetic dynamics of a South Carpathian spruce lineage, we compared ancient DNA from 10,700 and 11,000-year-old spruce pollen and macrofossils retrieved from Holocene lake sediment in the Retezat Mountains with DNA extracted from extant material from the same site. We used eight primer pairs that amplified short and variable regions of the spruce cpDNA. In addition, from the same lake sediment we obtained a 15,000-years-long pollen accumulation rate (PAR) record for spruce that helped us to infer changes in population size at this site.Results: We obtained successful amplifications for Norway spruce from 17 out of 462 pollen grains tested, while the macrofossil material provided 22 DNA sequences. Two fossil sequences were found to be unique to the ancient material. Population genetic statistics showed higher genetic diversity in the ancient individuals compared to the extant ones. Similarly, statistically significant Ks and Kst values showed a considerable level of differentiation between extant and ancient populations at the same loci.Lateglacial and Holocene PAR values suggested that population size of the ancient population was small, in the range of 1/10 or 1/5 of the extant population. PAR analysis also detected two periods of rapid population growths (from ca. 11,100 and 3900 calibrated years before present (cal yr BP)) and three bottlenecks (around 9180, 7200 and 2200 cal yr BP), likely triggered by climatic change and human impact.Conclusion: Our results suggest that the paternal lineages observed today in the Retezat Mountains persisted at this site at least since the early Holocene. Combination of the results from the genetic and the PAR analyses furthermore suggests that the higher level of genetic variation found in the ancient populations and the loss of ancient allele types detected in the extant individuals were likely due to the repeated bottlenecks during the Holocene; however our limited sample size did not allow us to exclude sampling effect.This study demonstrates how past population size changes inferred from PAR records can be efficiently used in combination with ancient DNA studies. The joint application of palaeoecological and population genetics analyses proved to be a powerful tool to understand the influence of past population demographic changes on the haplotype diversity and genetic composition of forest tree species

Climate and land-use as the main drivers of recent environmental change in a mid-altitude mountain lake, Romanian Carpathians

Recent decades have been marked by unprecendented environmental changes which threaten the integrity of freshwater systems and their ecological value. Although most of these changes can be attributed to human activities, disentagling natural and anthropogenic drivers remains a challenge. In this study, surface sediments from Lake Ighiel, a mid-altitude site in the Carpathian Mts (Romania) were investigated following high-resolution sedimentological, geochemical, environmental magnetic and diatom analyses supported by historical cartographic and documentary evidence. Our results suggest that between 1920 and 1960 the study area experienced no significant anthropogenic impact. An excellent correspondence is observed between lake proxy responses (e.g., growth of submerged macrophytes, high detrital input, shifts in diatom assemblages) and parameters tracking natural hydroclimate variability (e.g., temperature, NAO). This highlights a dominant natural hydroclimatic control on the lacustrine system. From 1960 however, the depositional regime shifted markedly from laminated to homogenous clays; since then geochemical and magnetic data document a trend of significant (and on-going) subsurface erosion across the catchment. This is paralleled by a shift in lake ecosystem conditions denoting a strong response to an intensified anthropogenic impact, mainly through forestry. An increase in detrital input and marked changes in the diatom community are observed over the last three decades, alongside accelerated sedimentation rates following enhanced grazing and deforestation in the catchment. Recent shifts in diatom assemblages may also reflect forcing from atmospheric nitrogen (N) deposition, a key recent drive of diatom community turnover in mountain lakes. In general, enhanced human pressure alongside intermittent hydroclimate forcing drastically altered the landscape around Lake Ighiel and thus, the sedimentation regime and the ecosystem’s health. However, paleoenvironmental signals tracking natural hydroclimate variability are also clearly discernible in the proxy data. Our work illustrates the complex link between the drivers of catchment-scale impacts on one hand, and lake proxy responses on the other, highlighting the importance of an integrated historical and palaeolimnological approach to better assess lake system changes

Limnological changes in South Carpathian glacier-formed lakes (Retezat Mountains, Romania) during the Late Glacial and the Holocene: A synthesis

Remains of aquatic biota preserved in mountain lake sediments provide an excellent tool to study lake ecosystem responses to past climate change. In the PROLONG project a multi-proxy study was performed on sediments of glacier-formed lakes from the Retezat Mountains, Southern Carpathians (Romania). The studied lakes (Lake Brazi and Gales) are situated on the northern slope of the mountain at different altitudes (1740 m and 1990 m a.s.l.). Our main objectives were 1) to describe the main limnological changes in these lakes during the last ca. 15,000 years and 2) to summarize the environmental history of the studied lakes based on taxonomical and functional patterns of the biological proxies. For this synthesis we used the results of diatom and chironomid analyses, and indirect biotic and abiotic parameters, including sediment organic matter (LOI) content, geochemical element concentrations (Al, Ca, S, Sr) and biogenic silica content. Using multivariate numerical approaches we analysed changes in the assemblage structure of siliceous algae and chironomids, compared temporal patterns among proxies, examined the relationship between potential driving factors, chironomid and diatom assemblage changes and identified paleolimnological phases of the lake successions. Changes in assemblage composition and aquatic ecosystem state apparently followed summer insolation, local climatic conditions and local productivity changes driven by these. Diatom and chironomid assemblages generally changed in a similar direction and at a similar time within a lake, but differed to some extent between Lake Brazi and Gales. At both lakes the strongest variations were observed in the Late Glacial and the first half of the Holocene. The strongest Holocene assemblage changes took place in the earliest Holocene in Lake Brazi, but extended into the mid-Holocene in Lake Gales, following long-term insolation changes and climatic changes. In addition, three common zone boundaries were identified: at ca. 14,200 and at ca. 6500 cal yr BP for every records and at ca. 3100 cal yr BP for diatom records in both of the lakes and for the chironomid record of Lake Brazi. This multi-proxy synthesis provides comprehensive data that increase our understanding of the past variability of lake ecosystem functioning and biodiversity in East-Central Europe. Keyword

Effect of Temperature on the Size of Sedimentary Remains of Littoral Chydorids

The body size of aquatic invertebrates is, to a great extent, dependent on ambient temperature, but size distributions are also determined by other factors like food supply and predation. The effect of temperature on organisms is formulated in the temperature–size hypothesis, which predicts a smaller body size with increasing temperature. In this study, the effect of temperature on the subfossil remains of three littoral Cladocera (Alona affnis, A. quadrangularis, and Chydorus cf. sphaericus) was investigated. Exoskeletal remains of these species can be found in large numbers in lacustrine sediments and over a wide north–south range in Europe. The total length of both headshield and postabdomen for A. affinis and A. quadrangularis and carapace length for C. cf. sphaericus were measured to observe their response to changes in latitude and temperature. A different response to ambient temperature in the growth of body parts was observed. The size of the headshields of both Alona species and of the carapace of Chydorus was significantly larger in colder regions as opposed to warm ones. It turned out that the postabdomen was not a good predictor of ambient temperature. While the sizes of all remains increased with latitude, the sizes of the Alona remains was smaller in the mountain lakes of the Southern Carpathians than in other cold lakes, in this case in Finland, a fact indicative of the importance of other factors on size distribution. This study demonstrates that a morphological response to climate is present in littoral cladocerans, and, therefore, changes in the length of headshield and carapace may be used as a proxy for climate changes in paleolimnological records

Exceptionally well-preserved giant spermatozoa in male and female specimens of an ostracod Cypria ophtalmica (Crustacea: Ostracoda) from Late Glacial lacustrine sediments of Southern Carpathians, Romania

Exceptionally well-preserved giant spermatozoa observed between abundant decalcified carapace valves of ostracods (Crustacea: Ostracoda) were found in Late Glacial to Holocene (14,400 to 10,000 cal years BP) lacustrine sediments in the southern Romanian Carpathians. Analysis by scanning electron microscopy and laser scanning confocal microscopy revealed good preservation of the appendages enabling specific identification as Cypria ophtalmica (Candonidae) and indication of the presence of both female and male specimens based on the sexual dimorphism of the second antenna. This record represents the oldest and richest direct evidence of virtually morphologically unaltered animal spermatozoa preserved in females after mating

Ciomadul (Csomád), The Youngest Volcano in the Carpathians Volcanism, Palaeoenvironment, Human Impact

This book delivers the present state-of-the-art of scientific characteristics of the unique Ciomadul volcano (Romania, East-Central Europe) from as many aspects as possible.. Multidisciplinary research results obtained on this geologically young volcanic complex are presented to a wider audience (geologists, volcanologists, botanists, archaeologists, historians and teachers). Moreover, the book provides information at a general level for interested laypersons and decision-makers. The first part of the book, after summarizing the research history of Ciomadul, presents the details of the volcanism and related topics (volcanology, geology, landscape evolution, minerals, post-volcanic activity and spa culture) in eight chapters; the second part deals with the palaeo-environmental issues of the larger area, along with human history, in nine chapters

Plant macrofossils from lake sediment as the material to assess ancient genetic diversity: Did deforestation influence Norway spruce ( Picea abies ) in the South Carpathians?

How genetic diversity of populations reacts to neutral or adaptive processes such as population bottlenecks, immigration or local adaptation are central questions of population genetics. They may be directly answered through ancient DNA analysis, however such studies in plants are remarkably scarce, owing to the difficulty of gaining population-scale samples with good DNA preservation. Plant macrofossils are abundant in lake sediments, and here we assessed if they can be valuable material for population genetic studies. We extracted ancient DNA from early to late Holocene seed and needle remains of Norway spruce (Picea abies L. Karst) recovered from the sediment of a lake in the Retezat Mts. (South Carpathians). We aimed to reveal whether deforestations starting in the late Holocene and reaching maximum in the 19th and early 20th centuries have caused decreased genetic diversity in the modern Norway spruce population of the Retezat Mts. In a combined approach, we sequenced two plastid regions and generated detailed paleobotanical data to reconstruct the population history of Norway spruce. Our results show that genotyping success of the ancient material was high in samples younger than c. 2000-year-old, but was very low in early Holocene samples, and needles allowed genotyping with higher success compared to seeds. We discovered macrofossil samples that contained DNA from more than a single Norway spruce individual. This phenomenon, together with the possible multiple sampling of the same individual needs to be kept in mind when interpreting the genetic data obtained from macrofossils. Our results overall indicate a genetic diversity decrease during the late Holocene. We demonstrate that macrofossils preserved in sediments are invaluable material for population genetic studies