Evidence of Unprecedented High Electronic Conductivity in Mammalian Pigment Based Eumelanin Thin Films After Thermal Annealing in Vacuum

Melanin denotes a variety of mammalian pigments, including the dark electrically conductive eumelanin and the reddish, sulfur-containing, pheomelanin. Organic (bio)electronics is showing increasing interests in eumelanin exploitation, e.g., for bio-interfaces, but the low conductivity of the material is limiting the development of eumelanin-based devices. Here, for the first time, we report an abrupt increase of the eumelanin electrical conductivity, revealing the highest value presented to date of 318 S/cm. This result, obtained via simple thermal annealing in vacuum of the material, designed on the base of the knowledge of the eumelanin chemical properties, also discloses the actual electronic nature of this material's conduction

Una propuesta problematizadora en la formación docente del Profesorado Universitario de Educación Física de la Universidad de Luján

La presente ponencia sintetiza la propuesta de enseñanza que desarrollamos como equipo docente en el ámbito de la formación docente en Educación Física dentro de la Universidad Nacional de Luján. El trabajo desarrollado se ancla en el doble propósito que traza la asignatura consistente en brindar algunas herramientas a los estudiantes ingresantes para acercarse a la reflexión sobre la enseñanza y, a su vez, a las características del estudio universitario. Asumimos esta tarea desde una perspectiva crítica y problematizadora de la educación, haciendo consciente la dimensión éticopolítica de la enseñanza y buscando lograr una coherencia entre las intervenciones como equipo y la búsqueda por la transformación social. Tarea que no está exenta de debates ni dificultades pero que supone una constante reflexión sobre lo que se hace para garantizar a nuestrxs estudiantes la apropiación de conocimientos y prácticas ligadas al compromiso con lxs otrxs.Mesa 16: Educación física y educación superiorFacultad de Humanidades y Ciencias de la Educació

El diseño curricular del PUEF-UNLu: entre el conocimiento disciplinar y el conocimiento didáctico del contenido para la formación de profesores en Educación Física

En el marco de un proyecto de investigación del Equipo Docente de la asignatura de Teorías de la Enseñanza y el currículo dentro del Profesorado Universitario en Educación Física comenzamos a intentar comprender y describir de qué modo se articulan en la actualidad los conocimientos didácticos del contenido y los conocimientos disciplinares en el Plan de Estudios del Profesorado Universitario de Educación Física de la Universidad Nacional de Luján (PUEF-UNLu). De este modo, dicha problemática gira en torno a algunos interrogantes clave: ¿De qué manera queda plasmado en el texto curricular la relación entre los diferentes tipos de conocimientos propios de los campos de saber que conforman la Educación Física para la Formación Docente? ¿Existe un predominio de conocimientos disciplinares por sobre el conocimientos didácticos del contenido? ¿Qué tensiones surgen en esta articulación? ¿Es posible dar cuenta de la permanencia de una tradición consolidada en la Formación Docente a nivel del Plan de Estudios? ¿Qué implicancias podría tener esto para el desarrollo curricular de las asignaturas?Facultad de Humanidades y Ciencias de la Educació

Identification of host cell factors required for intoxication through use of modified cholera toxin

We describe a novel labeling strategy to site-specifically attach fluorophores, biotin, and proteins to the C terminus of the A1 subunit (CTA1) of cholera toxin (CTx) in an otherwise correctly assembled and active CTx complex. Using a biotinylated N-linked glycosylation reporter peptide attached to CTA1, we provide direct evidence that ∼12% of the internalized CTA1 pool reaches the ER. We also explored the sortase labeling method to attach the catalytic subunit of diphtheria toxin as a toxic warhead to CTA1, thus converting CTx into a cytolethal toxin. This new toxin conjugate enabled us to conduct a genetic screen in human cells, which identified ST3GAL5, SLC35A2, B3GALT4, UGCG, and ELF4 as genes essential for CTx intoxication. The first four encode proteins involved in the synthesis of gangliosides, which are known receptors for CTx. Identification and isolation of the ST3GAL5 and SLC35A2 mutant clonal cells uncover a previously unappreciated differential contribution of gangliosides to intoxication by CTx.Fundação para a Ciência e a Tecnologia (Fellowship

Clinical Features, Cardiovascular Risk Profile, and Therapeutic Trajectories of Patients with Type 2 Diabetes Candidate for Oral Semaglutide Therapy in the Italian Specialist Care

Introduction: This study aimed to address therapeutic inertia in the management of type 2 diabetes (T2D) by investigating the potential of early treatment with oral semaglutide. Methods: A cross-sectional survey was conducted between October 2021 and April 2022 among specialists treating individuals with T2D. A scientific committee designed a data collection form covering demographics, cardiovascular risk, glucose control metrics, ongoing therapies, and physician judgments on treatment appropriateness. Participants completed anonymous patient questionnaires reflecting routine clinical encounters. The preferred therapeutic regimen for each patient was also identified. Results: The analysis was conducted on 4449 patients initiating oral semaglutide. The population had a relatively short disease duration (42%  60% of patients, and more often than sitagliptin or empagliflozin. Conclusion: The study supports the potential of early implementation of oral semaglutide as a strategy to overcome therapeutic inertia and enhance T2D management

Robotic gait training in patients with cerebral palsy: a retrospective analysis

Objetivo: Verificar retrospectivamente a eficácia do treino de marcha com robótica (Lokomat®) em pacientes com Paralisia Cerebral (PC) níveis II, III e IV da Medida da Função Motora Grossa (GMFCS). Método: Análise retrospectiva descritiva do prontuário de 69 pacientes com PC que realizaram o protocolo da Lokomat®. Os resultados do teste de caminhada de 6 minutos (TC6M), teste de caminhada de 10 metros (TC10M), Timed Up and Go (TUG) e da Avaliação da Função Motora Grossa (GMFM) foram realizados e analisados pré e pós protocolo. Resultados: Foi observada uma melhora no GMFM para pacientes de todos os níveis do GMFCS. Pacientes GMFCS nível III apresentaram melhora significativa do TC6M (p= 0,01) e pacientes GMFCS nível IV apresentaram melhora significativa da dimensão B do GMFM (p= 0,03). Todos os testes mostraram melhoras significativas quando comparados aos resultados antes da aplicação do protocolo. Conclusão: O estudo sugere que todos os pacientes com diagnóstico de PC se beneficiaram do treino de marcha com uso da Lokomat® dentro de seu quadro motor esperado.Objective: The present study sought to retrospectively evaluate the efficacy of robotic gait training in children with cerebral palsy (CP) gross motor function classification system (GMFCS) levels II, III and IV. Method: The medical records from 69 patients with CP, who participated in the Lokomat® protocol, were analyzed using a retrospective approach. The results from the Gross Motor Function Measure (GMFM), 6-minute walk test (6MinWT), 10-meter walk test (10MWT) and Timed Up and Go (TUG) test were analyzed before and after the protocol was administered. Results:  An improvement in GMFM was observed for all GMFCS levels. GMFCS level III patients showed a significant improvement in the 6MinWT (p= 0.01), and GMFCS level IV patients displayed a significant improvement in GMFM dimension B (p= 0.03). All tests showed a significant improvement when compared to their performance before the application of the protocol. Conclusion: The study suggests that all patients diagnosed with CP benefit from gait training, using the Lokomat® system, within their expected motor frame

ACID BASE CHARACTERIZATION OF TRIAZOLO-TRIAZOLE HETEROCYCLIC SYSTEMS

Heterocycles are key compounds of synthetic chemistry. Besides their long standing and relevant application as drugs and bioactive compounds, aromatic heterocycles are playing a fundamental role in modern material chemistry as building blocks of conjugated active molecules in many emerging fields of organic electronics and optoelectronics: conducting polymers, organic field-effect transistors,[1] organic solar cells,[2] nonlinear optically active compounds.[3] In this work we propose a study of acid-base properties of four [1,2,4]triazolo[3,2-c][1,2,4]triazole systems[4]. The experimental method elected consists of potentiometric, spectrophotometric titrations, in constant ionic medium NaCl 0.5M at 25.00±0.02°C. Electronic absorption spectra have been interpreted with the following equilibria: These results shown that the acid-base properties of [1,2,4]triazolo[3,2- c][1,2,4]triazole derivatives critically depends on the substituents at the heterobicycle. [1] J. Zaumseil, H. Sirringhaus, Chem. Rev., 2007, 107, 1296-1323; b) Y. Wen, Y. Liu, Adv. Mater., 2010, 22,1-15. [2] J. Li, A. C. Grimsdale, Chem. Soc. Rev. 2010, 39, 2399-2410. [3] S. H. Jang, A. K. Jen, Introduction to Organic Electronic and Optoelectronic Materials and Devices (Eds. S. S. Sun, L. R. Dalton) CRC Press, Boca Raton (FL), 2008. [4] R. Centore, S. Fusco, A. Capobianco, V. Piccialli, S. Zaccaria, and A. Peluso, European Journal of Organic Chemistry, 2013, 18, 3721-37