227 research outputs found
Population studies of the desert cottontail (Sylvilagus auduboni) and black-tailed jackrabbit (Lepus californicus) in the Sonoran Desert
Novel monoclonal antibodies against Pdx1 reveal feedback regulation of Pdx1 protein levels
The aim of this study was to characterize two monoclonal antibodies (F6A11 and F109-D12) generated against Pdx1 (pancreatic and duodenal homeobox-1), a homeodomain transcription factor, which is critical for pancreas formation as well as for normal pancreatic beta cell function. For production of monoclonal antibodies, we immunized Robertsonian POSF (RBF)mice with a GST-Pdx1 fusion protein containing a 68-amino acid C-terminal fragment of rat Pdx1. These monoclonal antibodies detect Pdx1 by western blotting and allow immunohistochemical detection of Pdx1 in both mouse and rat tissue. F6A11 and F109-D12 produce IHC staining patterns indistinguishable from that obtained with highly specific polyclonal Pdx1 antisera raised in rabbits and goats, when applied to embryonic or adult mouse pancreatic tissue. In contrast to previously generated polyclonal anti-Pdx1 antisera, we also demonstrate that F6A11 works for intracellular fluorescence activated cell sorting (FACS) staining of Pdx1. By using F6A11, we characterize the induction of Pdx1 in the Doxycycline (DOX) inducible insulinoma cell line INSrαÎČ-Pdx1 and follow the reduction of Pdx1 after removing Dox. Finally, we show that induction of exogenous Pdx1 leads to a reduction in endogenous Pdx1 levels, which suggests that a negative feedback loop is involved in maintaining correct levels of Pdx1 in the cell
Photoionization of ultracold and Bose-Einstein condensed Rb atoms
Photoionization of a cold atomic sample offers intriguing possibilities to
observe collective effects at extremely low temperatures. Irradiation of a
rubidium condensate and of cold rubidium atoms within a magneto-optical trap
with laser pulses ionizing through 1-photon and 2-photon absorption processes
has been performed. Losses and modifications in the density profile of the
remaining trapped cold cloud or the remaining condensate sample have been
examined as function of the ionizing laser parameters. Ionization
cross-sections were measured for atoms in a MOT, while in magnetic traps losses
larger than those expected for ionization process were measured.Comment: 9 pages, 7 figure
On Higher Order Gravities, Their Analogy to GR, and Dimensional Dependent Version of Duff's Trace Anomaly Relation
An almost brief, though lengthy, review introduction about the long history
of higher order gravities and their applications, as employed in the
literature, is provided. We review the analogous procedure between higher order
gravities and GR, as described in our previous works, in order to highlight its
important achievements. Amongst which are presentation of an easy
classification of higher order Lagrangians and its employment as a
\emph{criteria} in order to distinguish correct metric theories of gravity. For
example, it does not permit the inclusion of only one of the second order
Lagrangians in \emph{isolation}. But, it does allow the inclusion of the
cosmological term. We also discuss on the compatibility of our procedure and
the Mach idea. We derive a dimensional dependent version of Duff's trace
anomaly relation, which in \emph{four}-dimension is the same as the usual Duff
relation. The Lanczos Lagrangian satisfies this new constraint in \emph{any}
dimension. The square of the Weyl tensor identically satisfies it independent
of dimension, however, this Lagrangian satisfies the previous relation only in
three and four dimensions.Comment: 30 pages, added reference
On Physical Equivalence between Nonlinear Gravity Theories
We argue that in a nonlinear gravity theory, which according to well-known
results is dynamically equivalent to a self-gravitating scalar field in General
Relativity, the true physical variables are exactly those which describe the
equivalent general-relativistic model (these variables are known as Einstein
frame). Whenever such variables cannot be defined, there are strong indications
that the original theory is unphysical. We explicitly show how to map, in the
presence of matter, the Jordan frame to the Einstein one and backwards. We
study energetics for asymptotically flat solutions. This is based on the
second-order dynamics obtained, without changing the metric, by the use of a
Helmholtz Lagrangian. We prove for a large class of these Lagrangians that the
ADM energy is positive for solutions close to flat space. The proof of this
Positive Energy Theorem relies on the existence of the Einstein frame, since in
the (Helmholtz--)Jordan frame the Dominant Energy Condition does not hold and
the field variables are unrelated to the total energy of the system.Comment: 37 pp., TO-JLL-P 3/93 Dec 199
Muon Track Reconstruction and Data Selection Techniques in AMANDA
The Antarctic Muon And Neutrino Detector Array (AMANDA) is a high-energy
neutrino telescope operating at the geographic South Pole. It is a lattice of
photo-multiplier tubes buried deep in the polar ice between 1500m and 2000m.
The primary goal of this detector is to discover astrophysical sources of high
energy neutrinos. A high-energy muon neutrino coming through the earth from the
Northern Hemisphere can be identified by the secondary muon moving upward
through the detector. The muon tracks are reconstructed with a maximum
likelihood method. It models the arrival times and amplitudes of Cherenkov
photons registered by the photo-multipliers. This paper describes the different
methods of reconstruction, which have been successfully implemented within
AMANDA. Strategies for optimizing the reconstruction performance and rejecting
background are presented. For a typical analysis procedure the direction of
tracks are reconstructed with about 2 degree accuracy.Comment: 40 pages, 16 Postscript figures, uses elsart.st
Results from the Antarctic Muon and Neutrino Detector Array (AMANDA)
We show new results from both the older and newer incarnations of AMANDA
(AMANDA-B10 and AMANDA-II, respectively). These results demonstrate that AMANDA
is a functioning, multipurpose detector with significant physics and
astrophysics reach. They include a new higher-statistics measurement of the
atmospheric muon neutrino flux and preliminary results from searches for a
variety of sources of ultrahigh energy neutrinos: generic point sources,
gamma-ray bursters and diffuse sources producing muons in the detector, and
diffuse sources producing electromagnetic or hadronic showers in or near the
detector.Comment: Invited talk at the XXth International Conference on Neutrino Physics
and Astrophysics (Neutrino 2002), Munich, Germany, May 25-30, 200
Sensitivity of the IceCube Detector to Astrophysical Sources of High Energy Muon Neutrinos
We present the results of a Monte-Carlo study of the sensitivity of the
planned IceCube detector to predicted fluxes of muon neutrinos at TeV to PeV
energies. A complete simulation of the detector and data analysis is used to
study the detector's capability to search for muon neutrinos from sources such
as active galaxies and gamma-ray bursts. We study the effective area and the
angular resolution of the detector as a function of muon energy and angle of
incidence. We present detailed calculations of the sensitivity of the detector
to both diffuse and pointlike neutrino emissions, including an assessment of
the sensitivity to neutrinos detected in coincidence with gamma-ray burst
observations. After three years of datataking, IceCube will have been able to
detect a point source flux of E^2*dN/dE = 7*10^-9 cm^-2s^-1GeV at a 5-sigma
significance, or, in the absence of a signal, place a 90% c.l. limit at a level
E^2*dN/dE = 2*10^-9 cm^-2s^-1GeV. A diffuse E-2 flux would be detectable at a
minimum strength of E^2*dN/dE = 1*10^-8 cm^-2s^-1sr^-1GeV. A gamma-ray burst
model following the formulation of Waxman and Bahcall would result in a 5-sigma
effect after the observation of 200 bursts in coincidence with satellite
observations of the gamma-rays.Comment: 33 pages, 13 figures, 6 table
On the selection of AGN neutrino source candidates for a source stacking analysis with neutrino telescopes
The sensitivity of a search for sources of TeV neutrinos can be improved by
grouping potential sources together into generic classes in a procedure that is
known as source stacking. In this paper, we define catalogs of Active Galactic
Nuclei (AGN) and use them to perform a source stacking analysis. The grouping
of AGN into classes is done in two steps: first, AGN classes are defined, then,
sources to be stacked are selected assuming that a potential neutrino flux is
linearly correlated with the photon luminosity in a certain energy band (radio,
IR, optical, keV, GeV, TeV). Lacking any secure detailed knowledge on neutrino
production in AGN, this correlation is motivated by hadronic AGN models, as
briefly reviewed in this paper.
The source stacking search for neutrinos from generic AGN classes is
illustrated using the data collected by the AMANDA-II high energy neutrino
detector during the year 2000. No significant excess for any of the suggested
groups was found.Comment: 43 pages, 12 figures, accepted by Astroparticle Physic
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