Cloning and expression of a novel human profilin variant, profilin II

AbstractWe have isolated a 1.7 kbp cDNA encoding a 140 amino acid protein (15.1 kDa, pI 5.91) with a high sequence similarity (62%) to human profilin (profilin I). We have termed this variant profilin II. Northern blot analysis showed that profilin II is highly expressed in brain, skeletal muscle and kidney and less strongly in heart, placenta, lung and liver. In addition, three different transcript lengths were detected. Only one transcript of profilin I was found. The expression level of this was low in brain and skeletal muscle, medium in heart and high in placenta, lung, liver and kidney

Monitoring Program for Pharmaceuticals, illegal substances and contaminants in farmed fish - Annual report for 2019

This report summarises the monitoring data collected in 2019 on the status of illegal substances, pharmaceuticals and contaminants in Norwegian farmed fish. A total of 13 725 fish were collected. Samples examined for illegal compounds would be collected at all stages of farming and are representative of farmed fish under production. The samples were analysed for substances with anabolic effects or unauthorized veterinary drugs. No residues of illegal compounds were detected. Samples tested for approved veterinary drugs and contaminants were collected at processing plants and are representative of Norwegian farmed fish ready for human consumption. Residues of anti-sea-lice agents were found in five samples, the levels present were below the Maximum Residue Limit (MRL) for all samples. Other veterinary drugs, like antibiotics or drugs used against internal parasites were not found. No environmental contaminants were found above the EU maximum levels.publishedVersio

The Self-help Online against Suicidal thoughts (SOS) trial:study protocol for a randomized controlled trial

SPIRIT Checklist. (DOC 121 kb

Refined mackerel oil increases hepatic lipid accumulation and reduces choline and choline-containing metabolites in the liver tissue in mice fed a Western diet

In this study, we aimed to evaluate the impact of consuming refined mackerel oil (MO) from rest raw material on hepatic fat accumulation, glucose tolerance, and metabolomic changes in the liver from male C57BL/6N mice. The mice were fed either a Western diet (WD) or a chow diet, with 30 g or 60 g MO per kg of diet (3% or 6%) for 13 weeks. Body weight, energy intake, and feed efficiency were monitored throughout the experiment. A glucose tolerance test was conducted after 11 weeks, and metabolomic analyses of the liver were performed at termination.publishedVersio

Monitoring program for pharmaceuticals, illegal substances, and contaminants in farmed fish - Annual report for 2018

This report summarises the monitoring data collected in 2018 on the status of illegal substances, pharmaceuticals and contaminants in Norwegian farmed fish. A total of 13 920 fish were collected. Samples examined for illegal compounds could be collected at all stages of farming and are representative of farmed fish under production. The samples were analysed for substances with anabolic effects or unauthorized substances. No residues of illegal compounds were detected. Samples tested for approved veterinary drugs and contaminants were collected at processing plants, and are representative of Norwegian farmed fish ready for human consumption. Residues of anti sea lice agents were found in four samples, the levels present were below the Maximum Residue Limit (MRL) for all samples. Other veterinary drugs, like antibiotics or drugs used against internal parasites were not found. No environmental contaminants were found above the EU maximum limits.publishedVersio

Effects of frozen storage on phospholipid content in atlantic cod fillets and the influence on diet-induced obesity in mice

publishedVersio

Dietary selenomethionine reduce mercury tissue levels and modulate methylmercury induced proteomic and transcriptomic alterations in hippocampi of adolescent BALB/c mice

Methylmercury (MeHg) is a well-known environmental contaminant, particularly harmful to the developing brain. The main human dietary exposure to MeHg occurs through seafood consumption. However, seafood also contains several nutrients, including selenium, which has been shown to interact with MeHg and potentially ameliorate its toxicity. The aim of this study was to investigate the combined effects of selenium (as selenomethionine; SeMet) and MeHg on mercury accumulation in tissues and the effects concomitant dietary exposure of these compounds exert on the hippocampal proteome and transcriptome in mice. Adolescent male BALB/c mice were exposed to SeMet and two different doses of MeHg through their diet for 11 weeks. Organs, including the brain, were sampled for mercury analyses. Hippocampi were collected and analyzed using proteomics and transcriptomics followed by multi-omics bioinformatics data analysis. The dietary presence of SeMet reduced the amount of mercury in several organs, including the brain. Proteomic and RNA-seq analyses showed that both protein and RNA expression patterns were inversely regulated in mice receiving SeMet together with MeHg compared to MeHg alone. Several pathways, proteins and RNA transcripts involved in conditions such as immune responses and inflammation, oxidative stress, cell plasticity and Alzheimer’s disease were affected inversely by SeMet and MeHg, indicating that SeMet can ameliorate several toxic effects of MeHg in mice.publishedVersio

Assessment of Suicide Risks During the First Week Immediately After Discharge From Psychiatric Inpatient Facility

Background: The suicide rate in first week after psychiatric discharge is alarmingly high. Although a risk assessment prior to discharge is standard praxis, it can be difficult to take into consideration the obstacles that patient will meet once discharged. A follow-up-visit during the first week after discharge is an opportunity to reevaluate whether a person may be at risk of suicide. Aim: To determine how many patients, of those who were assessed, were evaluated to be at elevated risk of suicide during the first week after psychiatric discharge and secondarily to identify predictors of this and predictors for receiving a follow-up visit during first week after discharge. Methods: All patients discharged between March 1st 2018 to January 17th 2019 were offered a home visit including a systematic risk assessment. Socio-demographics and clinical variables were obtained from medical records and logistic regression analyses were used to identify predictors of a higher suicide risk assessment as well as receiving a follow-up visit. Results: Information from 1905 discharges were included. Of these, 1,052 were seen in follow-up meetings. Risk assessments was conducted in a total of 567 discharge procedures, of which 28 (5%) had an elevated risk of suicide. A history of suicide attempt, suicide risk having been the reason for admission, a first diagnosis of a psychiatric disorder was associated with an elevated risk of suicide after discharge. Conclusion: Follow-up visits could serve as an important tool to identify people whose suicidal risk were overlooked at discharge or exposed to severe stressors after discharge.Helsefonden (grant number: 18-B-0302