An appreciative view of year 9 student leadership in a New Zealand secondary school context

Engaging students in relevant and meaningful leadership activities in schools can prove to be a challenging task. Understanding the leadership experiences and skills that students have is an important beginning step to understanding how schools can best serve their needs. This thesis shares the details of a qualitative research project, which explored year 9 students’ perspectives and experiences of leadership. The study engaged a strengths-based approach to learning and, amongst other things, encouraged students to reflect upon past experiences of leadership. Central to the research design were the voices of the students as they examined these past experiences to find out what factors made leadership enjoyable, meaningful and useful to them. The findings indicated the significant difference in opportunities students were provided in year 8 (intermediate school) as compared with year 9 (secondary school). Coming from environments where students most often had significant leadership opportunities, upon entering high school students perceived few opportunities for leadership. Students shared their views about ways they could contribute to and be involved in leadership at secondary school and suggested changes that needed to occur for this to happen. The findings also highlighted the powerful influence of the school and family on students’ leadership understandings and demonstrated that leadership development for young people should not be something schools ‘do’ to them but something that encourages them to recognise their existing skills and future potential

Modelling the sun's small scale global photospheric magnetic field

We present a new model for the Sun's global photospheric magnetic field during a deep minimum of activity, in which no active regions emerge. The emergence and subsequent evolution of small- scale magnetic features across the full solar surface is simulated, subject to the influence of a global supergranular flow pattern. Visually, the resulting simulated magnetograms reproduce the typical structure and scale observed in quiet Sun magnetograms. Quantitatively, the simulation quickly reaches a steady state, resulting in a mean field and flux distribution that are in good agreement with those determined from observations. A potential coronal magnetic field is extrapolated from the simulated full Sun magnetograms, to consider the implications of such a quiet photospheric magnetic field on the corona and inner heliosphere. The bulk of the coronal magnetic field closes very low down, in short connections between small-scale features in the simulated magnetic network. Just 0.1% of the photospheric magnetic flux is found to be open at 2:5 Rʘ, around 10 - 100 times less than that determined for typical HMI synoptic map observations. If such conditions were to exist on the Sun, this would lead to a significantly weaker interplanetary magnetic field than is presently observed, and hence a much higher cosmic ray flux at Earth

Aspergillus fumigatus enhances elastase production in pseudomonas aeruginosaco-cultures

In the cystic fibrosis (CF) lung the presence of bacteria and fungi in the airways promotes an inflammatory response causing progressive lung damage, ultimately leading to high rates of morbidity and mortality. We hypothesized that polymicrobial interactions play an important role in promoting airway pathogenesis. We therefore examined the interplay between the most commonly isolated bacterial CF pathogen, Pseudomonas aeruginosa, and the most prevalent filamentous fungi, Aspergillus fumigatus, to test this. Co-culture experiments showed that in the presence of A. fumigatus the production of P. aeruginosa elastase was enhanced. This was confirmed by the presence of zones of clearance on Elastin-Congo Red (ECR) agar, which was identified as elastase by mass spectrometry. When P. aeruginosa were grown in a co-culture model with mature A. fumigatus biofilms, 60% of isolates produced significantly more elastase in the presence of the filamentous fungi than in its absence (P < .05). The expression of lasB also increased when P. aeruginosa isolates PA01 and PA14 were grown in co-culture with A. fumigatus. Supernatants from co-culture experiments were also significantly toxic to a human lung epithelial cell line (19–38% cell cytotoxicity) in comparison to supernatants from P. aeruginosa only cultures (P < .0001). Here we report that P. aeruginosa cytotoxic elastase is enhanced in the presence of the filamentous fungi A. fumigatus, suggesting that this may have a role to play in the damaging pathology associated with the lung tissue in this disease. This indicates that patients who have a co-colonisation with these two organisms may have a poorer prognosis

Serine, but not glycine, supports one-carbon metabolism and proliferation of cancer cells

Previous work has shown that some cancer cells are highly dependent on serine/glycine uptake for proliferation. Although serine and glycine can be interconverted and either might be used for nucleotide synthesis and one-carbon metabolism, we show that exogenous glycine cannot replace serine to support cancer cell proliferation. Cancer cells selectively consumed exogenous serine, which was converted to intracellular glycine and one-carbon units for building nucleotides. Restriction of exogenous glycine or depletion of the glycine cleavage system did not impede proliferation. In the absence of serine, uptake of exogenous glycine was unable to support nucleotide synthesis. Indeed, higher concentrations of glycine inhibited proliferation. Under these conditions, glycine was converted to serine, a reaction that would deplete the one-carbon pool. Providing one-carbon units by adding formate rescued nucleotide synthesis and growth of glycine-fed cells. We conclude that nucleotide synthesis and cancer cell proliferation are supported by serine—rather than glycine—consumption

Very small deletions within the NESP55 gene in pseudohypoparathyroidism type 1b

Pseudohypoparathyroidism (PHP) is caused by reduced expression of genes within the GNAS cluster, resulting in parathormone resistance. The cluster contains multiple imprinted transcripts, including the stimulatory G protein α subunit (Gs-α) and NESP55 transcript preferentially expressed from the maternal allele, and the paternally expressed XLas, A/B and antisense transcripts. PHP1b can be caused by loss of imprinting affecting GNAS A/B alone (associated with STX16 deletion), or the entire GNAS cluster (associated with deletions of NESP55 in a minority of cases). We performed targeted genomic next-generation sequencing (NGS) of the GNAS cluster to seek variants and indels underlying PHP1b. Seven patients were sequenced by hybridisation-based capture and fourteen more by long-range PCR and transposon-mediated insertion and sequencing. A bioinformatic pipeline was developed for variant and indel detection. In one family with two affected siblings, and in a second family with a single affected individual, we detected maternally inherited deletions of 40 and 33 bp, respectively, within the deletion previously reported in rare families with PHP1b. All three affected individuals presented with atypically severe PHP1b; interestingly, the unaffected mother in one family had the detected deletion on her maternally inherited allele. Targeted NGS can reveal sequence changes undetectable by current diagnostic methods. Identification of genetic mutations underlying epigenetic changes can facilitate accurate diagnosis and counselling, and potentially highlight genetic elements critical for normal imprint settin

Hormone replacement therapy for women with type 1 diabetes mellitus

Background: There is conflicting information about the impact of the menopause on glycaemic control amongst women with type 1 diabetes. Some menopausal women with type 1 diabetes are treated with hormone replacement therapy (HRT) but the effects of this treatment have, to date, not been established.Objectives: To assess the effects of HRT for women with type 1 diabetes mellitus.Search methods: We searched The Cochrane Library, MEDLINE, EMBASE, CINAHL and PsycINFO from their inception to June 2012. The last search was run for all databases on 18 June 2012.Selection criteria: We selected randomised controlled trials or controlled clinical trials that involved peri‐ or postmenopausal women with type 1 diabetes undergoing HRT as an intervention.Data collection and analysis: Two researchers independently applied the inclusion criteria to the identified studies and assessed risk of bias. Disagreements were resolved by discussion or by intervention by a third party. Descriptive analysis was conducted for the review.Main results: Ninety‐two publications were screened. No studies met the inclusion criteria exclusively but one study that included both type 1 and type 2 diabetes participants was considered. This randomised clinical trial (RCT) compared HRT (N = 27) with placebo (N = 29) over 12 months. The outcome measures were cardiovascular risk factors, including lipid profile, glycaemic control, blood pressure and body weight. No significant differences between placebo and HTR were detected. Patient‐important outcomes like all‐cause mortality, cardiovascular disease, diabetic complications or health‐related quality of life were not investigated.Authors' conclusions: There is a lack of evidence around the use of HRT in women with type 1 diabetes. The one study that has been undertaken in this area is underpowered. More RCTs are required in the area to examine the impact of HRT on glycaemic control and cardiovascular outcomes.https://doi.org/10.1002/14651858.CD008613.pub2pubpub

Serine one-carbon catabolism with formate overflow

Serine catabolism to glycine and a one-carbon unit has been linked to the anabolic requirements of proliferating mammalian cells. However, genome-scale modeling predicts a catabolic role with one-carbon release as formate. We experimentally prove that in cultured cancer cells and nontransformed fibroblasts, most of the serine-derived one-carbon units are released from cells as formate, and that formate release is dependent on mitochondrial reverse 10-CHO-THF synthetase activity. We also show that in cancer cells, formate release is coupled to mitochondrial complex I activity, whereas in nontransformed fibroblasts, it is partially insensitive to inhibition of complex I activity. We demonstrate that in mice, about 50% of plasma formate is derived from serine and that serine starvation or complex I inhibition reduces formate synthesis in vivo. These observations transform our understanding of one-carbon metabolism and have implications for the treatment of diabetes and cancer with complex I inhibitors

Audit of walk-in access for members of the public to online resources at higher education and further education libraries in the South West of England

This SWRLS-funded project aims to analyse and evaluate the extent to which Higher Education (HE) and Further Education (FE) libraries across the South West region of the United Kingdom provide walk-in access to electronic resources.The information presented within this report is derived from the findings of a survey questionnaire of librarians from across the South West region. Findings of the survey reveal that in the majority of the six institutions that provide walk-in access, the service is not actively promoted. Potential audiences are not actively identified. Information about walk-in services and which resources are available to use within HE or FE in the region is currently hard to discover. There does appear to be some desire to provide walk-in access but the report identifies IT difficulties and legal issues over licences as particular barriers to implementation

A statistical comparison of EUV brightenings observed by SO/EUI with simulated brightenings in nonpotential simulations

Open access funding provided by Swiss Federal Institute of Technology Zurich. L.H. and K.B. are grateful to the SNF for the funding of the project number 200021_188390. D.H.M. would like to thank the STFC for support via consolidated grant ST/W001195/1. K.A.M. would like to thank the STFC for support via consortium grant ST/W001098/1.The High Resolution Imager (HRIEUV) telescope of the Extreme Ultraviolet Imager (EUI) instrument onboard Solar Orbiter has observed EUV brightenings, so-called campfires, as fine-scale structures at coronal temperatures. The goal of this paper is to compare the basic geometrical (size, orientation) and physical (intensity, lifetime) properties of the EUV brightenings with regions of energy dissipation in a nonpotential coronal magnetic-field simulation. In the simulation, HMI line-of-sight magnetograms are used as input to drive the evolution of solar coronal magnetic fields and energy dissipation. We applied an automatic EUV-brightening detection method to EUV images obtained on 30 May 2020 by the HRIEUV telescope. We applied the same detection method to the simulated energy dissipation maps from the nonpotential simulation to detect simulated brightenings. We detected EUV brightenings with a density of 1.41×10−3 brightenings/Mm2 in the EUI observations and simulated brightenings between 2.76×10−2 – 4.14×10−2 brightenings/Mm2 in the simulation, for the same time range. Although significantly more brightenings were produced in the simulations, the results show similar distributions of the key geometrical and physical properties of the observed and simulated brightenings. We conclude that the nonpotential simulation can successfully reproduce statistically the characteristic properties of the EUV brightenings (typically with more than 85% similarity); only the duration of the events is significantly different between observations and simulation. Further investigations based on high-cadence and high-resolution magnetograms from Solar Orbiter are under consideration to improve the agreement between observation and simulation.Publisher PDFPeer reviewe