Grupo de apoio aos pais na prevenção de acidentes infantis no ambulatório de pediatria: um relato de experiência

Introdução: O curso de graduação em enfermagem tem seu objetivo pautado na formação de profissionais capacitados para atuação em diversas áreas desde a assistência, o gerenciamento de serviços de saúde, ensino e pesquisa, tornando fundamental que formação perpasse por diversos campos para experiência. Objetivo: Relatar a experiência acadêmica vivenciada em um projeto de extensão que visou promover um amplo acesso de informações dentro da temática prevenção de acidentes na infância, realizado em um hospital de Ensino Público de alta complexidade no Município de Uberlândia no ano de 2022. Metodologia: Trata-se de um relato de experiência descritivo reflexivo de acadêmicos do curso de enfermagem de uma Universidade Pública no interior de Minas Gerais. O estudo se refere ao componente curricular do projeto de extensão denominado “Grupo de apoio aos pais na prevenção de acidentes infantis no ambulatório de pediatria”, criado e desenvolvido em 2022. A condução da experiência dividiu-se em três etapas, organização de grupo de estudo e de um cronograma de estudos voltados para a temática a ser abordada, produção de folder informativo e organização de roda de conversa direcionada, ampla e acessível aos familiares das crianças atendidas no serviço durante o período da experiência, com a temática central “Acidentes na Infância". Resultados: A experiência se deu em um Hospital Público Universitário e contou com a participação de em média 330 participantes, com 88 pacientes pediátricos aos quais foram expostos ao conteúdo proposto. Observou-se a relevância da educação em saúde e abordagem da temática com a ampla troca de experiências prévias trazidas pelos participantes aos discentes, assim como um momento de treinamento para a inserção do discente no campo de atuação da enfermagem, sendo fundamental para proporcionar reflexão de como atividades de educação são importantes para a prevenção de agravos. Conclusão: Com o desenvolvimento do projeto, foi possível refletir como atividades educacionais são importantes para a formação do enfermeiro baseado em competências para além da técnica, o exercício da capacidade em transpor o conhecimento gerado na Universidade para a população apresentou o potencial de impactar positivamente indicadores de saúde relacionados aos acidentes, vez que grande parte da população não possui acesso a ações de conscientização e prevenção a agravos em saúde

The kSORT assay to detect renal transplant patients at high risk for acute rejection: results of the multicenter AART study

Abstract Background: Development of noninvasive molecular assays to improve disease diagnosis and patient monitoring is a critical need. In renal transplantation, acute rejection (AR) increases the risk for chronic graft injury and failure. Noninvasive diagnostic assays to improve current late and nonspecific diagnosis of rejection are needed. We sought to develop a test using a simple blood gene expression assay to detect patients at high risk for AR. Methods and Findings: We developed a novel correlation-based algorithm by step-wise analysis of gene expression data in 558 blood samples from 436 renal transplant patients collected across eight transplant centers in the US, Mexico, and Spain between 5 February 2005 and 15 December 2012 in the Assessment of Acute Rejection in Renal Transplantation (AART)study. Gene expression was assessed by quantitative real-time PCR (QPCR) in one center. A 17-gene set the Kidney Solid Organ Response Test (kSORT) was selected in 143 samples for AR classification using discriminant analysis (area under the receiver operating characteristic curve [AUC] = 0.94; 95% CI 0.91-0.98), validated in 124 independent samples (AUC = 0.95; 95% CI 0.88-1.0) and evaluated for AR prediction in 191 serial samples, where it predicted AR up to 3 mo prior to detection by the current gold standard (biopsy). A novel reference-based algorithm (using 13 12-gene models) was developed in 100 independent samples to provide a numerical AR risk score, to classify patients as high risk versus low risk for AR. kSORT was able to detect AR in blood independent of age, time post-transplantation, and sample source without additional data normalization; AUC = 0.93 (95% CI 0.86-0.99). Further validation of kSORT is planned in prospective clinical observational and interventional trials. Conclusions: The kSORT blood QPCR assay is a noninvasive tool to detect high risk of AR of renal transplants

Trypanosoma cruzi Gene Expression in Response to Gamma Radiation

Trypanosoma cruzi is an organism highly resistant to ionizing radiation. Following a dose of 500 Gy of gamma radiation, the fragmented genomic DNA is gradually reconstructed and the pattern of chromosomal bands is restored in less than 48 hours. Cell growth arrests after irradiation but, while DNA is completely fragmented, RNA maintains its integrity. In this work we compared the transcriptional profiles of irradiated and non-irradiated epimastigotes at different time points after irradiation using microarray. In total, 273 genes were differentially expressed; from these, 160 were up-regulated and 113 down-regulated. We found that genes with predicted functions are the most prevalent in the down-regulated gene category. Translation and protein metabolic processes, as well as generation of precursor of metabolites and energy pathways were affected. In contrast, the up-regulated category was mainly composed of obsolete sequences (which included some genes of the kinetoplast DNA), genes coding for hypothetical proteins, and Retrotransposon Hot Spot genes. Finally, the tyrosyl-DNA phosphodiesterase 1, a gene involved in double-strand DNA break repair process, was up-regulated. Our study demonstrated the peculiar response to ionizing radiation, raising questions about how this organism changes its gene expression to manage such a harmful stress

Mitochondrial physiology

As the knowledge base and importance of mitochondrial physiology to evolution, health and disease expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow the latest SI guidelines and those of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols with the nomenclature of classical bioenergetics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of data repositories of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery

Mitochondrial physiology

As the knowledge base and importance of mitochondrial physiology to evolution, health and disease expands, the necessity for harmonizing the terminology concerning mitochondrial respiratory states and rates has become increasingly apparent. The chemiosmotic theory establishes the mechanism of energy transformation and coupling in oxidative phosphorylation. The unifying concept of the protonmotive force provides the framework for developing a consistent theoretical foundation of mitochondrial physiology and bioenergetics. We follow the latest SI guidelines and those of the International Union of Pure and Applied Chemistry (IUPAC) on terminology in physical chemistry, extended by considerations of open systems and thermodynamics of irreversible processes. The concept-driven constructive terminology incorporates the meaning of each quantity and aligns concepts and symbols with the nomenclature of classical bioenergetics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in terms of metabolic flows and fluxes. Uniform standards for evaluation of respiratory states and rates will ultimately contribute to reproducibility between laboratories and thus support the development of data repositories of mitochondrial respiratory function in species, tissues, and cells. Clarity of concept and consistency of nomenclature facilitate effective transdisciplinary communication, education, and ultimately further discovery

Influência do número de espermatozóides utilizados na inseminação sobre o transporte espermático e a reação inflamatória do útero de éguas

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Influência do número de espermatozóides utilizados na inseminação sobre o transporte espermático e a reação inflamatória do útero de éguas

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