Molecular electrometer and binding of cations to phospholipid bilayers

Despite the vast amount of experimental and theoretical studies on the binding affinity of cations -especially the biologically relevant Na+ and Ca2+ - for phospholipid bilayers, there is no consensus in the literature. Here we show that by interpreting changes in the choline headgroup order parameters according to the 'molecular electrometer' concept [Seelig et al., Biochemistry, 1987, 26, 7535], one can directly compare the ion binding affinities between simulations and experiments. Our findings strongly support the view that in contrast to Ca2+ and other multivalent ions, Na+ and other monovalent ions (except Li+) do not specifically bind to phosphatidylcholine lipid bilayers at sub-molar concentrations. However, the Na+ binding affinity was overestimated by several molecular dynamics simulation models, resulting in artificially positively charged bilayers and exaggerated structural effects in the lipid headgroups. While qualitatively correct headgroup order parameter response was observed with Ca2+ binding in all the tested models, no model had sufficient quantitative accuracy to interpret the Ca2+: lipid stoichiometry or the induced atomistic resolution structural changes. All scientific contributions to this open collaboration work were made publicly, using nmrlipids. blogspot.fi as the main communication platform.Peer reviewe

Competition on Counter Measures to 2-D Facial Spoofing Attacks

Spoofing identities using photographs is one of the most common techniques to attack 2-D face recognition systems. There seems to exist no comparative studies of different techniques using the same protocols and data. The motivation behind this competition is to compare the performance of different state-of-the-art algorithms on the same database using a unique evaluation method. Six different teams from universities around the world have participated in the contest. Use of one or multiple techniques from motion, texture analysis and liveness detection appears to be the common trend in this competition. Most of the algorithms are able to clearly separate spoof attempts from real accesses. The results suggest the investigation of more complex attacks