Effect of photodynamic therapy and non-thermal plasma on root canal filling: analysis of adhesion and sealer penetration

Objective The aim of this study was to evaluate the effect of photodynamic therapy (PDT) and non-thermal plasma (NTP) on adhesion and sealer penetration in root canals. Material and Methods Sixty single-rooted premolars were used. The teeth were prepared using a crown-down technique. NaOCl and EDTA were used for irrigation and smear layer removal, respectively. The root canals were divided into three groups: control, PDT, and NTP. After treatments, the roots were filled using gutta-percha and either AH Plus (AHP) or MTA Fillapex (MTAF) sealers. Samples were sectioned at 4, 8, and 12 mm from the apex (1-mm slices)and analyzed by the push-out bond strength test (adhesion) and confocal laser scanning microscopy (sealer penetration). Data were statistically evaluated using Kruskal-Wallis, Dunn’s, and Spearman’s tests. Results Regarding AHP, bond strength was similar in the NTP group and in the control group, but significantly lower in the PDT group. As to MTAF, both therapies showed lower values than the control group. In the confocal analysis of AHP, maximum and mean penetration, and penetrated area were statistically higher in the control group than in the PDT and NTP groups. Penetrated perimeter was similar among groups. Regarding MTAF, all parameters yielded better results in the NTP than in the control group. The PDT and control groups showed similar results except for penetrated area. Conclusion PDT and plasma therapy affected the adhesion and sealer penetration of root canals filled with AH Plus and MTA Fillapex and there is no positive correlation between adhesion and sealer penetration

A microleakage study of gutta-percha/AH Plus and Resilon/Real self-etch systems after different irrigation protocols

The development and maintenance of the sealing of the root canal system is the key to the success of root canal treatment. The resin-based adhesive material has the potential to reduce the microleakage of the root canal because of its adhesive properties and penetration into dentinal walls. Moreover, the irrigation protocols may have an influence on the adhesiveness of resin-based sealers to root dentin. Objective: The objective of the present study was to evaluate the effect of different irrigant protocols on coronal bacterial microleakage of gutta-percha/AH Plus and Resilon/Real Seal Self-etch systems. Material and Methods: One hundred ninety pre-molars were used. The teeth were divided into 18 experimental groups according to the irrigation protocols and filling materials used. The protocols used were: distilled water; sodium hypochlorite (NaOCl)+eDTA; NaOCl+H3PO4; NaOCl+eDTA+chlorhexidine (CHX); NaOCl+H3PO4+CHX; CHX+eDTA; CHX+ H3PO4; CHX+eDTA+CHX and CHX+H3PO4+CHX. Gutta-percha/AH Plus or Resilon/Real Seal Se were used as root-filling materials. The coronal microleakage was evaluated for 90 days against Enterococcus faecalis. Data were statistically analyzed using Kaplan-Meier survival test, Kruskal-Wallis and Mann-Whitney tests. Results: No significant difference was verified in the groups using chlorhexidine or sodium hypochlorite during the chemo-mechanical preparation followed by eDTA or phosphoric acid for smear layer removal. The same results were found for filling materials. However, the statistical analyses revealed that a final flush with 2% chlorhexidine reduced significantly the coronal microleakage. Conclusion: A final flush with 2% chlorhexidine after smear layer removal reduces coronal microleakage of teeth filled with gutta-percha/AH Plus or Resilon/Real Seal SE

A Microleakage Study Of Gutta-percha/ah Plus And Resilon/real Self-etch Systems After Different Irrigation Protocols.

The development and maintenance of the sealing of the root canal system is the key to the success of root canal treatment. The resin-based adhesive material has the potential to reduce the microleakage of the root canal because of its adhesive properties and penetration into dentinal walls. Moreover, the irrigation protocols may have an influence on the adhesiveness of resin-based sealers to root dentin. The objective of the present study was to evaluate the effect of different irrigant protocols on coronal bacterial microleakage of gutta-percha/AH Plus and Resilon/Real Seal Self-etch systems. One hundred ninety pre-molars were used. The teeth were divided into 18 experimental groups according to the irrigation protocols and filling materials used. The protocols used were: distilled water; sodium hypochlorite (NaOCl)+eDTA; NaOCl+H3PO4; NaOCl+eDTA+chlorhexidine (CHX); NaOCl+H3PO4+CHX; CHX+eDTA; CHX+ H3PO4; CHX+eDTA+CHX and CHX+H3PO4+CHX. Gutta-percha/AH Plus or Resilon/Real Seal Se were used as root-filling materials. The coronal microleakage was evaluated for 90 days against Enterococcus faecalis. Data were statistically analyzed using Kaplan-Meier survival test, Kruskal-Wallis and Mann-Whitney tests. No significant difference was verified in the groups using chlorhexidine or sodium hypochlorite during the chemo-mechanical preparation followed by eDTA or phosphoric acid for smear layer removal. The same results were found for filling materials. However, the statistical analyses revealed that a final flush with 2% chlorhexidine reduced significantly the coronal microleakage. A final flush with 2% chlorhexidine after smear layer removal reduces coronal microleakage of teeth filled with gutta-percha/AH Plus or Resilon/Real Seal SE.22174-

A microleakage study of gutta-percha/AH Plus and Resilon/Real self-etch systems after different irrigation protocols

The development and maintenance of the sealing of the root canal system is the key to the success of root canal treatment. The resin-based adhesive material has the potential to reduce the microleakage of the root canal because of its adhesive properties and penetration into dentinal walls. Moreover, the irrigation protocols may have an influence on the adhesiveness of resin-based sealers to root dentin. Objective: The objective of the present study was to evaluate the effect of different irrigant protocols on coronal bacterial microleakage of gutta-percha/AH Plus and Resilon/Real Seal Self-Etch systems. Material and Methods: One hundred ninety pre-molars were used. The teeth were divided into 18 experimental groups according to the irrigation protocols and filling materials used. The protocols used were: distilled water; sodium hypochlorite (NaOCl)+EDTA; NaOCl+H3PO4; NaOCl+EDTA+chlorhexidine (CHX); NaOCl+H3PO4+CHX; CHX+EDTA; CHX+ H3PO4; CHX+EDTA+CHX and CHX+H3PO4+CHX. Gutta-percha/AH Plus or Resilon/Real Seal SE were used as root-filling materials. The coronal microleakage was evaluated for 90 days against Enterococcus faecalis. Data were statistically analyzed using Kaplan-Meier survival test, Kruskal-Wallis and Mann-Whitney tests. Results: No significant difference was verified in the groups using chlorhexidine or sodium hypochlorite during the chemo-mechanical preparation followed by EDTA or phosphoric acid for smear layer removal. The same results were found for filling materials. However, the statistical analyses revealed that a final flush with 2% chlorhexidine reduced significantly the coronal microleakage. Conclusion: A final flush with 2% chlorhexidine after smear layer removal reduces coronal microleakage of teeth filled with gutta-percha/AH Plus or Resilon/Real Seal SE223174179CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP302575/2009-0sem informação2009/53976-0; 2010/50817-

The Multidisciplinary Management of Avulsed Teeth: A Case Report

This paper reports multidisciplinary treatment of a dental trauma case to achieve a favorable prognosis. A healthy 14-year-old girl reported avulsion of teeth 11 and 21 which had occurred three months earlier. The initial treatment consisting of replantation with a semi-rigid splint was performed in hospital. At presentation, the patient was still using the semi-rigid splint. The clinical examination revealed the presence of increased mobility in teeth 11 and 21, and absence of vitality in both. Radiographic examination showed the presence of inflammatory external root resorption in both teeth. The treatment proposed consisted of teeth extraction, a temporary prosthesis followed by adhesive prosthesis, and finally, implant surgery associated with porcelain crowns

Comunicação silenciosa mãe-bebê na visão winnicottiana: reflexões teórico-clínicas

Objetivou-se discutir a comunicação silenciosa entre mãe e bebê, a partir do pensamento de Winnicott. Fez-se uma pesquisa qualitativa, baseada no método clínico e referencial psicanalítico, por meio do estudo de caso de uma criança, com 8 anos de idade e dificuldades no desenvolvimento da fala, sem causa orgânica, atendida em psicoterapia de orientação psicodinâmica, em que aspectos teóricos são ilustrados pelo atendimento clínico realizado. No caso relatado, foram levantadas hipóteses de a mãe haver falhado em propiciar ao filho um ambiente facilitador durante os primeiros meses de vida, assim como houve falhas do pai e do ambiente por não terem propiciado o suporte emocional satisfatório para a genitora. A dificuldade de expressão verbal da criança estava relacionada com a manutenção de uma comunicação silenciosa primitiva, que significaria a garantia da sobrevivência psíquica, diante de um ambiente que não havia se configurado, até então, como suficientemente bom

Sistema de armazenamento e incidência dos principais fungos produtores de micotoxinas em grãos / Storage system and incidence of the main fungi that produce mycotoxins in grains

O déficit de armazenamento e um grave problema enfrentado no Brasil, o que causou investimentos em tecnologias no campo e em unidades armazenadores. O objetivo desta revisão literária é identificar à ocorrência de possíveis contaminações por fungos toxigênicos produtores de micotoxinas em grãos armazenados em silos hermeticamente fechados (silo bolsa). O referencial literário trás conceitos sobre os diferentes sistemas de armazenamento de grãos, suas vantagens, desvantagens e principais fungos produtores de micotoxinas. Os grãos são produtos muito suscetível à contaminação por fungos, constantemente facilitada pelas condições de armazenamento. A umidade e temperatura elevadas no interior das unidades de armazenamento, associadas a deficiências no manejo operacional, potencializam esses efeitos. Construiu-se um referencial teórico que contempla o conceito central e o real potencial dos silos hermeticamente fechados, como novo modelo de armazenamento utilizado pelos produtores, que buscam diminuir os custos e danos, visando à conservação dos grãos. O uso dessa tecnologia traz várias vantagens para o produtor, porém as diferenças climáticas do País e o fato do silo bolsa estar diretamente no campo, sujeito a todo tipo de ataque e condições climáticas adversas, causa dúvidas quanto à eficiência do armazenamento

Preparo de canais de molares inferiores com diferentes anatomias: estudo por microtomografia computadorizada: Root canal preparation of mandibular molars with different anatomies: a microcomputed tomography study

Objetivo: avaliar, in vitro, o preparo de molares inferiores com diferentes anatomias (tipo II e IV de Vertucci), empregando o sistema TruNatomy no que diz respeito à área de superfície, volume e área não preparada. Matérias e Métodos: 30 molares inferiores foram escaneados em um microtomógrafo e divididos em dois grupos de acordo com a anatomia dos canais presentes na raiz mesial (n=8): Configuração tipo II de Vertucci (Dois canais deixam a câmara pulpar e se juntam próximo ao ápice para formar um único canal) e Configuração tipo IV de Vertucci (Dois canais separados e distintos se estendem da câmara pulpar até́ o ápice). Os dentes foram acessados e a patência determinada até a saída foraminal. O comprimento de trabalho (CT) foi estabelecido em 1mm aquém da patência. O preparo dos canais foi realizado com o sistema TruNatomy de acordo com as recomendações do fabricante. Terminado o preparo, os dentes foram novamente escaneados e analisados quanto à variação de volume, área e a porcentagem de região não preparada. Os dados foram avaliados estatisticamente pelos testes de Mann-Whitney e Wilcoxon (p < 0,05). Resultados: Houve um aumento na área e no volume dos canais radiculares após o preparo ( p < 0,05) , independente da anatomia dos canais. Não houve diferença significativa em relação a porcentagem de área não preparada, área de superfície e volume dos canais quando as diferentes anatomias foram comparadas (p > 0,05). Conclusão: As variações anatômicas dos molares inferiores não influenciaram no volume, na área e na porcentagem de áreas não preparada ao utilizar o sistema TruNatomy

Antimicrobial and cytotoxic effects of phosphoric acid solution compared to other root canal irrigants

Phosphoric acid has been suggested as an irrigant due to its effectiveness in removing the smear layer. The purpose of this study was to compare the antimicrobial and cytotoxic effects of a 37% phosphoric acid solution to other irrigants commonly used in endodontics. The substances 37% phosphoric acid, 17% EDTA, 10% citric acid, 2% chlorhexidine (solution and gel), and 5.25% NaOCl were evaluated. The antimicrobial activity was tested against Candida albicans, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Actinomyces meyeri, Parvimonas micra, Porphyromonas gingivalis, and Prevotella nigrescensaccording to the agar diffusion method. The cytotoxicity of the irrigants was determined by using the MTT assay. Phosphoric acid presented higher antimicrobial activity compared to the other tested irrigants. With regard to the cell viability, this solution showed results similar to those with 5.25% NaOCl and 2% chlorhexidine (gel and solution), whereas 17% EDTA and 10% citric acid showed higher cell viability compared to other irrigants. Phosphoric acid demonstrated higher antimicrobial activity and cytotoxicity similar to that of 5.25% NaOCl and 2% chlorhexidine (gel and solution).232158163CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO - CNPQCOORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIOR - CAPESFUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP302575/2009-0Sem informação2009/53976-0; 2010/50817-5; 2011/09047-

Importância dos remineralizadores na produção de milho para silagem / Importance of remineralizers in the production of corn for silage

Produtores rurais têm aderido alternativas de agriculturas econômicas e sustentáveis de tecnologias aliadas aos fertilizantes convencionais. A remineralização do solo na agricultura tem priorizado a prática do uso de pó de rocha, que permite em condições de solos empobrecidos nutricionalmente a viabilidade de potencializar o efeito nutricional da planta. O cultivo de milho por expansão se consagra como maior cultura agrícola do mundo, bem como a agropecuária exige por grãos de melhor nutrição e produção, e por proporcionar a qualidade na dieta do animal. Em época de escassez de pastagens uma prática agrícola em uso é a ensilagem, método de preservação de qualidade da forragem por processo de armazenamento, sendoo milho o mais utilizado por apresentar características ideais em condições de rendimento de matéria seca e bom perfil fermentativo. Objetiva-se avaliar a silagem de milho produzido em solo remineralizado. O referencial literário conceitua remineralizadores, fatores que afetam a qualidade da silagem, caracterização da ensilagem de milho e características do milho para ensilagem. Apresentando o pó de rocha na remineralização de solos em diferentes culturas com resultados positivos, porém o milho produzido em solo remineralizados para a produção de silagem, percebe-se a escassez de pesquisas, necessitando de pesquisas futuras