A study investigating the comparative situation awareness of older and younger drivers when driving a route with extended periods of cognitive taxation

This study sought to measure and compare the Situation Awareness (SA) of a younger group of 11 drivers (average age 28.2 years) to that of an older group of 10 drivers (average age 77.2 years), as they traversed a route that included many cognitively taxing elements. This was achieved by recording a participant’s continual commentary of what s/he felt to be of relevance during the drive. These recordings were then transcribed and assessed by computer software capable of abstracting the main concepts from each individual’s or group’s narrative, and calculating scores indicative of Situation Awareness. It was found that the younger drivers scored significantly higher (p < 0.024) than their older counterparts. Furthermore, when the results from the participants who undertook both this and previous studies in the series were compared (see Key, Morris, & Mansfield, 2016), it was found that SA scoring could be importantly influenced by perceptions of a task’s difficulty, rather than its actual difficulty. It was also indicative from the narratives, that the younger driving group had demonstrated a better 360-degree awareness, and enunciated more safety-related concepts

Situation Awareness: its proficiency amongst older and younger drivers, and its usefulness for perceiving hazards.

The two studies reported here sought to measure and compare the Situation Awareness (SA) of younger and older driver groups whilst driving (Study 1), and watching video footage of actual car journeys (Study 2). In both studies this was achieved by recording a participant’s commentary on what s/he felt was of relevance to the driving task. The narratives produced were analysed by computer software that could abstract main concepts and calculate scores indicative of Situation Awareness. In Study 2, these scores were related to others for hazard perception proficiency (also derived from participant commentaries). It was found that the older drivers matched and often exceeded the younger drivers when their SA scores were compared individually, but not when assessed as a group. However, the younger drivers out-performed their older counterparts in hazard perception ability, and this was shown to be related to their Situation Awareness score. When the results from participants who undertook both studies were compared, it was found that Situation Awareness performance was significantly higher when commenting on video footage (Study 2) than whilst actually driving (Study 1)

Novel phages of Pseudomonas syringae unveil numerous potential auxiliary metabolic genes.

Relatively few phages that infect plant pathogens have been isolated and investigated. The Pseudomonas syringae species complex is present in various environments, including plants. It can cause major crop diseases, such as bacterial canker on apricot trees. This study presents a collection of 25 unique phage genomes that infect P. syringae. These phages were isolated from apricot orchards with bacterial canker symptoms after enrichment with 21 strains of P. syringae. This collection comprises mostly virulent phages, with only three being temperate. They belong to 14 genera, 11 of which are newly discovered, and 18 new species, revealing great genetic diversity within this collection. Novel DNA packaging systems have been identified bioinformatically in one of the new phage species, but experimental confirmation is required to define the precise mechanism. Additionally, many phage genomes contain numerous potential auxiliary metabolic genes with diversified putative functions. At least three phages encode genes involved in bacterial tellurite resistance, a toxic metalloid. This suggests that viruses could play a role in bacterial stress tolerance. This research emphasizes the significance of continuing the search for new phages in the agricultural ecosystem to unravel novel ecological diversity and new gene functions. This work contributes to the foundation for future fundamental and applied research on phages infecting phytopathogenic bacteria

Resource Recovery from Wastewater Treatment Sludge Containing Gypsum

The disposal of wastewater treatment sludge generated at the Radford Army Ammunition Plant (RAAP) is a serious problem. The sludge is produced by neutralizing spent acid contained in the wastewater with lime, and consists principally of very finely divided wet gypsum (calcium sulfate dihydrate). Although the sludge is presently being disposed of in a landfill, the sludge is difficult to handle and convert into a load-bearing material. Therefore an alternative method of disposal is being developed and evaluated. The alternative method involves drying and granulating the sludge, followed by high temperature calcination in a fluidized bed reactor to recover usable sulfur dioxide and lime. If the method is adopted, these products would be used within the plant with considerable cost savings. The sulfur dioxide would be added to the feed stream of an oleum manufacturing facility and the lime would be reused in wastewater treatment

Survival and ice nucleation activity of bacteria as aerosols in a cloud simulation chamber

The residence time of bacterial cells in the atmosphere is predictable by numerical models. However, estimations of their aerial dispersion as living entities are limited by a lack of information concerning survival rates and behavior in relation to atmospheric water. Here we investigate the viability and ice nucleation (IN) activity of typical atmospheric ice nucleation active bacteria (Pseudomonas syringae and P. fluorescens) when airborne in a cloud simulation chamber (AIDA, Karlsruhe, Germany). Cell suspensions were sprayed into the chamber and aerosol samples were collected by impingement at designated times over a total duration of up to 18 h, and at some occasions after dissipation of a cloud formed by depressurization. Aerosol concentration was monitored simultaneously by online instruments. The cultivability of airborne cells decreased exponentially over time with a half-life time of 250 ±30 min (about 3.5 to 4.5 h). In contrast, IN activity remained unchanged for several hours after aerosolization, demonstrating that IN activity was maintained after cell death. Interestingly, the relative abundance of IN active cells still airborne in the chamber was strongly decreased after cloud formation and dissipation. This illustrates the preferential precipitation of IN active cells by wet processes. Our results indicate that from 10⁶ cells aerosolized from a surface, one would survive the average duration of its atmospheric journey estimated at 3.4 days. Statistically, this corresponds to the emission of 1 cell that achieves dissemination every ~33 min m¯² of cultivated crops fields, a strong source of airborne bacteria. Based on the observed survival rates, depending on wind speed, the trajectory endpoint could be situated several hundreds to thousands of kilometers from the emission source. These results should improve the representation of the aerial dissemination of bacteria in numeric models

Human airway smooth muscle maintain in situ cell orientation and phenotype when cultured on aligned electrospun scaffolds

Human airway smooth muscle (HASM) contraction plays a central role in regulating airway resistance in both healthy and asthmatic bronchioles. In vitro studies that investigate the intricate mechanisms that regulate this contractile process are predominantly conducted on tissue culture plastic, a rigid, 2D geometry, unlike the 3D microenvironment smooth muscle cells are exposed to in situ. It is increasingly apparent that cellular characteristics and responses are altered between cells cultured on 2D substrates compared with 3D topographies. Electrospinning is an attractive method to produce 3D topographies for cell culturing as the fibers produced have dimensions within the nanometer range, similar to cells' natural environment. We have developed an electrospun scaffold using the nondegradable, nontoxic, polymer polyethylene terephthalate (PET) composed of uniaxially orientated nanofibers and have evaluated this topography's effect on HASM cell adhesion, alignment, and morphology. The fibers orientation provided contact guidance enabling the formation of fully aligned sheets of smooth muscle. Moreover, smooth muscle cells cultured on the scaffold present an elongated cell phenotype with altered contractile protein levels and distribution. HASM cells cultured on this scaffold responded to the bronchoconstrictor bradykinin. The platform presented provides a novel in vitro model that promotes airway smooth muscle cell development toward a more in vivo-like phenotype while providing topological cues to ensure full cell alignment

Kinetic energy extraction of a tidal stream turbine and its sensitivity to structural stiffness attenuation

© 2015 The Authors. The hydrodynamic forces imparted on a tidal turbine rotor, whilst causing it to rotate and hence generate power, will also cause the blades to deform. This deformation will affect the turbine's performance if not included in the early design phase and could lead to a decrease in power output and a reduction in operational life. Conversely, designing blades to allow them to deform slightly may reduce localised stress and therefore prolong the life of the blades and allow the blades to deform in to their optimum operational state. The aim of this paper is to better understand the kinetic energy extraction by varying the material modulus of a turbine blade. Shaft torque/power, blade tip displacement, and axial thrust results are presented for 2, 3 and 4 bladed rotor configurations at peak power extraction. For the rotor design studied the FSI model data show that there is a low sensitivity to blade deformation for the 2, 3 and 4 bladed rotors. However, the results reveal that the 3 bladed rotor displayed maximum hydrodynamic performance as a rigid structure which then decreased as the blade deformed. The 2 and 4 bladed rotor configurations elucidated a slight increase in hydrodynamic performance with deflection

The effect of tidal flow directionality on tidal turbine performance characteristics

With many Tidal Energy Conversion (TEC) devices at full scale prototype stage there are two distinct design groups for Horizontal Axis Tidal Turbines (HATTs). Devices with a yaw mechanism allowing the turbine to always face into the flow, and devices with blades that can rotate through 180° to harness a strongly bi-directional flow. As marine turbine technology verges on the realm of economic viability this paper reveals the performance of Cardiff University's concept tidal turbine with its support structure either upstream or downstream and with various proximities between the rotating plane of the turbine and its support stanchion. Through the use of validated Computational Fluid Dynamics (CFD) modelling this work shows the optimal proximity between rotor plane and stanchion as well as establishing, in the given context, the use of a yaw mechanism to be superior to a bi-directional system from a performance perspective

Interactions between proteins bound to biomembranes

We study a physical model for the interaction between general inclusions bound to fluid membranes that possess finite tension, as well as the usual bending rigidity. We are motivated by an interest in proteins bound to cell membranes that apply forces to these membranes, due to either entropic or direct chemical interactions. We find an exact analytic solution for the repulsive interaction between two similar circularly symmetric inclusions. This repulsion extends over length scales of order tens of nanometers, and contrasts with the membrane-mediated contact attraction for similar inclusions on tensionless membranes. For non circularly symmetric inclusions we study the small, algebraically long-ranged, attractive contribution to the force that arises. We discuss the relevance of our results to biological phenomena, such as the budding of caveolae from cell membranes and the striations that are observed on their coats.Comment: 22 pages, 2 figure

Elastic deformation of a fluid membrane upon colloid binding

When a colloidal particle adheres to a fluid membrane, it induces elastic deformations in the membrane which oppose its own binding. The structural and energetic aspects of this balance are theoretically studied within the framework of a Helfrich Hamiltonian. Based on the full nonlinear shape equations for the membrane profile, a line of continuous binding transitions and a second line of discontinuous envelopment transitions are found, which meet at an unusual triple point. The regime of low tension is studied analytically using a small gradient expansion, while in the limit of large tension scaling arguments are derived which quantify the asymptotic behavior of phase boundary, degree of wrapping, and energy barrier. The maturation of animal viruses by budding is discussed as a biological example of such colloid-membrane interaction events.Comment: 14 pages, 9 figures, REVTeX style, follow-up on cond-mat/021242