Peroxyoxalate chemiluminescence detection for the highly sensitive determination of fluorescence-labeled chlorpheniramine with Suzuki coupling reaction.

A sensitive and selective high performance liquid chromatography-peroxyoxalate chemiluminescence (PO-CL) method has been developed for the simultaneous determination of chlorpheniramine (CPA) and monodesmethyl chlorpheniramine (MDCPA) in human serum. The method combines fluorescent labeling with 4-(4,5-diphenyl-1H-imidazole-2-yl)phenyl boronic acid using Suzuki coupling reaction with PO-CL detection. CPA and MDCPA were extracted from human serum by liquid-liquid extraction with n-hexane. Excess labeling reagent, which interfered with trace level determination of analytes, was removed by solid-phase extraction using a C18 cartridge. Separation of derivatives of both analytes was achieved isocratically on a silica column with a mixture of acetonitrile and 60 mM imidazole-HNO(3) buffer (pH 7.2; 85:15, v/v) containing 0.015% triethylamine. The proposed method exhibited a good linearity with a correlation coefficient of 0.999 for CPA and MDCPA within the concentration range of 0.5-100 ng/mL. The limits of detection (S/N = 3) were 0.14 and 0.16 ng/mL for CPA and MDCPA, respectively. Using the proposed method, CPA could be selectively determined in human serum after oral administration

Musical evolution in the lab exhibits rhythmic universals

Music exhibits some cross-cultural similarities, despite its variety across the world. Evidence from a broad range of human cultures suggests the existence of musical universals1, here defined as strong regularities emerging across cultures above chance. In particular, humans demonstrate a general proclivity for rhythm2, although little is known about why music is particularly rhythmic and why the same structural regularities are present in rhythms around the world. We empirically investigate the mechanisms underlying musical universals for rhythm, showing how music can evolve culturally from randomness. Human participants were asked to imitate sets of randomly generated drumming sequences and their imitation attempts became the training set for the next participants in independent transmission chains. By perceiving and imitating drumming sequences from each other, participants turned initially random sequences into rhythmically structured patterns. Drumming patterns developed into rhythms that are more structured, easier to learn, distinctive for each experimental cultural tradition and characterized by all six statistical universals found among world music1; the patterns appear to be adapted to human learning, memory and cognition. We conclude that musical rhythm partially arises from the influence of human cognitive and biological biases on the process of cultural evolution

Enfermagem e Sindicalismo em Goiás: análise do período 1982-2004 Enfermería y Sindicalismo en Goiás: análisis del período 1982-2004 Nursing and Unionism in Goiás: analysis of the period from 1982 to 2004

Estudos sobre Sindicalismo e Enfermagem são bastante escassos no Brasil. Em Goiás, só encontramos uma monografia acerca do tema, o que nos levou a examinar a questão. Este trabalho tem como objetivo analisar o sindicalismo na área de enfermagem em Goiás, buscando reconstituir sua trajetória e evidenciar a ocorrência de articulações com outros sindicatos. A metodologia empregada (qualitativa) embasa-se na História Nova, com o emprego de fontes primárias (entrevistas e documentos originais) e secundárias. As respostas obtidas demonstraram que alguns sindicatos são mais atuantes que outros e que as diferentes possibilidades de afiliação contribuem para enfraquecer o Sindicato dos Enfermeiros de Goiás.<br>Estúdios con respecto al Sindicalismo y Enfermería son bastante raros en el Brasil. En Goiás, sólo encontramos una monografia acerca del tema, lo que nos llevou a examinar esta cuestión. Este trabajo tiene como objetivo analizar el sindicalismo en la área de la enfermería en Goiás, procurando reconstruir su trayectoria y evidenciar la ocurrencia de las articulaciones con otros sindicatos. La metodología utilzada basease en la Historia Nueva, con lo empleo de fuentes primarias y secundarias. Las respuestas obtenidas demonstráron que algunos sindicatos son más actuantes que otros y que las diferentes posibilidades de filiación contribuyen para debilitar el Sindicato de los Enfermeros de Goiás.<br>Studies about Unionism and Nursing are rare in Brazil. In Goiás, we just find out a monograph about the subject, which made us to analyze the question. The objective of this work is to analyze the Unionism of Nursing in Goiás, in search of rebuilt their trajectory and evidence the articulation with others syndicates. The methodology used called "qualitative" based on New History, using primary sources (interview and originals documents) and secondary. The answers we have got showed that some syndicates are much skilled than others and the different possibilities of membership helps to weaken the Union of Nurses in Goiás