Пряма регенерація мікропагонів з листкових дисків ківі (Actinidia delisiosa (Chev.) Liang, Ferguson) в умовах in vitro

The method of direct regeneration of microshoots from leaf disks of kiwi fruit is developed. The factors influencing morphogenetic potency of leaf disks of 4 kiwi fruit cultivars (Bruno, Monti, Tomuri, Sanishton) in condition in vitro are determined. The genetically uniform plant material is obtained.Розроблено спосіб прямої регенерації мікропагонів з листкових дисків ківі. Визначено фактори, що впливають на морфогенетичні потенції листкових дисків 4 сортів ківі (Бруно, Монті, Томурі, Саніштон) в умовах in vitro. Отримано генетично однорідний рослинний матеріал

TENDENCIES, PROBLEMS AND PROSPECTS OF THE BALANCED AND COMPETITIVE DEVELOPMENT OF AGRARIAN ENTREPRENEURSHIP IN RUSSIA

The article displays basic problems and it studies prospects of the development ofthe agrarian entrepreneurship in contemporary Russia. Under the conditions of theforeign economic sanctions, respective Russian counter sanctions and the Russiandirection of a large scale import substitution it is necessary to start the activity in the fieldof the creation of the conditions for the modernization of agrarian technologies ofproduction, in the creation of highly qualified information and analytical services, in thetraining the personnel for the farming, in the increase of the arable surface for workingentrepreneurs doing business in the agricultural sphere. Such measures like the increaseand qualitative improvement of public grasslands and hayfields for cattle from privatesubsidiary farms, development of agricultural cooperation in gathering, storage,processing, formation of lots of goods and the selling of the production of farms andprivate subsidiary farms, development of the infrastructure and logistic provision of ruralareas will allow creating conditions for a competitive and balanced development of theagricultural business

Evaluation of the properties of complexing agent-collectors of eudialyte flotation

Acid-based and complexing properties of reagent-collectors (carboxylic and hydroxamic acids, styrylphosphonic acid and dialkyl ether of phosphoric acid) for flotation of zirconium-containing minerals have been studied. Stability constants for zirconium compounds of investigated reagents have been determined. Eudialyte-containing ore flotation has proved high efficiency of hydroxamic acid

SPECIALLY PROTECTED NATURAL AREAS OF REGIONS OF THE SOUTHERN FEDERAL DISTRICT OF RUSSIA: SPECIFICITY OF APPROACHES TO MANAGEMENT AND CONSERVATION

It is indispensible to pay a particular attention to the regional aspect of creation,functioning and management of the specially protected natural areas at the study of thenatural resource use in the economic districts of Russia and some Russian regions. Theshare of the net of the specially protected natural areas is 11,7% of the surface of theRussian Federation. Under the conditions of a new geoeconomic zonation they create theirecological frame, play an important role in the solution of the problems of theconservation of the biological diversity at the process of the accelerating the neoindustrialization of the economic life of regions. In these territories the maintenance of thestability of the natural area is provided with the help of special natural use and territoryprotection regimes. In the article it is shown which types of the specially protected naturalareas exist in the Southern Federal District of Russia, their specificity, the specificmanagerial approaches are analyzed and variants of their modernization are offered

Specially protected natural areas of regions of the southern federal district of Russia: Specificity of approaches to management and conservation

It is indispensible to pay a particular attention to the regional aspect of creation,functioning and management of the specially protected natural areas at the study of thenatural resource use in the economic districts of Russia and some Russian regions. Theshare of the net of the specially protected natural areas is 11,7% of the surface of theRussian Federation. Under the conditions of a new geoeconomic zonation they create theirecological frame, play an important role in the solution of the problems of theconservation of the biological diversity at the process of the accelerating the neoindustrialization of the economic life of regions. In these territories the maintenance of thestability of the natural area is provided with the help of special natural use and territoryprotection regimes. In the article it is shown which types of the specially protected naturalareas exist in the Southern Federal District of Russia, their specificity, the specificmanagerial approaches are analyzed and variants of their modernization are offered

Tendencies, problems and prospects of the balanced and competitive development of agrarian entrepreneurship in Russia

The article displays basic problems and it studies prospects of the development of the agrarian entrepreneurship in contemporary Russia. Under the conditions of the foreign economic sanctions, respective Russian counter sanctions and the Russian direction of a large scale import substitution it is necessary to start the activity in the field of the creation of the conditions for the modernization of agrarian technologies of production, in the creation of highly qualified information and analytical services, in the training the personnel for the farming, in the increase of the arable surface for working entrepreneurs doing business in the agricultural sphere. Such measures like the increase and qualitative improvement of public grasslands and hayfields for cattle from private subsidiary farms, development of agricultural cooperation in gathering, storage, processing, formation of lots of goods and the selling of the production of farms and private subsidiary farms, development of the infrastructure and logistic provision of rural areas will allow creating conditions for a competitive and balanced development of the agricultural business

Development of novel monoclonal antibodies for evaluation of transmembrane prostate androgen-induced protein 1 (TMEPAI) expression patterns in gastric cancer.

Transmembrane prostate androgen-induced protein 1 (TMEPAI) is a single-span membrane protein, functionally involved in transforming growth factor beta signaling pathway. The particular protein presented in cells in three isoforms, which differs in the length of the soluble N-terminal extracellular domain, making it challenging for the immunochemical recognition. By using quantitative real-time polymerase chain reaction, we identified significant upregulation of PMEPA1 gene expression in malignant tissues of patients with gastric adenocarcinoma. The main part of commercially available anti-TMEPAI antibodies are having polyclonal nature or not suitable for immunocytochemical localization of target protein in tissue specimens. Hence, we decide to generate a set of novel rat monoclonal antibodies (mAb) directed against conservative C-terminal cytoplasmic epitope. Immunoblotting analysis showed that monoclonal antibodies, 2E1, 6C6, and 10A7 were able to recognize specifically target protein in transiently transfected HEK293T and CHO-K1 cells. Especially established mAb, named 10A7, showed the excellent binding ability to target protein in immunohistochemistry. By using developed antibodies, we observed pronounced expression of TMEPAI in normal gastric epithelial cells while tumor cells from gastric adenomas, and adenocarcinoma samples were mostly negative for target protein expression. Also, we found that gastric epithelium cells lose the TMEPAI expression concurrently with severe dysplasia progression, which probably caused by a mechanism involving specific microRNA

Macropropagation and micropropagation of Ziziphus spina-christi Macropropagação e micropropagação de Ziziphus spina-christi

Christ's thorn (Ziziphus spina-christi (L.) Desf.) is a cross-pollinated plant with a wide range of genetic variability in nature and, for this reason, vegetative propagation assumes importance for improvement programs. The objective of this work was to evaluate cutting, T budding and tissue culture methods for this species. Shoots of 22-25 cm length were treated by two culture media and three shoot diameters for cutting trial. The T budding treatments consisted of three and five collection dates in spring and autumn, respectively. Tissue culture nodal segments bearing axillary buds were removed from shoots of mature trees at different seasons. Experiments to determine the best disinfectant chemical, appropriate conditions and materials to prevent phenolic compound exudation, explant characteristics, media type and cytokinin-auxin ratios were carried out. Successful rooting happened only on the sand beds and with cuttings greater than 8 mm diameter. The effects of T budding seasons on budtake percentage were significantly different. The best time for explant harvesting was mid of summer. Amount of rooting on media containing IBA as well as activated charcoal and disinfection with Ca(OCl)2 at concentration of 5% for 20 minutes were the best treatments.<br>Ziziphus spina-christi (L.) Desf. é uma planta de polinização cruzada com grande variabilidade genética na natureza e, por isso, sua propagação vegetativa assume importância para o melhoramento genético. O objetivo deste trabalho foi avaliar os métodos de estaquia, borbulhia em T e cultura de tecido na propagação vegetativa desta espécie. No ensaio de estaquia, partes aéreas da planta com 22-25 cm foram cultivadas em dois meios de cultura e com três diâmetros. O ensaio de borbulhia consistiu na coleta do material em três datas na primavera e em cinco datas no outono. Os segmentos nodais contendo gemas axilares foram removidos da parte aérea de árvores maduras, em diferentes estações do ano, e avaliados em relação à esterilização química, exudação de compostos fenólicos e cultivo. O enraizamento ocorreu apenas em camas de areia e em estacas maiores do que 8 mm. Houve efeito significativo da estação do ano sobre a porcentagem de brotação. O melhor período para coleta do explante foi no verão. O meio contendo IBA e carvão ativado apresentou maior quantidade de raízes e o melhor desinfestante foi Ca(OCl)2 na concentração de 5%, por 20 minutos