Affinity modulation of platelet integrin alphaIIbbeta3 by beta3-endonexin, a selective binding partner of the beta3 integrin cytoplasmic tail.

Platelet agonists increase the affinity state of integrin alphaIIbbeta3, a prerequisite for fibrinogen binding and platelet aggregation. This process may be triggered by a regulatory molecule(s) that binds to the integrin cytoplasmic tails, causing a structural change in the receptor. beta3-Endonexin is a novel 111-amino acid protein that binds selectively to the beta3 tail. Since beta3-endonexin is present in platelets, we asked whether it can affect alphaIIbbeta3 function. When beta3-endonexin was fused to green fluorescent protein (GFP) and transfected into CHO cells, it was found in both the cytoplasm and the nucleus and could be detected on Western blots of cell lysates. PAC1, a fibrinogen-mimetic mAb, was used to monitor alphaIIbbeta3 affinity state in transfected cells by flow cytometry. Cells transfected with GFP and alphaIIbbeta3 bound little or no PAC1. However, those transfected with GFP/beta3-endonexin and alphaIIbbeta3 bound PAC1 specifically in an energy-dependent fashion, and they underwent fibrinogen-dependent aggregation. GFP/beta3-endonexin did not affect levels of surface expression of alphaIIbbeta3 nor did it modulate the affinity of an alphaIIbbeta3 mutant that is defective in binding to beta3-endonexin. Affinity modulation of alphaIIbbeta3 by GFP/beta3-endonexin was inhibited by coexpression of either a monomeric beta3 cytoplasmic tail chimera or an activated form of H-Ras. These results demonstrate that beta3-endonexin can modulate the affinity state of alphaIIbbeta3 in a manner that is structurally specific and subject to metabolic regulation. By analogy, the adhesive function of platelets may be regulated by such protein-protein interactions at the level of the cytoplasmic tails of alphaIIbbeta3

Tinjauan Hukum Islam Terhadap Praktek Akad Pembiayaan Ijarah (Studi Kasus di BMT Al-Madinah Jajar Laweyan Surakarta)

Praktek Ijārah yaitu penjualan manfaat atau salah satu bentuk aktivitas antara dua belah pihak yang berakad guna meringankan salah satu pihak atau saling meringankan, serta termasuk salah satubentuk tolong menolong yang diperintahkan oleh agama. Dalam fiqih islam Ijārah memeberikan sesuatu untuk disewakan, menurut fatwa DSN ijarah didefinisikan sebagai akad pemindahan hak guna(manfaat) atas suatu barang dalam waktu tertentu dengan sewa, tanpa diikuti dengan pemindahan barang itu sendiri. Di dalam penetapan ujroh yang ada di BMT Al-Madinah Jajar Laweyan Surakarta dimana dalam pambagiannya tersebut dengan menggunakan hasil kesepakatan oleh kedua belah pihak yang ada di BMT tesebut, sehingga menjadi peluang peneliti untuk obyek kajian penelitian yang dapat memberikan kontribusi pemikiran dalam bidang Ijārah, khususnya bagi para pelaku praktek yang menggunakan Akad Ijarah. Tujuan dari penelitian ini adalah sebagai berikut: (1) Mengetahui praktek akad pembiayaan Ijārah dalam pembagian ujroh yang ada di BMT Al-Madinah jajar laweyan surakarta. (2) Mengetahui tinjauan hukum Islam terhadap praktek akad pembiayaan ijarah. Penelitaian ini adalah penelitian lapangan (field research ) dengan pendektan kualitatif. Metode yang digunakan dalam pengumpulan data adalah dengan cara wawancara dan dokumentasi. Analisis yang digunakan adalah metode Deduktif dan metode Induktif. Berdasarkan penelitian akad Ijarah yang ada di BMT Al-Madinah Jajar Laweyan Surakarta, pelaksanaan praktek akad pembiayaan Ijārah terdapat dua hukum pertama pada pembiayaan pendidikan dan pembiayaan rumah sakit belum memenuhi syarat berdasarkan konsep ijārah, karna kejelasan barang yang ditransaksikan bukan barang atau jasa akan tetapi berbentuk uang. Kedua pada pembiayaan gedung pengantin sudah sesuai dengan syariah, rukun dan syaratnya sudah terpenuhi meskipun ada pihak penyedia jasa akan tetapi tidak mengurangi dalam kecacatan hukum. Kata Kunci: Ijārah, Akad, Hukum Islam

Anxiety Level of Pregnant Women from Ahwaz Exposed to Domestic Violence

Background: Domestic violence is a concern in the communities and need a wide attention. Violence against women can cause all kinds of physical and mental illness. This is a threat to woman’s and family member’s health. Stress, anxiety and depression during pregnancy are risk factors for adverse consequences. Little is known about the level of the mental illnesses in women who exposed to domestic violence during pregnancy. This study reported the level of anxiety in women who were victims of domestic violence in health centers in Ahwaz.Methods: This was a cross-sectional study in which a sample of 110 pregnant women with domestic violence and pregnant women without violence were recruited.  The data were collected using the demographic questionnaire, World Health Organization standardized domestic violence questionnaire, and the Beck Anxiety Inventory (BAI). The data entered in SPSS 21 and were analyzed using Chi-Square, Independent-t and Multiple Logistic Regression.Results: The pregnant women in the domestic violence group had a higher mean score of anxiety (P<0.05). Women exposed to violence experienced psychological violence (57.75%), physical violence (37.73%) and sexual violence (33.88%). The psychological violence was the greatest influence on the level of anxiety in pregnant women.Conclusion: This study demonstrated the impact of domestic violence on the level of anxiety in pregnant women and confirmed its treat to women's mental health

MultiMetEval: comparative and multi-objective analysis of genome-scale metabolic models

Comparative metabolic modelling is emerging as a novel field, supported by the development of reliable and standardized approaches for constructing genome-scale metabolic models in high throughput. New software solutions are needed to allow efficient comparative analysis of multiple models in the context of multiple cellular objectives. Here, we present the user-friendly software framework Multi-Metabolic Evaluator (MultiMetEval), built upon SurreyFBA, which allows the user to compose collections of metabolic models that together can be subjected to flux balance analysis. Additionally, MultiMetEval implements functionalities for multi-objective analysis by calculating the Pareto front between two cellular objectives. Using a previously generated dataset of 38 actinobacterial genome-scale metabolic models, we show how these approaches can lead to exciting novel insights. Firstly, after incorporating several pathways for the biosynthesis of natural products into each of these models, comparative flux balance analysis predicted that species like Streptomyces that harbour the highest diversity of secondary metabolite biosynthetic gene clusters in their genomes do not necessarily have the metabolic network topology most suitable for compound overproduction. Secondly, multi-objective analysis of biomass production and natural product biosynthesis in these actinobacteria shows that the well-studied occurrence of discrete metabolic switches during the change of cellular objectives is inherent to their metabolic network architecture. Comparative and multi-objective modelling can lead to insights that could not be obtained by normal flux balance analyses. MultiMetEval provides a powerful platform that makes these analyses straightforward for biologists. Sources and binaries of MultiMetEval are freely available from https://github.com/PiotrZakrzewski/MetEv​al/downloads

If players are sparse social dilemmas are too: Importance of percolation for evolution of cooperation

Spatial reciprocity is a well known tour de force of cooperation promotion. A thorough understanding of the effects of different population densities is therefore crucial. Here we study the evolution of cooperation in social dilemmas on different interaction graphs with a certain fraction of vacant nodes. We find that sparsity may favor the resolution of social dilemmas, especially if the population density is close to the percolation threshold of the underlying graph. Regardless of the type of the governing social dilemma as well as particularities of the interaction graph, we show that under pairwise imitation the percolation threshold is a universal indicator of how dense the occupancy ought to be for cooperation to be optimally promoted. We also demonstrate that myopic updating, due to the lack of efficient spread of information via imitation, renders the reported mechanism dysfunctional, which in turn further strengthens its foundations.Comment: 6 two-column pages, 5 figures; accepted for publication in Scientific Reports [related work available at http://arxiv.org/abs/1205.0541

Multiple acute aortic dissection in a young adult.

The diagnosis of aortic dissection in a young adult in the absence of atherosclerosis or prior history of trauma is extremely rare. The presence of more than one arterial dissection site in such a patient is even more unheard of. We highlight a case of spontaneous multiple acute arterial dissections occurring in a 32-year-old male. Stanford B aortic dissection and a separate dissection extending from the bifurcation of the right common iliac artery to the right common femoral artery was noted on computed tomographic angiography (CTA). A small aneurysm of the right subclavian artery was also noted. A two-stage hybrid procedure involving a combination of open and endovascular surgery was employed. The rarity and lethality of this condition warrants a high index of suspicion for early diagnosis and prompt intervention

Business cycles, international trade and capital flows: Evidence from Latin America

This paper adopts a flexible framework to assess both short- and long-run business cycle linkages between six Latin American (LA) countries and the four largest economies in the world (namely the US, the Euro area, Japan and China) over the period 1980:I-2011:IV. The result indicate that within the LA region there are considerable differences between countries, success stories coexisting with extremely vulnerable economies. They also show that the LA region as a whole is largely dependent on external developments, especially in the years after the great recession of 2008 and 2009. The trade channel appears to be the most important source of business cycle comovement, whilst capital flows are found to have a limited role, especially in the very short run

Risk factors for peritoneal dialysis catheter failure in children

Background Peritoneal dialysis catheter (PDC) failure still remains a common clinical problem in pediatric patients despite advancements in catheter placement and dialysis techniques. Our aim was to determine the risk factors that may lead to PDC failure, especially those factors that could be potentially modified to minimize PDC failures.Patients and methods This study was designed as a retrospective chart review of 31 patients less than 12 years of age who had end-stage renal disease (ESRD) on whom a total of 54 operative PDC placements were carried out at the tertiary Children’s Hospital, King Fahad Medical City, Riyadh, Saudi Arabia, from January 2007 to December 2010. The data included patient demographics and perioperative and operative variables.Results Fifty-four PDCs were inserted in 31 pediatric patients with ESRD, of whom 17 (55%) were boys and 14 (45%) were girls. Young age showed a statistically significant effect on PDC failure [1.8 (± 5) vs. 5 (± 7.8), P = 0.007], whereas weight did not (P = 0.085). Five types of PDCs were used, which showed significant association with PDC failure (P =0.009). Supraumbilical paramedian abdominal entry incisions were used in 49 (90.7%) patients without peritoneal leakage in any case. Nonsimultaneous omentectomy and upward PDC exit site orientation showed significant association with PDC failure (Pr0.001). The causes of PDC failure included idiopathic peritonitis in 13 (56.5%), PDC occlusion by omentum in five (21.7%), PDC malposition in four (17.4%) patients, and PDC leakage in one (4.4%) patient. Peritonitis showed a high statistical significance in PDC failure with P value of less than 0.001. The serum albumin level at the time of PDC insertion was not statistically significant in terms of PDC failure (P = 0.40) but had a high association with idiopathic peritonitis.Conclusion Our study provides some recommendations to minimize PDC failures that include improvement of patients’ nutritional status, use of a swan-neck double-cuffed catheter, paramedian abdominal entry incision, simultaneous omentectomy, downward orientation of exit site, and use of an up-to-date technique by a dedicated team for proper use of PDC. However, prospective studies possibly on a multicentric basis are necessary to standardize the best PDC insertion and maintenance techniques to minimize PDC failures and improve the quality of life for children with ESRD. Keywords: end-stage renal disease, pediatric patients, peritoneal dialysis, tenckhoff cathete