The effects of 5-fluorouracil and interferon-alpha on early healing of experimental intestinal anastomoses.

The continuing search for effective adjuvant therapy after resection of intestinal malignancies has prompted a growing interest in both immediate post-operative regional chemotherapy and the combination of 5-fluorouracil (5-FU) and interferon-alpha as drugs of choice. We have compared the effects of both compounds, alone and together, on early healing of intestinal anastomoses. Four groups (n = 26 each) of rats underwent resection and anastomosis of both ileum and colon: a control group and three groups receiving intraperitoneal 5-FU, interferon-alpha or both on the day of surgery and the next 2 days. Animals were killed 3 or 7 days (n = 10 each) after operation in order to measure anastomotic strength and hydroxyproline content. The remaining six animals in each group were used to study anastomotic collagen synthetic capacity at day 3. Three days after operation, ileal anastomotic bursting pressure was lowered by 37% in the 5-FU/interferon-alpha group (P = 0.0104). At day 7, anastomotic breaking strength was reduced significantly in ileum (P = 0.0221) and colon (P = 0.0054) of the 5-FU/interferon-alpha group and in colon of the interferon-alpha group (P = 0.0221). Collagen synthetic capacity was strongly suppressed by 5-FU but not by interferon-alpha. However, no differences in anastomotic hydroxyproline content were observed between groups at both days 3 and 7. Thus, post-operative use of interferon-alpha, in particular in combination with 5-FU, may be detrimental to anastomotic repair in the intestine

A Survey of Numerical Solutions to the Coagulation Equation

We present the results of a systematic survey of numerical solutions to the coagulation equation for a rate coefficient of the form A_ij \propto (i^mu j^nu + i^nu j^mu) and monodisperse initial conditions. The results confirm that there are three classes of rate coefficients with qualitatively different solutions. For nu \leq 1 and lambda = mu + nu \leq 1, the numerical solution evolves in an orderly fashion and tends toward a self-similar solution at large time t. The properties of the numerical solution in the scaling limit agree with the analytic predictions of van Dongen and Ernst. In particular, for the subset with mu > 0 and lambda < 1, we disagree with Krivitsky and find that the scaling function approaches the analytically predicted power-law behavior at small mass, but in a damped oscillatory fashion that was not known previously. For nu \leq 1 and lambda > 1, the numerical solution tends toward a self-similar solution as t approaches a finite time t_0. The mass spectrum n_k develops at t_0 a power-law tail n_k \propto k^{-tau} at large mass that violates mass conservation, and runaway growth/gelation is expected to start at t_crit = t_0 in the limit the initial number of particles n_0 -> \infty. The exponent tau is in general less than the analytic prediction (lambda + 3)/2, and t_0 = K/[(lambda - 1) n_0 A_11] with K = 1--2 if lambda > 1.1. For nu > 1, the behaviors of the numerical solution are similar to those found in a previous paper by us. They strongly suggest that there are no self-consistent solutions at any time and that runaway growth is instantaneous in the limit n_0 -> \infty. They also indicate that the time t_crit for the onset of runaway growth decreases slowly toward zero with increasing n_0.Comment: 41 pages, including 14 figures; accepted for publication in J. Phys.

Non-invasive Approaches for the Diagnosis of Autoimmune/Autoinflammatory Skin Diseases—A Focus on Psoriasis and Lupus erythematosus

The traditional diagnostic gold standard for inflammatory skin lesions of unclear etiology is dermato-histopathology. As this approach requires an invasive skin biopsy, biopsy processing and analysis by specialized histologists, it is a resource intensive approach requiring trained healthcare professionals. In many health care settings access to this diagnostic approach can be difficult and outside emergency cases will usually take several weeks. This scenario leads to delayed or inappropriate treatment given to patients. With dramatically increased sensitivity of a range of analysis systems including mass spectrometry, high sensitivity, multiplex ELISA based systems and PCR approaches we are now able to “measure” samples with unprecedented sensitivity and accuracy. Other important developments include the long-term monitoring of parameters using microneedle approaches and the improvement in imaging systems such as optical coherence tomography. In this review we will focus on recent achievements regarding measurements from non-invasive sampling, in particular from plucked hair and skin tape-strips which seem well suited for the diagnosis of lupus erythematosus and psoriatic inflammation, respectively. While these approaches will not replace clinical observation—they can contribute to improved subgroup diagnosis, stratified therapeutic approaches and have great potential for providing molecular and mechanistic insight in to inflammatory skin diseases.With dramatically increased sensitivity of a range of analysis systems including mass spectrometry, high sensitivity, multiplex ELISA based systems and PCR approaches we are now able to “measure” samples with unprecedented sensitivity and accuracy. Other important developments include the long-term monitoring of parameters using microneedle approaches and the improvement in imaging systems such as optical coherence tomography. In this review we will focus on recent achievements regarding measurements from non-invasive sampling, in particular from plucked hair and skin tape-strips which seem well suited for the diagnosis of lupus erythematosus and psoriatic inflammation, respectively. While these approaches will not replace clinical observation – they can contribute to improved subgroup diagnosis, stratified therapeutic approaches and have great potential for providing molecular and mechanistic insight in to inflammatory skin disease

Combined preoperative irradiation and direct postoperative 5-fluorouracil without negative effects on early anastomotic healing in the rat colon

Contains fulltext : 23545___.PDF (publisher's version ) (Open Access

Damage functions for the vulnerability assessment of masonry buildings subjected to tunneling

This paper describes a new framework for the assessment of potential damage caused by tunneling-induced settlement to surface masonry buildings. Finite element models in two and three dimensions, validated through comparison with experimental results and field observations, are used to investigate the main factors governing the structural response to settlement. Parametric analyses are performed on the effect of geometrical and structural features, like the building dimensions, the nonlinear behavior of masonry, and soil-structure interactions. These results are used to create a framework of an overall damage model that correlates the analyzed parameters with the risk of the building being damaged by a given level of settlement. The proposed vulnerability framework has the potential to be developed as a decision and management tool for the evaluation of the risk associated with underground excavations in urban areas