A survey on Movement and estimation of growth rate in banana shrimp (Penaeus merguiensis) released to the Persian Gulf and Oman sea waters (Hormuzgan province)

Banana shrimp (Penaeus merguiensis) is important shrimp species for stock enhancement in recent year in Hormozgan Tiab region. These crustacean spent post larve to juvenile cycle in mangrove creeks. Thus majore creek in this investigation are Tiab, Kolahi, Koleghan and Khamir (Laft). Maximum concentration of post larvaes in creek of Tiab, Kolahi and Koleghan are occur at April and in creek of Khamir is at May. Concentration of crustacean zoea and copepods that are diet of shrimp post larvaes, increase at this moment. Juveniles of Banana shrimp stay at those creek until June. At this time, juveniles reach to 14.6 mm (CL) and 2.9 g (TW). So optimum date to release is June for about 2.9 g of individuals. After June, concentration of juveniles decrease in creeks but increase in coastal waters. This variation in juvenile’s concentrations has negative correlation with temperature and salinity of water in creek. Amount of 187 fish stomachs (from 22 species) in the creeks were reviewed, about 56 (29.9%) of those stomachs were full or semi full conditions. Amount of 12 fish stomachs (6.4%) contain of shrimp as diet. The most important of these fish was Mangrove red snapper (Lutjanus argentimaculatus). About of 104789 juveniles of Banana shrimp (1.4g TW) in 2007 and 51401 juveniles (1.3g TW) in 2008 were tagged and released from 23 June to 16 July each year. Most of the shrimp (tagged and not tagged) released in Tiab creek. For recapture of tagged shrimp used 386 trawl survey and about 49.3 Km2 swepted by wooden boats. During the catch season (October to early November) about 10% of wooden boats and some shrimp processing companies were search daily for tagged shrimp, but no tagged shrimp were found

Antimicrobial de-escalation in the critically ill patient and assessment of clinical cure: the DIANA study

Purpose: The DIANA study aimed to evaluate how often antimicrobial de-escalation (ADE) of empirical treatment is performed in the intensive care unit (ICU) and to estimate the effect of ADE on clinical cure on day 7 following treatment initiation. Methods: Adult ICU patients receiving empirical antimicrobial therapy for bacterial infection were studied in a prospective observational study from October 2016 until May 2018. ADE was defined as (1) discontinuation of an antimicrobial in case of empirical combination therapy or (2) replacement of an antimicrobial with the intention to narrow the antimicrobial spectrum, within the first 3 days of therapy. Inverse probability (IP) weighting was used to account for time-varying confounding when estimating the effect of ADE on clinical cure. Results: Overall, 1495 patients from 152 ICUs in 28 countries were studied. Combination therapy was prescribed in 50%, and carbapenems were prescribed in 26% of patients. Empirical therapy underwent ADE, no change and change other than ADE within the first 3 days in 16%, 63% and 22%, respectively. Unadjusted mortality at day 28 was 15.8% in the ADE cohort and 19.4% in patients with no change [p = 0.27; RR 0.83 (95% CI 0.60\u20131.14)]. The IP-weighted relative risk estimate for clinical cure comparing ADE with no-ADE patients (no change or change other than ADE) was 1.37 (95% CI 1.14\u20131.64). Conclusion: ADE was infrequently applied in critically ill-infected patients. The observational effect estimate on clinical cure suggested no deleterious impact of ADE compared to no-ADE. However, residual confounding is likely

Nurses' perceptions of aids and obstacles to the provision of optimal end of life care in ICU

Contains fulltext : 172380.pdf (publisher's version ) (Open Access

Comparison of Primary Closure, Secondary Closure, and Limberg Flap in the Surgical Treatment of Pilonidal Cysts

There are few studies comparing methods for pilonidal cyst closure. This study was conducted to compare simple surgical closure, secondary healing, and closure using a Limberg flap in the treatment of patients affected with pilonidal cysts. In total, 150 patients with pilonidal cysts were selected and randomized into 3 groups, with 50 participants in each group. Group A underwent primary closure, Group B underwent secondary closure, and Group C underwent closure with a Limberg flap. Operating time, amount of pain after surgery, time to mobilization, time until return to work, time for complete healing, time until being able to sit on the toilet without pain, postoperative complications including infection and recurrence, and seroma and edema after surgery were compared among the 3 groups. Duration of surgery in the Limberg flap group was significantly longer than the duration of surgery in Group A or Group B (p <.001). The time to patient mobilization, the time when the patient returned to work, the time to complete healing, and the time to being able to sit on the toilet without pain were significantly shorter in Group C than in Group A or Group B (p <.001). Although the procedure takes longer when using a Limberg flap, it appears to be a more effective method in the surgical treatment of patients with pilonidal cyst because of the reduced healing time and improved functional status after the procedure. © 2020 Lippincott Williams and Wilkins. All rights reserved

[Improvement of the amplification rate of human chondrocytes with IGF-I and RGD].

Human chondrocytes were incubated under following conditions: Group 1 (control group): Incubation in 25 cm2 cell culture flasks (Costar) with RPMI-medium (6%-AB-serum, L-Glutamin, Hepes-buffer and antibiotics); Group 2: Different concentrations of IGF-I (1 ng/ml, 10 ng/ml) were added to the RPMI-medium; Group 3: Incubation (like control group) with additional coating of the cell culture flasks with different concentrations of RGD (5 mg/ml; 7.5 mg/ml; 10 mg/ml; 20 mg/ml); Group 4: Combination of coating with RGD (5 mg/ml; 10 mg/ml) and addition of IGF-I (1 ng/ml; 10 ng/ml) to the medium. The cells of the control group could be doubled within 2 weeks. The amplification rate of the groups 2 and 3 was improved in comparison to group 1 with the following maxima: Group 2 (5 mg/ml RGD) 3.1 times and group 3 (1 ng/ml IGF-I) 2.6 times of the number of the cells in the beginning. Group 4 (RGD and IGF-I) showed additive effects, for 4.1 times of the number of the cells in the beginning could be counted after 14 days. RGD and IGF-I (groups 2 to 4) made possible an earlier dedifferentiation and adhesion of the cells to the bottom of the cell culture flasks. By using both growth factors (RGD and IGF-I), the number of the cells could be enhanced more than 2 times in comparison to the control group within the same time. So less than half of the autologous patient's cartilage is necessary for cultivation of hyaline cartilagee