Escherichia coli and Salmonella ser. Saintpaul natural co-infection in a free-living ruddy ground dove (Columbina talpacoti): a case report

ABSTRACT This study reports a co-infection of Escherichia coli and Salmonella in a free-living ruddy ground dove (Columbina talpacoti) received at the Laboratory of Ornithological Studies of the State University of Ceará, Brazil. The bird presented diarrhea, leg paralysis and anorexia, and died shortly after. Necropsy was then performed and samples from lung, kidney, liver and intestine were collected for microbiological and histopathological analyses. Escherichia coli was isolated from cloacal swab, lung and kidney samples. Salmonella ser. Saintpaul was identified in liver and spleen samples. Escherichia coli isolates were tested for the presence of eight diagnostic genes for diarrheagenic pathotypes (STEC, ETEC, EPEC, EIEC, EAEC) with conventional polymerase chain reaction (PCR). EAEC was detected in the lung and kidney, and STEC in the intestine. In conclusion, Columbina talpacoti is susceptible to enteroaggregative Escherichia coli and Salmonella ser. Saintpaul infection, which may have public health implications

Escherichia coli and Salmonella ser. Saintpaul natural co-infection in a free-living ruddy ground dove (Columbina talpacoti): a case report

<div><p>ABSTRACT This study reports a co-infection of Escherichia coli and Salmonella in a free-living ruddy ground dove (Columbina talpacoti) received at the Laboratory of Ornithological Studies of the State University of Ceará, Brazil. The bird presented diarrhea, leg paralysis and anorexia, and died shortly after. Necropsy was then performed and samples from lung, kidney, liver and intestine were collected for microbiological and histopathological analyses. Escherichia coli was isolated from cloacal swab, lung and kidney samples. Salmonella ser. Saintpaul was identified in liver and spleen samples. Escherichia coli isolates were tested for the presence of eight diagnostic genes for diarrheagenic pathotypes (STEC, ETEC, EPEC, EIEC, EAEC) with conventional polymerase chain reaction (PCR). EAEC was detected in the lung and kidney, and STEC in the intestine. In conclusion, Columbina talpacoti is susceptible to enteroaggregative Escherichia coli and Salmonella ser. Saintpaul infection, which may have public health implications.</p></div

Detecção do efeito da adubação nitrogenada em Brachiaria decumbens Stapf. utilizando um sistema de sensoriamento remoto Detection of the nitrogen fertilization effect in Brachiaria decumbens using a remote sensing system

Objetivou-se com este trabalho utilizar técnicas de sensoriamento remoto para detectar diferentes status nutricionais em pastagem de Brachiaria decumbens Stapf. Os tratamentos consistiram de cinco doses de nitrogênio (0, 50, 100, 150 e 200 kg ha-1), com seis repetições, avaliadas em delineamento inteiramente casualizado. Foi utilizado um sistema de sensoriamento remoto composto por câmeras digitais, cabos, placa de digitalização de imagens e microcomputador, com uma haste de três metros de altura para posicionamento das câmeras. O sistema adquiriu imagens em duas bandas espectrais simultaneamente em duas fases. A primeira fase foi aos 15, 21 e 32 dias após a adubação, realizada no período de fevereiro a março de 2006, e a segunda aos 28, 36, 45 e 53 dias após a reaplicação das doses de N, no período de março a maio de 2006. Das imagens originais foram determinados diferentes índices de vegetação, os quais foram submetidos às análises de regressão e correlação. Também foram adquiridos valores estimados do teor de clorofila pelo medidor de clorofila SPAD 502 e valores do teor de N foliar. Foi possível ajustar modelos de primeiro ou segundo grau aos dados experimentais para todos os períodos estudados. Os índices calculados com a banda verde mostraram-se mais eficientes na relação com a estimativa de clorofila foliar, com o teor de N foliar e com a produtividade de matéria seca do que aqueles que utilizaram a banda vermelha, em todos os períodos estudados. Dessa maneira, a técnica de sensoriamento remoto utilizada permitiu a detecção dos diferentes efeitos das doses de nitrogênio na forrageira.<br>The objective of the present work was the detection of different nutritional statuses in Brachiaria decumbens pasture using remote sensing techniques. The area was treated with five rates of nitrogen fertilizer (0, 50, 100, 150 and, 200 kg ha-1) with six repetitions and evaluated in a completely randomized statistical design. A remote sensing system composed of digital cameras, cables, a framegrabber and a computer was used with a three meter metallic support to position the cameras. The system acquired images in two spectral bands simultaneously in two phases. The first phase occurred from February to March 2006 at 15, 21 and, 32 days after fertilization and the second from March to May of 2006 at 28, 36, 45 and, 53 days after reapplication of the same N rates. Vegetation indices were evaluated from the original images, and the data was submitted to regression and correlation analyses. Estimate values of chlorophyll content using the chlorophyll meter SPAD 502 and values of the leaf N content were also acquired. First or second degree models were adjusted to the experimental data for all periods. The indices using the green band proved more efficient to detect the relationship with the estimated chlorophyll values, the leaf N content and the dry mass yield than the red band in all studied periods. Thus, the used remote sensing system technique allowed for the identification of different effects of nitrogen fertilization in the forage