Interaction energy functional for lattice density functional theory: Applications to one-, two- and three-dimensional Hubbard models

The Hubbard model is investigated in the framework of lattice density functional theory (LDFT). The single-particle density matrix $\gamma_{ij}$ with respect the lattice sites is considered as the basic variable of the many-body problem. A new approximation to the interaction-energy functional $W[\gamma]$ is proposed which is based on its scaling properties and which recovers exactly the limit of strong electron correlations at half-band filling. In this way, a more accurate description of $W$ is obtained throughout the domain of representability of $\gamma_{ij}$, including the crossover from weak to strong correlations. As examples of applications results are given for the ground-state energy, charge-excitation gap, and charge susceptibility of the Hubbard model in one-, two-, and three-dimensional lattices. The performance of the method is demonstrated by comparison with available exact solutions, with numerical calculations, and with LDFT using a simpler dimer ansatz for $W$. Goals and limitations of the different approximations are discussed.Comment: 25 pages and 8 figures, submitted to Phys. Rev.

The Effect of Pre-Harvest Application of Pectic Oligosaccharides and Abscisic Acid on Technological Ripening and Anthocyanin Profile of ‘Syrah’ Must and Grapes Grown in a Warm Climate

The progressive increase of environmental temperature as a consequence of climate change is a challengefor the wine industry. Elevated temperatures during grape ripening affect the development of grape skincolor by inhibiting the synthesis of pigments and promoting their degradation, which causes an imbalancein the chromatic quality of must and red wine. The application of pectic oligosaccharides (POs) and abscisicacid (ABA) triggers the phenylpropanoid pathway and increases the color index in grapes. Since the atharvestpigments and phenolic compounds are determinant for wine quality, this work addressed the preharvestapplication of POs and ABA as an in-field strategy for improving the quality of Syrah must andgrapes grown in a warm climate. The color development, physicochemical parameters, phenolic content,and pigments in berries and must were evaluated. Results showed POs and ABA improved berry colordevelopment and anthocyanin content during ripening. Musts from POs-treated berries exhibited thehighest phenols concentration and the most intense color, related to higher chroma values and anthocyanincontent, particularly delphinidin, petunidin, and malvidin 3-glucosides, while ABA improved must tonalityby reducing the hue angle. In summary, POs and ABA application at veráison, differentially modulatedthe technological ripening of Syrah grapes and can be an alternative to conventional agrochemicals topreserve the quality of musts elaborated from grapes grown in warm climates, by increasing the content ofphenolic compounds and enhancing berry skin color development through the differential accumulationof anthocyanins

Discriminación de bosques de Araucaria araucana en el Parque Nacional Conguillío, centro-sur de Chile, mediante datos Landsat TM

Martin, JS (San Martin, Jose). Univ Talca, Inst Biol Vegetal & Biotecnol, Talca, ChileAraucaria forests (Araucaria araucana) have a tremendous ecological relevance; however, the information concerning their spatial distribution is still insufficient. They have only been classified according to small management scales, using satellite photos and images processed through conventional methods. The present study had as its objective to discriminate and characterize types of A. araucana forests in the Conguillio National Park, located in the Southern-Center Chile, through data derived from the Landsat-5 TM satellite and geographic information systems. The normalized difference vegetation index (NDVI) was satisfactorily related with variables corresponding to crown coverage and the diameter at breast height; thus, these index values were incorporated to the classification process. Using the digital elevation model and the NDVI, the effect provoked by the shadow was minimized. Seven types of forests, between dense and semi-dense-open, were discriminated in accordance with the accompanying species. The global reliability of the classification was 83.8 %. The greatest reliability for the producer was for the medium crown density forest of A. araucana - N. dombeyi (B1) (87.5 %); and for the consumer, for the high crown density forests of A. araucana - N. dombeyi (B1) and also for those of medium density (B2) (93 %). It is concluded that incorporating NDVI values and data derived from the digital elevation model to the satellite classification process, it is possible to discriminate araucaria forests with satisfactory reliability in areas of rough relief, which is very useful information for the management of these forestry ecosystems

Modelo procedimental de la evaluación de las políticas públicas a través de la ludificación / Procedural model in the evaluation of public policy through gamification

This article proposes a procedural model designed to optimize the experience of citizens in decision making of public policies through gamification. The study assumes that gamification is motivating and contributes to increase involvement within a specific area. In this case, gamification is used to encourage citizens’ engagement in decision-making, based on three models of public policies evaluation that incorporate civic participation in three different roles: auditor, passive and active. Applying the Delphi methodology, the results of reliability and internal consistency show that experts attribute higher value to the model of active participation using gamification. In conclusion, the model designed opens a new area of knowledge within public policies evaluation, embedded in the interactivity of the media contex

La opinión de Feaps sobre el proceso de inclusión educativa

En este texto se presenta de forma resumida el diseño y algunos resultados de una investigación que tuvo como objetivo conocer la opinión de las organizaciones de personas con discapacidad en España, representadas en el CERMI, sobre el desarrollo del proceso de inclusión en nuestro país. El análisis se ha realizado desde la perspectiva de los técnicos o colaboradores de dichas organizaciones, quienes aportan su conocimiento experto en los temas educativos abordados. Se presentan los principales resultados que emanan de la consulta a la Confederación Española de Organizaciones a favor de las Personas con Discapacidad Intelectual (FEAPS) junto con las conclusiones generales del proyecto. Los resultados generales muestran progresos significativos en las dimensiones evaluadas, pero también la persistencia de importantes barreras que limitan la igualdad de oportunidades de los estudiantes con discapacidad. La situación de los niños y jóvenes con discapacidad intelectual es peor valorada, en general, que la de otros grupos analizados

Id2 leaves the chromatin of the E2F4-p130-controlled c-myc promoter during hepatocyte priming for liver regeneration

The Id (inhibitor of DNA binding or inhibitor of differentiation) helix-loop-helix proteins are involved in the regulation of cell growth, differentiation and cancer. The fact that the molecular mechanisms of liver regeneration are not completely understood prompted us to study the fate of Id2 in proliferating liver. Id2 increases in liver regeneration after partial hepatectomy, following the early induction of its gene. Co-immunoprecipitation shows that Id2 forms a complex with E2F4, p130 and mSin3A in quiescent liver and all these components are present at the c-myc promoter as shown using ChIP (chromatin immunoprecipitation). Activation of c-myc during hepatocyte priming (G0-G1 transition) correlates with the dissociation of Id2 and HDAC (histone deacetylase), albeit p130 remains bound at least until 6 h. Moreover, as the G0-G1 transition progresses, Id2 and HDAC again bind the c-myc promoter concomitantly with the repression of this gene. The time course of c-myc binding to the Id2 promoter, as determined by ChIP assays is compatible with a role of the oncoprotein as a transcriptional inducer of Id2 in liver regeneration. Immunohistochemical analysis shows that Id2 also increases in proliferating hepatocytes after bile duct ligation. In this case, the pattern of Id2 presence in the c-myc promoter parallels that found in regenerating liver. Our results may suggest a control role for Id2 in hepatocyte priming, through a p130 dissociation-independent regulation of c-my

A Comprehensive Analysis of Choroideremia: From Genetic Characterization to Clinical Practice.

Choroideremia (CHM) is a rare X-linked disease leading to progressive retinal degeneration resulting in blindness. The disorder is caused by mutations in the CHM gene encoding REP-1 protein, an essential component of the Rab geranylgeranyltransferase (GGTase) complex. In the present study, we evaluated a multi-technique analysis algorithm to describe the mutational spectrum identified in a large cohort of cases and further correlate CHM variants with phenotypic characteristics and biochemical defects of choroideremia patients. Molecular genetic testing led to the characterization of 36 out of 45 unrelated CHM families (80%), allowing the clinical reclassification of four CHM families. Haplotype reconstruction showed independent origins for the recurrent p.Arg293* and p.Lys178Argfs*5 mutations, suggesting the presence of hotspots in CHM, as well as the identification of two different unrelated events involving exon 9 deletion. No certain genotype-phenotype correlation could be established. Furthermore, all the patients´ fibroblasts analyzed presented significantly increased levels of unprenylated Rabs proteins compared to control cells; however, this was not related to the genotype. This research demonstrates the major potential of the algorithm proposed for diagnosis. Our data enhance the importance of establish a differential diagnosis with other retinal dystrophies, supporting the idea of an underestimated prevalence of choroideremia. Moreover, they suggested that the severity of the disorder cannot be exclusively explained by the genotype

Dissection-independent production of Plasmodium sporozoites from whole mosquitoes

Progress towards a protective vaccine against malaria remains slow. To date, only limited protection has been routinely achieved following immunisation with either whole-parasite (sporozoite) or subunit-based vaccines. One major roadblock to vaccine progress, and to pre-erythrocytic parasite biology in general, is the continued reliance on manual salivary gland dissection for sporozoite isolation from infected mosquitoes. Here, we report development of a multi-step method, based on batch processing of homogenised whole mosquitoes, slurry, and density-gradient filtration, which combined with free -flow electrophoresis rapidly produces a pure, infective sporozoite inoculum. Human-infective Plasmodium falciparum and rodent-infective Plasmodium berghei sporozoites produced in this way are two-to threefold more infective than salivary gland dissection sporozoites in in vitro hepatocyte infection assays. In an in vivo rodent malaria model, the same P. berghei sporozoites confer sterile protection from mosquito-bite challenge when immunisation is delivered intravenously or 60-70% protection when delivered intramuscularly. By improving purity, infectivity, and immunogenicity, this method represents a key advancement in capacity to produce research grade sporozoites, which should impact delivery of a whole parasite based malaria vaccine at scale in the future.Host-parasite interactio

Associations of Anti-COVID-19 Measures and Lifestyle Changes during the COVID-19 Pandemic with Sleep Patterns in the Netherlands: A Longitudinal Study

Although there is scientific evidence for an increased prevalence of sleep disorders during the coronavirus disease 2019 (COVID-19) pandemic, there is still limited information on how lifestyle factors might have affected sleep patterns. Therefore, we followed a large cohort of participants in the Netherlands (n = 5,420) for up to 1 year (September 2020-2021) via monthly Web-based questionnaires to identify lifestyle changes (physical activity, cigarette smoking, alcohol consumption, electronic device use, and social media use) driven by anti-COVID-19 measures and their potential associations with self-reported sleep (latency, duration, and quality). We used the Containment and Health Index (CHI) to assess the stringency of anti-COVID-19 measures and analyzed associations through multilevel ordinal response models. We found that more stringent anti-COVID-19 measures were associated with higher use of electronic devices (per interquartile-range increase in CHI, odds ratio (OR) = 1.47, 95% confidence interval (CI): 1.40, 1.53), less physical activity (OR = 0.94, 95% CI: 0.90, 0.98), lower frequency of alcohol consumption (OR = 0.63, 95% CI: 0.60, 0.66), and longer sleep duration (OR = 1.11, 95% CI: 1.05, 1.16). Lower alcohol consumption frequency and higher use of electronic devices and social media were associated with longer sleep latency. Lower physical activity levels and higher social media and electronic device use were related to poorer sleep quality and shorter sleep duration

Effectiveness of EDACS Versus ADAPT Accelerated Diagnostic Pathways for Chest Pain: A Pragmatic Randomized Controlled Trial Embedded Within Practice

Study objective A 2-hour accelerated diagnostic pathway based on the Thrombolysis in Myocardial Infarction score, ECG, and troponin measures (ADAPT-ADP) increased early discharge of patients with suspected acute myocardial infarction presenting to the emergency department compared with standard care (from 11% to 19.3%). Observational studies suggest that an accelerated diagnostic pathway using the Emergency Department Assessment of Chest Pain Score (EDACS-ADP) may further increase this proportion. This trial tests for the existence and size of any beneficial effect of using the EDACS-ADP in routine clinical care. Methods This was a pragmatic randomized controlled trial of adults with suspected acute myocardial infarction, comparing the ADAPT-ADP and the EDACS-ADP. The primary outcome was the proportion of patients discharged to outpatient care within 6 hours of attendance, without subsequent major adverse cardiac event within 30 days. Results Five hundred fifty-eight patients were recruited, 279 in each arm. Sixty-six patients (11.8%) had a major adverse cardiac event within 30 days (ADAPT-ADP 29; EDACS-ADP 37); 11.1% more patients (95% confidence interval 2.8% to 19.4%) were identified as low risk in EDACS-ADP (41.6%) than in ADAPT-ADP (30.5%). No low-risk patients had a major adverse cardiac event within 30 days (0.0% [0.0% to 1.9%]). There was no difference in the primary outcome of proportion discharged within 6 hours (EDACS-ADP 32.3%; ADAPT-ADP 34.4%; difference −2.1% [−10.3% to 6.0%], P=.65). Conclusion There was no difference in the proportion of patients discharged early despite more patients being classified as low risk by the EDACS-ADP than the ADAPT-ADP. Both accelerated diagnostic pathways are effective strategies for chest pain assessment and resulted in an increased rate of early discharges compared with previously reported rates