Outstanding intraindividual genetic diversity in fissiparous planarians (Dugesia, Platyhelminthes) with facultative sex.

Predicted genetic consequences of asexuality include high intraindividual genetic diversity (i.e., the Meselson effect) and accumulation of deleterious mutations (i.e., Muller’s Ratchet), among others. These consequences have been largely studied in parthenogenetic organisms, but studies on fissiparous species are scarce. Differing from parthenogens, fissiparous organisms inherit part of the soma of the progenitor, including somatic mutations. Thus, in the long term, fissiparous reproduction may also result in genetic mosaicism, besides the presence of the Meselson effect and Muller’s Ratchet. Dugesiidae planarians show outstanding regeneration capabilities, allowing them to naturally reproduce by fission, either strictly or combined with sex (facultative). Therefore, they are an ideal model to analyze the genetic footprint of fissiparous reproduction, both when it is alternated with sex and when it is the only mode of reproduction

Evidence for an ecological cost of enhanced herbicide metabolism in Lolium rigidum

1. In some cases, evaluation of resource competitive interactions between herbicide resistant vs. susceptible weed ecotypes provides evidence for the expression of fitness costs associated with evolved herbicide-resistant gene traits. Such fitness costs impact in the ecology and evolutionary trajectory of resistant populations. 2. Neighbourhood experiments were performed to quantify competitive effects and responses between herbicide-susceptible (S) and resistant (R) Lolium rigidum individuals in which resistance is due to enhanced herbicide metabolism mediated by cytochrome P450. 3. In two-way competitive interactions between the S and R phenotypes, individuals of the S phenotype were the stronger effect competitors on both a per capita and per unit-size basis. The S phenotype also exhibited a stronger competitive response to wheat plants than did the R phenotype, displaying significantly greater (30%) above-ground biomass at the vegetative stage. When subjected to competition from wheat, R individuals produced significantly fewer reproductive tillers and allocated fewer resources to reproductive traits than individuals of the S phenotype. 4. The role of potential mechanisms underlying this resistance cost driven by traits such as plant size and tolerance to low resource availability, as well as the evolutionary implications of the results are discussed. 5. Synthesis. Evolved herbicide resistance due to enhanced-herbicide metabolism mediated by cytochrome-P450 in L. rigidum has been shown to be accompanied with an impaired ability to compete for resources. These results are consistent with the resource-based theory that predicts a negative trade-off between growth and plant defence

Fitness costs associated with evolved herbicide resistance alleles in plants

Predictions based on evolutionary theory suggest that the adaptive value of evolved herbicide resistance alleles may be compromised by the existence of fitness costs. There have been many studies quantifying the fitness costs associated with novel herbicide resistance alleles, reflecting the importance of fitness costs in determining the evolutionary dynamics of resistance. However, many of these studies have incorrectly defined resistance or used inappropriate plant material and methods to measure fitness. This review has two major objectives. First, to propose a methodological framework that establishes experimental criteria to unequivocally evaluate fitness costs. Second, to present a comprehensive analysis of the literature on fitness costs associated with herbicide resistance alleles. This analysis reveals unquestionable evidence that some herbicide resistance alleles are associated with pleiotropic effects that result in plant fitness costs. Observed costs are evident from herbicide resistance-endowing amino acid substitutions in proteins involved in amino acid, fatty acid, auxin and cellulose biosynthesis, as well as enzymes involved in herbicide metabolism. However, these resistance fitness costs are not universal and their expression depends on particular plant alleles and mutations. The findings of this review are discussed within the context of the plant defence trade-off theory and herbicide resistance evolution

O VI and Multicomponent H I Absorption Associated with a Galaxy Group in the Direction of PG0953+415: Physical Conditions and Baryonic Content

We report the discovery of an O VI absorption system at z(abs) = 0.14232 in a high resolution FUV spectrum of PG0953+415 obtained with the Space Telescope Imaging Spectrograph (STIS). Both lines of the O VI 1032, 1038 doublet and multicomponent H I Lya absorption are detected, but the N V doublet and the strong lines of C II and Si III are not apparent. We examine the ionization mechanism of the O VI absorber and find that while theoretical considerations favor collisional ionization, it is difficult to observationally rule out photoionization. If the absorber is collisionally ionized, it may not be in equilibrium due to the rapid cooling of gas in the appropriate temperature range. Non-equilibrium collisionally ionized models are shown to be consistent with the observations. A WIYN survey of galaxy redshifts near the sight line has revealed a galaxy at a projected distance of 395 kpc separated by ~130 km/s from this absorber, and three additional galaxies are found within 130 km/s of this redshift with projected separations ranging from 1.0 Mpc to 3.0 Mpc. Combining the STIS observations of PG0953+415 with previous high S/N GHRS observations of H1821+643, we derive a large number of O VI absorbers per unit redshift, dN/dz ~20. We use this sample to obtain a first estimate of the cosmological mass density of the O VI systems at z ~ 0. If further observations confirm the large dN/dz derived for the O VI systems, then these absorbers trace a significant reservoir of baryonic matter at low redshift.Comment: Accepted for publication in Ap.J., vol. 542 (Oct. 10, 2000

Parametrization of gamma-ray production cross-sections for pp interactions in a broad proton energy range from the kinematic threshold to PeV energies

Using publicly available Monte Carlo codes as well as compilation of published data on p--p interactions for proton kinetic energy below 2 GeV, we parametrize the energy spectra and production rates of $\gamma$-rays by simple but quite accurate ($\leq 20 \%$) analytical expressions in a broad range from the kinematic threshold to PeV energies.Comment: 19 pages, 15 figures, v2 version fixes the cross reference