Secondary habitats are important in biodiversity conservation: a case study on orthopterans along ditch banks

Los hábitats secundarios son importantes en la conservación de la biodiversidad: un estudio práctico sobre los ortópteros en orillas de acequias Se ha demostrado que la biota autóctona puede sobrevivir en hábitats secundarios como cunetas, diques y setos. La finalidad de este estudio es evaluar el valor de las orillas de acequias para la conservación de los ortópteros en un paisaje agrícola en Hungría, a partir del análisis de los datos relativos a la riqueza y la abundancia de especies utilizando modelos mixtos. No encontramos ninguna diferencia en cuanto a la riqueza de especies entre las orillas de acequias aisladas, semiaisladas y en praderas de control. Sin embargo, el grado de aislamiento tuvo un efecto negativo significativo en la abundancia de especies sedentarias. Constatamos que la densidad de vegetación leñosa junto a las orillas de las acequias tenía un efecto negativo en la abundancia total y la abundancia de especies móviles. Se observó la existencia de una relación positiva entre la anchura de las orillas de acequias que estaba cubierta por vegetación y la abundancia de especies del suborden Caelifera y de especies móviles, xerófilas y mesófilas. Nuestros resultados sugieren que la densidad de ortópteros puede ser una medida más sensible de la calidad del hábitat que la riqueza de especies. Concluimos que las orillas de las acequias son un hábitat adecuado para la mayoría de ortópteros, incluidas las especies raras o en peligro de extinción, lo que pone de relieve que debería prestarse más atención a estos y otros hábitats lineales parecidos y que se les debería dar más importancia en la conservación de invertebrados.It has been shown that native biota can survive in secondary habitats such as road verges, dikes and hedges. We aimed to assess the conservation value of ditch banks for orthopterans in an agricultural landscape in Hungary, based on the analyses of species richness and abundance data using mixed–models. We did not find any differences in the species richness between isolated ditch banks, semi–isolated ditch banks and control meadows. The extent of isolation had a significantly negative effect, however, on the abundance of sedentary species. We found that the density of woody vegetation along ditch banks had a negative effect on the total abundance and the abundance of mobile species. Positive relationships were found between the width of ditch bank vegetation and the abundance of Caelifera, mobile, xerophilous and mesophilous species. Our results suggest that the density of orthopterans may be a more sensitive measure for habitat quality than their species richness. We concluded that ditch banks are a suitable habitat for the majority of orthopterans, including rare and endangered species, emphasizing that ditch banks and similar linear habitats should receive more attention and should be given a more prominent role in invertebrate conservation.Los hábitats secundarios son importantes en la conservación de la biodiversidad: un estudio práctico sobre los ortópteros en orillas de acequias Se ha demostrado que la biota autóctona puede sobrevivir en hábitats secundarios como cunetas, diques y setos. La finalidad de este estudio es evaluar el valor de las orillas de acequias para la conservación de los ortópteros en un paisaje agrícola en Hungría, a partir del análisis de los datos relativos a la riqueza y la abundancia de especies utilizando modelos mixtos. No encontramos ninguna diferencia en cuanto a la riqueza de especies entre las orillas de acequias aisladas, semiaisladas y en praderas de control. Sin embargo, el grado de aislamiento tuvo un efecto negativo significativo en la abundancia de especies sedentarias. Constatamos que la densidad de vegetación leñosa junto a las orillas de las acequias tenía un efecto negativo en la abundancia total y la abundancia de especies móviles. Se observó la existencia de una relación positiva entre la anchura de las orillas de acequias que estaba cubierta por vegetación y la abundancia de especies del suborden Caelifera y de especies móviles, xerófilas y mesófilas. Nuestros resultados sugieren que la densidad de ortópteros puede ser una medida más sensible de la calidad del hábitat que la riqueza de especies. Concluimos que las orillas de las acequias son un hábitat adecuado para la mayoría de ortópteros, incluidas las especies raras o en peligro de extinción, lo que pone de relieve que debería prestarse más atención a estos y otros hábitats lineales parecidos y que se les debería dar más importancia en la conservación de invertebrados

Tree–herb co-existence and community assembly in natural forest-steppe transitions

Background: The effects of trees on understorey communities is a major driver of vegetation composition. However, we have little understanding on how isolated forest patches of the forest-steppe transition affect their herb layer as compared to adjacent grasslands. Aims: Our aim was to test whether trees had a protective effect on understorey herbaceous communities in the most arid regions of the forest-steppe transition, where the stress gradient hypothesis predicts positive net effects. Methods: We surveyed herbaceous cover and species composition in 135 forest-steppe sites and recorded soil moisture, microclimate and canopy cover in northern Kazakhstan. Results: Total cover and species richness were lower in the herb layer of groves than in the steppe stands and the soil of the groves was not moister than that of the steppes. Groves were dominated by grassland specialist plants, while forest specialist species remained scarce. However, these grassland species were different from the ones inhabiting the steppes, leading to little nestedness between groves and steppes. Conclusions: Although the presence of groves greatly increase the landscape level diversity of Middle-Asian forest-steppes, trees in the southern edge of the zone seem to have net negative effect on the understorey vegetation, seemingly contradicting the stress gradient hypothesis

Malignancy after heart transplantation: incidence, prognosis and risk factors

[Abstract] The Spanish Post-Heart-Transplant Tumour Registry comprises data on neoplasia following heart transplantation (HT) for all Spanish HT patients (1984–2003). This retrospective analysis of 3393 patients investigated the incidence and prognosis of neoplasia, and the influence of antiviral prophylaxis. About 50% of post-HT neoplasias were cutaneous, and 10% lymphomas. The cumulative incidence of skin cancers and other nonlymphoma cancers increased with age at HT and with time post-HT (from respectively 5.2 and 8.9 per 1000 person-years in the first year to 14.8 and 12.6 after 10 years), and was greater among men than women. None of these trends held for lymphomas. Induction therapy other than with IL2R-blockers generally increased the risk of neoplasia except when acyclovir was administered prophylactically during the first 3 months post-HT; prophylactic acyclovir halved the risk of lymphoma, regardless of other therapies. Institution of MMF during the first 3 months post-HT reduced the incidence of skin cancer independently of the effects of sex, age group, pre-HT smoking, use of tacrolimus in the first 3 months, induction treatment and antiviral treatment. Five-year survival rates after first tumor diagnosis were 74% for skin cancer, 20% for lymphoma and 32% for other tumors

Hypoxia increases membrane metallo-endopeptidase expression in a novel lung cancer ex vivo model - role of tumor stroma cells

Background: Hypoxia-induced genes are potential targets in cancer therapy. Responses to hypoxia have been extensively studied in vitro, however, they may differ in vivo due to the specific tumor microenvironment. In this study gene expression profiles were obtained from fresh human lung cancer tissue fragments cultured ex vivo under different oxygen concentrations in order to study responses to hypoxia in a model that mimics human lung cancer in vivo.Methods: Non-small cell lung cancer (NSCLC) fragments from altogether 70 patients were maintained ex vivo in normoxia or hypoxia in short-term culture. Viability, apoptosis rates and tissue hypoxia were assessed. Gene expression profiles were studied using Affymetrix GeneChip 1.0 ST microarrays.Results: Apoptosis rates were comparable in normoxia and hypoxia despite different oxygenation levels, suggesting adaptation of tumor cells to hypoxia. Gene expression profiles in hypoxic compared to normoxic fragments largely overlapped with published hypoxia-signatures. While most of these genes were up-regulated by hypoxia also in NSCLC cell lines, membrane metallo-endopeptidase (MME, neprilysin, CD10) expression was not increased in hypoxia in NSCLC cell lines, but in carcinoma-associated fibroblasts isolated from non-small cell lung cancers. High MME expression was significantly associated with poor overall survival in 342 NSCLC patients in a meta-analysis of published microarray datasets.Conclusions: The novel ex vivo model allowed for the first time to analyze hypoxia-regulated gene expression in preserved human lung cancer tissue. Gene expression profiles in human hypoxic lung cancer tissue overlapped with hypoxia-signatures from cancer cell lines, however, the elastase MME was identified as a novel hypoxia-induced gene in lung cancer. Due to the lack of hypoxia effects on MME expression in NSCLC cell lines in contrast to carcinoma-associated fibroblasts, a direct up-regulation of stroma fibroblast MME expression under hypoxia might contribute to enhanced aggressiveness of hypoxic cancers

Health‐related quality of life, social support, and caregiver burden between six and 120 months after heart transplantation: a Spanish multicenter cross‐sectional study

[Abstract] A multicenter cross-sectional study was conducted to determine the current heart transplant (HTx) outcomes in Spain. Clinical and functional status, health-related quality of life (HRQoL), social support, and caregiver burden were analyzed in 303 adult transplant recipients (77.9% males) living with one functioning graft. Mean age at time of HTx (SD) was 56.4 (11.4) years, and the reason for transplantation in all patients was congestive heart failure. All patients had received a first heart transplant 6 (± 1), 12 (± 2), 36 (± 6), 60 (± 10), or 120 (± 20) months previously. Participants completed the Kansas City Cardiomyopathy Questionnaire (KCCQ), the EQ-5D, the Duke-UNC Functional Social Support Questionnaire, and the Zarit Caregiver Burden Scale. Reasonable HRQoL, social support, and caregiver burden levels were found at all time points, although a slight decrease in HRQoL was recorded at 120 months (p ≤ 0.033). Multivariate regression analyses showed that complications, comorbidities, and hospitalizations were associated with HRQoL (EQ-5D: 48.4% of explained variance, F4,164 = 38.46, p < 0.001; KCCQ overall summary score: 45.0%, F3,198 = 54.073, p < 0.001). Patient functional capabilities and complications affected caregiver burden (p < 0.05). In conclusion, HTx patients reported reasonable levels of HRQoL with low caregiver burden. Clinical variables related to these outcomes included functional status, complications, and number of admissions

Exploring glycopeptide-resistance in Staphylococcus aureus: a combined proteomics and transcriptomics approach for the identification of resistance-related markers

BACKGROUND: To unravel molecular targets involved in glycopeptide resistance, three isogenic strains of Staphylococcus aureus with different susceptibility levels to vancomycin or teicoplanin were subjected to whole-genome microarray-based transcription and quantitative proteomic profiling. Quantitative proteomics performed on membrane extracts showed exquisite inter-experimental reproducibility permitting the identification and relative quantification of >30% of the predicted S. aureus proteome. RESULTS: In the absence of antibiotic selection pressure, comparison of stable resistant and susceptible strains revealed 94 differentially expressed genes and 178 proteins. As expected, only partial correlation was obtained between transcriptomic and proteomic results during stationary-phase. Application of massively parallel methods identified one third of the complete proteome, a majority of which was only predicted based on genome sequencing, but never identified to date. Several over-expressed genes represent previously reported targets, while series of genes and proteins possibly involved in the glycopeptide resistance mechanism were discovered here, including regulators, global regulator attenuator, hyper-mutability factor or hypothetical proteins. Gene expression of these markers was confirmed in a collection of genetically unrelated strains showing altered susceptibility to glycopeptides. CONCLUSION: Our proteome and transcriptome analyses have been performed during stationary-phase of growth on isogenic strains showing susceptibility or intermediate level of resistance against glycopeptides. Altered susceptibility had emerged spontaneously after infection with a sensitive parental strain, thus not selected in vitro. This combined analysis allows the identification of hundreds of proteins considered, so far as hypothetical protein. In addition, this study provides not only a global picture of transcription and expression adaptations during a complex antibiotic resistance mechanism but also unravels potential drug targets or markers that are constitutively expressed by resistant strains regardless of their genetic background, amenable to be used as diagnostic targets