128 research outputs found
Dynamic studies of antibody-antigen interactions with an electrolyte-gated organic transistor
Affinity-based biosensors employing surface-bound biomolecules
for analyte detection are important tools in clinical diagnostics
and drug development. In this context, electrolyte-gated organic
transistors (EGOTs) are emerging as ultrasensitive label-free biosensors. In this study, we present an EGOT sensor integrated
within a microfluidic system. The sensor utilizes the cytomegalovirus (CMV) phosphoprotein 65 as a biorecognition element to
detect the pathological biomarker human anti-cytomegalovirus
antibody in solution. The biorecognition element is grafted onto
the gate electrode by exploiting the polyhistidine-tag technology.
Real-time monitoring of the EGOT response, coupled with a twocompartment kinetic model analysis, enables the determination
of analyte concentration, binding kinetics, and thermodynamics
of the interaction. The analysis of the relevant kinetic parameters
of the binding process yields a reliable value for the thermodynamic equilibrium constant and suggests that the measured
deviations from the Langmuir binding model arise from the co-existence of binding sites with different affinities toward the antibodies
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Surface plasmon resonance-enhanced light interaction in an integrated ormocomp nanowire
An integrated ormocomp nanowire coated with gold metal layer is proposed and its optical characteristics with the effect of surface plasmon resonance (SPR) are studied. The integrated rib-like nanowire has a trapezoidal shape with sidewall angles of 75°. It is coated with 50 nm gold layer to introduce the SPR and enhance the evanescent field in the sensing region located at the dielectric/metal interface. The possible field modes, the normalized power confinement, and the SPR peak position of the nanowire are studied over the wavelength and the metal thickness by using the full-vectorial H-field FEM in quasi-TM mode. The attenuation coefficient of the nanowire, the SPR peak wavelength, and the wavelength shift is experimentally extracted for three different cladding materials. The redshift of the supermode coupling between the dielectric mode and the anti-symmetric supermode is observed with the higher cladding-index and larger metal thickness. The improvement of the power confinement in the sensing region with the SPR effect is ten times (10×) better than a previous similar study
Results from an Operational Demonstration of a Gridded CrIS/ATMS Product for Cold Air Aloft
No abstract availabl
Angle-Tunable Enhanced Infrared Reflection Absorption Spectroscopy via Grating-Coupled Surface Plasmon Resonance
Surface enhanced infrared absorption (SEIRA) spectroscopy is an attractive method for increasing the prominence of vibrational modes in infrared spectroscopy. To date, the majority of reports associated with SEIRA utilize localized surface plasmon resonance from metal nanoparticles to enhance electromagnetic fields in the region of analytes. Limited work has been performed using propagating surface plasmons as a method for SEIRA excitation. In this report, we demonstrate angle-tunable enhancement of vibrational stretching modes associated with a thin poly(methyl methacrylate) (PMMA) film that is coupled to a silver-coated diffraction grating. Gratings are fabricated using laser interference lithography to achieve precise surface periodicities, which can be used to generate surface plasmons that overlap with specific vibrational modes in the polymer film. Infrared reflection absorption spectra are presented for both bare silver and PMMA-coated silver gratings at a range of angles and polarization states. In addition, spectra were obtained with the grating direction oriented perpendicular and parallel to the infrared source in order to isolate plasmon enhancement effects. Optical simulations using the rigorous coupled-wave analysis method were used to identify the origin of the plasmon-induced enhancement. Angle-dependent absorption measurements achieved signal enhancements of more than 10-times the signal in the absence of the plasmon.This article is from Analytical Chemistry86 (2014): 2610-2617, doi:10.1021/ac4038398. Posted with permission.</p
Экспериментальное изучение влияния глюкозамина гидрохлорида на развитие патоспермии стареющих крыс, вызванной доксорубицином
ГЛЮКОЗАМИНА ГИДРОХЛОРИДДОКСОРУБИЦИНПАТОСПЕРМИЯСТАРЕНИЕФАРМАКОЛОГИЯ КЛИНИЧЕСКАЯЛЕКАРСТВ ФИЗИОЛОГИЧЕСКОЕ ДЕЙСТВИЕРЕПРОДУКЦИЮ КОНТРОЛИРУЮЩИЕ СРЕДСТВАЭКСПЕРИМЕНТЫ НА ЖИВОТНЫХКРЫСЫЦель работы - исследование влияния глюкозамина гидрохлорида на развитие гипофункции семенников крыс, вызванной длительным введением доксорубицина на фоне старения животных
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