113 research outputs found
Beetles (Coleoptera) of Peru: A Survey of the Families. Scarabaeoidea
The 1042 species of Scarabaeoidea known to occur in Peru are listed with their taxonomic placement in families, subfamilies, and tribes
A comparison of MITS counseling and informed consent processes in Pakistan, India, Bangladesh, Kenya, and Ethiopia
Globally, more than 5 million stillbirths and neonatal deaths occur annually. For many, the cause of death (CoD) is unknown. Minimally invasive tissue sampling (MITS) has been increasingly used in postmortem examinations for ascertaining the CoD in stillbirths and neonates. Our study compared the counseling and consent methods used in MITS projects in five countries in Africa and south Asia. Key informant interviews were conducted with researchers to describe the characteristics and backgrounds of counselors, the environment and timing of consent and perceived facilitators and barriers encountered during the consent process. Counselors at all sites had backgrounds in social science, psychology and counseling or clinical expertise in obstetrics/gynecology or pediatrics. All counsellors received training about techniques for building rapport and offering emotional support to families; training duration and methods differed across sites. Counselling environments varied significantly; some sites allocated a separate room, others counselled families at the bedside or nursing stations. All counsellors had a central role in explaining the MITS procedure to families in their local languages. Most sites did not use visual aids during the process, relying solely on verbal descriptions. In most sites, parents were approached within one hour of death. The time needed for decision making by families varied from a few minutes to 24 h. In most sites, extended family took part in the decision making. Because many parents wanted burial as soon as possible, counsellors ensured that MITS would be conducted promptly after receiving consent. Barriers to consent included decreased comprehension of information due to the emotional and psychological impact of grief. Moreover, having more family members engaged in decision-making increased the complexity of counselling and achieving consensus to consent for the procedure. While each site adapted their approach to fit the context, consistencies and similarities across sites were observed
Intravenous Sodium Thiosulphate for Calciphylaxis of Chronic Kidney Disease:A Systematic Review and Meta-analysis
Importance: Calciphylaxis is a rare disease with high mortality mainly involving patients with chronic kidney disease (CKD). Sodium thiosulphate (STS) has been used as an off-label therapeutic in calciphylaxis, but there is a lack of clinical trials and studies that demonstrate its effect compared with those without STS treatment. Objective: To perform a meta-analysis of the cohort studies that provided data comparing outcomes among patients with calciphylaxis treated with and without intravenous STS. Data Sources: PubMed, Embase, Cochrane Library, Web of Science, and ClinicalTrials.gov were searched using relevant terms and synonyms including sodium thiosulphate and calci∗ without language restriction. Study Selection: The initial search was for cohort studies published before August 31, 2021, that included adult patients diagnosed with CKD experiencing calciphylaxis and could provide a comparison between patients treated with and without intravenous STS. Studies were excluded if they reported outcomes only from nonintravenous administration of STS or if the outcomes for CKD patients were not provided. Data Extraction and Synthesis: Random-effects models were performed. The Egger test was used to measure publication bias. Heterogeneity was assessed using the I2test. Main Outcomes and Measures: Skin lesion improvement and survival, synthesized as ratio data by a random-effects empirical Bayes model. Results: Among the 5601 publications retrieved from the targeted databases, 19 retrospective cohort studies including 422 patients (mean age, 57 years; 37.3% male) met the eligibility criteria. No difference was observed in skin lesion improvement (12 studies with 110 patients; risk ratio, 1.23; 95% CI, 0.85-1.78) between the STS and the comparator groups. No difference was noted for the risk of death (15 studies with 158 patients; risk ratio, 0.88; 95% CI, 0.70-1.10) and overall survival using time-to-event data (3 studies with 269 participants; hazard ratio, 0.82; 95% CI, 0.57-1.18). In meta-regression, lesion improvement associated with STS negatively correlated with publication year, implying that recent studies are more likely to report a null association compared with past studies (coefficient = -0.14; P =.008). Conclusions and Relevance: Intravenous STS was not associated with skin lesion improvement or survival benefit in patients with CKD experiencing calciphylaxis. Future investigations are warranted to examine the efficacy and safety of therapies for patients with calciphylaxis.</p
Malagasy bats shelter a considerable genetic diversity of pathogenic Leptospira suggesting notable host-specificity patterns
Pathogenic Leptospira are the causative agents of leptospirosis, a disease of global concern with major impact in tropical regions. Despite the importance of this zoonosis for human health, the evolutionary and ecological drivers shaping bacterial communities in host reservoirs remain poorly investigated. Here, we describe Leptospira communities hosted by Malagasy bats, composed of mostly endemic species, in order to characterize host-pathogen associations and investigate their evolutionary histories. We screened 947 individual bats (representing 31 species, 18 genera and seven families) for Leptospira infection and subsequently genotyped positive samples using three different bacterial loci. Molecular identification showed that these Leptospira are notably diverse and include several distinct lineages mostly belonging to Leptospira borgpetersenii and L. kirschneri. The exploration of the most probable host-pathogen evolutionary scenarios suggests that bacterial genetic diversity results from a combination of events related to the ecology and the evolutionary history of their hosts. Importantly, based on the data set presented herein, the notable host-specificity we have uncovered, together with a lack of geographical structuration of bacterial genetic diversity, indicates that the Leptospira community at a given site depends on the co-occurring bat species assemblage. The implications of such tight host-specificity on the epidemiology of leptospirosis are discussed.http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1574-69412017-06-30hb2016Microbiology and Plant Patholog
An integrative approach to characterize Malagasy bats of the subfamily Vespertilioninae Gray, 1821, with the description of a new species of Hypsugo
Although important advances have been made in recent years in the taxonomy of different families and subfamilies
of Malagasy bats, those belonging to the Vespertilioninae remain partially unresolved. Herein using a mitochondrial
marker (cytochrome b) as the point of departure for 76 specimens of Malagasy vespers and appropriate African
taxa, we diagnose the six taxa of this subfamily on the island by overlaying different morphological and bioacoustic
characters on the clade structure of sequenced animals. The species include: endemic Neoromicia matroka, which
is sister to African N. capensis; endemics N. malagasyensis and N. robertsi, which form sister species; a member
of the genus Hypsugo, which is sister to African H. anchietae and described herein as new to science; Pipistrellus
hesperidus for which Madagascar animals are genetically close but distinct from African populations of the same
species; and endemic P. raceyi, which shows segregation of eastern (mesic) and western (dry) populations and its
sister species relationships are unresolved. While the external and craniodental measurements, as well as bioacoustics variables, allow only partial differentiation of these six species of Vespertilioninae, molecular characters provide
definitive separation of the taxa, as do male bacular morphology.Irene D. Pritzker Foundation associated with the Field Museum of Natural History African Training Fund; John D and Catherine T.MacArthur Foundation; Volkswagen Foundation; and the Centre de Recherche et de Veille sur les maladies émergentes dans l’Océan Indien (CRVOI) through the Fonds Européen de Développement Régional Programme
Opérationnel de Coopération Territoriale Réunion, pathogènes associés à la faune sauvage ocean Indien #31189. SYNTHESYS project http://synthesys.info/, which is financed by European Community Research Infrastructure Action under the FP7
Integrating Activities Program.http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1096-36422016-04-30hb2015Zoology and EntomologyMammal Research Institut
Developing and piloting location-specific fertilizer recommendation for wheat in Ethiopia
This report covers the progress of developing and piloting location-specific fertilizer and agroclimate advisory service for Wheat in Ethiopia. The report highlights the major activities implemented during the 2022 cropping season including (1) review of organic fertilizer recommendations for wheat; (2) generation of location-specific fertilizer rate (LSFR) for piloting; (3) developing kebele map for LSFR recommendation; (4) video preparation for demo training; (5) trainings organized on piloting LSFR advisory and map reading; (6) LSFR advisory reach and adoption; (7) Alliance and partners pilot trial field visits during the main wheat season; (8) field days organized ; and (9) certificate of appreciation awarded to Alliance and its partners. The main purpose of the report is to narrative the processes associated with the development of agroadvisory and its piloting in the highlands of Ethiopia
Identification of Tenrec ecaudatus, a wild mammal Introduced to Mayotte Island, as a reservoir of the newly identified human pathogenic Leptospira mayottensis
Leptospirosis is a bacterial zoonosis of major concern on tropical islands. Human populations
on western Indian Ocean islands are strongly affected by the disease although each
archipelago shows contrasting epidemiology. For instance, Mayotte, part of the Comoros
Archipelago, differs from the other neighbouring islands by a high diversity of Leptospira
species infecting humans that includes Leptospira mayottensis, a species thought to be
unique to this island. Using bacterial culture, molecular detection and typing, the present
study explored the wild and domestic local mammalian fauna for renal carriage of leptospires
and addressed the genetic relationships of the infecting strains with local isolates
obtained from acute human cases and with Leptospira strains hosted by mammal species
endemic to nearby Madagascar. Tenrec (Tenrec ecaudatus, Family Tenrecidae), a terrestrial
mammal introduced from Madagascar, is identified as a reservoir of L. mayottensis. All
isolated L. mayottensis sequence types form a monophyletic clade that includes Leptospira
strains infecting humans and tenrecs on Mayotte, as well as two other Malagasy endemic
tenrecid species of the genus Microgale. The lower diversity of L. mayottensis in tenrecs
from Mayotte, compared to that occurring in Madagascar, suggests that L. mayottensis has
indeed a Malagasy origin. This study also showed that introduced rats (Rattus rattus) and
dogs are probably the main reservoirs of Leptospira borgpetersenii and Leptospira kirschneri,
both bacteria being prevalent in local clinical cases. Data emphasize the epidemiological
link between the two neighbouring islands and the role of introduced small mammals in
shaping the local epidemiology of leptospirosis.S1 Fig. Distribution of small mammals sampling sites on Mayotte. Numbers correspond to
the 18 sampling sites where Rattus rattus and Tenrec ecaudatus (white) and bats (orange) were
trapped. Map was created with QGIS 2.8.1 (QGIS Development Team, 2016, QGIS Geographic
Information System, Open Source Geospatial Foundation Project). Photography of Mayotte:
BD Topo IGN, 2008.S2 Fig. The figures A and B display Bayesian phylogenetic trees of pathogenic Leptospira
from Mayotte (blue) and Madagascar (green) based on 452 bp (57 taxa, HKY+I+G) (A) and
245 bp (64 taxa, K80+I) (B) of the rrs2 gene. At the nodes, the black numbers indicate posterior
probabilities. The sequences highlighted in grey (A) and red (B) represent PCR positive
samples for which only the rrs2 gene was obtained. Strain numbers of cultures produced herein
are indicated in parentheses, “K” and “U” designating sequences obtained from kidney or
urine, respectively. Specimen system: MDI and MAY = CRVOI specimen catalogue during
field trips to Mayotte; all Canis lupus familiaris were sampled during field trips to Mayotte;
FMNH = Field Museum of Natural History, Chicago; UADBA = Université d’Antananarivo,
Département de Biologie Animale, Madagascar; for the other bacterial sequences from Homo
sapiens and Microgale spp. see Bourhy et al. 2012 [15]and Dietrich et al. 2014 [4]. Museum
numbers for Microgale spp.: 575 = UADBA 30869; 588 = UADBA 30289; 590 = UADBA
30291; 1335 = UADBA 32122; 1453 = UADBA 32125; 1467 = UADBA 32101.ERDF-POCT Réunion, LeptOI (#32913), ParamyxOI
(#33857); Centre National de la Recherche
Scientifique - Institut Ecologie et Environnement
(ECOSAN BatMan) and Agence Régionale de la
Santé Ocean Indien.http://www.plosntds.orgam2016Medical Virolog
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A Prospective Study of Causes of Illness and Death in Preterm Infants in Ethiopia: The SIP Study Protocol
Background
With nearly 15 million annual preterm births globally, preterm birth is the most common cause of neonatal death. Forty to 60 % of neonatal deaths are directly or indirectly associated with preterm mortality. As countries aim to meet the Sustainable Development Goals to reduce neonatal mortality, significant reductions in preterm mortality are needed. This study aims to identify the common causes of preterm illness and their contribution to preterm mortality in low-resource settings. This article will describe the methods used to undertake the study.
Methods
This is a prospective, multi-centre, descriptive clinical study. Socio-demographic, obstetric, and maternal factors, and clinical and laboratory findings will be documented. The major causes of preterm mortality will be identified using clinical, laboratory, imaging, and autopsy methods and use the national Ethiopian guidelines on management of preterm infants including required investigations to reach final diagnoses. The study will document the clinical and management protocols followed in these settings. The approach consists of clinical examinations and monitoring, laboratory investigations, and determination of primary and contributory causes of mortality through both clinical means and by post-mortem examinations. An independent panel of experts will validate the primary and contributory causes of mortality.
To obtain the estimated sample size of 5000 preterm births, the study will be undertaken in five hospitals in three regions of Ethiopia, which are geographically distributed across the country. All preterm infants who are either born or transferred to these hospitals will be eligible for the study. Three methods (last menstrual period, physical examination using the New Ballard Score, and ultrasound) will be used to determine gestational age.
All clinical procedures will be conducted per hospital protocol and informed consent will be taken from parents or caretakers prior to their participation in the study as well as for autopsy if the infant dies.
Discussion
This study will determine the major causes of death and illness among hospitalized preterm infants in a low-resource setting. The result will inform policy makers and implementers of areas that can be prioritized in order to contribute to a significant reduction in neonatal mortality
Biogeography of Leptospira in wild animal communities inhabiting the insular ecosystem of the western Indian Ocean islands and neighboring Africa
Understanding the processes driving parasite assemblages is particularly important in the context of zoonotic infectious diseases. Leptospirosis is a widespread zoonotic bacterial infection caused by pathogenic species of the genus Leptospira. Despite a wide range of animal hosts, information is still lacking on the factors shaping Leptospira diversity in wild animal communities, especially in regions, such as tropical insular ecosystems, with high host species richness and complex biogeographical patterns. Using a large dataset (34 mammal species) and a multilocus approach at a regional scale, we analyzed the role of both host species diversity and geography in Leptospira genetic diversity in terrestrial small mammals (rodents, tenrecs, and shrews) and bats from 10 different islands/countries in the western Indian Ocean (WIO) and neighboring Africa. At least four Leptospira spp. (L. interrogans, L. borgpetersenii, L. kirschneri, and L. mayottensis) and several yet-unidentified genetic clades contributed to a remarkable regional Leptospira diversity, which was generally related to the local occurrence of the host species rather than the geography. In addition, the genetic structure patterns varied between Leptospira spp., suggesting different evolutionary histories in the region, which might reflect both in situ diversification of native mammals (for L. borgpetersenii) and the more recent introduction of non-native host species (for L. interrogans). Our data also suggested that host shifts occurred between bats and rodents, but further investigations are needed to determine how host ecology may influence these events
Diversity, host specialization, and geographic structure of filarial nematodes infecting Malagasy bats
We investigated filarial infection in Malagasy bats to gain insights into the diversity of these parasites and explore the factors shaping their distribution. Samples were obtained from 947 individual bats collected from 52 sites on Madagascar and representing 31 of the 44 species currently recognized on the island. Samples were screened for the presence of micro-and macro-parasites through both molecular and morphological approaches. Phylogenetic analyses showed that filarial diversity in Malagasy bats formed three main groups, the most common represented by Litomosa spp. infecting Miniopterus spp. (Miniopteridae); a second group infecting Pipistrellus cf. hesperidus (Vespertilionidae) embedded within the Litomosoides cluster, which is recognized herein for the first time from Madagascar; and a third group composed of lineages with no clear genetic relationship to both previously described filarial nematodes and found in M. griveaudi, Myotis goudoti, Neoromicia matroka (Vespertilionidae), Otomops madagascariensis (Molossidae), and Paratriaenops furculus (Hipposideridae). We further analyzed the infection rates and distribution pattern of Litomosa spp., which was the most diverse and prevalent filarial taxon in our sample. Filarial infection was disproportionally more common in males than females in Miniopterus spp., which might be explained by some aspect of roosting behavior of these cave-dwelling bats. We also found marked geographic structure in the three Litomosa clades, mainly linked to bioclimatic conditions rather than host-parasite associations. While this study demonstrates distinct patterns of filarial nematode infection in Malagasy bats and highlights potential drivers of associated geographic distributions, future work should focus on their alpha taxonomy and characterize arthropod vectors
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