Microbial Resources and Innovation in the Wine Production Sector

Microbial starter cultures represent a fundamental level of innovation in the wine sector. Selected yeast strains are routinely used to achieve the needed biomass preparation to accelerate and steer alcoholic fermentation in grape must. The use of starter cultures to induce malolactic fermentation in wine relies on the characterisation and propagation of suitable strains of lactic acid bacteria. Furthermore, the selection of new strains, the renewal of management of microbial resources and new technologies allow continuous improvements in oenology, which may increase the beneficial aspects of wine. In this review, with the aim to stimulate microbial-driven, consumer-oriented advances in the oenological sector, we propose an overview of recent trends in this field that are reported by following the classical separation into 'product innovation' and 'process innovation'. Hence, we shall highlight i) the possible positive innovative impacts of microbial resources on the safety and the sensorial and functional properties of wine (product innovation) and ii) the potential microbial-based improvements allowing the reduction of time/costs and the environmental impacts associated with winemaking (process innovation)

A bibliometric overview of University-business collaboration between 1980 and 2016

Bibliometrics is a research field that analyses bibliographic material from a quantitative point of view. Aiming at providing a comprehensive overview, this study scrutinises the academic literature in university business collaboration and technology transfer research for the period post the Bayh-Dole Act (1980-2016). The study employs the Web of Science as the main database from where information is collected. Bibliometric indicators such as number of publications, citations, productivity, and the H-index are used to analyse the results. The main findings are displayed in the form of tables and are further discussed. The focus is on the identification of the most relevant journals in this area, the most cited papers, most prolific authors, leading institutions, and countries. The results show that the USA, England, Spain, Italy, and the Netherlands are highly active in this area. Scientific production tends to fall within the research areas of business and economics, engineering or public administration, and is mainly published in journals such as Research Policy, Technovation and Journal of Technology Transfer.Peer ReviewedPostprint (published version

Examining strategies behind universities’ technology transfer portfolio: how different patterns of resource consumption can lead to similar technology transfer profiles

Purpose – This research analyses how different patterns of production factors consumption of Spanish universities lead to specific technology transfer (TT) profiles (outcomes). Design/methodology/approach – Adopting a resource-based view perspective (RBV), qualitative comparative analysis (QCA) is used to analyse the relationship between different combinations of resources—human resources, financial resources and support services—and various portfolios of TT outcomes—intellectual or industrial property agreements, spin-offs and TT income. Findings – Results indicate that there is no unique formula of resource consumption that leads to a specific portfolio of TT outcomes. These results seem to reflect the characteristics and competences added by universities, along with the characteristics of their socioeconomic context. From a RVB perspective, this indicates that the considered resources are substitutable. Practical implications – The effectiveness of university policies is expected to vary by university, e.g. depending on the type of resources that is most relevant in the university’s production process. To develop competitive advantages Spanish public universities must resort to internal intangible resources or specific and inimitable combinations of the available resources. Social implications – Since Spanish universities are heterogeneous and display different TT portfolios they address the needs of different users. Originality/value – Previous studies have failed to acknowledge the heterogeneity among universities. To perform our analysis we employed QCA, an innovative methodology in the higher education sector that enables us to purposefully acknowledge institutional diversity (in both resources and results). This allows us to indirectly take into account the capabilities of universities using a more holistic approach to evaluate their competitiveness.Peer ReviewedPostprint (published version

PTR-ToF-MS for the online monitoring of alcoholic fermentation in wine: assessment of VOCs variability associated with different combinations of Saccharomyces/non-Saccharomyces as a case-study

7openInternationalItalian coauthor/editorThe management of the alcoholic fermentation (AF) in wine is crucial to shaping product quality. Numerous variables (e.g., grape varieties, yeast species/strains, technological parameters) can affect the performances of this fermentative bioprocess. The fact that these variables are often interdependent, with a high degree of interaction, leads to a huge ‘oenological space’ associated with AF that scientists and professionals have explored to obtain the desired quality standards in wine and to promote innovation. This challenge explains the high interest in approaches tested to monitor this bioprocess including those using volatile organic compounds (VOCs) as target molecules. Among direct injection mass spectrometry approaches, no study has proposed an untargeted online investigation of the diversity of volatiles associated with the wine headspace. This communication proposed the first application of proton-transfer reaction-mass spectrometry coupled to a time-of-flight mass analyzer (PTR-ToF-MS) to follow the progress of AF and evaluate the impact of the different variables of wine quality. As a case study, the assessment of VOC variability associated with different combinations of Saccharomyces/non-Saccharomyces was selected. The different combinations of microbial resources in wine are among the main factors susceptible to influencing the content of VOCs associated with the wine headspaces. In particular, this investigation explored the effect of multiple combinations of two Saccharomyces strains and two non-Saccharomyces strains (belonging to the species Metschnikowia pulcherrima and Torulaspora delbrueckii) on the content of VOCs in wine, inoculated both in commercial grape juice and fresh grape must. The results demonstrated the possible exploitation of non-invasive PTR-ToF-MS monitoring to explore, using VOCs as biomarkers, (i) the huge number of variables influencing AF in wine, and (ii) applications of single/mixed starter cultures in wine. Reported preliminary findings underlined the presence of different behaviors on grape juice and on must, respectively, and confirmed differences among the single yeast strains ‘volatomes’. It was one of the first studies to include the simultaneous inoculation on two non-Saccharomyces species together with a S. cerevisiae strain in terms of VOC contribution. Among the other outcomes, evidence suggests that the addition of M. pulcherrima to the coupled S. cerevisiae/T. delbrueckii can modify the global release of volatiles as a function of the characteristics of the fermented matrixopenBerbegal, C.; Khomenko, I.; Russo, P.; Spano, G.; Fragasso, M.; Biasioli, F.; Capozzi, V.Berbegal, C.; Khomenko, I.; Russo, P.; Spano, G.; Fragasso, M.; Biasioli, F.; Capozzi, V

Variables associated with a higher awareness of gender-based violence by students of the health sciences and social work

Objective To determine the attitudes towards equality and prevention of gender-based violence, and analyze variables associated with a higher awareness of gender-based violence by students of the health sciences and social work degrees. Method A cross-sectional descriptive study was carried out. The sample consisted of 437 students of the health sciences and social work degrees at the University of Zaragoza (Spain) during 2018 and 2019. The variables of the study were: Socio-demographic variables, academic variables, feminism attitudes towards women''s movement using Feminism and the women''s movement scale (FWMS), attitudes on gender-based violence using the Gender Equality and Prevention of Gender-based Violence Scale (GEPGVS). A correlational study and multiple linear regression were performed, in order to analyze the associated variables. Results Differences in attitudes towards gender-based violence were observed according to sex, age and attitudes in line with the feminist movement. Regarding the linear regression model, the results showed that the FWMS is a predictor of GEPGVS, as well as sex. Conclusions Holding attitudes in line with the feminist movement is a factor that may be promoted in order to increase the awareness of gender-based violence. Objetivo Determinar las actitudes hacia la igualdad y la sensibilización en cuanto a la violencia de género, y analizar las variables asociadas a una mayor sensibilización sobre la violencia de género de los estudiantes de ciencias de la salud y trabajo social. Método Se realizó un estudio descriptivo transversal. La muestra consistió en 437 estudiantes de los grados de ciencias de la salud y trabajo social de la Universidad de Zaragoza (España) durante 2018 y 2019. Las variables del estudio fueron variables sociodemográficas, variables académicas, actitudes hacia el movimiento feminista usando la Feminism and the women''s movement scale (FWMS) y actitudes hacia la violencia de género mediante la Escala sobre Igualdad y Prevención de la Violencia de Género (EIPVG). Se realizaron un estudio correlacional y una regresión lineal múltiple para conocer las variables asociadas a una mayor sensibilización. Resultados Se observaron diferencias en las actitudes hacia la violencia de género en función del sexo, la edad y las actitudes favorables al movimiento feminista. Con respecto al modelo de regresión lineal, los resultados mostraron que la FWMS es un predictor de la EIPVG, así como el sexo. Conclusiones Las actitudes favorables al movimiento feminista son un factor que puede promoverse para incrementar la sensibilización sobre la violencia de género

Complex molecular relationship between vegetative compatibility groups (VCGs) in Verticillium dahliae: VCGs do not always align with clonal lineages

Verticillium wilts caused by the soilborne fungus Verticillium dahliae are among the most challenging diseases to control. Populations of this pathogen have been traditionally studied by means of vegetative compatibility groups (VCGs) under the assumption that VCGs comprise genetically related isolates that correlate with clonal lineages. We aimed to resolve the phylogenetic relationships among VCGs and their subgroups based on sequences of the intergenic spacer region (IGS) of the ribosomal DNA and six anonymous polymorphic sequences containing single-nucleotide polymorphisms (VdSNPs). A collection of 68 V dahliae isolates representing the main VCGs and subgroups (VCGs 1A, 1B, 2A, 2B, 3, 4A, 4B, and 6) from different geographic origins and hosts was analyzed using the seven DNA regions. Maximum parsimony (MP) phylogenies inferred from IGS and VdSNP sequences showed five and six distinct clades, respectively. Phylogenetic analyses of individual and combined data sets indicated that certain VCG subgroups (e.g., VCGs 1A and 1B) are closely related and share a common ancestor; however, other subgroups (e.g., VCG 4B) are more closely related to members of a different VCG (e.g., VCG 2A) than to subgroups of the same VCG (VCG 4B). Furthermore, MP analyses indicated that VCG 2B is polyphyletic, with isolates placed in at least three distinct phylogenetic lineages based on IGS sequences and two lineages based on VdSNP sequences. Results from our study suggest the existence of main VCG lineages that contain VCGs 1A and 1B; VCGs 2A and 4B; and VCG 4A, for which both phylogenies agree; and the existence of other VCGs or VCG subgroups that seem to be genetically heterogeneous or show discrepancies in their phylogenetic placement: VCG 2B, VCG 3, and VCG 6. These results raise important caveats regarding the interpretation of VCG analyses: genetic homogeneity and close evolutionary relationship between members of a VCG should not be assumed.This research was partially funded by the Sarah Chinn Kalser Faculty Research Assistance Endowment, College of Agricultural Sciences, The Pennsylvania State University. We thank all suppliers of V. dahliae isolates; J. Yanez, S. Colihan, C. Barrett, C. Black, and C. Olivares-Garcia for excellent technical support; and D. Geiser for helpful discussions during the preparation of this manuscript.Jiménez Gasco, MDM.; Malcolm, GM.; Berbegal Martinez, M.; Armengol Fortí, J.; Jimenez Diaz, R. (2014). Complex molecular relationship between vegetative compatibility groups (VCGs) in Verticillium dahliae: VCGs do not always align with clonal lineages. Phytopathology. 104(6):650-659. doi:10.1094/PHYTO-07-13-0180-RS650659104

Guía sobre las enfermedades fúngicas de la madera de la vid

Reunión Grupo de Trabajo de experimentación en viticultura y enología. 34ª Reunión: Centro de Transferencia Agroalimentaria: Gobierno de Aragón: Zaragoza: 10 y 11 de abril de 2019. ANEXOLas enfermedades fúngicas de la madera de la vid (EMV) son uno de los retos más importantes que actualmente debe afrontar la viticultura en todo el mundo para su sostenibilidad. Estas enfermedades causan daños severos en el viñedo todos los años y su incidencia está creciendo rápidamente en todos los países productores (De la Fuente et al., 2016). Las EMV están causadas por varios hongos patógenos que viven y colonizan la madera de los órganos perennes, causando necrosis y decoloración de la madera, infecciones vasculares, y descomposición esponjosa seca. Las viñas afectadas muestran, externamente, un decaimiento general y progresivo (retraso de la brotación, yemas muertas, decaimiento de la planta, parada del desarrollo, clorosis, apoplejía, etc.), generalmente asociado a unos síntomas foliares acordes a las diferentes enfermedades, que inicialmente pueden causar pérdida de productividad y eventualmente la muerte de las plantas (Gramaje et al., 2018). En España se estima una media del 3% de plantas afectadas por EMV, aunque existen plantaciones recién establecidas en las que su incidencia podría superar el 20%, generando pérdidas económicas anuales muy elevadas (De la Fuente et al., 2016). Según estimaciones recientes, el coste anual de replantar el 1% de vid del cultivar Tempranillo en La Rioja debido a las EMV asciende a 7,16 millones de euros anuales (Martínez-Diz et al., 2019). En Francia, se estima que aproximadamente un 12% de los viñedos son improductivos debido a las EMV, causando unas pérdidas anuales de 1 billón de euros (Lorch, 2014)

First report of Alternaria black spot of pomegranate caused by Alternaria alternata in Spain

Berbegal, M.; López- Cortés, I.; Salazar Hernández, DM.; Gramaje, D.; Perez-Sierra, A.; Garcia-Jimenez, J.; Armengol, J. (2014). First report of Alternaria black spot of pomegranate caused by Alternaria alternata in Spain. Plant Disease. 98(5):689-689. doi:10.1094/PDIS-07-13-0717-PDNS68968998