Effect of processing parameters on recovery of hot process virgin coconut oil and co-products utilization

Virgin coconut oil (VCO) is growing in popularity as functional food, cosmetic and pharmaceutical oil. The high cost of VCO is due to its low recovery. In order to improve the recovery, the effect of milk expelling methods (manual and mechanical) and pre- treatments (slicing, pulverizing and blanching) on coconut milk and hot process VCO recovery with respect to fresh coconut kernel weight was studied. The blanching and pulverizing yielded more milk and VCO recovery in both manual and mechanical expelling methods. The recovery of coconut milk and VCO ranged from 34.0 to 51.6 per cent and 14.2 to 22.4 per cent respectively. Among the different treatment combinations, pulverized, blanched and double screw pressed coconut kernel yielded the highest milk and VCO recovery. The per cent recovery of two important co-products namely coconut milk residue and VCO cake ranged from 38.5 to 55.6 and 6.3 to 8.8 respectively. An attempt was made to recover the oil from 8 per cent dried coconut milk residue and VCO cake in commercial oil expeller. The oil recovery from milk residue and VCO cake flour was 41.2±1.1 per cent and 25.8±1.0 per cent respectively. The dried coconut milk residue and VCO cake flour was utilized in the preparation of extrudates and sweet snacks along with the broken rice, maize grits and pearl millet grits

Deciphering mollusc shell production: the roles of genetic mechanisms through to ecology, aquaculture and biomimetics

Most molluscs possess shells, constructed from a vast array of microstructures and architectures. The fully formed shell is composed of calcite or aragonite. These CaCO3 crystals form complex biocomposites with proteins, which although typically less than 5% of total shell mass, play significant roles in determining shell microstructure. Despite much research effort, large knowledge gaps remain in how molluscs construct and maintain their shells, and how they produce such a great diversity of forms. Here we synthesize results on how shell shape, microstructure, composition and organic content vary among, and within, species in response to numerous biotic and abiotic factors. At the local level, temperature, food supply and predation cues significantly affect shell morphology, whilst salinity has a much stronger influence across latitudes. Moreover, we emphasize how advances in genomic technologies [e.g. restriction site-associated DNA sequencing (RAD-Seq) and epigenetics] allow detailed examinations of whether morphological changes result from phenotypic plasticity or genetic adaptation, or a combination of these. RAD-Seq has already identified single nucleotide polymorphisms associated with temperature and aquaculture practices, whilst epigenetic processes have been shown significantly to modify shell construction to local conditions in, for example, Antarctica and New Zealand. We also synthesize results on the costs of shell construction and explore how these affect energetic trade-offs in animal metabolism. The cellular costs are still debated, with CaCO3 precipitation estimates ranging from 1-2 J/mg to 17-55 J/mg depending on experimental and environmental conditions. However, organic components are more expensive (~29 J/mg) and recent data indicate transmembrane calcium ion transporters can involve considerable costs. This review emphasizes the role that molecular analyses have played in demonstrating multiple evolutionary origins of biomineralization genes. Although these are characterized by lineage-specific proteins and unique combinations of co-opted genes, a small set of protein domains have been identified as a conserved biomineralization tool box. We further highlight the use of sequence data sets in providing candidate genes for in situ localization and protein function studies. The former has elucidated gene expression modularity in mantle tissue, improving understanding of the diversity of shell morphology synthesis. RNA interference (RNAi) and clustered regularly interspersed short palindromic repeats - CRISPR-associated protein 9 (CRISPR-Cas9) experiments have provided proof of concept for use in the functional investigation of mollusc gene sequences, showing for example that Pif (aragonite-binding) protein plays a significant role in structured nacre crystal growth and that the Lsdia1 gene sets shell chirality in Lymnaea stagnalis. Much research has focused on the impacts of ocean acidification on molluscs. Initial studies were predominantly pessimistic for future molluscan biodiversity. However, more sophisticated experiments incorporating selective breeding and multiple generations are identifying subtle effects and that variability within mollusc genomes has potential for adaption to future conditions. Furthermore, we highlight recent historical studies based on museum collections that demonstrate a greater resilience of molluscs to climate change compared with experimental data. The future of mollusc research lies not solely with ecological investigations into biodiversity, and this review synthesizes knowledge across disciplines to understand biomineralization. It spans research ranging from evolution and development, through predictions of biodiversity prospects and future-proofing of aquaculture to identifying new biomimetic opportunities and societal benefits from recycling shell products.FCT: UID/Multi/04326/2019; European Marine Biological Research Infrastructure Cluster-EMBRIC (EU H2020 research and innovation program) 654008; European Union Seventh Framework Programme [FP7] ITN project 'CACHE: Calcium in a Changing Environment' under REA 60505; NERC Natural Environment Research Council NE/J500173/1info:eu-repo/semantics/publishedVersio

Soluble perlecan domain i enhances vascular endothelial growth factor-165 activity and receptor phosphorylation in human bone marrow endothelial cells

<p>Abstract</p> <p>Background</p> <p>Immobilized recombinant perlecan domain I (PlnDI) binds and modulates the activity of heparin-binding growth factors, <it>in vitro</it>. However, activities for PlnDI, in solution, have not been reported. In this study, we assessed the ability of soluble forms to modulate vascular endothelial growth factor-165 (VEGF₁₆₅) enhanced capillary tube-like formation, and VEGF receptor-2 phosphorylation of human bone marrow endothelial cells, <it>in vitro</it>.</p> <p>Results</p> <p>In solution, PlnDI binds VEGF₁₆₅in a heparan sulfate and pH dependent manner. Capillary tube-like formation is enhanced by exogenous PlnDI; however, PlnDI/VEGF₁₆₅mixtures combine to enhance formation beyond that stimulated by either PlnDI or VEGF₁₆₅alone. PlnDI also stimulates VEGF receptor-2 phosphorylation, and mixtures of PlnDI/VEGF₁₆₅reduce the time required for peak VEGF receptor-2 phosphorylation (Tyr-951), and increase Akt phosphorylation. PlnDI binds both immobilized neuropilin-1 and VEGF receptor-2, but has a greater affinity for neuropilin-1. PlnDI binding to neuropilin-1, but not to VEGF receptor-2 is dependent upon the heparan sulfate chains adorning PlnDI. Interestingly, the presence of VEGF₁₆₅but not VEGF₁₂₁significantly enhances PlnDI binding to Neuropilin-1 and VEGF receptor-2.</p> <p>Conclusions</p> <p>Our observations suggest soluble forms of PlnDI are biologically active. Moreover, PlnDI heparan sulfate chains alone or together with VEGF₁₆₅can enhance VEGFR-2 signaling and angiogenic events, <it>in vitro</it>. We propose PlnDI liberated during basement membrane or extracellular matrix turnover may have similar activities, <it>in vivo</it>.</p

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Not AvailableDragon fruit (Hylocereus spp.), a herbaceous perennial climbing cactus, widely known as Red Pitaya, has recently drawn much attention among the Indian growers, not only because of its attractive red or pink color and economic value as fruit, but also valued for its high antioxidant potential, vitamins and minerals content. Being a native of Southern Mexico, Guatemala and Costa Rica, dragon fruit was introduced in India during the late `90s and still the area under its cultivation is gradually increasing. Farmers in the Indian states of Karnataka, Kerala, Tamil Nadu, Maharashtra, Gujarat, Orissa, West Bengal, Andhra Pradesh and Andaman & Nicobar Islands have already taken up its cultivation, and the estimated total area under Dragon fruit cultivation in these regions may be less than 400 ha. Majority of the dragon fruits presently available in Indian markets are imported from Vietnam, Thailand, Malaysia and Sri Lanka. Being a cactus family and requires long day for flowering, dragon fruit cultivation is well suited in the agro-climatic regions of Southern, Western and North Eastern India that are dry and frost-free. Due to high demand both in domestic and international markets, dragon fruit production could be an economical avocation to both backyard growers as well as entrepreneurs of medium and large scale plantations. Cultivation of dragon fruit already started in different parts of India with many success stories of farmer from different regions. Many nursery men started propagation for raising planting material of dragon fruit. One of the widely grown cultivars and most commonly available dragon fruit is the red color epicarp with white and pink pulped sub-sweet juicy pulp matrix. It is a fast return perennial fruit crop with economic production in the first year after planting, and full production within three to five years. It was also noted to initially produce in its first years on wards provided desirable cultural management practices are applied. Although the initial investment is relatively high, profit is substantial within 4-5 years. The red flesh species i.e H. costaricensis are additionally rich in betalains, meeting the increasing trade interest for antioxidant products and natural food colorant. Fruits are easy to keep fresh under room condition. The crop is hardy and can survive in any type of climatic condition favorable for flowering and fruiting and soil condition provided with good drainage.Not Availabl

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Optimization of methodology for the extraction of polyphenolic compounds with antioxidant potential and α-glucosidase inhibitory activity from Jamun (Syzygium cumini L.) seeds

Jamun (Syzygium cumini&nbsp;L.) seed is one of the rich sources of polyphenolic compounds​ ​with antioxidant potential and α-glucosidase inhibitory activity. A study was conducted to​ ​optimize the methodology for the extraction of polyphenolic compounds (total phenolic​ ​and flavonoid contents) with antioxidant potential and α-glucosidase inhibitory activity​ ​from Jamun seed powder. The study showed that the nature of solvent and extraction​ ​conditions had a significant effect on total phenolic content (TPC), total flavonoid content​ ​(TFC), antioxidant potential, and α-glucosidase inhibitory activity. The TPC varied between​ ​6.0 (mg/g Jamun seed powder) for the acetone extract to 119.2 (mg/g) for 80% aqueous​ ​acetone extract and TFC varied between 1.06 mg/g for the acetone to 10.81 mg/g for the​ ​80% aqueous methanol. From the study, it was apparent that an aqueous form of acetone​ ​(acetone: water 80:20, v/v) is a better solvent system for extraction of polyphenolic​ ​compounds with high antioxidant potential and α-glucosidase inhibitory activity.​ ​Ultrasonication for 60 min increased the efficiency of phenolic extraction

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Not AvailableVirgin coconut oil (VCO) is growing in popularity as functional food, cosmetic and pharmaceutical oil. The high cost of VCO is due to its low recovery. In order to improve the recovery, the effect of milk expelling methods (manual and mechanical) and pretreatments (slicing, pulverizing and blanching) on coconut milk and hot process VCO recovery with respect to fresh coconut kernel weight was studied. The blanching and pulverizing yielded more milk and VCO recovery in both manual and mechanical expelling methods. The recovery of coconut milk and VCO ranged from 34.0 to 51.6 per cent and 14.2 to 22.4 per cent respectively. Among the different treatment combinations, pulverized, blanched and double screw pressed coconut kernel yielded the highest milk and VCO recovery. The per cent recovery of two important co-products namely coconut milk residue and VCO cake ranged from 38.5 to 55.6 and 6.3 to 8.8 respectively. An attempt was made to recover the oil from 8 per cent dried coconut milk residue and VCO cake in commercial oil expeller. The oil recovery from milk residue and VCO cake flour was 41.2±1.1 per cent and 25.8±1.0 per cent respectively. The dried coconut milk residue and VCO cake flour was utilized in the preparation of extrudates and sweet snacks along with the broken rice, maize grits and pearl millet grits.Not Availabl

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Not AvailableTrigonella foenum‑graecum, commonly called fenugreek, is a leguminous plant native to many Asian, Middle Eastern and European countries. Fenugreek oil is very effective in digestion. Identification of fenugreek genotype rich in saponins and fixed oil will be useful for pharmaceutical industries. In the present study, steroidal saponin and fixed oil content was analysed in 46 diverse fenugreek genotypes on dry weight basis. Significant differences were observed in the total saponin and fixed oil content among the genotypes. Saponin and fixed oil content ranged from 0.92 g to 1.68 g and 3.25 to 6.88 g with corresponding mean value of 1.34 g and 5.19 g/100 g dw, respectively.ICA