Perspectives on the role of teacher aides and the implications for inclusive practice in Aotearoa classrooms : a thesis presented in partial fulfilment of the requirements for the degree of Master of Education (Inclusive Education) at Massey University, Albany, New Zealand

Every young person has the right to experience school, learning alongside their peers, building friendships with classmates and gaining a sense of autonomy. Teacher aides have traditionally been placed in classrooms to support students with disabilities and diverse learning and behavioural differences. An overreliance on teacher aide support, however, may lead to students becoming stigmatised and dependent. Through thematic analysis of interview data gathered from six teachers and eight teacher aides, supplemented by survey responses from 23 teachers 14 teacher aides, this thesis examines the perspectives on the teacher aide role and the implications for inclusive practice in Aotearoa classrooms. It identifies four prevalent role types perceived by participants, the teacher aides as: an aide to the teacher; a coeducator; a student aide (in class); and a student aide (outside class). Some roles, particularly the co-educator, are more conducive to social interaction and facilitating students’ independence. Others, particularly the student aide role, risk further isolating particular students. This thesis argues that it is teachers rather than teacher aides who are the primary agents of inclusive practice. As teachers adapt their practice to ensure that learning and achievement are possible for every student in their class, the need for one-on-one teacher aide support can be reduced. Teacher aides can work alongside teachers as co-educators overseeing the entire class, instructing small groups and checking-in with various students as needed, enabling teachers to work with students who benefit from more nuanced instruction. School leaders must examine the roles they assign to teacher aides and the associated practices in schools and classrooms. This will ensure that teacher aides are not viewed as the sole mechanisms for instructing and caring for students with disabilities and other diversities. Teacher aides are valuable members of the school community and can play a key role in contributing to inclusive practice in Aotearoa classrooms

Nanodiamond preparation and surface characterization for biological applications

Nanodiamonds contain stable fluorescent emitters and hence can be used for molecular fluorescence imaging and precision sensing of electromagnetic fields. The physical properties of these emitters together with their low reported cytotoxicity make them attractive for biological imaging applications. The controlled application of nanodiamonds for cellular imaging requires detailed understanding of surface chemistry, size ranges and aggregation, as these can all influence cellular interactions. We compared these characteristics for graphitic and oxidized nanodiamonds. Oxidation is generally used for surface functionalization, and was optimized by Thermogravimetric Analysis, achieved by 445±5°C heating in air for 5 hours, then confirmed via Raman and Infrared spectroscopies. Size ranges and aggregation were assessed using Atomic Force Microscopy and Dynamic Light Scattering. Biocompatibility in breast cancer cell lines was measured using a proliferation assay. Heating at 445±5°C reduced the Raman signal of graphitic carbon (1575 cm-1) as compared to that of diamond (1332 cm-1) from 0.31±0.07 Raman intensity units to 0.07±0.04. This temperature was substantially below the onset of major mass loss (observed at 535±1°C) and therefore achieved cost efficiency, convenience and high yield. Graphitic and oxidized nanodiamonds formed aggregates in water, with a mean particle size of 192±4nm and 166±2nm at a concentration of 66μg/mL. We then applied the graphitic and oxidized nanodiamonds to cells in culture at 1μg/mL and found no significant change in the proliferation rate (-5±2% and -1±3% respectively). Nanodiamonds may therefore be suitable for development as a novel transformative tool in the life sciences

Vertebrate Hosts as Islands: Dynamics of Selection, Immigration, Loss, Persistence, and Potential Function of Bacteria on Salamander Skin.

Skin bacterial communities can protect amphibians from a fungal pathogen; however, little is known about how these communities are maintained. We used a neutral model of community ecology to identify bacteria that are maintained on salamanders by selection or by dispersal from a bacterial reservoir (soil) and ecological drift. We found that 75% (9/12) of bacteria that were consistent with positive selection, <1% of bacteria that were consistent with random dispersal and none of the bacteria that were consistent under negative selection had a 97% or greater match to antifungal isolates. Additionally we performed an experiment where salamanders were either provided or denied a bacterial reservoir and estimated immigration and loss (emigration and local extinction) rates of bacteria on salamanders in both treatments. Loss was strongly related to bacterial richness, suggesting competition is important for structuring the community. Bacteria closely related to antifungal isolates were more likely to persist on salamanders with or without a bacterial reservoir, suggesting they had a competitive advantage. Furthermore, over-represented and under-represented operational taxonomic units (OTUs) had similar persistence on salamanders when a bacterial reservoir was present. However, under-represented OTUs were less likely to persist in the absence of a bacterial reservoir, suggesting that the over-represented and under-represented bacteria were selected against or for on salamanders through time. Our findings from the neutral model, migration and persistence analyses show that bacteria that exhibit a high similarity to antifungal isolates persist on salamanders, which likely protect hosts against pathogens and improve fitness. This research is one of the first to apply ecological theory to investigate assembly of host associated-bacterial communities, which can provide insights for probiotic bioaugmentation as a conservation strategy against disease

Raman micro-spectroscopy for accurate identification of primary human bronchial epithelial cells.

Live cell Raman micro-spectroscopy is emerging as a promising bioanalytical technique for label-free discrimination of a range of different cell types (e.g. cancer cells and fibroblasts) and behaviors (e.g. apoptosis). The aim of this study was to determine whether confocal Raman micro-spectroscopy shows sufficient sensitivity and specificity for identification of primary human bronchial epithelial cells (HBECs) to be used for live cell biological studies in vitro. We first compared cell preparation substrates and media, considering their influence on lung cell proliferation and Raman spectra, as well as methods for data acquisition, using different wavelengths (488 nm, 785 nm) and scan protocols (line, area). Evaluating these parameters using human lung cancer (A549) and fibroblast (MRC5) cell lines confirmed that line-scan data acquisition at 785 nm using complete cell media on a quartz substrate gave optimal performance. We then applied our protocol to acquisition of data from primary human bronchial epithelial cells (HBEC) derived from three independent sources, revealing an average sensitivity for different cell types of 96.3% and specificity of 95.2%. These results suggest that Raman micro-spectroscopy is suitable for delineating primary HBEC cell cultures, which in future could be used for identifying different lung cell types within co-cultures and studying the process of early carcinogenesis in lung cell culture

Mend the Gap: The Independent Review into Gender Pay Gaps in Medicine in England

The Independent Review into Gender Pay Gaps in Medicine in England was commissioned by the Department of Health and Social Care in 2017. It is the largest and most comprehensive review of its kind ever completed in the public sector. Chaired by Professor Dame Jane Dacre and led by Professor Carol Woodhams, the review takes a comprehensive approach to understanding the structural and cultural barriers affecting the female medical workforce

A Descriptive Analysis of the Temporal and Geographical Proximities Seen Within UK Series of Sex Offenses

Previous studies of the geographical and temporal features of serial sex offenses are limited by small samples and/or geographical areas, and are dated. We address a significant gap in the literature by investigating the temporal and geographical proximity of the crimes of 402 serial stranger sex offenders in the UK. Periods of incarceration were extracted from calculations of temporal proximity giving a more accurate picture of series duration and time elapsed between offenses from the same series. A notable minority of serial stranger sex offenders commit their offenses within very close geographic proximity and the same was found for temporal proximity. There were also occurrences of series spanning large distances and many years. The implications of these findings for the use of geography and time in the behavioral linking of crimes, and what they mean for policy decisions regarding financial investment in law enforcement technology, are discussed

Heterogeneous presynaptic distribution of monoacylglycerol lipase, a multipotent regulator of nociceptive circuits in the mouse spinal cord.

Monoacylglycerol lipase (MGL) is a multifunctional serine hydrolase, which terminates anti-nociceptive endocannabinoid signaling and promotes pro-nociceptive prostaglandin signaling. Accordingly, both acute nociception and its sensitization in chronic pain models are prevented by systemic or focal spinal inhibition of MGL activity. Despite its analgesic potential, the neurobiological substrates of beneficial MGL blockade have remained unexplored. Therefore, we examined the regional, cellular and subcellular distribution of MGL in spinal circuits involved in nociceptive processing. All immunohistochemical findings obtained with light, confocal or electron microscopy were validated in MGL-knockout mice. Immunoperoxidase staining revealed a highly concentrated accumulation of MGL in the dorsal horn, especially in superficial layers. Further electron microscopic analysis uncovered that the majority of MGL-immunolabeling is found in axon terminals forming either asymmetric glutamatergic or symmetric gamma-aminobutyric acid/glycinergic synapses in laminae I/IIo. In line with this presynaptic localization, analysis of double-immunofluorescence staining by confocal microscopy showed that MGL colocalizes with neurochemical markers of peptidergic and non-peptidergic nociceptive terminals, and also with markers of local excitatory or inhibitory interneurons. Interestingly, the ratio of MGL-immunolabeling was highest in calcitonin gene-related peptide-positive peptidergic primary afferents, and the staining intensity of nociceptive terminals was significantly reduced in MGL-knockout mice. These observations highlight the spinal nociceptor synapse as a potential anatomical site for the analgesic effects of MGL blockade. Moreover, the presence of MGL in additional terminal types raises the possibility that MGL may play distinct regulatory roles in synaptic endocannabinoid or prostaglandin signaling according to its different cellular locations in the dorsal horn pain circuitry